Click here to switch to the map view.

The map label for this gene is ansA [H]

Identifier: 225853396

GI number: 225853396

Start: 1908017

End: 1909015

Strand: Direct

Name: ansA [H]

Synonym: BMEA_A2017

Alternate gene names: 225853396

Gene position: 1908017-1909015 (Clockwise)

Preceding gene: 225853395

Following gene: 225853399

Centisome position: 89.76

GC content: 58.76

Gene sequence:

>999_bases
ATGAAGACAGGCAGGACGGATCGCCCCAGAATTCTCATCGTTTCCACCGGCGGAACCATCGCCTCGCGTTACGATCCGCA
AACGGATTCGCTGTTGAGCGTTTCGACCGGAAAGGAACTTCTATCCTCTCTCGGTGCGCTTGCCCCTGCAGTCGATGTTG
TTCTTGACGAATTCTGCAATCTTGGCAGCAACCGCATCGATCTCGATACATCTTTCAGGCTTGCGCATCATATTGATGAA
CGGCTCAGGGACCGAACCGTCGACGGGTGTGTGGTCACACACGGCACGGACACGCTGGAGGAAAGCGCATTCCTCGCACA
GCTTACGATATCCTCGCGAAAGCCGGTGGTCTTTACCGGAGCCCAGCGCAGCGCAGACGAACCGGATGCCGACGGACCAC
GCAATCTTGCCGACGCAATCTCCGTTGCCGCCTCGCCCGAGGCTCGCGATCTCGGCACGCTCGTCGTCTTCGCCGGGAAG
GTGCTCTCCTCTATCGACGCGACAAAAATTCATACGTCGAAGCTCGAAGCCTATGATGCCCCCGGATATGGGCCGCTCGG
CGTGGTGGATCACGGCGCGGTATTCATCACAAGAAAACCGCTGGGCTATCCGATGATTGCCGCCCATTCGATCGAAACTC
GGGTCGATCTCATCACACTCGCGATGGGTGCCGATGGCCGTCTCATCGAAGCGGCGATCAATTCCGGCACACACGGCTTC
ATTCTGGAAACATTCGGCCGGGGCAACGCCACGCCCGCAGTTGTCGATGCCATCGCCCGAGCAACGGAACGGGGCATCGT
AACAATCATCACCTCGCGCTGCCCGGATGGCCGGGTGCTGCCTGTCTACGGCGATGGAGGCGGAAGCGACCTTGAAAAGG
CCGGAGCAATCTTCGCCGGTCGGCTGAACGGCCCCAAAGCACGAATTCTCCTTTGTCTGTCATTGCCAACCATCCCTGTT
GAGGAGCTAGGCCGCCTTTTTGAGCAGTTTGGACGATAA

Upstream 100 bases:

>100_bases
GCAACATCATTGAAAGCGCGGAACCATCCATATTCTTCGACGCGCCGAAACATGAACGGACCCGGCTCTTTCTCAGCCAG
ATATTGCATTAGGCTCCGGC

Downstream 100 bases:

>100_bases
AAAGGAAAACGGGTTGAGCGTATCGAGGGACGGGAGGGGCCAAAAGTGCCGAACAAGCAACCGAAACGCAGCCCTTTGTT
GCTGAAAGACAGCCGTGTAT

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKTGRTDRPRILIVSTGGTIASRYDPQTDSLLSVSTGKELLSSLGALAPAVDVVLDEFCNLGSNRIDLDTSFRLAHHIDE
RLRDRTVDGCVVTHGTDTLEESAFLAQLTISSRKPVVFTGAQRSADEPDADGPRNLADAISVAASPEARDLGTLVVFAGK
VLSSIDATKIHTSKLEAYDAPGYGPLGVVDHGAVFITRKPLGYPMIAAHSIETRVDLITLAMGADGRLIEAAINSGTHGF
ILETFGRGNATPAVVDAIARATERGIVTIITSRCPDGRVLPVYGDGGGSDLEKAGAIFAGRLNGPKARILLCLSLPTIPV
EELGRLFEQFGR

Sequences:

