BacMap

Definition	Brucella melitensis ATCC 23457 chromosome chromosome I, complete sequence.
Accession	NC_012441
Length	2,125,701

Click here to switch to the map view.

The map label for this gene is paaF [C]

Identifier: 225853477

GI number: 225853477

Start: 1988906

End: 1989682

Strand: Reverse

Name: paaF [C]

Synonym: BMEA_A2107

Alternate gene names: 225853477

Gene position: 1989682-1988906 (Counterclockwise)

Preceding gene: 225853478

Following gene: 225853476

Centisome position: 93.6

GC content: 61.0

Gene sequence:

>777_bases
ATGGCTGACAGAATAGAAATTTCGATCGAAAACCTCATCGCGGTCATGACAATCAGGCGGCCGGAAAAGCTCAATGCGCT
CGATATACCGCTCTTGCAGGAACTCTCTACCGCCTGTGATCAGGTCGAGGCGGACAGTAGCGTGCGGGTCGCAATCCTGA
CCGGTGAGGGCAAGGCTTTTTCCGCAGGCGGTGACATCAAGGCATGGGGTGGCATGGAGCCGCAAGAATTCGGCCATGCA
TGGGTGCGCTATGGGCATCGTGTGTTCGAGCGGCTGGCGACGTTGCGCGTGCCATTGATCGCGGCCATGAACGGCCATGC
GCTGGGGGGCGGTCTGGAACTGGCGGGAACCGCCGATATCCGCATTGTTGAGCGACAGGCGAAGATCGGCCTGCCGGAAA
CTTCGCTTGGCATGATCCCCGGCTGGTCGGGCACGCAGCGCCTTGTGCGCCGTTTCGGGGCGCAGATCGTGCGCCGCATG
GTGCTGGGCGGCGAAATGTTTACGGCAGATGAAGCCCTGTCGCATGGGCTGGTCGATCATGTGGTCGAGACGGGCGAGGC
TTTGCAGGCCGCGCTGGCCTATGCGGCGCGGGTGGCCAAACGCGGCCCCGCCGCGCTCGAAATTTCCAAACTGATGCTGT
CCGTCGCCAATGGCGAGGACAATGGAACCGCCGTTGAGGCGCTTGGCTCCATTCTTGCCGCCAAGACGGGCGATCTGAAA
GAAGGGGTGCGCTCGTTTACCGAAAAGCGCGAAGCGAATTTTGAAGGAAAATGGTAA

Upstream 100 bases:

>100_bases
GTCTCGGATCAATTGAAGCTCATGGATGAGGCGCTGTTCCGCGAAGAGCCGATCGGGCTTACGCTGCCGCAAAAGCCTGC
GCGAAAGCTGGAGGCGTGAC

Downstream 100 bases:

>100_bases
TGAGCACGATCGTTAAGCCACAGCCGTTGACCGGGCTGGAAGTTCGCGAATTCTCCATGCTGATCGATGGAAAATGGGTG
GGCAGCGACAGCGGAAAGAG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MADRIEISIENLIAVMTIRRPEKLNALDIPLLQELSTACDQVEADSSVRVAILTGEGKAFSAGGDIKAWGGMEPQEFGHA
WVRYGHRVFERLATLRVPLIAAMNGHALGGGLELAGTADIRIVERQAKIGLPETSLGMIPGWSGTQRLVRRFGAQIVRRM
VLGGEMFTADEALSHGLVDHVVETGEALQAALAYAARVAKRGPAALEISKLMLSVANGEDNGTAVEALGSILAAKTGDLK
EGVRSFTEKREANFEGKW

Sequences:

>Translated_258_residues
MADRIEISIENLIAVMTIRRPEKLNALDIPLLQELSTACDQVEADSSVRVAILTGEGKAFSAGGDIKAWGGMEPQEFGHA
WVRYGHRVFERLATLRVPLIAAMNGHALGGGLELAGTADIRIVERQAKIGLPETSLGMIPGWSGTQRLVRRFGAQIVRRM
VLGGEMFTADEALSHGLVDHVVETGEALQAALAYAARVAKRGPAALEISKLMLSVANGEDNGTAVEALGSILAAKTGDLK
EGVRSFTEKREANFEGKW
>Mature_257_residues
ADRIEISIENLIAVMTIRRPEKLNALDIPLLQELSTACDQVEADSSVRVAILTGEGKAFSAGGDIKAWGGMEPQEFGHAW
VRYGHRVFERLATLRVPLIAAMNGHALGGGLELAGTADIRIVERQAKIGLPETSLGMIPGWSGTQRLVRRFGAQIVRRMV
LGGEMFTADEALSHGLVDHVVETGEALQAALAYAARVAKRGPAALEISKLMLSVANGEDNGTAVEALGSILAAKTGDLKE
GVRSFTEKREANFEGKW

