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The map label for this gene is trpE [H]

Identifier: 225861619

GI number: 225861619

Start: 1652964

End: 1654325

Strand: Reverse

Name: trpE [H]

Synonym: SPT_1740

Alternate gene names: 225861619

Gene position: 1654325-1652964 (Counterclockwise)

Preceding gene: 225861620

Following gene: 225861618

Centisome position: 78.32

GC content: 44.57

Gene sequence:

>1362_bases
ATGGAACGAATCATTCATGGAGATGTCTTATCACCAATCTTGGCTTATATGCGCTTAAAGGGGCAACACAAGGTTATCTT
AGAGAGTATTCCGAGAGACAAGGAAACAGCTCGTTTTTCTATCCTAGCCTATAATCCAATTTTTGAGATTAAGTTTGAAA
ATGGAGTTCTCTATCAAAATGGTCAAGTGATTGATCGGGATCCCTTGGATTTCCTTTATGAAGTGATTCATAAGAGTCAG
CACCACTCAGAGCTACCTTTTGGTGGTGGAGCCGTTGGTTTTGTGGGTTACGATATGATTTCACTTTATGAAGAAATTGG
TCAAATCCCTGAGGATACAATTGGGACGCCAGACATGCATTTCTTTGTCTATGAGAGCTATATGGTCTTTGACCACAAGA
AGGAGAAGATACATGTCATAGAGGATGCACTTTATAGTGAGCGCAGTCAAGAGGCCTTGGAAAAATCCTTGAACCAAGTG
CTTGAGGAATTACGCATTCCTGCTCCAAATGAATTTGAAGACTTGGATCTATCTCCGTTAGACTTCAAACCTCATATCGC
TCCTCAGAAGTTTGAGGAAATGGTGGAAACAGCTCGTGACTTGATTCGTAATGGTGATATGTTCCAATGCGTACTCAGCC
AGCGCTTTTCAGCAGAGGTTACTGGAAATCCATTTGACTTCTACAGAAATCTCCGCGTGACCAATCCATCTAATTACCTT
TATTTCTATGACTTTGGGGATTATCAAATCATCGGTGCCAGTCCAGAAAGTTTGGTTTCTGTCAAAAATGGCATCGTGAC
AACCAATCCGATTGCAGGGACGCGACCAAGAGGGGCTACGGATGAAGAAGACAAGACCTTGGCGACTGACCTCCTCTCTG
ATGAGAAGGAAACAGCAGAACATCGAATGTTGGTAGACTTGGGGCGTAACGATATTGGCCGCATCTCTGAAACGGCCAGT
GTCCAAGTTACTAAGTATATGGAGGTGGAGCTCTTCCGCTACGTCATGCATTTGACCAGTGTGGTCAAGGGACGTTTGCT
TGCAGAACTCACTGCCATGGATGCCTTGAAAGCAACGCTTCCTGCTGGAACCGTTTCTGGAGCACCAAAGATTCGGGCGA
TGAGACGCATCTATGAACTGGAAACGGAAAAACGGGGCGTATATGCAGGAGCAATCGGCTACTTGTCTGCGACGGGTGAT
ATGGATTTGGCCATCGCCATCCGAACCATGATTCTCAAAAATCAAAGAGCCTATGTGCAGGCTGGGGCAGGGATTGTCTA
CGACTCTATCGCCCAAAACGAATACCAAGAAACCATTAACAAAGCAAAATCTATGACTAGAATTGGAGAACTAAGACCAT
GA

Upstream 100 bases:

>100_bases
GAGGTGGCACCGCGAATTTCGTCCTCACGCAAGTTATTTTGCGTGGGGATTTTTCATACAGTCGCTACGAACTAGAATTA
GAACTGAAAGGAAAATTACA

Downstream 100 bases:

>100_bases
TTTTATTGATTGACAACTATGATTCTTTTACCTATAACTTGGCCCAATACATTGGGAATTTTGCAGAAGTTCAGGTTCTG
AGAAATGATGATTCCAAGCT

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAYNPIFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQ
HHSELPFGGGAVGFVGYDMISLYEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
LEELRIPAPNEFEDLDLSPLDFKPHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSAEVTGNPFDFYRNLRVTNPSNYL
YFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRPRGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETAS
VQVTKYMEVELFRYVMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAIGYLSATGD
MDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGELRP

Sequences:

>Translated_453_residues
MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAYNPIFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQ
HHSELPFGGGAVGFVGYDMISLYEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
LEELRIPAPNEFEDLDLSPLDFKPHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSAEVTGNPFDFYRNLRVTNPSNYL
YFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRPRGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETAS
VQVTKYMEVELFRYVMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAIGYLSATGD
MDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGELRP
>Mature_453_residues
MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAYNPIFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQ
HHSELPFGGGAVGFVGYDMISLYEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
LEELRIPAPNEFEDLDLSPLDFKPHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSAEVTGNPFDFYRNLRVTNPSNYL
YFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRPRGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETAS
VQVTKYMEVELFRYVMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAIGYLSATGD
MDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGELRP

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=397, Percent_Identity=36.7758186397985, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1788114, Length=431, Percent_Identity=33.4106728538283, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1786809, Length=262, Percent_Identity=26.7175572519084, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082077, Length=265, Percent_Identity=26.7924528301887, Blast_Score=75, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=454, Percent_Identity=38.7665198237885, Blast_Score=283, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6324361, Length=407, Percent_Identity=27.2727272727273, Blast_Score=123, Evalue=5e-29,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 51249; Mature: 51249

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAYNPIFEIKFENGVLYQN
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEEEECCEEEEEECCCEEEEC
GQVIDRDPLDFLYEVIHKSQHHSELPFGGGAVGFVGYDMISLYEEIGQIPEDTIGTPDMH
CEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
FFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQVLEELRIPAPNEFEDLDLSPL
EEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DFKPHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSAEVTGNPFDFYRNLRVTNPSNYL
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHCCCEEECCCCEE
YFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRPRGATDEEDKTLATDLLSDEKETAE
EEEECCCEEEECCCHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
HRMLVDLGRNDIGRISETASVQVTKYMEVELFRYVMHLTSVVKGRLLAELTAMDALKATL
HHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PAGTVSGAPKIRAMRRIYELETEKRGVYAGAIGYLSATGDMDLAIAIRTMILKNQRAYVQ
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEHHEEEECCCCCHHHHHHHHHHHHCCHHEEE
AGAGIVYDSIAQNEYQETINKAKSMTRIGELRP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAYNPIFEIKFENGVLYQN
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEEEECCEEEEEECCCEEEEC
GQVIDRDPLDFLYEVIHKSQHHSELPFGGGAVGFVGYDMISLYEEIGQIPEDTIGTPDMH
CEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
FFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQVLEELRIPAPNEFEDLDLSPL
EEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DFKPHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSAEVTGNPFDFYRNLRVTNPSNYL
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHCCCEEECCCCEE
YFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRPRGATDEEDKTLATDLLSDEKETAE
EEEECCCEEEECCCHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
HRMLVDLGRNDIGRISETASVQVTKYMEVELFRYVMHLTSVVKGRLLAELTAMDALKATL
HHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PAGTVSGAPKIRAMRRIYELETEKRGVYAGAIGYLSATGDMDLAIAIRTMILKNQRAYVQ
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEHHEEEECCCCCHHHHHHHHHHHHCCHHEEE
AGAGIVYDSIAQNEYQETINKAKSMTRIGELRP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400208; 11337471 [H]