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The map label for this gene is spoT [H]

Identifier: 225861474

GI number: 225861474

Start: 1502082

End: 1504304

Strand: Reverse

Name: spoT [H]

Synonym: SPT_1585

Alternate gene names: 225861474

Gene position: 1504304-1502082 (Counterclockwise)

Preceding gene: 225861476

Following gene: 225861473

Centisome position: 71.22

GC content: 45.48

Gene sequence:

>2223_bases
ATGCCGAAAGAAGTGAATTTAACAGGCGAAGAAGTTGTCGCTTTAACCAAAGAATATTTAACGGAAGAGGATGTTTATTT
TGTCCATAAGGCCTTGGTCTATGCTGTTGAATGCCACAGTGGTCAATATCGCAAATCAGGCGAGCCTTATATCATTCACC
CTATCCAAGTGGCAGGTATTTTAGCTAAGCTAAAGCTGGATGCTGTAACAGTAGCTTGTGGATTCTTGCATGATGTGGTG
GAAGATACAGATGCGACCTTGGACGATTTGGAAAGAGAGTTTGGTCCTGATGTGCGGATGATTGTTGACGGAGTTACCAA
GCTTGGCAAGGTCGAGTACAAATCGATCGAGGAGCAATTAGCGGAAAATCATCGCAAGATGCTCATGGCCATGTCTGAGG
ACATCCGCGTTATTTTGGTCAAACTGTCTGACCGCTTGCACAATATGCGGACCCTGAAACATCTTCGAAAAGACAAGCAG
GAGCGTATTTCCAAAGAAACCATGGAAATCTATGCCCCGCTTGCCCATCGTTTGGGGATTTCCAGTGTCAAATGGGAATT
AGAAGACTTGTCTTTCCGTTATCTCAATCCAACGGAGTTTTACAAGATTACCCATATGATGAAGGAAAAGCGCAGGGAGC
GTGAGGCCTTGGTGGATGAGGTAGTCACAAAATTAGAGGAGTATACGACAGAACGTCACTTGAAAGGGAAGATTTATGGT
CGTCCCAAGCATATTTACTCAATTTTCCGCAAAATGCAGGACAAGAGAAAACGGTTTGAGGAAATCTATGATCTGATTGC
TATTCGTTGTATTTTAGATACCCAAAGTGATGTTTATGCCATGCTTGGTTACGTGCATGAATTTTGGAAACCGATGCCAG
GTCGCTTCAAAGACTATATCGCCAACCGCAAGGCCAATGGTTATCAGTCTATCCATACGACTGTTTATGGACCAAAAGGG
CCGATTGAATTCCAGATTCGAACCAAGGAAATGCACGAGGTGGCTGAGTACGGGGTTGCGGCTCACTGGGCTTATAAGAA
AGGTATAAAGGGGCAAGTTAACAGCAAGGAATCAGCTATTGGAATGAACTGGATCAAGGAGATGATAGAGCTCCAAGACC
AGGCTGATGATGCTAAGGAATTTGTGGACTCTGTTAAGGAAAACTATCTGGCTGAGGAGATTTACGTTTTTACCCCAGAT
GGAGCTGTCCGTTCTCTTCCCAAAGATTCAGGACCGATTGATTTTGCCTACGAAATCCATACCAAGGTCGGTGAAAAAGC
AACTGGTGCCAAGGTCAATGGCCGCATGGTTCCACTGACAACCAAGTTAAAGACAGGGGATCAGGTTGAAATTATCGCCA
ACCCGAACTCCTTTGGACCTAGCCGTGACTGGCTCAATATGGTCAAGACTAGCAAGGCGCGCAATAAGATTCGCCAGTTC
TTTAAAAACCAAGATAAGGAATTGTCTGTCAACAAGGGTCGTGAGATGCTGATGGCTCAGTTCCAAGAAAATGGCTATGT
GGCAAATAAATTTATGGACAAGCGCCACATGGATCAAGTTCTGCAAAAGACCAGTTACAAGACAGAAGACTCCCTCTTTG
CGGCCATTGGTTTTGGGGAAATCGGTGCGATTACCGTCTTTAACCGTCTGACTGAAAAAGAACGCCGTGAGGAAGAGCGT
GCCAAGGCCAAGGCTGAGGCAGAGGAGCTTGTCAAAGGTGGCGAGGTCAAGGTTGAAAATAAAGAAACTCTCAAGGTCAA
GCATGAGGGGGGAGTGGTTATTGAAGGTGCTTCTGGTCTCCTAGTGCGGATTGCTAAGTGTTGTAACCCCGTGCCTGGTG
ACGATATTGTTGGCTACATTACCAAGGGTCGTGGTGTGGCTATTCACCGTGTGGACTGTATGAACCTGCGTGCCCAAGAA
AACTACGAGCAACGTCTCCTTGATGTGGAATGGGAAGACCAGTACTCTAGCTCAAATAAGGAGTATATGGCCCATATCGA
TATCTACGGTCTCAACCGTACAGGACTGTTGAACGATGTACTGCAAGTTCTTTCAAATACAACCAAGAATATTTCAACGG
TCAATGCCCAACCAACCAAGGATATGAAGTTTGCTAATATCCATGTGTCCTTCGGTATTGCCAACCTCTCTACACTGACC
ACGGTTGTCGATAAAATTAAGAGTGTGCCAGAAGTTTACTCTGTCAAACGGACCAACGGCTAG

Upstream 100 bases:

>100_bases
TATCAAGCAATTTTGAATTATTTTTTTCTTATTCTGTTCCAATCTAGTTTTCATATTCTTAAAAAAGTGATAAAATGGTA
GGAAGAATTGGAGAGTAGAG

Downstream 100 bases:

>100_bases
TTGTCCTAGCTCTTACTAGAAAGGCTATTATGAAAATCATTATCCAACGGGTTAAAAAAGCCCAAGTGAGTATAGAAGGC
CAGATTCAGGGAAAAATCAA

Product: guanosine-3,5-bis(diphosphate) 3-pyrophosphohydrolase

Products: NA

Alternate protein names: GTP pyrophosphokinase; (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; Stringent response-like protein; ppGpp synthase I; Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase; Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 740; Mature: 739

Protein sequence:

>740_residues
MPKEVNLTGEEVVALTKEYLTEEDVYFVHKALVYAVECHSGQYRKSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVV
EDTDATLDDLEREFGPDVRMIVDGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALVDEVVTKLEEYTTERHLKGKIYG
RPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQSDVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKG
PIEFQIRTKEMHEVAEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMIELQDQADDAKEFVDSVKENYLAEEIYVFTPD
GAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVEIIANPNSFGPSRDWLNMVKTSKARNKIRQF
FKNQDKELSVNKGREMLMAQFQENGYVANKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEER
AKAKAEAEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGRGVAIHRVDCMNLRAQE
NYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQVLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLT
TVVDKIKSVPEVYSVKRTNG

Sequences:

>Translated_740_residues
MPKEVNLTGEEVVALTKEYLTEEDVYFVHKALVYAVECHSGQYRKSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVV
EDTDATLDDLEREFGPDVRMIVDGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALVDEVVTKLEEYTTERHLKGKIYG
RPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQSDVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKG
PIEFQIRTKEMHEVAEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMIELQDQADDAKEFVDSVKENYLAEEIYVFTPD
GAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVEIIANPNSFGPSRDWLNMVKTSKARNKIRQF
FKNQDKELSVNKGREMLMAQFQENGYVANKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEER
AKAKAEAEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGRGVAIHRVDCMNLRAQE
NYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQVLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLT
TVVDKIKSVPEVYSVKRTNG
>Mature_739_residues
PKEVNLTGEEVVALTKEYLTEEDVYFVHKALVYAVECHSGQYRKSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVE
DTDATLDDLEREFGPDVRMIVDGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQE
RISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALVDEVVTKLEEYTTERHLKGKIYGR
PKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQSDVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGP
IEFQIRTKEMHEVAEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMIELQDQADDAKEFVDSVKENYLAEEIYVFTPDG
AVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVEIIANPNSFGPSRDWLNMVKTSKARNKIRQFF
KNQDKELSVNKGREMLMAQFQENGYVANKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERA
KAKAEAEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGRGVAIHRVDCMNLRAQEN
YEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQVLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTT
VVDKIKSVPEVYSVKRTNG

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes both the formation of pppGpp wh

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=126, Percent_Identity=40.4761904761905, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1790082, Length=729, Percent_Identity=39.0946502057613, Blast_Score=549, Evalue=1e-157,
Organism=Escherichia coli, GI1789147, Length=700, Percent_Identity=37.4285714285714, Blast_Score=487, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI25143118, Length=136, Percent_Identity=39.7058823529412, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24650996, Length=131, Percent_Identity=39.6946564885496, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5; =3.1.7.2 [H]

Molecular weight: Translated: 84655; Mature: 84524

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKEVNLTGEEVVALTKEYLTEEDVYFVHKALVYAVECHSGQYRKSGEPYIIHPIQVAGI
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCCCCCEEEEHHHHHHH
LAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIVDGVTKLGKVEYKSIEEQL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHH
AENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQERISKETMEIYAPLAHRLGI
HHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALVDEVVTKLEEYTTERHLKGKIYG
CHHEEEHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQSDVYAMLGYVHEFWKPMPGRFKDYI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHH
ANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEVAEYGVAAHWAYKKGIKGQVNSKESAI
HCCCCCCCCCEEEEEECCCCCEEEEEEHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHH
GMNWIKEMIELQDQADDAKEFVDSVKENYLAEEIYVFTPDGAVRSLPKDSGPIDFAYEIH
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEECCCCHHHCCCCCCCCCEEEEEEH
TKVGEKATGAKVNGRMVPLTTKLKTGDQVEIIANPNSFGPSRDWLNMVKTSKARNKIRQF
HHCCCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
FKNQDKELSVNKGREMLMAQFQENGYVANKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGE
HCCCCCCCCHHCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
IGAITVFNRLTEKERREEERAKAKAEAEELVKGGEVKVENKETLKVKHEGGVVIEGASGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCEEEECCCHH
LVRIAKCCNPVPGDDIVGYITKGRGVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNK
HHHHHHHCCCCCCCCCEEEEECCCCEEEEEEHHHCCCHHCCHHHHHCCCCCCCCCCCCCC
EYMAHIDIYGLNRTGLLNDVLQVLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLT
CEEEEEEEEECCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCEEEEEEEEECHHHHHHHH
TVVDKIKSVPEVYSVKRTNG
HHHHHHHCCHHHHHHCCCCC
>Mature Secondary Structure 
PKEVNLTGEEVVALTKEYLTEEDVYFVHKALVYAVECHSGQYRKSGEPYIIHPIQVAGI
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCCCCCEEEEHHHHHHH
LAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIVDGVTKLGKVEYKSIEEQL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHH
AENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQERISKETMEIYAPLAHRLGI
HHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALVDEVVTKLEEYTTERHLKGKIYG
CHHEEEHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQSDVYAMLGYVHEFWKPMPGRFKDYI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHH
ANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEVAEYGVAAHWAYKKGIKGQVNSKESAI
HCCCCCCCCCEEEEEECCCCCEEEEEEHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHH
GMNWIKEMIELQDQADDAKEFVDSVKENYLAEEIYVFTPDGAVRSLPKDSGPIDFAYEIH
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEECCCCHHHCCCCCCCCCEEEEEEH
TKVGEKATGAKVNGRMVPLTTKLKTGDQVEIIANPNSFGPSRDWLNMVKTSKARNKIRQF
HHCCCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
FKNQDKELSVNKGREMLMAQFQENGYVANKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGE
HCCCCCCCCHHCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
IGAITVFNRLTEKERREEERAKAKAEAEELVKGGEVKVENKETLKVKHEGGVVIEGASGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCEEEECCCHH
LVRIAKCCNPVPGDDIVGYITKGRGVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNK
HHHHHHHCCCCCCCCCEEEEECCCCEEEEEEHHHCCCHHCCHHHHHCCCCCCCCCCCCCC
EYMAHIDIYGLNRTGLLNDVLQVLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLT
CEEEEEEEEECCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCEEEEEEEEECHHHHHHHH
TVVDKIKSVPEVYSVKRTNG
HHHHHHHCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232196 [H]