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=35.15625, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI4502327, Length=252, Percent_Identity=36.5079365079365, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI68989263, Length=193, Percent_Identity=33.6787564766839, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI20127408, Length=183, Percent_Identity=34.4262295081967, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI31542718, Length=240, Percent_Identity=25.4166666666667, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI70995211, Length=249, Percent_Identity=28.5140562248996, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI157694516, Length=216, Percent_Identity=27.3148148148148, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI213417737, Length=216, Percent_Identity=27.3148148148148, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI62530384, Length=208, Percent_Identity=31.25, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI150378541, Length=253, Percent_Identity=30.0395256916996, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI157694520, Length=174, Percent_Identity=29.3103448275862, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI45643119, Length=218, Percent_Identity=25.2293577981651, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI260275230, Length=218, Percent_Identity=25.2293577981651, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI260274832, Length=218, Percent_Identity=25.2293577981651, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI261878539, Length=95, Percent_Identity=41.0526315789474, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI37594469, Length=186, Percent_Identity=25.2688172043011, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI37594471, Length=186, Percent_Identity=25.2688172043011, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI295842266, Length=206, Percent_Identity=27.6699029126214, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=35.1778656126482, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790281, Length=263, Percent_Identity=31.9391634980989, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI221142681, Length=268, Percent_Identity=33.2089552238806, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788682, Length=197, Percent_Identity=35.0253807106599, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=31.5589353612167, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082183, Length=255, Percent_Identity=27.843137254902, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=28.0155642023346, Blast_Score=81, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI25145438, Length=240, Percent_Identity=36.6666666666667, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=33.6032388663968, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=33.4801762114537, Blast_Score=122, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17558304, Length=175, Percent_Identity=32, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=220, Percent_Identity=25.9090909090909, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=218, Percent_Identity=32.1100917431193, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=218, Percent_Identity=32.1100917431193, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=218, Percent_Identity=32.1100917431193, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=187, Percent_Identity=31.5508021390374, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=247, Percent_Identity=26.3157894736842, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=215, Percent_Identity=26.5116279069767, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=182, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=182, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=197, Percent_Identity=25.8883248730964, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=203, Percent_Identity=26.1083743842365, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=36.9477911646586, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=36.9477911646586, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24653139, Length=268, Percent_Identity=32.089552238806, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24583165, Length=215, Percent_Identity=30.2325581395349, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19921018, Length=213, Percent_Identity=30.9859154929577, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24650670, Length=239, Percent_Identity=30.1255230125523, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21357171, Length=195, Percent_Identity=32.3076923076923, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45550169, Length=247, Percent_Identity=27.1255060728745, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19920382, Length=206, Percent_Identity=27.6699029126214, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=24.5210727969349, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI28571729, Length=172, Percent_Identity=25.5813953488372, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571730, Length=172, Percent_Identity=25.5813953488372, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583077, Length=193, Percent_Identity=33.160621761658, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24583079, Length=193, Percent_Identity=33.160621761658, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19921000, Length=193, Percent_Identity=33.160621761658, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27640; Mature: 27509

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRIEISIENLIAVMTIRRPEKLNALDIPLLQELSTACDQVEADSSVRVAILTGEGKAF
CCCCEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
SAGGDIKAWGGMEPQEFGHAWVRYGHRVFERLATLRVPLIAAMNGHALGGGLELAGTADI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCE
RIVERQAKIGLPETSLGMIPGWSGTQRLVRRFGAQIVRRMVLGGEMFTADEALSHGLVDH
EEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VVETGEALQAALAYAARVAKRGPAALEISKLMLSVANGEDNGTAVEALGSILAAKTGDLK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHH
EGVRSFTEKREANFEGKW
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ADRIEISIENLIAVMTIRRPEKLNALDIPLLQELSTACDQVEADSSVRVAILTGEGKAF
CCCEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
SAGGDIKAWGGMEPQEFGHAWVRYGHRVFERLATLRVPLIAAMNGHALGGGLELAGTADI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCE
RIVERQAKIGLPETSLGMIPGWSGTQRLVRRFGAQIVRRMVLGGEMFTADEALSHGLVDH
EEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VVETGEALQAALAYAARVAKRGPAALEISKLMLSVANGEDNGTAVEALGSILAAKTGDLK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHH
EGVRSFTEKREANFEGKW
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA