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The map label for this gene is hrsA [H]

Identifier: 225861449

GI number: 225861449

Start: 1480525

End: 1480974

Strand: Reverse

Name: hrsA [H]

Synonym: SPT_1559

Alternate gene names: 225861449

Gene position: 1480974-1480525 (Counterclockwise)

Preceding gene: 225861450

Following gene: 225861448

Centisome position: 70.12

GC content: 41.78

Gene sequence:

>450_bases
ATGGAAATCAAAGATATTCTTAATGTGAGTCTGATCCAGACGGATTTACAGATGCAGAGCAAAGAAGAGGTTTTTGAGGC
ATTAGCTCAACTATTGGTTGAGACGGGTTATGTGTCTGATAGAGACCAATTTATCGAAGGTCTTTATCAGAGAGAGGCAG
AAGGACAGACCGGTATTGGGAATTATATTGCTATTCCCCATAGCAAGAGTTCTGCTGTGGAGAAGGCGGGGGTAGTCATA
GCTATAAATCACAATGAGATTCCTTGGGAGACCATTGATGGGAAAGGGGTCAAAGTAATTGTACTCTTTGCAGTTGGTGA
TGATACAGAAGCTGCTAGGGAGCATTTGAAGACCTTATCACTCTTTGCTCGAAAACTTGGTAATGACGAAGTTGTTGCCA
AATTAGTTCGGGCTCAGACATCTGATGATGTGATTGCAGCTTTTTGTTAA

Upstream 100 bases:

>100_bases
GACAAGTCATGTATTAAAACATTGATGAGAAGACTAGCAGATGAATCATTCATAAATCAATTAAAACAGTTAACAAAAGA
AGAATTACGGGAGATAATAT

Downstream 100 bases:

>100_bases
TAAGAAAAAATTTTGGAGGGTATCCGTATGAAAATTGTTGGTGTTGCAGCTTGTACTGTGGGAATTGCCCACACTTATAT
TGCACAGGAAAAATTAGAGA

Product: PTS system, IIA component

Products: NA

Alternate protein names: Putative PTS system EIIABC component; Phosphotransferase enzyme IIA component; PTS system EIIA component; Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component [H]

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQFIEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVI
AINHNEIPWETIDGKGVKVIVLFAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC

Sequences:

>Translated_149_residues
MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQFIEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVI
AINHNEIPWETIDGKGVKVIVLFAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC
>Mature_149_residues
MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQFIEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVI
AINHNEIPWETIDGKGVKVIVLFAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786951, Length=133, Percent_Identity=34.5864661654135, Blast_Score=94, Evalue=3e-21,
Organism=Escherichia coli, GI2367327, Length=140, Percent_Identity=29.2857142857143, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1789597, Length=145, Percent_Identity=31.0344827586207, Blast_Score=67, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 16360; Mature: 16360

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQFIEGLYQREAEGQTGIG
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
NYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVLFAVGDDTEAAREHLKTLS
CEEEECCCCCHHHHHCCEEEEEECCCCCCEEECCCCEEEEEEEEECCCCHHHHHHHHHHH
LFARKLGNDEVVAKLVRAQTSDDVIAAFC
HHHHHCCCHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQFIEGLYQREAEGQTGIG
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
NYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVLFAVGDDTEAAREHLKTLS
CEEEECCCCCHHHHHCCEEEEEECCCCCCEEECCCCEEEEEEEEECCCCHHHHHHHHHHH
LFARKLGNDEVVAKLVRAQTSDDVIAAFC
HHHHHCCCHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9063979; 9278503; 8905232 [H]