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The map label for this gene is plcB

Identifier: 226222834

GI number: 226222834

Start: 208014

End: 208883

Strand: Direct

Name: plcB

Synonym: Lm4b_00203

Alternate gene names: 226222834

Gene position: 208014-208883 (Clockwise)

Preceding gene: 226222833

Following gene: 226222835

Centisome position: 7.14

GC content: 36.21

Gene sequence:

>870_bases
GTGAAGTTCAAAAAAGTGGTTCTAGGTATGTGCTTGACCGCAAGTGTTCTAGTCTTTCCTGTAACGATAAAAGCAAGTGC
CTGTTGTGATGAATATCTAAAGCCCCCCGCAGCTCCGCATGATATTGACAGCAAATTACCGCATAAACTTAGTTGGTCCG
CGGATAATCCGACAAATACTGACGTAAATACGCACTATTGGCTTTTTAAACAAGCAGAAAAAATACTAGCTAAAGATGTA
GATCATATGCGAGCTAATTTAATGAATGAACTTAAAAATTTCGACAAACAAATTGCTCAAGGAATATATGACGCGGATCA
TAAAAATCCATATTATGATACTAGTACGTTTTTATCTCATTTTTATAATCCTGATAAAGATAATACTTATTTGCCAGGTT
TTGCTAATGCGAAAATAACAGGAGCCAAGTATTTTAATCAATCGGTGGCTGATTACCGAGAAGGGAAATTTGACACAGCA
TTTTATAAATTGGGCCTAGCAATCCATTATTATACGGATATTAGTCAACCTATGCACGCCAATAATTTTACCGCAATATC
CTATCCACCAGGCTACCACTGTGCATATGAAAATTACGTAGATACCATTAAACACAATTATCAAGCAACAGAAGACATGG
TAGTGCAAAGATTTTGCTCAAATGACGTGAAAGAATGGCTCTATGAAAATGCGAAAAGGGCGAAAGCGGACTATCCGAAA
ATAGTCAATGCGAAAACTAAAAAATCATACTTAGTAGGAAATTCCGAATGGAAAAAGGATACAGTGGAACCTACTGGAGC
TAGACTAAGAGATTCACAGCAAACTTTGGCAGGCTTTTTAGAATTTTGGTCTAAAAAAACAAATGAATAA

Upstream 100 bases:

>100_bases
TAGCTATTGGCGTGTTCTCTTTAGGAGCGGTTATCAAAATCATTCAATTAAGAAAAAATAGTTGAAGACGCAGAATGAAA
GAAAAAGTGAGGTGAGTGAT

Downstream 100 bases:

>100_bases
CAATATTTAGGAATATATTCTTATCCACTCGTTAGCGGGTGGATATATTTTATGGGGAGGAAGTAAGCCGAATGTATATA
AAAGGGAGGTTAATCTTTTT

Product: phospholipase C

Products: NA

Alternate protein names: PLC; Lecithinase; Phosphatidylcholine cholinephosphohydrolase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKFKKVVLGMCLTASVLVFPVTIKASACCDEYLKPPAAPHDIDSKLPHKLSWSADNPTNTDVNTHYWLFKQAEKILAKDV
DHMRANLMNELKNFDKQIAQGIYDADHKNPYYDTSTFLSHFYNPDKDNTYLPGFANAKITGAKYFNQSVADYREGKFDTA
FYKLGLAIHYYTDISQPMHANNFTAISYPPGYHCAYENYVDTIKHNYQATEDMVVQRFCSNDVKEWLYENAKRAKADYPK
IVNAKTKKSYLVGNSEWKKDTVEPTGARLRDSQQTLAGFLEFWSKKTNE

Sequences:

>Translated_289_residues
MKFKKVVLGMCLTASVLVFPVTIKASACCDEYLKPPAAPHDIDSKLPHKLSWSADNPTNTDVNTHYWLFKQAEKILAKDV
DHMRANLMNELKNFDKQIAQGIYDADHKNPYYDTSTFLSHFYNPDKDNTYLPGFANAKITGAKYFNQSVADYREGKFDTA
FYKLGLAIHYYTDISQPMHANNFTAISYPPGYHCAYENYVDTIKHNYQATEDMVVQRFCSNDVKEWLYENAKRAKADYPK
IVNAKTKKSYLVGNSEWKKDTVEPTGARLRDSQQTLAGFLEFWSKKTNE
>Mature_289_residues
MKFKKVVLGMCLTASVLVFPVTIKASACCDEYLKPPAAPHDIDSKLPHKLSWSADNPTNTDVNTHYWLFKQAEKILAKDV
DHMRANLMNELKNFDKQIAQGIYDADHKNPYYDTSTFLSHFYNPDKDNTYLPGFANAKITGAKYFNQSVADYREGKFDTA
FYKLGLAIHYYTDISQPMHANNFTAISYPPGYHCAYENYVDTIKHNYQATEDMVVQRFCSNDVKEWLYENAKRAKADYPK
IVNAKTKKSYLVGNSEWKKDTVEPTGARLRDSQQTLAGFLEFWSKKTNE

Specific function: Important role in the infectious process. May contribute to efficient lysis of the two-membrane vacuoles that surround the bacteria after direct cell-to-cell spread [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Zn-dependent PLC domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008947
- InterPro:   IPR001531
- ProDom:   PD003946 [H]

Pfam domain/function: PF00882 Zn_dep_PLPC [H]

EC number: =3.1.4.3 [H]

Molecular weight: Translated: 33205; Mature: 33205

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS00384 PROKAR_ZN_DEPEND_PLPC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKKVVLGMCLTASVLVFPVTIKASACCDEYLKPPAAPHDIDSKLPHKLSWSADNPTNT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCC
DVNTHYWLFKQAEKILAKDVDHMRANLMNELKNFDKQIAQGIYDADHKNPYYDTSTFLSH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
FYNPDKDNTYLPGFANAKITGAKYFNQSVADYREGKFDTAFYKLGLAIHYYTDISQPMHA
HCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCCC
NNFTAISYPPGYHCAYENYVDTIKHNYQATEDMVVQRFCSNDVKEWLYENAKRAKADYPK
CCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
IVNAKTKKSYLVGNSEWKKDTVEPTGARLRDSQQTLAGFLEFWSKKTNE
HCCCCCHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKFKKVVLGMCLTASVLVFPVTIKASACCDEYLKPPAAPHDIDSKLPHKLSWSADNPTNT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCC
DVNTHYWLFKQAEKILAKDVDHMRANLMNELKNFDKQIAQGIYDADHKNPYYDTSTFLSH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
FYNPDKDNTYLPGFANAKITGAKYFNQSVADYREGKFDTAFYKLGLAIHYYTDISQPMHA
HCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCCC
NNFTAISYPPGYHCAYENYVDTIKHNYQATEDMVVQRFCSNDVKEWLYENAKRAKADYPK
CCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
IVNAKTKKSYLVGNSEWKKDTVEPTGARLRDSQQTLAGFLEFWSKKTNE
HCCCCCHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1309513; 1582425; 11679669; 1904842 [H]