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The map label for this gene is cysS

Identifier: 226222868

GI number: 226222868

Start: 256083

End: 257498

Strand: Direct

Name: cysS

Synonym: Lm4b_00259

Alternate gene names: 226222868

Gene position: 256083-257498 (Clockwise)

Preceding gene: 226222867

Following gene: 226222869

Centisome position: 8.79

GC content: 40.32

Gene sequence:

>1416_bases
TTGTCGATACAAATTTTTAATACGTTAAAACGAGAGAAAGAGCCTTTTAAACCACTCAAAGATGGCGAAGTGAAAATGTA
CGTTTGCGGACCGACTGTGTATAACTACATTCATATCGGGAATGCGCGGCCAATTATTGTTTTCGATACAGTTCGACGTT
ATTTTACGTATCGTGGTTATGATGTGAAATTCGTGTCTAATTTTACGGATGTGGATGATAAATTGATTCGTGCGGCAAAT
GAGCTAAAGTTGACGGTTCCCGAAGTGGCGGATCGTTTTATTGGGGCTTACTTTGATGATGTAGATCAACTGAATGTGGC
GAAAGCGACTGTGAATCCACGTGTAACGGAGAATATGGATGAAATTATTCAATTGATCAGTACGCTAATTGAAAAAGGCT
ATGCGTATGAGTCGGCTGGTGATGTGTATTTCCGGACGAAAAAATTCAAAGATTACGGGAAACTATCTGGCCAAGAATTA
TCGGAATTGCAGCACGGGGCTCGGGTTGAATATAATGAGCGTAAGCAAGATGAACTTGATTTCACCCTTTGGAAAGCGGC
TAAACCGGGTGAAATTTTCTGGGAAAGTCCGTTTGGTAATGGGCGCCCGGGTTGGCATATTGAATGCTCGGCGCTTGCGA
AAAAATATCTTGGTGACACGATTGATATTCATGCGGGTGGGCAAGATTTAGTATTTCCTCACCATGAGGATGAGATTGCT
CAATCCGAAGCGGCAACTGGGAAAACGTTCGCGAACTACTGGATGCATAATGCCTTTTTAAATATTGATGGGGAAAAAAT
GTCCAAATCACTTGGGAATTTTATTACGCTGCATGATGTGCTAAAAGACAATGATCCAAATGTGATTCGTTTCTTCATGC
TATCTGTACATTACCGGAAGCCAATTACACTGAATGATGCAATTTTAGAAGATGCGAAAAATGGTTTGGAACGGTTAATG
ATTGCATACCAAAATATTGATCATCGTATTCAAACAGATGACGGTGAATATGTGGAAGAAGCGCATGAGGACGAGTGGCT
AGAACAGCTAACGGAATTAAAACAAGCTTTTGAAGATGATATGGATGATGATTTCAATACGGCAAATGCAATTACTACTT
TTCACGAACTTGCAAAGCGTGCCAATATTTATTTGGCTAAAGAAACTGTTTCTATCAATGTTCTGCGCGAATTCTTAAGC
ATGATGCGTTTGTTTGCGGAAGTATTAGGCTTGAAATTAGAAAATACGCAAACTGATTCGCTGGATGATAGCGAAGTAGA
AGCGTTAATTGAAGAACGTCTGCAAGCGCGAAACGAACGTAATTTTGCACGTGCTGATGAAATTCGAGACATTTTAAAAG
AAAAAAATATTATTTTGGAAGACACTGCGCAAGGCACGCGGTTTAGACGGGGGTAA

Upstream 100 bases:

>100_bases
GGTAGGACATGCGGTTCCTAAAATGGATGAACTGACATTACGAATTGCGGAATTAGAAAATATAGTAGAAAAACTTTTGA
AGGAAAAGGAGTAGATCTGA

Downstream 100 bases:

>100_bases
ATCATGGCAGAAGTGAAAGAATACAAACAACTTAATGGCCTCGCGCTTGCTTACATGGGTGACGCAGTTTATGAAAAATT
TATTCGCGAATATTTACTTG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSIQIFNTLKREKEPFKPLKDGEVKMYVCGPTVYNYIHIGNARPIIVFDTVRRYFTYRGYDVKFVSNFTDVDDKLIRAAN
ELKLTVPEVADRFIGAYFDDVDQLNVAKATVNPRVTENMDEIIQLISTLIEKGYAYESAGDVYFRTKKFKDYGKLSGQEL
SELQHGARVEYNERKQDELDFTLWKAAKPGEIFWESPFGNGRPGWHIECSALAKKYLGDTIDIHAGGQDLVFPHHEDEIA
QSEAATGKTFANYWMHNAFLNIDGEKMSKSLGNFITLHDVLKDNDPNVIRFFMLSVHYRKPITLNDAILEDAKNGLERLM
IAYQNIDHRIQTDDGEYVEEAHEDEWLEQLTELKQAFEDDMDDDFNTANAITTFHELAKRANIYLAKETVSINVLREFLS
MMRLFAEVLGLKLENTQTDSLDDSEVEALIEERLQARNERNFARADEIRDILKEKNIILEDTAQGTRFRRG

Sequences:

>Translated_471_residues
MSIQIFNTLKREKEPFKPLKDGEVKMYVCGPTVYNYIHIGNARPIIVFDTVRRYFTYRGYDVKFVSNFTDVDDKLIRAAN
ELKLTVPEVADRFIGAYFDDVDQLNVAKATVNPRVTENMDEIIQLISTLIEKGYAYESAGDVYFRTKKFKDYGKLSGQEL
SELQHGARVEYNERKQDELDFTLWKAAKPGEIFWESPFGNGRPGWHIECSALAKKYLGDTIDIHAGGQDLVFPHHEDEIA
QSEAATGKTFANYWMHNAFLNIDGEKMSKSLGNFITLHDVLKDNDPNVIRFFMLSVHYRKPITLNDAILEDAKNGLERLM
IAYQNIDHRIQTDDGEYVEEAHEDEWLEQLTELKQAFEDDMDDDFNTANAITTFHELAKRANIYLAKETVSINVLREFLS
MMRLFAEVLGLKLENTQTDSLDDSEVEALIEERLQARNERNFARADEIRDILKEKNIILEDTAQGTRFRRG
>Mature_470_residues
SIQIFNTLKREKEPFKPLKDGEVKMYVCGPTVYNYIHIGNARPIIVFDTVRRYFTYRGYDVKFVSNFTDVDDKLIRAANE
LKLTVPEVADRFIGAYFDDVDQLNVAKATVNPRVTENMDEIIQLISTLIEKGYAYESAGDVYFRTKKFKDYGKLSGQELS
ELQHGARVEYNERKQDELDFTLWKAAKPGEIFWESPFGNGRPGWHIECSALAKKYLGDTIDIHAGGQDLVFPHHEDEIAQ
SEAATGKTFANYWMHNAFLNIDGEKMSKSLGNFITLHDVLKDNDPNVIRFFMLSVHYRKPITLNDAILEDAKNGLERLMI
AYQNIDHRIQTDDGEYVEEAHEDEWLEQLTELKQAFEDDMDDDFNTANAITTFHELAKRANIYLAKETVSINVLREFLSM
MRLFAEVLGLKLENTQTDSLDDSEVEALIEERLQARNERNFARADEIRDILKEKNIILEDTAQGTRFRRG

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI13375695, Length=480, Percent_Identity=37.7083333333333, Blast_Score=278, Evalue=6e-75,
Organism=Homo sapiens, GI303304987, Length=419, Percent_Identity=31.7422434367542, Blast_Score=188, Evalue=9e-48,
Organism=Homo sapiens, GI21269877, Length=419, Percent_Identity=31.7422434367542, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI62240992, Length=419, Percent_Identity=31.7422434367542, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI10835051, Length=419, Percent_Identity=31.7422434367542, Blast_Score=187, Evalue=1e-47,
Organism=Escherichia coli, GI1786737, Length=478, Percent_Identity=44.5606694560669, Blast_Score=388, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17509795, Length=327, Percent_Identity=33.9449541284404, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17509797, Length=327, Percent_Identity=33.9449541284404, Blast_Score=176, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6324082, Length=356, Percent_Identity=35.3932584269663, Blast_Score=196, Evalue=6e-51,
Organism=Drosophila melanogaster, GI19922218, Length=505, Percent_Identity=32.8712871287129, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI19922364, Length=324, Percent_Identity=32.4074074074074, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e [H]

EC number: =6.1.1.16 [H]

Molecular weight: Translated: 54380; Mature: 54249

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQIFNTLKREKEPFKPLKDGEVKMYVCGPTVYNYIHIGNARPIIVFDTVRRYFTYRGY
CCEEHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEHHHHHHHHHCCC
DVKFVSNFTDVDDKLIRAANELKLTVPEVADRFIGAYFDDVDQLNVAKATVNPRVTENMD
EEEEECCCCCHHHHHHHHHHHCEEEHHHHHHHHHHHHHCCHHHCCHHHEECCCHHHCCHH
EIIQLISTLIEKGYAYESAGDVYFRTKKFKDYGKLSGQELSELQHGARVEYNERKQDELD
HHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCC
FTLWKAAKPGEIFWESPFGNGRPGWHIECSALAKKYLGDTIDIHAGGQDLVFPHHEDEIA
EEEECCCCCCCEEEECCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHH
QSEAATGKTFANYWMHNAFLNIDGEKMSKSLGNFITLHDVLKDNDPNVIRFFMLSVHYRK
HHHHCCCHHHHHHHHHHHEEECCHHHHHHHHCCEEEEHHHHCCCCCCCEEHEEHHHHCCC
PITLNDAILEDAKNGLERLMIAYQNIDHRIQTDDGEYVEEAHEDEWLEQLTELKQAFEDD
CCEEHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC
MDDDFNTANAITTFHELAKRANIYLAKETVSINVLREFLSMMRLFAEVLGLKLENTQTDS
CCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCC
LDDSEVEALIEERLQARNERNFARADEIRDILKEKNIILEDTAQGTRFRRG
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
SIQIFNTLKREKEPFKPLKDGEVKMYVCGPTVYNYIHIGNARPIIVFDTVRRYFTYRGY
CEEHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEHHHHHHHHHCCC
DVKFVSNFTDVDDKLIRAANELKLTVPEVADRFIGAYFDDVDQLNVAKATVNPRVTENMD
EEEEECCCCCHHHHHHHHHHHCEEEHHHHHHHHHHHHHCCHHHCCHHHEECCCHHHCCHH
EIIQLISTLIEKGYAYESAGDVYFRTKKFKDYGKLSGQELSELQHGARVEYNERKQDELD
HHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCC
FTLWKAAKPGEIFWESPFGNGRPGWHIECSALAKKYLGDTIDIHAGGQDLVFPHHEDEIA
EEEECCCCCCCEEEECCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHH
QSEAATGKTFANYWMHNAFLNIDGEKMSKSLGNFITLHDVLKDNDPNVIRFFMLSVHYRK
HHHHCCCHHHHHHHHHHHEEECCHHHHHHHHCCEEEEHHHHCCCCCCCEEHEEHHHHCCC
PITLNDAILEDAKNGLERLMIAYQNIDHRIQTDDGEYVEEAHEDEWLEQLTELKQAFEDD
CCEEHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC
MDDDFNTANAITTFHELAKRANIYLAKETVSINVLREFLSMMRLFAEVLGLKLENTQTDS
CCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCC
LDDSEVEALIEERLQARNERNFARADEIRDILKEKNIILEDTAQGTRFRRG
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]