>Translated_332_residues
MKTGRTDRPRILIVSTGGTIASRYDPQTDSLLSVSTGKELLSSLGALAPAVDVVLDEFCNLGSNRIDLDTSFRLAHHIDE
RLRDRTVDGCVVTHGTDTLEESAFLAQLTISSRKPVVFTGAQRSADEPDADGPRNLADAISVAASPEARDLGTLVVFAGK
VLSSIDATKIHTSKLEAYDAPGYGPLGVVDHGAVFITRKPLGYPMIAAHSIETRVDLITLAMGADGRLIEAAINSGTHGF
ILETFGRGNATPAVVDAIARATERGIVTIITSRCPDGRVLPVYGDGGGSDLEKAGAIFAGRLNGPKARILLCLSLPTIPV
EELGRLFEQFGR
>Mature_332_residues
MKTGRTDRPRILIVSTGGTIASRYDPQTDSLLSVSTGKELLSSLGALAPAVDVVLDEFCNLGSNRIDLDTSFRLAHHIDE
RLRDRTVDGCVVTHGTDTLEESAFLAQLTISSRKPVVFTGAQRSADEPDADGPRNLADAISVAASPEARDLGTLVVFAGK
VLSSIDATKIHTSKLEAYDAPGYGPLGVVDHGAVFITRKPLGYPMIAAHSIETRVDLITLAMGADGRLIEAAINSGTHGF
ILETFGRGNATPAVVDAIARATERGIVTIITSRCPDGRVLPVYGDGGGSDLEKAGAIFAGRLNGPKARILLCLSLPTIPV
EELGRLFEQFGR

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=205, Percent_Identity=30.2439024390244, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1789327, Length=336, Percent_Identity=32.4404761904762, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1788065, Length=308, Percent_Identity=25.974025974026, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI193207372, Length=269, Percent_Identity=27.8810408921933, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17558278, Length=269, Percent_Identity=27.8810408921933, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17558280, Length=269, Percent_Identity=27.8810408921933, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI193207374, Length=269, Percent_Identity=27.8810408921933, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323189, Length=320, Percent_Identity=34.0625, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6323187, Length=320, Percent_Identity=34.0625, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6323186, Length=320, Percent_Identity=34.0625, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6323184, Length=320, Percent_Identity=34.0625, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6320527, Length=338, Percent_Identity=30.1775147928994, Blast_Score=116, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24645530, Length=246, Percent_Identity=31.7073170731707, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24639609, Length=241, Percent_Identity=26.9709543568465, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35070; Mature: 35070

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTGRTDRPRILIVSTGGTIASRYDPQTDSLLSVSTGKELLSSLGALAPAVDVVLDEFCN
CCCCCCCCCEEEEEECCCCEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
LGSNRIDLDTSFRLAHHIDERLRDRTVDGCVVTHGTDTLEESAFLAQLTISSRKPVVFTG
CCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHEEEEEEECCCCCEEEEC
AQRSADEPDADGPRNLADAISVAASPEARDLGTLVVFAGKVLSSIDATKIHTSKLEAYDA
CCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHEEHHHHHHCCC
PGYGPLGVVDHGAVFITRKPLGYPMIAAHSIETRVDLITLAMGADGRLIEAAINSGTHGF
CCCCCCCEECCCEEEEEECCCCCCEEECCCHHHHEEEEEEEECCCCCEEEEECCCCCCEE
ILETFGRGNATPAVVDAIARATERGIVTIITSRCPDGRVLPVYGDGGGSDLEKAGAIFAG
EEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEECCCCCCHHHHCCEEEE
RLNGPKARILLCLSLPTIPVEELGRLFEQFGR
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKTGRTDRPRILIVSTGGTIASRYDPQTDSLLSVSTGKELLSSLGALAPAVDVVLDEFCN
CCCCCCCCCEEEEEECCCCEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
LGSNRIDLDTSFRLAHHIDERLRDRTVDGCVVTHGTDTLEESAFLAQLTISSRKPVVFTG
CCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHEEEEEEECCCCCEEEEC
AQRSADEPDADGPRNLADAISVAASPEARDLGTLVVFAGKVLSSIDATKIHTSKLEAYDA
CCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHEEHHHHHHCCC
PGYGPLGVVDHGAVFITRKPLGYPMIAAHSIETRVDLITLAMGADGRLIEAAINSGTHGF
CCCCCCCEECCCEEEEEECCCCCCEEECCCHHHHEEEEEEEECCCCCEEEEECCCCCCEE
ILETFGRGNATPAVVDAIARATERGIVTIITSRCPDGRVLPVYGDGGGSDLEKAGAIFAG
EEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEECCCCCCHHHHCCEEEE
RLNGPKARILLCLSLPTIPVEELGRLFEQFGR
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]