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The map label for this gene is gshB [H]

Identifier: 225853559

GI number: 225853559

Start: 2073905

End: 2074843

Strand: Direct

Name: gshB [H]

Synonym: BMEA_A2193

Alternate gene names: 225853559

Gene position: 2073905-2074843 (Clockwise)

Preceding gene: 225853557

Following gene: 225853560

Centisome position: 97.56

GC content: 58.68

Gene sequence:

>939_bases
ATGGCTCTCAAAGTCGCGGTCCAGATGGACCATATCAGCACGGTGAACATCACGGGCGATACCACTTTCGCTCTCTCGCT
TGAGGCGCAAAAGCGCGGCCACGAGCTTTTCCATTATACGCCCGACTGGCTTTCCATGCGCGATGGCGTGGTTTCCGCCC
GCGTGGAAAAGATGGAAGTGCGCGATGTGAAGGGCGATCATTACACGCTGGGCGAGCCTGTCCGCCGCGACCTCACGGAA
ATGGATGTGATCCTGCTGCGCCAGGATCCGCCTTTTGACATGAATTACATCACCACGACGCATCTTCTGGAACGCATCCA
TCCAAAGACGCTGGTGGTCAACGATCCGGCCTGGGTGCGCAACAGCCCGGAAAAGATTTTTGTCACCGAATTTCCCGACC
TGATGCCGGAAACGCTCATCACCAAGGATCCGCAGGAGGTGATGGATTTCCGCCGTGAATTCGGCGACATCATCCTGAAG
CCGCTTTACGGCAATGGCGGCGCGGGCGTCTTCCATCTGGCGGATGGCGACCGCAACCTTACCTCGCTTCTGGAAATGTT
CGGCCAGCTTTTCAGGGAGCCGTTCATCGCGCAGCGCTATCTGAAAGATGTGCGGGCGGGCGACAAGCGTATCATCCTCA
TCGATGGCGAGCCGGTGGGCGCGCTGAACCGCGTGCCGTCCGAAACCGACGCACGCTCCAACATGCATGTGGGCGGACGC
CCCGAACAGAGCAAGTTGACCCCGCGTGAGCGTGAAATCTGCGCGCGCATTGGCCCCTCGCTGAAAGAGCGCGGCTTCAT
CCTCGTCGGCATCGACGTGATTGGCGACTACATGACGGAAATCAACGTGACGTCGCCGACAGGTATCCGTGAAATCGAAC
GTTTTGACGGCACCAATATTGCCGCCCTGTTCTGGGATGCGGTGGAAGCCAGACGATAA

Upstream 100 bases:

>100_bases
TCTATCTCTTTGTTTTGTCGCATTTAGCTTACGCAAAACCGCTTCGCACTTTTGCTGGAAATGCTCTAGATTTCCGGCAA
CTTCGAATCGCGAGGAAATC

Downstream 100 bases:

>100_bases
TTTGTTCCTGAAACGTTCTTGACCTCACCAATAGTATATGCTTAACTCCAGAAGTTGTATTTAAGCATATACTCACTTGT
AGGATGTGCTGCGTGGCAAG

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MALKVAVQMDHISTVNITGDTTFALSLEAQKRGHELFHYTPDWLSMRDGVVSARVEKMEVRDVKGDHYTLGEPVRRDLTE
MDVILLRQDPPFDMNYITTTHLLERIHPKTLVVNDPAWVRNSPEKIFVTEFPDLMPETLITKDPQEVMDFRREFGDIILK
PLYGNGGAGVFHLADGDRNLTSLLEMFGQLFREPFIAQRYLKDVRAGDKRIILIDGEPVGALNRVPSETDARSNMHVGGR
PEQSKLTPREREICARIGPSLKERGFILVGIDVIGDYMTEINVTSPTGIREIERFDGTNIAALFWDAVEARR

Sequences:

