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The map label for this gene is nag3 [H]

Identifier: 225859881

GI number: 225859881

Start: 2043062

End: 2044852

Strand: Reverse

Name: nag3 [H]

Synonym: SP70585_2234

Alternate gene names: 225859881

Gene position: 2044852-2043062 (Counterclockwise)

Preceding gene: 225859882

Following gene: 225859880

Centisome position: 93.6

GC content: 43.27

Gene sequence:

>1791_bases
ATGACACATTTGGTAGACTTACGGAAGAAACCTTATAACCTTGATGAAGAAGCTATTTCATGGGTTGAAAATACGATTGC
TCAGATGACATTGGATGAAAAAATAGGACATCTCTTTGTCAACATGGGTATCCAGCGGACGGAGAAATATCTGACAGGAG
TATTGGAAGATTACAAGATTGCTGCTGTTCGGTATAATCCAGGGCCAGCGGCAGATATTTGGGAACAAAACTATATTTTG
CAAACTAAGTCCAAAATTCCTCTCTTAATAGCAGCAAACACAGAATCTGGTGGAAATGGTGCTGTTACAGATGGAACAAA
AATTGGCGATGAAATCAAAATTGCAGCAACAGCTGATTCTCGCCATGCTTATGAGATGGGGAAAGTTGCAGGGATTGAAG
CAGCAGCAGTAGGCTGTAACGCTTCCTTTGCACCGATTATGGATCTTAGCCGCAACTGGCGAAATCCAATTATCGCCAAT
CGTACTTGGGGAGCAGATGTTGACCAAGTGATTGAACTGTCTAAAGAATACATGCGTGGGATTATGGAACATGGCATTGT
GCCTTTTGCGAAGCATTTTCCTGGCGATGGTATTGATGAACGCGATCACCACCTCTCTTTTGCTTCTAATCCAATGACAA
AATCTGAATGGATGGAAACCTTTGGACGGATTTACGGTGAAATGGCTGATGCAGGTCTTCCAGGTATCATGGCGGGGCAC
ATTCATTTGCCAAATGTTGAGAAAGAAATGCATCCAGAACGTGAATTGGATGATATGTTGCCAGCTTCGTTGAATAAAAC
CCTCTTGGATGAATTGTTGCGTGGGGAGTTAGGCTACAACGGCGCAATTGTGACGGATGCTAGTCACATGATTGGTATGA
CAGCATCTATGCCACGTCGTGAGCTCTTACCGACAGCTATTGAAGCGGGCTGCGATCTCTTCCTCTTTTTCAATGATCCT
GAAGAAGATTTGCAATGGATGAAAGAGGGGTTGAAAAATGGACTTCTCACTGAGGAGCGGCTCCATGATGCTCTTCGTCG
GACCTTGGGCTTGAAAGCTAAACTCGGCCTCCACCTTTTTGAAGGTCGTCGTCAGGAAATTATGTTGCCAAAAGAAGAAG
CGCTGGCTTTAATTGGAAAAGATGAGGCTAAAAATCTTGCAAAAGAAGTAGCTGACAAGGCTATTACCTTGGTAAAATCC
AAGCAAGAAGGCATTTTCCCAGTCAATCCAGATCGCTATAAACGGATTTTATTGGTTGAAGTCGACGGCTACAAAGGTGG
CTTTGGTGCCATGATTAATGCAGGAAAGAAGAGGGCGGCTGATACGCTAAAAGAATTGTTAGAAGCACGAGGTCACGAAG
TGTCTATCTGGGAAAATACAGAAGCAAGGATTGCCAAATTACCAGAAGAAGAAAGACCAGCTGCGATTGCTAATGTCTAT
GCATCTAAGCGTCCTATTGCAGAAATCACAGATAACTATGATTTGATTATCAATTTGGTTGATGTCAATTCAGGTGGAAC
AACTCAACGAATTATTTGGCCTGCTGCCAAGGGAACACCAGATCAGCCGTTCTATGTTCATGAAATACCAACCATCGTTG
TTTCTGTTCAGCATCCCTTTGCTCTGGCAGATATGCCACAAGTAGCAACCTATATCAATGCCTATGATGGTTTACCAGTA
ACTTTGGAAACCTTAGTTACCAAACTAGCAGGAGAATCATCGTTTACAGGAGTGTCCCCTGTTGATGCTTACTGTGGCTT
GGTAGATACACATATTTGGCGTGGGAATTAA

Upstream 100 bases:

>100_bases
GGTGAGAAAGAAACAAACTTGATACAAGCATTTTGGAAAAATTTAGACGACTAGATAAGGGCTAGAGGCTACATGCCCTC
TAGCTTTTTTGGAGGCAACG

Downstream 100 bases:

