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The map label for this gene is metG [H]

Identifier: 225861309

GI number: 225861309

Start: 1344582

End: 1346579

Strand: Reverse

Name: metG [H]

Synonym: SPT_1412

Alternate gene names: 225861309

Gene position: 1346579-1344582 (Counterclockwise)

Preceding gene: 225861310

Following gene: 225861308

Centisome position: 63.75

GC content: 42.19

Gene sequence:

>1998_bases
ATGTCTGAAAAGAATTTTTATATTACAACGCCGATTTACTATCCATCTGGGAAACTTCATATCGGTTCTGCCTACACAAC
TATCGCATGTGATGTCCTAGCACGTTACAAACGCCTGATGGGCTACGATGTATTTTATCTGACAGGTCTTGATGAACATG
GTCAGAAAATCCAGCAGAAAGCGGAAGAAGCTGGTATTACACCTCAAGCCTATGTTGATGGAATGGCGGTTGGAGTTAAA
GAACTCTGGCAATTACTAGATATCTCATACGATAAATTTATCCGTACAACTGATGACTACCATGAAAAAGTTGTTGCACA
GGTCTTTGAACGCTTACTTGCTCAAGATGATATCTACTTGGGTGAATACTCTGGTTGGTATTCAGTATCAGACGAAGAAT
TCTTTACAGAAAGCCAGCTGGCAGAAGTTTTCCGTGATGAAGCTGGAAATGTGACTGGTGGTATTGCTCCATCAGGTCAT
GAGGTTGAATGGGTTTCTGAAGAATCATACTTCCTTCGCCTTAGCAAATACCAAGACCGTTTGGTCGAATTTTTCAAAGC
TCATCCTGAATTTATCACGCCAGATGGTCGCCTTAATGAAATGCTACGCAACTTCATCGAGCCAGGTTTGGAAGATTTGG
CGGTATCTCGTACAACCTTTACATGGGGAGTGCCTGTCCCATCAAATCCAAAACACGTTGTCTACGTTTGGATTGATGCC
CTTCTTAACTATGCGACAGCTCTTGGCTACGCTCAAGACGAACATGGTAACTTTGACAAGTTCTGGAATGGAACAGTCTT
CCATATGGTAGGAAAAGATATCCTTCGCTTCCACTCTATCTACTGGCCAATCCTTCTTATGATGTTGGATGTTAAATTAC
CTGATCGTTTGATTGCCCATGGTTGGTTTGTCATGAAAGACGGAAAAATGTCTAAGTCAAAAGGGAATGTCGTTTACCCT
GAAATGTTGGTAGAGCGTTATGGACTAGATCCACTTCGTTACTACCTCATGCGTAACCTTCCAGTTGGTTCAGACGGAAC
CTTTACTCCTGAAGACTATGTCGGCCGTATCAACTATGAATTGGCTAATGACCTTGGGAACCTCCTTAACCGTACGGTTT
CCATGATTAATAAGTACTTTGATGGACAAATCCCTGCCTATGTAGAAGGTGTGACTGAATTTGATCATGTTCTTGCTGAG
GTTGCAGAACAATCAATCGCAGACTTCCATACACACATGGAAGCAGTTGACTATCCACGTGCGCTTGAAGCAGTCTGGAC
TCTGATCTCTCGTACCAATAAATACATCGATGAGACTGCACCATGGGTCTTGGACAAGGATGAAGCTCTTCGTGACCAAT
TGGCAAGTGTCATGAGCCACTTGGCAGCCAGCATTCGTGTAGTTGCTCACTTGATTGAACCATTTATGATGGAAACTAGT
CGTGCAGTTTTGACTCAACTTGGTTTGGAAGAAGTTTCTAGTCTTGAAAACTTGAGTTTGGCTGACTTCCCAGCAGATGT
GACTGTAGTTGCCAAAGGAACACCTATCTTTCCACGTCTAAATATGGAAGAAGAAATCGCCTATATCAAGGAACAAATGG
AAGGCAATAAACCAGCAGTCGAAAAAGAATGGAATCCGGACGAAGTTGAGCTCAAACTAAACAAGGATGAAATCAAGTTT
GAAGACTTTGACAAGGTTGAAATCCGTGTGGCAGAAGTCAAAGAAGTGTCTAAAGTAGAAGGTTCAGATAAGTTGCTTCA
ATTCCGCTTGGATGCTGGTGATGGAGAAGATCGTCAGATTCTTTCAGGAATTGCAAAATACTATCCAAATGAACAAGAAT
TGGTCGGCAAGAAAGTTCAAATCGTTGCTAACCTCAAACCACGTAAAATGATGAAAAAATATGTCAGCCAGGGTATGATT
CTCTCAGCTGAACATGATGGCAAATTAACCCTTCTCACAGTTGATCCAGCTGTACCAAATGGAAGTGTGATTGGGTAA

Upstream 100 bases:

>100_bases
TATCTTGTTTTTGTATGGATTTCCCTATCGAAATTCATTAAAAATGTGATATAATGAAGTGTTAGAAAAACAGGTTTCAT
TTGCCTGGAAAGGAAAAATT

Downstream 100 bases:

>100_bases
AAGTAGACAGGACTAGTTCAGGCTAGTTCTGTTTTTTTATTTATACTCAATGAAAATCAAAGAGCAAACTAGGAAGCTAG
CCGCAGGTTGCTCAAAACAC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 665; Mature: 664

