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The map label for this gene is norM [H]

Identifier: 225858952

GI number: 225858952

Start: 1129000

End: 1130361

Strand: Reverse

Name: norM [H]

Synonym: SP70585_1217

Alternate gene names: 225858952

Gene position: 1130361-1129000 (Counterclockwise)

Preceding gene: 225858953

Following gene: 225858951

Centisome position: 51.74

GC content: 42.22

Gene sequence:

>1362_bases
ATGTATAAGACAAAGTGTTTACGAGAGAAGTTGGTATTATTTTTAAAAATTTTCTTCCCAATCCTGATTTACCAATTTGC
CAATTATTCTGCCTCTTTTGTTGATACTGCAATGACAGGTCAATACAACACTATGGACTTGACTGGTGTATCTATGGCAA
CCAGTATCTGGAATCCTTTCTTTACATTTCTAACAGGGATTGTGTCAGCCTTGGTGCCTATCATTGGTCACCATCTTGGT
CGAGGAAAAAAGGAAGAAGTTGCGTCTGATTTTTACCAATTTATTTATTTGGCCTTGGGCCTATCTGTGGTCTTGCTGGG
GATGGTACTTTTCTTGGCACCAACAATCTTGAATCATATTGGGTTAGAAGCAGCAGTAGCGGCAGTAGCGGTTCGCTATC
TTTGGTTTTTATCTATCGGGATTATCCCCTTGTTGCTCTTTAGCGTCATTCGTTCCTTGCTGGATTCGCTGGGCTTGACC
AAACTGTCCATGTACCTCATGCTTTTGTTACTCCCTCTCAATAGCGGATTTAACTATCTCTTGATTTACGGTGCCTTTGG
TGTTCCAGAACTGGGAGGGGCTGGTGCTGGTTTAGGAACATCCTTGGCCTACTGGGTCTTGCTTGGGATTTCTGTTCTGG
TTTTATTTAAACAGGAGAAGCTCAAAGCCTTACACCTTGAGAAACGAATTCCACTTAATATGGATAAAATTAAGGAAGGA
GTTCGTTTAGGTCTGCCTATTGGGGGAACTGTCTTCGCGGAAGTGGCTGTCTTTTCAGTGGTTGGCTTGATTATGGCTAA
GTTTTCGTCCTTGATTATAGCTAGTCACCAGTCAGCTATGAACTTTTCAAGTCTTATGTACGCCTTTCCTATGAGTATCT
CATCGGCTATGGCTATTGTCGTTTCCTATGAAGTGGGAGCCAAGCGATTTGATGATGCGAAAACCTATATTGGTCTAGGA
AGATGGACTGCCCTCATTTTTGCGGCCTTCACCTTAACCTTCCTTTACATTTTTAGGGGAAATGTGGCCAGTCTTTATGG
TAACGACCCAAAATTTATCGATTTGACAGCGCGTTTTTTAACTTATAGTCTTTTCTTCCAGTTAGCAGATACCTTTGCGG
CGCCGCTTCAGGGAATTTTGCGGGGGTATAAGGATACAGTTATTCCTTTTTACCTTGGTTTGCTTGGTTATTGGGGCGTA
GCAATCCCTGTGGCTACGCTATTTGATTCCCTAACAGATTTTGGAGCTTATTCTTACTGGATCGGCTTGATTATTAGTTT
GATTGTGAGCGGGGCGCTCTACCGTTGGCGTTTAACTGTGATTATGAAGAGATTTGAATCTTTAGCAAAATCCAAACGCT
AA

Upstream 100 bases:

>100_bases
TGTTGGCATACTACTTCAAAATCTAATAAACCTCCGCTAAGTTTGAAAAACTATGGTCTGAAACTCTTTGGTTTAGATAA
ATCAGAATAGAAAGAGAAGT

Downstream 100 bases:

>100_bases
AAAGTTTGTGAAAAAATATTCTTGACAAGAAAATCCCTTGTTTTCACGGGGTTTTCTTTTTTATTTTGTGAAATTTCTTT
TAGAAAAACTTTGACAGTAT

Product: mate efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNTMDLTGVSMATSIWNPFFTFLTGIVSALVPIIGHHLG
RGKKEEVASDFYQFIYLALGLSVVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQEKLKALHLEKRIPLNMDKIKEG
VRLGLPIGGTVFAEVAVFSVVGLIMAKFSSLIIASHQSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLG
RWTALIFAAFTLTFLYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYLGLLGYWGV
AIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAKSKR

Sequences:

>Translated_453_residues
MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNTMDLTGVSMATSIWNPFFTFLTGIVSALVPIIGHHLG
RGKKEEVASDFYQFIYLALGLSVVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQEKLKALHLEKRIPLNMDKIKEG
VRLGLPIGGTVFAEVAVFSVVGLIMAKFSSLIIASHQSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLG
RWTALIFAAFTLTFLYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYLGLLGYWGV
AIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAKSKR
>Mature_453_residues
MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNTMDLTGVSMATSIWNPFFTFLTGIVSALVPIIGHHLG
RGKKEEVASDFYQFIYLALGLSVVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQEKLKALHLEKRIPLNMDKIKEG
VRLGLPIGGTVFAEVAVFSVVGLIMAKFSSLIIASHQSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLG
RWTALIFAAFTLTFLYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYLGLLGYWGV
AIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAKSKR

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Escherichia coli, GI48994890, Length=441, Percent_Identity=34.2403628117914, Blast_Score=229, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6321821, Length=265, Percent_Identity=23.7735849056604, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320545, Length=388, Percent_Identity=22.680412371134, Blast_Score=63, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 50075; Mature: 50075

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]