>100_bases
GGAGACAGCAATGCCTAGACTAGTTGATTTAAGAAAGAAACCATATTACTTGAATGATGATCAGATTGTCTGGGTAGAGA
GTACCCTTGAAAATCTGACA

Product: beta-N-acetylglucosaminidase/beta-glucosidase (3-beta-N-acetyl-D-glucosaminidase/beta-D- glucosidase)(Nag3)

Products: NA

Alternate protein names: 3-beta-N-acetyl-D-glucosaminidase/beta-D-glucosidase; Nag3 [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MTHLVDLRKKPYNLDEEAISWVENTIAQMTLDEKIGHLFVNMGIQRTEKYLTGVLEDYKIAAVRYNPGPAADIWEQNYIL
QTKSKIPLLIAANTESGGNGAVTDGTKIGDEIKIAATADSRHAYEMGKVAGIEAAAVGCNASFAPIMDLSRNWRNPIIAN
RTWGADVDQVIELSKEYMRGIMEHGIVPFAKHFPGDGIDERDHHLSFASNPMTKSEWMETFGRIYGEMADAGLPGIMAGH
IHLPNVEKEMHPERELDDMLPASLNKTLLDELLRGELGYNGAIVTDASHMIGMTASMPRRELLPTAIEAGCDLFLFFNDP
EEDLQWMKEGLKNGLLTEERLHDALRRTLGLKAKLGLHLFEGRRQEIMLPKEEALALIGKDEAKNLAKEVADKAITLVKS
KQEGIFPVNPDRYKRILLVEVDGYKGGFGAMINAGKKRAADTLKELLEARGHEVSIWENTEARIAKLPEEERPAAIANVY
ASKRPIAEITDNYDLIINLVDVNSGGTTQRIIWPAAKGTPDQPFYVHEIPTIVVSVQHPFALADMPQVATYINAYDGLPV
TLETLVTKLAGESSFTGVSPVDAYCGLVDTHIWRGN

Sequences:

>Translated_596_residues
MTHLVDLRKKPYNLDEEAISWVENTIAQMTLDEKIGHLFVNMGIQRTEKYLTGVLEDYKIAAVRYNPGPAADIWEQNYIL
QTKSKIPLLIAANTESGGNGAVTDGTKIGDEIKIAATADSRHAYEMGKVAGIEAAAVGCNASFAPIMDLSRNWRNPIIAN
RTWGADVDQVIELSKEYMRGIMEHGIVPFAKHFPGDGIDERDHHLSFASNPMTKSEWMETFGRIYGEMADAGLPGIMAGH
IHLPNVEKEMHPERELDDMLPASLNKTLLDELLRGELGYNGAIVTDASHMIGMTASMPRRELLPTAIEAGCDLFLFFNDP
EEDLQWMKEGLKNGLLTEERLHDALRRTLGLKAKLGLHLFEGRRQEIMLPKEEALALIGKDEAKNLAKEVADKAITLVKS
KQEGIFPVNPDRYKRILLVEVDGYKGGFGAMINAGKKRAADTLKELLEARGHEVSIWENTEARIAKLPEEERPAAIANVY
ASKRPIAEITDNYDLIINLVDVNSGGTTQRIIWPAAKGTPDQPFYVHEIPTIVVSVQHPFALADMPQVATYINAYDGLPV
TLETLVTKLAGESSFTGVSPVDAYCGLVDTHIWRGN
>Mature_595_residues
THLVDLRKKPYNLDEEAISWVENTIAQMTLDEKIGHLFVNMGIQRTEKYLTGVLEDYKIAAVRYNPGPAADIWEQNYILQ
TKSKIPLLIAANTESGGNGAVTDGTKIGDEIKIAATADSRHAYEMGKVAGIEAAAVGCNASFAPIMDLSRNWRNPIIANR
TWGADVDQVIELSKEYMRGIMEHGIVPFAKHFPGDGIDERDHHLSFASNPMTKSEWMETFGRIYGEMADAGLPGIMAGHI
HLPNVEKEMHPERELDDMLPASLNKTLLDELLRGELGYNGAIVTDASHMIGMTASMPRRELLPTAIEAGCDLFLFFNDPE
EDLQWMKEGLKNGLLTEERLHDALRRTLGLKAKLGLHLFEGRRQEIMLPKEEALALIGKDEAKNLAKEVADKAITLVKSK
QEGIFPVNPDRYKRILLVEVDGYKGGFGAMINAGKKRAADTLKELLEARGHEVSIWENTEARIAKLPEEERPAAIANVYA
SKRPIAEITDNYDLIINLVDVNSGGTTQRIIWPAAKGTPDQPFYVHEIPTIVVSVQHPFALADMPQVATYINAYDGLPVT
LETLVTKLAGESSFTGVSPVDAYCGLVDTHIWRGN

