Click here to switch to the map view.

The map label for this gene is epsJ [H]

Identifier: 225859516

GI number: 225859516

Start: 1676046

End: 1676951

Strand: Reverse

Name: epsJ [H]

Synonym: SP70585_1805

Alternate gene names: 225859516

Gene position: 1676951-1676046 (Counterclockwise)

Preceding gene: 225859517

Following gene: 225859515

Centisome position: 76.76

GC content: 34.11

Gene sequence:

>906_bases
ATGAGTGAATTAATTAGTGTTGTGGTACCGATATACAATACGGGAAAATATTTAGTGGAGTGTGTCGAGCATATTCTGAA
GCAAACCTATCAAAATATAGAAATTATTTTAGTTGATGACAGTTCTACGGATAATTCTGGGGAAATTTGTGATGCTTTTA
TGATGCAAGATAATCGTGTGCGAGTATTGCATCAAGAAAATAAGGGGGGGGCAGCACAAGCTAAAAATATGGGGATTAGT
GTAGCTAAGGGAGAGTACATCACGATTGTTGATTCAGATGATATCGTAAAAGAAAATATGATTGAAACTCTTTATCAGCA
AGTCCAAGAAAAGGATGCGGATGTTGTTATAGGGAATTACTATAATTATGACGAAAGTGACGGGAATTTTTATTTTTATG
TAACAGGGCAAGATTTTTGCGTCGAAGAATTAGCTATACAAGAAATTATGAACCGTCAAGCAGGAGATTGGAAATTCAAT
AGCTCGGCCTTTATATTGCCGACATTTAAGTTGATTAAAAAAGAATTATTCAATGAAGTTCACTTTTCAAATGGTCGCCG
CTTTGATGATGAAGCAACTATGCATCGCTTTTATCTTTTAGCCTCTAAAATCGTCTTTATAAACGATAATCTCTATCTGT
ATAGAAGACGTTCAGGAAGCATCATGAGAACGGAATTTGATCTTTCCTGGGCAAGAGATATTGTTGAAGTGTTTTCTAAG
AAAATATCGGATTGTATCTTGGCTGGTTTGGATGTCTCCGTTCTGCGTATTCGATTTGTCAATCTTTTAAAAGATTATAA
GCAAACTTTAGAATACCATCAATTAACAGATACTGAGGAATATAAAGATATTTGTTTCAGATTAAAGTTGTTTTTTGATG
CAGAACAAAGAAATGGTAAAAGTTGA

Upstream 100 bases:

>100_bases
TCACACGTAAAAGTATGGATGATGGACTCTATGACGGTATTTTTTCTGTGGAGAGACCAGATGATTTAGTGGATAGAATG
AAGAATATAGAGATAGAGTA

Downstream 100 bases:

>100_bases
AATAAAAGAATTGTTATTTACCATATCACAAACAATGAAGGTGAGGGGAGTGTTTTATGACTAAGATTTATTCGTCAATA
GCAGTAAAAAAAGGACTATT

Product: glycosyl transferase, family 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDSSTDNSGEICDAFMMQDNRVRVLHQENKGGAAQAKNMGIS
VAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNYYNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFN
SSAFILPTFKLIKKELFNEVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVEVFSK
KISDCILAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDAEQRNGKS

Sequences:

>Translated_301_residues
MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDSSTDNSGEICDAFMMQDNRVRVLHQENKGGAAQAKNMGIS
VAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNYYNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFN
SSAFILPTFKLIKKELFNEVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVEVFSK
KISDCILAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDAEQRNGKS
>Mature_300_residues
SELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDSSTDNSGEICDAFMMQDNRVRVLHQENKGGAAQAKNMGISV
AKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNYYNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNS
SAFILPTFKLIKKELFNEVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVEVFSKK
ISDCILAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDAEQRNGKS

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790044, Length=135, Percent_Identity=39.2592592592593, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787259, Length=92, Percent_Identity=36.9565217391304, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788372, Length=111, Percent_Identity=32.4324324324324, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 35055; Mature: 34924

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDSSTDNSGEICDAFMMQDNRV
CCCHHEEHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHCCCCE
RVLHQENKGGAAQAKNMGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
EEEEECCCCCCCHHHCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEECC
YNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFNEV
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEECCCEEEEHHHHHHHHHHHHHH
HFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVEVFSK
HCCCCCCCCHHHHHHHHHHHHCEEEEEECCEEEEEECCCCEEEEECCHHHHHHHHHHHHH
KISDCILAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDAEQRNGK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEECHHCCCC
S
C
>Mature Secondary Structure 
SELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDSSTDNSGEICDAFMMQDNRV
CCHHEEHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHCCCCE
RVLHQENKGGAAQAKNMGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
EEEEECCCCCCCHHHCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEECC
YNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFNEV
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEECCCEEEEHHHHHHHHHHHHHH
HFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVEVFSK
HCCCCCCCCHHHHHHHHHHHHCEEEEEECCEEEEEECCCCEEEEECCHHHHHHHHHHHHH
KISDCILAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDAEQRNGK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEECHHCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]