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The map label for this gene is ykoW [H]

Identifier: 226222770

GI number: 226222770

Start: 136685

End: 137434

Strand: Direct

Name: ykoW [H]

Synonym: Lm4b_00135

Alternate gene names: 226222770

Gene position: 136685-137434 (Clockwise)

Preceding gene: 226222768

Following gene: 226222772

Centisome position: 4.69

GC content: 35.07

Gene sequence:

>750_bases
ATGGATATCTCAAGTACGGAAATATGGGATGCAATTAGGAGAAATAGTTATTTGCTATATTATCAACCAAAAGTAGACGC
GAAAACGAATAAAATTATTGGCTTTGAAGGTTTGATTCGATTAAAAACGGCAACAACCATTTTGGCTCCAATTGATTTCT
TTGATGATATTGTCCTTTTAAATGCAACGCGGGAAATGCAAGATTTTGTTGCTGAAACAGCGATAAAACAGATTAATCAG
CTAGGAGGACGTTTTTCAATTTCGATTAATATTCCGGCACATTATGTAGCGAGCAGTACGTATATGACTTTTTTACATGA
TTATGTGAAAGAACATTTAAAGTATCCGGAATGCTTAGAAATAGAGATTATCGAACGAGGCGAAATAACGGAACTAGCTA
TAGCGGATAAAAACTTACGAAAAATAAAAGACCTTGGTGTCAAAGTAAGTATGGATGATTTTGGGAAAGGTTATAGTTCG
CTTGCGTATTTGCGCAGCTTACCGATTGATATTGTGAAAACAGATATGTCCTTTATCGCGCTTTTAAAAACAGATCGAAA
ACAACAAATTATTATTCGAGCGATTGTCAATTTGTGTCATGATTTAGGCGGGAAAGTAGTTACAGAAGGCGTTGAAGACA
TGGAGCAAGTCGAAAAATTACGGGAAATGAAAGTAGACTATTTCCAGGGCTATTATTTTAGTCGACCACTACCGATGGAA
GATATTAAAGAAAAATATTCCTTTGTGTAA

Upstream 100 bases:

>100_bases
CAATAGGAAATATGGATTTTGTGTAAATGTAGTGATAATATTTGGCTATATTTTCATTTCGATGGAAAAAAGGGTAAACT
AGAGAAAAAGGAGGGATAAG

Downstream 100 bases:

>100_bases
GATGTATAAATAAAAAATTGGTTGGTGTGAGAATGGTAATTTACCAAATTCACACCAACCAATTTTATTTTATTTGATTA
AATTATTTGAAGCATCTTTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MDISSTEIWDAIRRNSYLLYYQPKVDAKTNKIIGFEGLIRLKTATTILAPIDFFDDIVLLNATREMQDFVAETAIKQINQ
LGGRFSISINIPAHYVASSTYMTFLHDYVKEHLKYPECLEIEIIERGEITELAIADKNLRKIKDLGVKVSMDDFGKGYSS
LAYLRSLPIDIVKTDMSFIALLKTDRKQQIIIRAIVNLCHDLGGKVVTEGVEDMEQVEKLREMKVDYFQGYYFSRPLPME
DIKEKYSFV

Sequences:

>Translated_249_residues
MDISSTEIWDAIRRNSYLLYYQPKVDAKTNKIIGFEGLIRLKTATTILAPIDFFDDIVLLNATREMQDFVAETAIKQINQ
LGGRFSISINIPAHYVASSTYMTFLHDYVKEHLKYPECLEIEIIERGEITELAIADKNLRKIKDLGVKVSMDDFGKGYSS
LAYLRSLPIDIVKTDMSFIALLKTDRKQQIIIRAIVNLCHDLGGKVVTEGVEDMEQVEKLREMKVDYFQGYYFSRPLPME
DIKEKYSFV
>Mature_249_residues
MDISSTEIWDAIRRNSYLLYYQPKVDAKTNKIIGFEGLIRLKTATTILAPIDFFDDIVLLNATREMQDFVAETAIKQINQ
LGGRFSISINIPAHYVASSTYMTFLHDYVKEHLKYPECLEIEIIERGEITELAIADKNLRKIKDLGVKVSMDDFGKGYSS
LAYLRSLPIDIVKTDMSFIALLKTDRKQQIIIRAIVNLCHDLGGKVVTEGVEDMEQVEKLREMKVDYFQGYYFSRPLPME
DIKEKYSFV

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790496, Length=246, Percent_Identity=31.7073170731707, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI87081921, Length=247, Percent_Identity=31.5789473684211, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788502, Length=252, Percent_Identity=28.968253968254, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI226510982, Length=248, Percent_Identity=27.4193548387097, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI87081743, Length=240, Percent_Identity=30, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787541, Length=238, Percent_Identity=27.3109243697479, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87081845, Length=241, Percent_Identity=28.2157676348548, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI87081980, Length=252, Percent_Identity=28.968253968254, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1786507, Length=237, Percent_Identity=26.5822784810127, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI87082096, Length=237, Percent_Identity=27.8481012658228, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788849, Length=123, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787055, Length=237, Percent_Identity=29.535864978903, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787410, Length=216, Percent_Identity=24.537037037037, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 28673; Mature: 28673

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISSTEIWDAIRRNSYLLYYQPKVDAKTNKIIGFEGLIRLKTATTILAPIDFFDDIVLL
CCCCHHHHHHHHHHCCEEEEECCCCCCCCCEEECHHHEEEEEHHHHHHHHHHHHHCEEEE
NATREMQDFVAETAIKQINQLGGRFSISINIPAHYVASSTYMTFLHDYVKEHLKYPECLE
ECHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IEIIERGEITELAIADKNLRKIKDLGVKVSMDDFGKGYSSLAYLRSLPIDIVKTDMSFIA
EEEECCCCEEEEEECCHHHHHHHHCCCEEEHHHHCCCHHHHHHHHHCCHHHHHHHHHHHH
LLKTDRKQQIIIRAIVNLCHDLGGKVVTEGVEDMEQVEKLREMKVDYFQGYYFSRPLPME
HHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHH
DIKEKYSFV
HHHHHHCCC
>Mature Secondary Structure
MDISSTEIWDAIRRNSYLLYYQPKVDAKTNKIIGFEGLIRLKTATTILAPIDFFDDIVLL
CCCCHHHHHHHHHHCCEEEEECCCCCCCCCEEECHHHEEEEEHHHHHHHHHHHHHCEEEE
NATREMQDFVAETAIKQINQLGGRFSISINIPAHYVASSTYMTFLHDYVKEHLKYPECLE
ECHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IEIIERGEITELAIADKNLRKIKDLGVKVSMDDFGKGYSSLAYLRSLPIDIVKTDMSFIA
EEEECCCCEEEEEECCHHHHHHHHCCCEEEHHHHCCCHHHHHHHHHCCHHHHHHHHHHHH
LLKTDRKQQIIIRAIVNLCHDLGGKVVTEGVEDMEQVEKLREMKVDYFQGYYFSRPLPME
HHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHH
DIKEKYSFV
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]