Protein sequence:

>665_residues
MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDVFYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVK
ELWQLLDISYDKFIRTTDDYHEKVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRTTFTWGVPVPSNPKHVVYVWIDA
LLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYP
EMLVERYGLDPLRYYLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVTEFDHVLAE
VAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLDKDEALRDQLASVMSHLAASIRVVAHLIEPFMMETS
RAVLTQLGLEEVSSLENLSLADFPADVTVVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKF
EDFDKVEIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVANLKPRKMMKKYVSQGMI
LSAEHDGKLTLLTVDPAVPNGSVIG

Sequences:

>Translated_665_residues
MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDVFYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVK
ELWQLLDISYDKFIRTTDDYHEKVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRTTFTWGVPVPSNPKHVVYVWIDA
LLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYP
EMLVERYGLDPLRYYLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVTEFDHVLAE
VAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLDKDEALRDQLASVMSHLAASIRVVAHLIEPFMMETS
RAVLTQLGLEEVSSLENLSLADFPADVTVVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKF
EDFDKVEIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVANLKPRKMMKKYVSQGMI
LSAEHDGKLTLLTVDPAVPNGSVIG
>Mature_664_residues
SEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDVFYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKE
LWQLLDISYDKFIRTTDDYHEKVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGHE
VEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRTTFTWGVPVPSNPKHVVYVWIDAL
LNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPE
MLVERYGLDPLRYYLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVTEFDHVLAEV
AEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLDKDEALRDQLASVMSHLAASIRVVAHLIEPFMMETSR
AVLTQLGLEEVSSLENLSLADFPADVTVVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFE
DFDKVEIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVANLKPRKMMKKYVSQGMIL
SAEHDGKLTLLTVDPAVPNGSVIG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=506, Percent_Identity=33.9920948616601, Blast_Score=283, Evalue=3e-76,
Organism=Homo sapiens, GI14043022, Length=579, Percent_Identity=26.7702936096718, Blast_Score=182, Evalue=8e-46,
Organism=Escherichia coli, GI1788432, Length=695, Percent_Identity=28.4892086330935, Blast_Score=263, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI212645096, Length=504, Percent_Identity=31.9444444444444, Blast_Score=258, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI71992781, Length=396, Percent_Identity=30.8080808080808, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17540794, Length=575, Percent_Identity=25.0434782608696, Blast_Score=165, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6321610, Length=524, Percent_Identity=31.1068702290076, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6321704, Length=522, Percent_Identity=24.5210727969349, Blast_Score=150, Evalue=4e-37,
Organism=Drosophila melanogaster, GI21358483, Length=503, Percent_Identity=32.8031809145129, Blast_Score=259, Evalue=5e-69,
Organism=Drosophila melanogaster, GI24655311, Length=551, Percent_Identity=25.7713248638838, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75696; Mature: 75565

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDVFYLTGLDEHGQKIQQK
CCCCCEEEECCEEECCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
AEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHEKVVAQVFERLLAQDDIYL
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
GEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGHEVEWVSEESYFLRLSKYQDR
ECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHH
LVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRTTFTWGVPVPSNPKHVVYVWIDA
HHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHEECEEEEECCCCCCCCCEEEEEHHHH
LLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDVKLPDRLIAH
HHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
GWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRYYLMRNLPVGSDGTFTPEDYVGRINYE
CEEEEECCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHEHHH
LANDLGNLLNRTVSMINKYFDGQIPAYVEGVTEFDHVLAEVAEQSIADFHTHMEAVDYPR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ALEAVWTLISRTNKYIDETAPWVLDKDEALRDQLASVMSHLAASIRVVAHLIEPFMMETS
HHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAVLTQLGLEEVSSLENLSLADFPADVTVVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAV
HHHHHHHCHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCC
EKEWNPDEVELKLNKDEIKFEDFDKVEIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQI
CCCCCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCEEEEEECCCCCCHHHH
LSGIAKYYPNEQELVGKKVQIVANLKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPN
HHHHHHHCCCHHHHCCCCEEEEECCCHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCC
GSVIG
CCCCC
>Mature Secondary Structure 
SEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDVFYLTGLDEHGQKIQQK
CCCCEEEECCEEECCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
AEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHEKVVAQVFERLLAQDDIYL
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
GEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGHEVEWVSEESYFLRLSKYQDR
ECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHH
LVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRTTFTWGVPVPSNPKHVVYVWIDA
HHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHEECEEEEECCCCCCCCCEEEEEHHHH
LLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDVKLPDRLIAH
HHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
GWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRYYLMRNLPVGSDGTFTPEDYVGRINYE
CEEEEECCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHEHHH
LANDLGNLLNRTVSMINKYFDGQIPAYVEGVTEFDHVLAEVAEQSIADFHTHMEAVDYPR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ALEAVWTLISRTNKYIDETAPWVLDKDEALRDQLASVMSHLAASIRVVAHLIEPFMMETS
HHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAVLTQLGLEEVSSLENLSLADFPADVTVVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAV
HHHHHHHCHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCC
EKEWNPDEVELKLNKDEIKFEDFDKVEIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQI
CCCCCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCEEEEEECCCCCCHHHH
LSGIAKYYPNEQELVGKKVQIVANLKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPN
HHHHHHHCCCHHHHCCCCEEEEECCCHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCC
GSVIG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]