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The map label for this gene is actA

Identifier: 226222833

GI number: 226222833

Start: 206164

End: 207978

Strand: Direct

Name: actA

Synonym: Lm4b_00202

Alternate gene names: 226222833

Gene position: 206164-207978 (Clockwise)

Preceding gene: 226222832

Following gene: 226222834

Centisome position: 7.08

GC content: 40.11

Gene sequence:

>1815_bases
GTGGGATTAAATAGATTTATGCGTGCGATGATGGTAGTTTTCATTACCGCCAACTGCATTACGATTAACCCCGACATAAT
ATTTGCAGCGACAGATAGCGAAGATTCCAGTCTGAACACAGATGAATGGGAAGAAGAAAAAACAGAAGAGCAGCCAAGCG
AGGTAAATACGGGACCAAGATACGAAACTGCACGTGAAGTAAGTTCACGTGATATTGAGGAACTAGAAAAATCGAATAAA
GTGAAAAATACGAACAAAGCAGACCTAATAGCAATGTTGAAAGCAAAAGCAGAAAAAGGTCCAAATATTAATAATAACAA
CAGTGAACAAAGCGAGAATGTGGCTATAAATGAAGAAGCTTCAGGATCCGACCGACCAGCTATACAAGTGGAGCGTCGTC
ATCCAGGGTTGCCATCGGATAGCGCCGCGGAAATTAAAAAAAGAAGGAAAGCTATAGCATCATCGGATAGTGAGCTTGAA
AGCCTTACTTATCCGGATAAACCAACAAAAGCAACTAAGAAAAAAGTGGCGAAAGCGTCAGTTACGGATACTTCTGAAAG
TGACTTAGATTCTAGCATGCAGTCAGCGGATGAGTCTACACCACAACCTTTAAAAGCAAATCAACAACCATTTTTCCCTA
AAGTATTTAAAAAAATAAAAGATGCGGGTAAATGGGTACGTGATAAAATCGACGAAAATCCCGAAGTGAAGAAAGCAATT
GTTGATAAAAGTGCAGGGTTAATTGACCAATTATTAACTAAGAAGAAAAATGAAGAGGTAAATGCTTCGGACTTTCCACC
ACCGCCTACGGATGAAGAGTTAAGACTTGCTTTGCCAGAGACACCGATGCTTCTTGGTTTTAATGCTCCTGCTACATCGG
AACCGAGCTCATTCGAATTTCCACCGCCTCCAACAGAAGATGAACTAGAAATTATGCGGGAAACAGCACCTTCGCTAGAT
TCTAGTTTTACAAGCGGGGATTTAGCTAGTTTGAGAAGTGCTATTAATCGCCATAGCCAAAATTTCTCTGATTTTCCACC
AATGCCAACAGAAGAAGAGTTGAACGGGAGAGGTGGTATACCAACATCTGAAGAATTTAGTTCGCTGAATAGTGGCGATT
TTACAGATGACGAAAACAGCGAGACAACAGAAGAAGAAATTGATCGCCTAGCTGATTTAAGAGATAGAGGAACAGGAAAA
CACTCAAGAAATGCGGGTTTTTTACCATTAAATCCGTTTACTAGCAGCCCGGTTCCTTCGTTAAGTCCAAAGGTATCGAA
AATAAGCGCGCCGGCTCTGATAACTGACGTAACTAAAAAAGCACCATTTAAAAATCCGCCACAGCCATTAAATGTGTTTA
ACAAAAAAACTACAACGAAAACAGCACCAAAAAAAATAACCCCTGTGAATACCGCGCCAAAGCTAGCAGCACTTCCTATC
ACGAAAGCGCAAGAAACTGAGCTTGGGGAAAATAAAGCACCATTTATAGAAAAACAAGCAGAGACAAACAATCGGCCAAT
TGATATGCCGAGCCTACCAGTAATCCAAAAAGAAGTTACAGAAAGAAATAAAGAGGAAATGAAACCGCAAACCGAGGAAA
AAGTGGTAGGAGAAAGCGAACCAGCTAATAATGTGAACGGAAAAAAACGTTCTGCTGGCATTGAAGAAGGAAAATTAATT
GCTAAAAGTGCAGAAGACGAAAAAGCAAAAGAAGAACCAGTGAACCATACGACGTTAATTCTTGCCATGTTAGCTATTGG
CGTGTTCTCTTTAGGAGCGGTTATCAAAATCATTCAATTAAGAAAAAATAGTTGA

Upstream 100 bases:

>100_bases
TGCTAATCCAATTTTTAACGGAATAAATTAGTGAAAATGAAGGCCAGATTTTCCTTGTTCTAAAAAGGTTGTATTAGCGT
ATCACGAGGAGGGAGTATAA

Downstream 100 bases:

>100_bases
AGACGCAGAATGAAAGAAAAAGTGAGGTGAGTGATGTGAAGTTCAAAAAAGTGGTTCTAGGTATGTGCTTGACCGCAAGT
GTTCTAGTCTTTCCTGTAAC

Product: actin-assembly inducing protein precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MGLNRFMRAMMVVFITANCITINPDIIFAATDSEDSSLNTDEWEEEKTEEQPSEVNTGPRYETAREVSSRDIEELEKSNK
VKNTNKADLIAMLKAKAEKGPNINNNNSEQSENVAINEEASGSDRPAIQVERRHPGLPSDSAAEIKKRRKAIASSDSELE
SLTYPDKPTKATKKKVAKASVTDTSESDLDSSMQSADESTPQPLKANQQPFFPKVFKKIKDAGKWVRDKIDENPEVKKAI
VDKSAGLIDQLLTKKKNEEVNASDFPPPPTDEELRLALPETPMLLGFNAPATSEPSSFEFPPPPTEDELEIMRETAPSLD
SSFTSGDLASLRSAINRHSQNFSDFPPMPTEEELNGRGGIPTSEEFSSLNSGDFTDDENSETTEEEIDRLADLRDRGTGK
HSRNAGFLPLNPFTSSPVPSLSPKVSKISAPALITDVTKKAPFKNPPQPLNVFNKKTTTKTAPKKITPVNTAPKLAALPI
TKAQETELGENKAPFIEKQAETNNRPIDMPSLPVIQKEVTERNKEEMKPQTEEKVVGESEPANNVNGKKRSAGIEEGKLI
AKSAEDEKAKEEPVNHTTLILAMLAIGVFSLGAVIKIIQLRKNS

Sequences:

>Translated_604_residues
MGLNRFMRAMMVVFITANCITINPDIIFAATDSEDSSLNTDEWEEEKTEEQPSEVNTGPRYETAREVSSRDIEELEKSNK
VKNTNKADLIAMLKAKAEKGPNINNNNSEQSENVAINEEASGSDRPAIQVERRHPGLPSDSAAEIKKRRKAIASSDSELE
SLTYPDKPTKATKKKVAKASVTDTSESDLDSSMQSADESTPQPLKANQQPFFPKVFKKIKDAGKWVRDKIDENPEVKKAI
VDKSAGLIDQLLTKKKNEEVNASDFPPPPTDEELRLALPETPMLLGFNAPATSEPSSFEFPPPPTEDELEIMRETAPSLD
SSFTSGDLASLRSAINRHSQNFSDFPPMPTEEELNGRGGIPTSEEFSSLNSGDFTDDENSETTEEEIDRLADLRDRGTGK
HSRNAGFLPLNPFTSSPVPSLSPKVSKISAPALITDVTKKAPFKNPPQPLNVFNKKTTTKTAPKKITPVNTAPKLAALPI
TKAQETELGENKAPFIEKQAETNNRPIDMPSLPVIQKEVTERNKEEMKPQTEEKVVGESEPANNVNGKKRSAGIEEGKLI
AKSAEDEKAKEEPVNHTTLILAMLAIGVFSLGAVIKIIQLRKNS
>Mature_603_residues
GLNRFMRAMMVVFITANCITINPDIIFAATDSEDSSLNTDEWEEEKTEEQPSEVNTGPRYETAREVSSRDIEELEKSNKV
KNTNKADLIAMLKAKAEKGPNINNNNSEQSENVAINEEASGSDRPAIQVERRHPGLPSDSAAEIKKRRKAIASSDSELES
LTYPDKPTKATKKKVAKASVTDTSESDLDSSMQSADESTPQPLKANQQPFFPKVFKKIKDAGKWVRDKIDENPEVKKAIV
DKSAGLIDQLLTKKKNEEVNASDFPPPPTDEELRLALPETPMLLGFNAPATSEPSSFEFPPPPTEDELEIMRETAPSLDS
SFTSGDLASLRSAINRHSQNFSDFPPMPTEEELNGRGGIPTSEEFSSLNSGDFTDDENSETTEEEIDRLADLRDRGTGKH
SRNAGFLPLNPFTSSPVPSLSPKVSKISAPALITDVTKKAPFKNPPQPLNVFNKKTTTKTAPKKITPVNTAPKLAALPIT
KAQETELGENKAPFIEKQAETNNRPIDMPSLPVIQKEVTERNKEEMKPQTEEKVVGESEPANNVNGKKRSAGIEEGKLIA
KSAEDEKAKEEPVNHTTLILAMLAIGVFSLGAVIKIIQLRKNS

Specific function: Virulence factor required for host cell microfilament interaction. It induces actin assembly around the bacteria to allow it to move within the cytoplasm. It is involved in the actin polymerization process. It seems to act as a nucleator that induces the

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007752 [H]

Pfam domain/function: PF05058 ActA [H]

EC number: NA

Molecular weight: Translated: 66297; Mature: 66166

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLNRFMRAMMVVFITANCITINPDIIFAATDSEDSSLNTDEWEEEKTEEQPSEVNTGPR
CCHHHHHHHHHHHHHHCCEEEECCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
YETAREVSSRDIEELEKSNKVKNTNKADLIAMLKAKAEKGPNINNNNSEQSENVAINEEA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCC
SGSDRPAIQVERRHPGLPSDSAAEIKKRRKAIASSDSELESLTYPDKPTKATKKKVAKAS
CCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHH
VTDTSESDLDSSMQSADESTPQPLKANQQPFFPKVFKKIKDAGKWVRDKIDENPEVKKAI
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VDKSAGLIDQLLTKKKNEEVNASDFPPPPTDEELRLALPETPMLLGFNAPATSEPSSFEF
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCC
PPPPTEDELEIMRETAPSLDSSFTSGDLASLRSAINRHSQNFSDFPPMPTEEELNGRGGI
CCCCCHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCC
PTSEEFSSLNSGDFTDDENSETTEEEIDRLADLRDRGTGKHSRNAGFLPLNPFTSSPVPS
CCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCC
LSPKVSKISAPALITDVTKKAPFKNPPQPLNVFNKKTTTKTAPKKITPVNTAPKLAALPI
CCCCHHHHCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCC
TKAQETELGENKAPFIEKQAETNNRPIDMPSLPVIQKEVTERNKEEMKPQTEEKVVGESE
CCCHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCC
PANNVNGKKRSAGIEEGKLIAKSAEDEKAKEEPVNHTTLILAMLAIGVFSLGAVIKIIQL
CCCCCCCCHHHCCCCCCCEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RKNS
HCCC
>Mature Secondary Structure 
GLNRFMRAMMVVFITANCITINPDIIFAATDSEDSSLNTDEWEEEKTEEQPSEVNTGPR
CHHHHHHHHHHHHHHCCEEEECCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
YETAREVSSRDIEELEKSNKVKNTNKADLIAMLKAKAEKGPNINNNNSEQSENVAINEEA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCC
SGSDRPAIQVERRHPGLPSDSAAEIKKRRKAIASSDSELESLTYPDKPTKATKKKVAKAS
CCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHH
VTDTSESDLDSSMQSADESTPQPLKANQQPFFPKVFKKIKDAGKWVRDKIDENPEVKKAI
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VDKSAGLIDQLLTKKKNEEVNASDFPPPPTDEELRLALPETPMLLGFNAPATSEPSSFEF
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCC
PPPPTEDELEIMRETAPSLDSSFTSGDLASLRSAINRHSQNFSDFPPMPTEEELNGRGGI
CCCCCHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCC
PTSEEFSSLNSGDFTDDENSETTEEEIDRLADLRDRGTGKHSRNAGFLPLNPFTSSPVPS
CCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCC
LSPKVSKISAPALITDVTKKAPFKNPPQPLNVFNKKTTTKTAPKKITPVNTAPKLAALPI
CCCCHHHHCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCC
TKAQETELGENKAPFIEKQAETNNRPIDMPSLPVIQKEVTERNKEEMKPQTEEKVVGESE
CCCHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCC
PANNVNGKKRSAGIEEGKLIAKSAEDEKAKEEPVNHTTLILAMLAIGVFSLGAVIKIIQL
CCCCCCCCHHHCCCCCCCEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RKNS
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1309513; 1739966; 1582425; 11679669; 8112291 [H]