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The map label for this gene is gloA [H]

Identifier: 225861151

GI number: 225861151

Start: 1184471

End: 1184851

Strand: Reverse

Name: gloA [H]

Synonym: SPT_1241

Alternate gene names: 225861151

Gene position: 1184851-1184471 (Counterclockwise)

Preceding gene: 225861152

Following gene: 225861150

Centisome position: 56.1

GC content: 40.68

Gene sequence:

>381_bases
ATGGCTTCAAAAATGCTACACACTTGCTTACGAGTAGAAAATCTTGAAAAATCAATCGCATTTTATCAAGATGCTTTTGG
TTTTAAAGAATTGCGTCGCAGAGATTTTCCAGACCATGCCTTCACGATTGTCTATCTAGGTCTTGAGGGTGATGATTATG
AGTTGGAGTTGACTTATAACTACGATCACGGTCCGTATGTGGTTGGTGATGGGTTTGCCCATATCGCCCTCAGTACACCT
GATCTTGAGGCACTTCATCAAGAACACAGTGCAAAAGGCTATGAAGTGACTGAGCCAAATGGTCTACCAGGAACTACACC
AAACTATTACTTTGTTAAAGACCCTGATGGATATAAGGTCGAAGTCATTCGTGAAAAATAA

Upstream 100 bases:

>100_bases
CACAGCTCTTGCAGATGCAGCTAAAGCAAAACTTGGTAAATAACAACTGATGAGACTGGAACAGGATTGTCCAGTCTTTT
TAAAAATAAAGGAGAAAGAT

Downstream 100 bases:

>100_bases
TTTCGTGCAAGTTAAGTAGAATATTCGTTTTCTACTTGACTTTTTTTAGCTGAAAGAGTATACTAATAAAAATTCAACCT
TTAAGGGGAGTCCAGAGAGA

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIVYLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTP
DLEALHQEHSAKGYEVTEPNGLPGTTPNYYFVKDPDGYKVEVIREK

Sequences:

>Translated_126_residues
MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIVYLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTP
DLEALHQEHSAKGYEVTEPNGLPGTTPNYYFVKDPDGYKVEVIREK
>Mature_125_residues
ASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIVYLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPD
LEALHQEHSAKGYEVTEPNGLPGTTPNYYFVKDPDGYKVEVIREK

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Escherichia coli, GI1787940, Length=126, Percent_Identity=38.0952380952381, Blast_Score=88, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323639, Length=142, Percent_Identity=31.6901408450704, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14438; Mature: 14307

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIVYLGLEGDDYELELTYN
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEECCCCCEEEEEEEE
YDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGLPGTTPNYYFVKDPDGYKV
CCCCCEEEECCEEEEEEECCCHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEECCCCCEE
EVIREK
EEEECC
>Mature Secondary Structure 
ASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIVYLGLEGDDYELELTYN
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEECCCCCEEEEEEEE
YDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGLPGTTPNYYFVKDPDGYKV
CCCCCEEEECCEEEEEEECCCHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEECCCCCEE
EVIREK
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]