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The map label for this gene is dacF [H]

Identifier: 225853470

GI number: 225853470

Start: 1978861

End: 1979973

Strand: Reverse

Name: dacF [H]

Synonym: BMEA_A2096

Alternate gene names: 225853470

Gene position: 1979973-1978861 (Counterclockwise)

Preceding gene: 225853472

Following gene: 225853469

Centisome position: 93.14

GC content: 60.83

Gene sequence:

>1113_bases
ATGTCGAAGTCATTGAGAATCTGGTTTGCGGCAGTGGCCTTTTCCGGTATCGCGTTGTCGGGCGCGGCGGCCAATCCGTC
CATTGCCGTCGATGTGGCCACCGGCAAGGTTTATGAACAGCAGGATGCCTTCCAGCGCTGGTATCCCGCTTCGCTCACCA
AGATGATGACGGCCTATGTCACATTCCGCGCGCTTCAGTCCGGCCAGATGACGCTGGAATCCCCGGTGCGCATGACGGTC
AACGCCTCCAAGGAACCGCCGAGCAAGATGGGGTACAAGCCCGGCTCGATCATGACGCTTGATACGGCGCTGAACATCAT
GCTGGTCAAGTCTGCCAATGATGTTTCGGTGGCCGTTGCCGAAGCCGTGGGCGGAACGGAAGCTGCCTTCGTGCAGCGGA
TGAATGCCGAGGCGCGGCGTCTTGGAATGTTCGGCTCGCATTTCGCCAATCCGAATGGCCTGCATGATCCGAACAATTAT
ACGACAGCGCGCGATCTTGCGGTTCTTGCCGTGCAGTTGCGCCGCGAATTTCCGCAATATGCGCATTATTTCGCGACAGA
AGCGATCGATCCGGGTGCGGGCAAGAAAGTTGAGGCCAATTACAACATCCTGCTTGGCCGCTATGACGGTGCGGATGGCA
TGAAGACGGGCTTCGTCTGCGCATCAGGCTTCAACCTTGCAGGTTCGGCCACCCGCAACGGCCGCACGCTGCTGGCAATC
GTACTGGGTGCGGATCGCCAGGAAACGCGTGCGATCCAGGCCGCGCAAATGATGACCGATGCGTTTCGCGCGCGCAGTTC
CGGCGGGCCGACGCTTGCCACGCTGCGCCCGGCGCAGACAGCCAATCTCAATCAGGCCGTTGATATGCGCAAGGCCATCT
GTAGCGAGCAGGCCATGGCCGACCGCTGGGACGGGCGCGAGGTAGAAGGCCGGCTGAAGATCAATTCACCCTATATTCAC
GCCATGGACCATGATCCGATTGCCGTGCAGGTGAGGCTGGTTTCCGAACCGGGTTCGATCAACCGGCCCGGCCAGCCCGG
CATTTCGCGGGTTCCGGTGCCAATGCCGCGTCCGCAGCGCGCGCCAGGCGTAAAGACAACGGCCATCAACTGA

Upstream 100 bases:

>100_bases
TGCTTATGAATCGTTATCGTGACGATTGCTACGGTTCGCTACGAGGCTGCTGCGATAACCCGCAAAAATTGCGGGCCATT
GTGATTGGAACTGCGGAATG

Downstream 100 bases:

>100_bases
TATGCCTGATCCCATTCCCGTTTCCGTGCTGACCGGCTTTCTTGGCTCCGGCAAGACGACGCTGCTCAACCGGCTTTTGA
AAGATCCGGCGCTCAGCGAT

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MSKSLRIWFAAVAFSGIALSGAAANPSIAVDVATGKVYEQQDAFQRWYPASLTKMMTAYVTFRALQSGQMTLESPVRMTV
NASKEPPSKMGYKPGSIMTLDTALNIMLVKSANDVSVAVAEAVGGTEAAFVQRMNAEARRLGMFGSHFANPNGLHDPNNY
TTARDLAVLAVQLRREFPQYAHYFATEAIDPGAGKKVEANYNILLGRYDGADGMKTGFVCASGFNLAGSATRNGRTLLAI
VLGADRQETRAIQAAQMMTDAFRARSSGGPTLATLRPAQTANLNQAVDMRKAICSEQAMADRWDGREVEGRLKINSPYIH
AMDHDPIAVQVRLVSEPGSINRPGQPGISRVPVPMPRPQRAPGVKTTAIN

Sequences:

>Translated_370_residues
MSKSLRIWFAAVAFSGIALSGAAANPSIAVDVATGKVYEQQDAFQRWYPASLTKMMTAYVTFRALQSGQMTLESPVRMTV
NASKEPPSKMGYKPGSIMTLDTALNIMLVKSANDVSVAVAEAVGGTEAAFVQRMNAEARRLGMFGSHFANPNGLHDPNNY
TTARDLAVLAVQLRREFPQYAHYFATEAIDPGAGKKVEANYNILLGRYDGADGMKTGFVCASGFNLAGSATRNGRTLLAI
VLGADRQETRAIQAAQMMTDAFRARSSGGPTLATLRPAQTANLNQAVDMRKAICSEQAMADRWDGREVEGRLKINSPYIH
AMDHDPIAVQVRLVSEPGSINRPGQPGISRVPVPMPRPQRAPGVKTTAIN
>Mature_369_residues
SKSLRIWFAAVAFSGIALSGAAANPSIAVDVATGKVYEQQDAFQRWYPASLTKMMTAYVTFRALQSGQMTLESPVRMTVN
ASKEPPSKMGYKPGSIMTLDTALNIMLVKSANDVSVAVAEAVGGTEAAFVQRMNAEARRLGMFGSHFANPNGLHDPNNYT
TARDLAVLAVQLRREFPQYAHYFATEAIDPGAGKKVEANYNILLGRYDGADGMKTGFVCASGFNLAGSATRNGRTLLAIV
LGADRQETRAIQAAQMMTDAFRARSSGGPTLATLRPAQTANLNQAVDMRKAICSEQAMADRWDGREVEGRLKINSPYIHA
MDHDPIAVQVRLVSEPGSINRPGQPGISRVPVPMPRPQRAPGVKTTAIN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=243, Percent_Identity=32.9218106995885, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI87082022, Length=241, Percent_Identity=31.5352697095436, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786851, Length=257, Percent_Identity=32.6848249027237, Blast_Score=114, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 39822; Mature: 39691

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSLRIWFAAVAFSGIALSGAAANPSIAVDVATGKVYEQQDAFQRWYPASLTKMMTAYV
CCCCEEEEEEEEHHCCEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHH
TFRALQSGQMTLESPVRMTVNASKEPPSKMGYKPGSIMTLDTALNIMLVKSANDVSVAVA
HHHHHHCCCEEECCCEEEEEECCCCCCHHHCCCCCCEEEEECCEEEEEEECCCCCEEEEE
EAVGGTEAAFVQRMNAEARRLGMFGSHFANPNGLHDPNNYTTARDLAVLAVQLRREFPQY
HHCCCCHHHHHHHHCHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AHYFATEAIDPGAGKKVEANYNILLGRYDGADGMKTGFVCASGFNLAGSATRNGRTLLAI
HHHHHHHCCCCCCCCEEECCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCEEEEE
VLGADRQETRAIQAAQMMTDAFRARSSGGPTLATLRPAQTANLNQAVDMRKAICSEQAMA
EECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
DRWDGREVEGRLKINSPYIHAMDHDPIAVQVRLVSEPGSINRPGQPGISRVPVPMPRPQR
HCCCCCEEEEEEEECCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
APGVKTTAIN
CCCCEEECCC
>Mature Secondary Structure 
SKSLRIWFAAVAFSGIALSGAAANPSIAVDVATGKVYEQQDAFQRWYPASLTKMMTAYV
CCCEEEEEEEEHHCCEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHH
TFRALQSGQMTLESPVRMTVNASKEPPSKMGYKPGSIMTLDTALNIMLVKSANDVSVAVA
HHHHHHCCCEEECCCEEEEEECCCCCCHHHCCCCCCEEEEECCEEEEEEECCCCCEEEEE
EAVGGTEAAFVQRMNAEARRLGMFGSHFANPNGLHDPNNYTTARDLAVLAVQLRREFPQY
HHCCCCHHHHHHHHCHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AHYFATEAIDPGAGKKVEANYNILLGRYDGADGMKTGFVCASGFNLAGSATRNGRTLLAI
HHHHHHHCCCCCCCCEEECCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCEEEEE
VLGADRQETRAIQAAQMMTDAFRARSSGGPTLATLRPAQTANLNQAVDMRKAICSEQAMA
EECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
DRWDGREVEGRLKINSPYIHAMDHDPIAVQVRLVSEPGSINRPGQPGISRVPVPMPRPQR
HCCCCCEEEEEEEECCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
APGVKTTAIN
CCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]