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The map label for this gene is gmuC [H]

Identifier: 226222934

GI number: 226222934

Start: 331866

End: 333179

Strand: Reverse

Name: gmuC [H]

Synonym: Lm4b_00326

Alternate gene names: 226222934

Gene position: 333179-331866 (Counterclockwise)

Preceding gene: 226222935

Following gene: 226222933

Centisome position: 11.44

GC content: 42.47

Gene sequence:

>1314_bases
ATGCGATTTAATACCATTAGCGAAAAAATGGATCAATATATTTCCCCGCTGGCTAACAAACTTAGCCAGCAACGCCATTT
GAAAGCAACCAGAGATGCCTTTATGTCCATGCTGCCAATTACATTGTTTGGTTCAATTCCGATTATTTTGAAAGCGGCTC
CAGTAACAGATGATACGAAAAACGGCTTCCTGCTTGCCTGGGCTAACTTCGCTGAAAAATATGATTTAATCCTAAATTGG
ATTAGCGGAATTACACTTGGCGCCATGTCGTTATATATTTGCGTCGGTATCACGTATTATTTATGCAAACACTACCACGA
AGATTTCTTACGCCCACTCATGTTTTCTATCGCCGGTTTCTTTATGCTCGTACTTAACCCAATCAAAATGGGTTGGGACG
GCAAAGAAGTCGACATCAGTTTCCTTGATGGACGAGGAATATTACTCTCGATTTTCGTCGCCATCGTTACCGTAGAAGGT
TATCACTGGATGCGCAAAAAAAATGTCGGTCGAATAACCATGCCCGATAGCGTCCCAGCCTCACTATCCGAGACATTCGC
AGCCTTAGTTCCTGGTATTATTTTAATGACCTTTTTCTCGATTATCTTTATCATTTTCAACCTACTAGACACAACAGCCA
TCCAATTCTTATATGAAAAAATGGCGCCGAGCTTCAAAGCAGCCGATAGCTTACCATTCACGATTCTAAGTATTTTCCTA
GTTCACTTATTCTGGTTCTTCGGTGTACACGATGCCGCACTTGCCGGAATTTTGGGCCCAATTCGTGACGGAAACCTTTC
TATCAACGCCGCTGAAAAAATGGCTGGTCAAGATTTGACGAATATTTTCACGACACCATTCTGGACATATTTCGTTATCA
TCGGTGGTTCTGGTTCCGTTCTCGCACTTGCCTTTTTAATGATGCGATCCAAGTCCAAACAACTAAGCACCGTCGGGAAA
GTCGGCTTCTTACCTTCCATATTCGGAATTTCTGAGCCGCTAATTTTCGGAACACCACTAATGCTGAATCCGATTTTCTT
CTTCCCGTTCATTTTCACATCCGTATTCAATGGTGTCATCACGTATCTTTGTATGTATTTCGGGATTGTCGGAAAAACGT
TCGCCGTCTTCTCCTGGCAAATGCCCGCACCAATTGGAGCATTTCTATCCACCATGGACTGGCGAGCCATCGTTCTCGTA
TTCATCCTGATTTTCTTAAACGGCCTAATGTACTACCCATTCCTAAAAGTGTATGAGAAAAACCTTGTCGCTTTAGAAAA
AGAAGCCGAAGAAGAAAACATCGCTGCTAATTAA

Upstream 100 bases:

>100_bases
GTCAGCGTCCCAGTCCATGCCATTTCACCAGTAAAATACGGCATCATGGACGGCAAAGGGGTTTACGAAGACATCCAGAA
ATTAATAGGAGGATAAAAAC

Downstream 100 bases:

>100_bases
TAACCACTTAAATAGAGATTGGGACAAAAAAACTCGTCTCTTTCTCTATTTGGTGTTGTATAATAACAATAGGTTAAGGG
AGAATTAGGGGGGATATAAT

Product: PTS beta-glucoside-specific enzyme IIC component

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: Glucomannan utilization protein C; PTS system oligo-beta-mannoside-specific EIIC component [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MRFNTISEKMDQYISPLANKLSQQRHLKATRDAFMSMLPITLFGSIPIILKAAPVTDDTKNGFLLAWANFAEKYDLILNW
ISGITLGAMSLYICVGITYYLCKHYHEDFLRPLMFSIAGFFMLVLNPIKMGWDGKEVDISFLDGRGILLSIFVAIVTVEG
YHWMRKKNVGRITMPDSVPASLSETFAALVPGIILMTFFSIIFIIFNLLDTTAIQFLYEKMAPSFKAADSLPFTILSIFL
VHLFWFFGVHDAALAGILGPIRDGNLSINAAEKMAGQDLTNIFTTPFWTYFVIIGGSGSVLALAFLMMRSKSKQLSTVGK
VGFLPSIFGISEPLIFGTPLMLNPIFFFPFIFTSVFNGVITYLCMYFGIVGKTFAVFSWQMPAPIGAFLSTMDWRAIVLV
FILIFLNGLMYYPFLKVYEKNLVALEKEAEEENIAAN

Sequences:

>Translated_437_residues
MRFNTISEKMDQYISPLANKLSQQRHLKATRDAFMSMLPITLFGSIPIILKAAPVTDDTKNGFLLAWANFAEKYDLILNW
ISGITLGAMSLYICVGITYYLCKHYHEDFLRPLMFSIAGFFMLVLNPIKMGWDGKEVDISFLDGRGILLSIFVAIVTVEG
YHWMRKKNVGRITMPDSVPASLSETFAALVPGIILMTFFSIIFIIFNLLDTTAIQFLYEKMAPSFKAADSLPFTILSIFL
VHLFWFFGVHDAALAGILGPIRDGNLSINAAEKMAGQDLTNIFTTPFWTYFVIIGGSGSVLALAFLMMRSKSKQLSTVGK
VGFLPSIFGISEPLIFGTPLMLNPIFFFPFIFTSVFNGVITYLCMYFGIVGKTFAVFSWQMPAPIGAFLSTMDWRAIVLV
FILIFLNGLMYYPFLKVYEKNLVALEKEAEEENIAAN
>Mature_437_residues
MRFNTISEKMDQYISPLANKLSQQRHLKATRDAFMSMLPITLFGSIPIILKAAPVTDDTKNGFLLAWANFAEKYDLILNW
ISGITLGAMSLYICVGITYYLCKHYHEDFLRPLMFSIAGFFMLVLNPIKMGWDGKEVDISFLDGRGILLSIFVAIVTVEG
YHWMRKKNVGRITMPDSVPASLSETFAALVPGIILMTFFSIIFIIFNLLDTTAIQFLYEKMAPSFKAADSLPFTILSIFL
VHLFWFFGVHDAALAGILGPIRDGNLSINAAEKMAGQDLTNIFTTPFWTYFVIIGGSGSVLALAFLMMRSKSKQLSTVGK
VGFLPSIFGISEPLIFGTPLMLNPIFFFPFIFTSVFNGVITYLCMYFGIVGKTFAVFSWQMPAPIGAFLSTMDWRAIVLV
FILIFLNGLMYYPFLKVYEKNLVALEKEAEEENIAAN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=453, Percent_Identity=29.3598233995585, Blast_Score=168, Evalue=6e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 49021; Mature: 49021

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFNTISEKMDQYISPLANKLSQQRHLKATRDAFMSMLPITLFGSIPIILKAAPVTDDTK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
NGFLLAWANFAEKYDLILNWISGITLGAMSLYICVGITYYLCKHYHEDFLRPLMFSIAGF
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMLVLNPIKMGWDGKEVDISFLDGRGILLSIFVAIVTVEGYHWMRKKNVGRITMPDSVPA
HHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHCCCCEEECCCCCCH
SLSETFAALVPGIILMTFFSIIFIIFNLLDTTAIQFLYEKMAPSFKAADSLPFTILSIFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
VHLFWFFGVHDAALAGILGPIRDGNLSINAAEKMAGQDLTNIFTTPFWTYFVIIGGSGSV
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHCCCHHHHHHHCCHHHEEEEECCCCHH
LALAFLMMRSKSKQLSTVGKVGFLPSIFGISEPLIFGTPLMLNPIFFFPFIFTSVFNGVI
HHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHH
TYLCMYFGIVGKTFAVFSWQMPAPIGAFLSTMDWRAIVLVFILIFLNGLMYYPFLKVYEK
HHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLVALEKEAEEENIAAN
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRFNTISEKMDQYISPLANKLSQQRHLKATRDAFMSMLPITLFGSIPIILKAAPVTDDTK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
NGFLLAWANFAEKYDLILNWISGITLGAMSLYICVGITYYLCKHYHEDFLRPLMFSIAGF
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMLVLNPIKMGWDGKEVDISFLDGRGILLSIFVAIVTVEGYHWMRKKNVGRITMPDSVPA
HHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHCCCCEEECCCCCCH
SLSETFAALVPGIILMTFFSIIFIIFNLLDTTAIQFLYEKMAPSFKAADSLPFTILSIFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
VHLFWFFGVHDAALAGILGPIRDGNLSINAAEKMAGQDLTNIFTTPFWTYFVIIGGSGSV
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHCCCHHHHHHHCCHHHEEEEECCCCHH
LALAFLMMRSKSKQLSTVGKVGFLPSIFGISEPLIFGTPLMLNPIFFFPFIFTSVFNGVI
HHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHH
TYLCMYFGIVGKTFAVFSWQMPAPIGAFLSTMDWRAIVLVFILIFLNGLMYYPFLKVYEK
HHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLVALEKEAEEENIAAN
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]