>Translated_312_residues
MALKVAVQMDHISTVNITGDTTFALSLEAQKRGHELFHYTPDWLSMRDGVVSARVEKMEVRDVKGDHYTLGEPVRRDLTE
MDVILLRQDPPFDMNYITTTHLLERIHPKTLVVNDPAWVRNSPEKIFVTEFPDLMPETLITKDPQEVMDFRREFGDIILK
PLYGNGGAGVFHLADGDRNLTSLLEMFGQLFREPFIAQRYLKDVRAGDKRIILIDGEPVGALNRVPSETDARSNMHVGGR
PEQSKLTPREREICARIGPSLKERGFILVGIDVIGDYMTEINVTSPTGIREIERFDGTNIAALFWDAVEARR
>Mature_311_residues
ALKVAVQMDHISTVNITGDTTFALSLEAQKRGHELFHYTPDWLSMRDGVVSARVEKMEVRDVKGDHYTLGEPVRRDLTEM
DVILLRQDPPFDMNYITTTHLLERIHPKTLVVNDPAWVRNSPEKIFVTEFPDLMPETLITKDPQEVMDFRREFGDIILKP
LYGNGGAGVFHLADGDRNLTSLLEMFGQLFREPFIAQRYLKDVRAGDKRIILIDGEPVGALNRVPSETDARSNMHVGGRP
EQSKLTPREREICARIGPSLKERGFILVGIDVIGDYMTEINVTSPTGIREIERFDGTNIAALFWDAVEARR

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=311, Percent_Identity=42.443729903537, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI1787076, Length=173, Percent_Identity=30.0578034682081, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35416; Mature: 35285

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKVAVQMDHISTVNITGDTTFALSLEAQKRGHELFHYTPDWLSMRDGVVSARVEKMEV
CEEEEEEEECCEEEEEECCCEEEEEEEEHHHCCCEEEEECCHHHHHHCCHHHHHHHHHHH
RDVKGDHYTLGEPVRRDLTEMDVILLRQDPPFDMNYITTTHLLERIHPKTLVVNDPAWVR
EECCCCCEECCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHC
NSPEKIFVTEFPDLMPETLITKDPQEVMDFRREFGDIILKPLYGNGGAGVFHLADGDRNL
CCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCCCH
TSLLEMFGQLFREPFIAQRYLKDVRAGDKRIILIDGEPVGALNRVPSETDARSNMHVGGR
HHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCCCC
PEQSKLTPREREICARIGPSLKERGFILVGIDVIGDYMTEINVTSPTGIREIERFDGTNI
CCCCCCCCHHHHHHHHHCCCHHHCCEEEEEHHHHHHHHHEEECCCCCCHHHHHHCCCCCE
AALFWDAVEARR
EHHHHHHHHCCC
>Mature Secondary Structure 
ALKVAVQMDHISTVNITGDTTFALSLEAQKRGHELFHYTPDWLSMRDGVVSARVEKMEV
EEEEEEEECCEEEEEECCCEEEEEEEEHHHCCCEEEEECCHHHHHHCCHHHHHHHHHHH
RDVKGDHYTLGEPVRRDLTEMDVILLRQDPPFDMNYITTTHLLERIHPKTLVVNDPAWVR
EECCCCCEECCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHC
NSPEKIFVTEFPDLMPETLITKDPQEVMDFRREFGDIILKPLYGNGGAGVFHLADGDRNL
CCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCCCH
TSLLEMFGQLFREPFIAQRYLKDVRAGDKRIILIDGEPVGALNRVPSETDARSNMHVGGR
HHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCCCC
PEQSKLTPREREICARIGPSLKERGFILVGIDVIGDYMTEINVTSPTGIREIERFDGTNI
CCCCCCCCHHHHHHHHHCCCHHHCCEEEEEHHHHHHHHHEEECCCCCCHHHHHHCCCCCE
AALFWDAVEARR
EHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]