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The map label for this gene is pbpF [H]

Identifier: 225861830

GI number: 225861830

Start: 1868147

End: 1870342

Strand: Reverse

Name: pbpF [H]

Synonym: SPT_2006

Alternate gene names: 225861830

Gene position: 1870342-1868147 (Counterclockwise)

Preceding gene: 225861832

Following gene: 225861829

Centisome position: 88.55

GC content: 41.39

Gene sequence:

>2196_bases
ATGAAATTAGATAAATTATTTGAGAAATTTCTTTCTCTTTTTAAAAAAGAAACAAGTGAACTAGAGGACTCTGATTCTAC
TATCTTACGTCGCTCTCGTAGTGATCGAAAAAAATTAGCCCAAGTAGGTCCGATTCGAAAATTCTGGCGTCGTTATCATC
TAACAAAGATTATCCTTATACTAGGTTTGAGTGCAGGATTGCTAGTTGGAATCTATTTGTTTGCTGTAGCCAAGTCGACC
AATGTCAATGATTTGCAAAATGCCTTGAAAACTCGGACTCTTATTTTTGACCGTGAAGAAAAAGAGGCTGGTGCCTTGTC
TGGTCAAAAGGGAACCTATGTTGAGCTGACTGACATCAGTAAAAACTTGCAGAATGCTGTTATTGCGACAGAAGACCGTT
CTTTCTATAAAAATGACGGGATTAACTATGGCCGTTTCTTCTTGGCTATTGTCACTGCTGGACGTTCAGGTGGTGGCTCT
ACCATTACCCAACAGCTGGCTAAAAACGCCTATTTATCGCAGGATCAAACTGTTGAGAGAAAAGCGAAAGAATTTTTCCT
TGCCTTAGAATTAAGCAAAAAATATAGTAAGGAGCAAATTCTAACCATGTACCTTAACAACGCTTATTTTGGAAATGGTG
TGTGGGGTGTAGAAGATGCGAGTAAGAAATACTTTGGAGTTTCTGCATCAGAAGTGAGTCTGGATCAAGCTGCGACTCTG
GCAGGGATGCTCAAGGGGCCGGAACTGTATAATCCCTTGAATTCTGTAGAAGATTCTACTAATCGGCGCGATACTGTCTT
GCAGAATATGGTTGCAGCAGGATATATTGATAAAAACCAAGAAACCGAAGCTGCTGAAGTTGATATGACTTCGCAATTGC
ACGATAAGTATGAAGGAAAAATCTCAGATTACCGTTACCCCTCTTATTTTGATGCGGTGGTTAATGAAGCTGTTTCCAAG
TATAATCTAACAGAGGAAGAGATTGTCAATAATGGCTACCGCATTTACACAGAGCTGGACCAAAACTACCAAGCAAATAT
GCAGATTGTTTATGAAAACATATCGCTATTTCCGAGGGCAGAGGATGGAACGTTTGCTCAATCAGGAAGTGTAGCTCTCG
AACCGAAAACAGGGGGAGTTCGTGGAGTTGTCGGTCAAGTTGCTGACAATGATAAAACTGGATTCCGGAATTTCAACTAT
GCAACCCAATCAAAGCGTAGTCCTGGTTCTACAATTAAGCCTTTAGTTGTTTATACGCCAGCAGTTGAAGCAGGCTGGGC
TTTGAATAAGCAGTTGGATAACCATACCATGCAGTATGACAGCTATAAGGTTGATAACTATGCAGGGATCAAAACGAGTC
GAGAAGTTCCTATGTATCAAGCCTTGGCAGAATCGCTTAATCTACCTGCTGTTGCCACTGTTAATGATTTGGGTGTCGAC
AAGGCTTTTGAGGCAGGCGAAAAATTCGGACTCAACATGGAAAAGGTTGACCGTGTTCTTGGTGTCGCCTTGGGAAGCGG
TGTTGAAACCAACCCTCTTCAAATGGCTCAAGCATACGCCGCCTTTGCAAATGAAGGTTTAATGCCTGAAGCTCATTTTA
TTAGTAGAATTGAAAATGCTAGTGGACAAGTTATTGCGAGTCATAAAAATTCACAAAAACGGGTGATTGATAAGTCTGTA
GCTGACAAGATGACCAGTATGATGTTGGGGACTTTCACCAACGGTACCGGTATTAGTTCATCGCCTGCAGACTATATTAT
GGCAGGGAAAACTGGAACAACTGAAGCAGTTTTTAATCCGGAGTACACAAGTGACCAGTGGGTAATTGGTTATACTCCGG
ATGTAGTGATTAGCCACTGGCTTGGCTTTCCGACCACTGATGAAAACCACTATCTAGCTGGCTCTACTTCAAACGGTGCA
GCTCATGTCTTTAGAAACATTGCCAATACCATTTTACCTTATACGTCAGGAAGTACCTTTACGGTTGAAAATGCTTATAA
GCAAAATGGAATTGCACCAGCCAATACAAAAAGACAAGTACAAACCAATGATAATAGCCAGACAGATGATAATTTGTCTG
ATATTCGAGGGCGTGCGCAAAGTCTAGTAGATGAGGCTAGCCGGGCTATCTCAGATGCGAAGATTAAGGAAAAGGCTCAA
ACAATATGGGATTCGATAGTCAATCTATTTCGCTAA

Upstream 100 bases:

>100_bases
AATGTGAATTGCATACAGATATTGTAACAAAAAAAGCCCTATTTGGATAGGAAATAGCTAAATTCTTGTCTTCCTATGAT
GAAGATGATAAAATAAACGC

Downstream 100 bases:

>100_bases
GATGCTTGTCAAAGCCTAGCTTTCTTGTTATAATGGATAAGATGGAGGCGTTATGGCACTAAAAAAAGCAAGCCTAGCTT
GTGCGGTTTGTGGTTCGAGA

Product: penicillin-binding protein 2A

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 731; Mature: 731

Protein sequence:

>731_residues
MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGPIRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKST
NVNDLQNALKTRTLIFDREEKEAGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVEDASKKYFGVSASEVSLDQAATL
AGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYIDKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSK
YNLTEEEIVNNGYRIYTELDQNYQANMQIVYENISLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDKTGFRNFNY
ATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGIKTSREVPMYQALAESLNLPAVATVNDLGVD
KAFEAGEKFGLNMEKVDRVLGVALGSGVETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSV
ADKMTSMMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFPTTDENHYLAGSTSNGA
AHVFRNIANTILPYTSGSTFTVENAYKQNGIAPANTKRQVQTNDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQ
TIWDSIVNLFR

Sequences:

>Translated_731_residues
MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGPIRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKST
NVNDLQNALKTRTLIFDREEKEAGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVEDASKKYFGVSASEVSLDQAATL
AGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYIDKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSK
YNLTEEEIVNNGYRIYTELDQNYQANMQIVYENISLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDKTGFRNFNY
ATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGIKTSREVPMYQALAESLNLPAVATVNDLGVD
KAFEAGEKFGLNMEKVDRVLGVALGSGVETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSV
ADKMTSMMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFPTTDENHYLAGSTSNGA
AHVFRNIANTILPYTSGSTFTVENAYKQNGIAPANTKRQVQTNDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQ
TIWDSIVNLFR
>Mature_731_residues
MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGPIRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKST
NVNDLQNALKTRTLIFDREEKEAGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVEDASKKYFGVSASEVSLDQAATL
AGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYIDKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSK
YNLTEEEIVNNGYRIYTELDQNYQANMQIVYENISLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDKTGFRNFNY
ATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGIKTSREVPMYQALAESLNLPAVATVNDLGVD
KAFEAGEKFGLNMEKVDRVLGVALGSGVETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSV
ADKMTSMMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFPTTDENHYLAGSTSNGA
AHVFRNIANTILPYTSGSTFTVENAYKQNGIAPANTKRQVQTNDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQ
TIWDSIVNLFR

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=563, Percent_Identity=28.2415630550622, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI87082258, Length=310, Percent_Identity=31.2903225806452, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1788867, Length=535, Percent_Identity=27.6635514018692, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1789601, Length=166, Percent_Identity=35.5421686746988, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 80799; Mature: 80799

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGPIRKFWRRYHLTKIILI
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKEAGALSGQKGTYVELTDIS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCHHHHCCCCCCCCCEEEEHHHH
KNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGSTITQQLAKNAYLSQDQTVER
HHHHHCEEEECCCCHHHCCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHH
KAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVEDASKKYFGVSASEVSLDQAATL
HHHHHHHHHHHHHHCCHHHHHHHEECCCEECCCCCCCCCCCCHHCCCCHHHHHHHHHHHH
AGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYIDKNQETEAAEVDMTSQLHDKYEGK
HHHHCCCHHHCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
ISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYRIYTELDQNYQANMQIVYENISLFPRA
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCCCCEEEEEECCEECCCC
EDGTFAQSGSVALEPKTGGVRGVVGQVADNDKTGFRNFNYATQSKRSPGSTIKPLVVYTP
CCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEECC
AVEAGWALNKQLDNHTMQYDSYKVDNYAGIKTSREVPMYQALAESLNLPAVATVNDLGVD
CCCCCCHHHHHHCCCCEECCCEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHCCCH
KAFEAGEKFGLNMEKVDRVLGVALGSGVETNPLQMAQAYAAFANEGLMPEAHFISRIENA
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
SGQVIASHKNSQKRVIDKSVADKMTSMMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNP
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCEEECC
EYTSDQWVIGYTPDVVISHWLGFPTTDENHYLAGSTSNGAAHVFRNIANTILPYTSGSTF
CCCCCCEEEECCHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCEE
TVENAYKQNGIAPANTKRQVQTNDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQ
EECCHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIWDSIVNLFR
HHHHHHHHHHC
>Mature Secondary Structure
MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGPIRKFWRRYHLTKIILI
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKEAGALSGQKGTYVELTDIS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCHHHHCCCCCCCCCEEEEHHHH
KNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGSTITQQLAKNAYLSQDQTVER
HHHHHCEEEECCCCHHHCCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHH
KAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVEDASKKYFGVSASEVSLDQAATL
HHHHHHHHHHHHHHCCHHHHHHHEECCCEECCCCCCCCCCCCHHCCCCHHHHHHHHHHHH
AGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYIDKNQETEAAEVDMTSQLHDKYEGK
HHHHCCCHHHCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
ISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYRIYTELDQNYQANMQIVYENISLFPRA
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCCCCEEEEEECCEECCCC
EDGTFAQSGSVALEPKTGGVRGVVGQVADNDKTGFRNFNYATQSKRSPGSTIKPLVVYTP
CCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEECC
AVEAGWALNKQLDNHTMQYDSYKVDNYAGIKTSREVPMYQALAESLNLPAVATVNDLGVD
CCCCCCHHHHHHCCCCEECCCEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHCCCH
KAFEAGEKFGLNMEKVDRVLGVALGSGVETNPLQMAQAYAAFANEGLMPEAHFISRIENA
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
SGQVIASHKNSQKRVIDKSVADKMTSMMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNP
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCEEECC
EYTSDQWVIGYTPDVVISHWLGFPTTDENHYLAGSTSNGAAHVFRNIANTILPYTSGSTF
CCCCCCEEEECCHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCEE
TVENAYKQNGIAPANTKRQVQTNDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQ
EECCHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIWDSIVNLFR
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]