Specific function: Catalyzes the cleavage of beta-N-acetyl-D-glucosaminides and beta-D-glucosides. Might be involved in the degradation of glucuronic acid-containing glycosaminoglycans such as hyaluronic acid [H]

COG id: COG1472

COG function: function code G; Beta-glucosidase-related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787350, Length=249, Percent_Identity=26.5060240963855, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788453, Length=407, Percent_Identity=23.0958230958231, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019800
- InterPro:   IPR001764
- InterPro:   IPR017853 [H]

Pfam domain/function: PF00933 Glyco_hydro_3 [H]

EC number: =3.2.1.21; =3.2.1.52 [H]

Molecular weight: Translated: 65943; Mature: 65812

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHLVDLRKKPYNLDEEAISWVENTIAQMTLDEKIGHLFVNMGIQRTEKYLTGVLEDYKI
CCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEE
AAVRYNPGPAADIWEQNYILQTKSKIPLLIAANTESGGNGAVTDGTKIGDEIKIAATADS
EEEEECCCCCHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCC
RHAYEMGKVAGIEAAAVGCNASFAPIMDLSRNWRNPIIANRTWGADVDQVIELSKEYMRG
CHHHHHHHHCCCHHEEECCCCCCHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHH
IMEHGIVPFAKHFPGDGIDERDHHLSFASNPMTKSEWMETFGRIYGEMADAGLPGIMAGH
HHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECC
IHLPNVEKEMHPERELDDMLPASLNKTLLDELLRGELGYNGAIVTDASHMIGMTASMPRR
EECCCCHHHCCCCHHHHHHCHHHCCHHHHHHHHHCCCCCCCEEEECCHHHHCCCCCCCHH
ELLPTAIEAGCDLFLFFNDPEEDLQWMKEGLKNGLLTEERLHDALRRTLGLKAKLGLHLF
HHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHCHHHH
EGRRQEIMLPKEEALALIGKDEAKNLAKEVADKAITLVKSKQEGIFPVNPDRYKRILLVE
CCCCCEECCCHHHHHEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
VDGYKGGFGAMINAGKKRAADTLKELLEARGHEVSIWENTEARIAKLPEEERPAAIANVY
ECCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCHHCCHHHHHHH
ASKRPIAEITDNYDLIINLVDVNSGGTTQRIIWPAAKGTPDQPFYVHEIPTIVVSVQHPF
HCCCCHHHHCCCCEEEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCEEEEEECCCC
ALADMPQVATYINAYDGLPVTLETLVTKLAGESSFTGVSPVDAYCGLVDTHIWRGN
CCCCCHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEECCC
>Mature Secondary Structure 
THLVDLRKKPYNLDEEAISWVENTIAQMTLDEKIGHLFVNMGIQRTEKYLTGVLEDYKI
CCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEE
AAVRYNPGPAADIWEQNYILQTKSKIPLLIAANTESGGNGAVTDGTKIGDEIKIAATADS
EEEEECCCCCHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCC
RHAYEMGKVAGIEAAAVGCNASFAPIMDLSRNWRNPIIANRTWGADVDQVIELSKEYMRG
CHHHHHHHHCCCHHEEECCCCCCHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHH
IMEHGIVPFAKHFPGDGIDERDHHLSFASNPMTKSEWMETFGRIYGEMADAGLPGIMAGH
HHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECC
IHLPNVEKEMHPERELDDMLPASLNKTLLDELLRGELGYNGAIVTDASHMIGMTASMPRR
EECCCCHHHCCCCHHHHHHCHHHCCHHHHHHHHHCCCCCCCEEEECCHHHHCCCCCCCHH
ELLPTAIEAGCDLFLFFNDPEEDLQWMKEGLKNGLLTEERLHDALRRTLGLKAKLGLHLF
HHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHCHHHH
EGRRQEIMLPKEEALALIGKDEAKNLAKEVADKAITLVKSKQEGIFPVNPDRYKRILLVE
CCCCCEECCCHHHHHEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
VDGYKGGFGAMINAGKKRAADTLKELLEARGHEVSIWENTEARIAKLPEEERPAAIANVY
ECCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCHHCCHHHHHHH
ASKRPIAEITDNYDLIINLVDVNSGGTTQRIIWPAAKGTPDQPFYVHEIPTIVVSVQHPF
HCCCCHHHHCCCCEEEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCEEEEEECCCC
ALADMPQVATYINAYDGLPVTLETLVTKLAGESSFTGVSPVDAYCGLVDTHIWRGN
CCCCCHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA