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The map label for this gene is holB [H]

Identifier: 226222795

GI number: 226222795

Start: 163041

End: 164033

Strand: Direct

Name: holB [H]

Synonym: Lm4b_00163

Alternate gene names: 226222795

Gene position: 163041-164033 (Clockwise)

Preceding gene: 226222793

Following gene: 226222796

Centisome position: 5.6

GC content: 41.69

Gene sequence:

>993_bases
GTGGGATTACAGGATGAACTTACAGTGATGCAACCAGTGGTCATGAAGATTTTTTCAAAAAGTGTCCGCGAAAATCGATT
ATCTCACGGCTATTTATTCGAAGGAGCAAGAGGTACAGGTAAGAAACGCACAGCACTTTGGTTAGCTCAGAGTCTTTTTT
GTTTAGAAAGCACTGAAACCGAGCTTGCTTGTGGGAAGTGTGCCAATTGCACGAGAATAGCAAGTCATAATCATCCGGAT
GTTCATTTGCTAGAGCCAGATGGAGCTAGTATTAAGATTGATCAGGTTCGCGCGCTGAAGCAAGAACTTAGTAAACGCGG
AATGGAATCAGATCAAAAAGTAGTTATCATTTACGATGCAGAAAAAATGACTGTTCAATCAGCCAATAGTCTGCTGAAAT
TTATAGAAGAACCAGAAGGCGGTTTGTTATTATTATTTTTAACAACAAACCCCGGGCAAATTTTGCCAACGATTCAATCA
AGACTGCAACCTGTCACATTCAAATCACTGACTTTTGATAGTTTACTCGCATCACTAACAGCAGCAGGCATTTCCGAACA
AAAAGCGCGGATTTATGCTAGCATTACTGGGAGTGTGGAAGAAGCGAAGGCTTTTGAAGAGAGTGAGTGGTTTAGTGAGG
CAAGAAATGTTGTCATCAAGTTATATGAAGGAATTCACCATCAAGGAACCAGCCCATTAATTATCATTCAAGAATCATGG
ATGCCACTTTTTAAAGAGAAAGATAAGATGGCGCTTGGGCTCGAATTATTGTTACTGCTGTACCGCGACCGGCTACACCT
TACACTTGACGAGAACTACGAACCTATTTGTACTGCGCAAAAAGAAATGCTCGGACAAGATGCGCTGCGTAAATCACTGT
CCGAAACCACAGGGGAAATCGAGAAGATTCTCGCTGCGAAATCAAAACTGGATTCCAATATGAACACGCAACTTCTAATG
GAGCAGTTAGTTCTGGAAATCCAAGGGAGGTAA

Upstream 100 bases:

>100_bases
TTAGTTAACCAATCCAACGTTATTTTTTTGTTAAAATTCCGAAGCTATTTAAGCTTGGTCATGGTATAATATTACAATAT
GGATAGAAAGGAAGTTTTTT

Downstream 100 bases:

>100_bases
TCGCTATGCTTAAAATTGTAGGCGTCCGTTTTAAAGACGTTGGTAAAATATATTATTTCTCACCTGGCGAACTGGAAATC
ACAGAAAACCAAGGTGTTAT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MGLQDELTVMQPVVMKIFSKSVRENRLSHGYLFEGARGTGKKRTALWLAQSLFCLESTETELACGKCANCTRIASHNHPD
VHLLEPDGASIKIDQVRALKQELSKRGMESDQKVVIIYDAEKMTVQSANSLLKFIEEPEGGLLLLFLTTNPGQILPTIQS
RLQPVTFKSLTFDSLLASLTAAGISEQKARIYASITGSVEEAKAFEESEWFSEARNVVIKLYEGIHHQGTSPLIIIQESW
MPLFKEKDKMALGLELLLLLYRDRLHLTLDENYEPICTAQKEMLGQDALRKSLSETTGEIEKILAAKSKLDSNMNTQLLM
EQLVLEIQGR

Sequences:

>Translated_330_residues
MGLQDELTVMQPVVMKIFSKSVRENRLSHGYLFEGARGTGKKRTALWLAQSLFCLESTETELACGKCANCTRIASHNHPD
VHLLEPDGASIKIDQVRALKQELSKRGMESDQKVVIIYDAEKMTVQSANSLLKFIEEPEGGLLLLFLTTNPGQILPTIQS
RLQPVTFKSLTFDSLLASLTAAGISEQKARIYASITGSVEEAKAFEESEWFSEARNVVIKLYEGIHHQGTSPLIIIQESW
MPLFKEKDKMALGLELLLLLYRDRLHLTLDENYEPICTAQKEMLGQDALRKSLSETTGEIEKILAAKSKLDSNMNTQLLM
EQLVLEIQGR
>Mature_329_residues
GLQDELTVMQPVVMKIFSKSVRENRLSHGYLFEGARGTGKKRTALWLAQSLFCLESTETELACGKCANCTRIASHNHPDV
HLLEPDGASIKIDQVRALKQELSKRGMESDQKVVIIYDAEKMTVQSANSLLKFIEEPEGGLLLLFLTTNPGQILPTIQSR
LQPVTFKSLTFDSLLASLTAAGISEQKARIYASITGSVEEAKAFEESEWFSEARNVVIKLYEGIHHQGTSPLIIIQESWM
PLFKEKDKMALGLELLLLLYRDRLHLTLDENYEPICTAQKEMLGQDALRKSLSETTGEIEKILAAKSKLDSNMNTQLLME
QLVLEIQGR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=168, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36968; Mature: 36837

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLQDELTVMQPVVMKIFSKSVRENRLSHGYLFEGARGTGKKRTALWLAQSLFCLESTET
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
ELACGKCANCTRIASHNHPDVHLLEPDGASIKIDQVRALKQELSKRGMESDQKVVIIYDA
HHHCCCCCCCHHHHCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEECC
EKMTVQSANSLLKFIEEPEGGLLLLFLTTNPGQILPTIQSRLQPVTFKSLTFDSLLASLT
CHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHH
AAGISEQKARIYASITGSVEEAKAFEESEWFSEARNVVIKLYEGIHHQGTSPLIIIQESW
HHCCCHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
MPLFKEKDKMALGLELLLLLYRDRLHLTLDENYEPICTAQKEMLGQDALRKSLSETTGEI
CHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EKILAAKSKLDSNMNTQLLMEQLVLEIQGR
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GLQDELTVMQPVVMKIFSKSVRENRLSHGYLFEGARGTGKKRTALWLAQSLFCLESTET
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
ELACGKCANCTRIASHNHPDVHLLEPDGASIKIDQVRALKQELSKRGMESDQKVVIIYDA
HHHCCCCCCCHHHHCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEECC
EKMTVQSANSLLKFIEEPEGGLLLLFLTTNPGQILPTIQSRLQPVTFKSLTFDSLLASLT
CHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHH
AAGISEQKARIYASITGSVEEAKAFEESEWFSEARNVVIKLYEGIHHQGTSPLIIIQESW
HHCCCHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
MPLFKEKDKMALGLELLLLLYRDRLHLTLDENYEPICTAQKEMLGQDALRKSLSETTGEI
CHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EKILAAKSKLDSNMNTQLLMEQLVLEIQGR
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]