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The map label for this gene is dapF

Identifier: 225853370

GI number: 225853370

Start: 1882201

End: 1883112

Strand: Direct

Name: dapF

Synonym: BMEA_A1989

Alternate gene names: 225853370

Gene position: 1882201-1883112 (Clockwise)

Preceding gene: 225853362

Following gene: 225853371

Centisome position: 88.54

GC content: 63.27

Gene sequence:

>912_bases
ATGGCCACAAAGGCAGCCTTCGCCAGGATGAACGGGCTCGGCAACCAGATCATCGTTGCCGATATGCGCGGGCGCGCGGA
TAGCATCACCTCCGCCGCAGCCATCCGTCTGGCAAGCGATAGCGAAACAGCCTTCGATCAGATCATGGCGATCCACGATC
CGCGTACGCCCGGCACGGACTATTACATAGCGATCATCAATTGCGACGGCACGCAAGCACAGGCTTGCGGCAACGGCACG
CGCTGTGTGGTGCAGGCGCTCGCCGCCGAGACCGGCAGGCACGCCTTTACTTTCGAGACCCGCGCCGGAATCCTGACCGC
GACCGAACATGACGACGGGCTGATTTCCGTCGATATGGGCACGCCGCGCTTCGACTGGCAGGACATTCCGCTTGCCCAAG
CCGTCGCCGATACGCGTAAAATCGAATTGCAGGTCGGCCCGGCGGATGCGCCTGTCCTGCATTCCCCTTCCATCGCCTCC
ATGGGCAACCCCCATGCGGTCTTCTGGGTGGATAAGGATGTCTGGTCCTATGAGCTGGACAAGTTCGGGCCGCTTCTCGA
AAACCATCCGATCTTCCCCGAACGCGCCAATATTTCCATCGCCCATGTCACTTCATCCGACACGATAGACCTGCGCACAT
GGGAGCGCGGCGCAGGACTGACCCGTGCCTGCGGGTCGGCAGCCTGTGCTGCTGCCGTTTCAGCCGCCCGCACGGGGCGC
ACCGGCCGCAAGGTCACGGTCAATGTTCCCGGCGGACCGCTCCTCATCGAATGGCGCGACGATGACCATGTGATGATGAC
CGGCCCCGCCGAATGGGAATTCTCCGGCACCTTCGATCCTGCAACCGGCGAATGGAGCCGCGACACGCAGGGTTTGCAGG
GTTCCGGCAACGCCGACAGGGGGGCTGCCTGA

Upstream 100 bases:

>100_bases
CGCGCGCGGGTACTGGCAATTTGCGGGCTTTTCCGCCATATAGACCCTCATTGTCAGGCTTGATGACCAAAGCCGCAGGA
AGAATGGAACCACGTTAGTT

Downstream 100 bases:

>100_bases
TGTCTGTGGAAATCGTCACCTTCGGATGCCGCCTCAACACCTATGAATCGGAAGTGATGAGGCGCGAAGCCGACGCTGCC
GGGCTGGGCACGCTGAAAGA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MATKAAFARMNGLGNQIIVADMRGRADSITSAAAIRLASDSETAFDQIMAIHDPRTPGTDYYIAIINCDGTQAQACGNGT
RCVVQALAAETGRHAFTFETRAGILTATEHDDGLISVDMGTPRFDWQDIPLAQAVADTRKIELQVGPADAPVLHSPSIAS
MGNPHAVFWVDKDVWSYELDKFGPLLENHPIFPERANISIAHVTSSDTIDLRTWERGAGLTRACGSAACAAAVSAARTGR
TGRKVTVNVPGGPLLIEWRDDDHVMMTGPAEWEFSGTFDPATGEWSRDTQGLQGSGNADRGAA

Sequences:

>Translated_303_residues
MATKAAFARMNGLGNQIIVADMRGRADSITSAAAIRLASDSETAFDQIMAIHDPRTPGTDYYIAIINCDGTQAQACGNGT
RCVVQALAAETGRHAFTFETRAGILTATEHDDGLISVDMGTPRFDWQDIPLAQAVADTRKIELQVGPADAPVLHSPSIAS
MGNPHAVFWVDKDVWSYELDKFGPLLENHPIFPERANISIAHVTSSDTIDLRTWERGAGLTRACGSAACAAAVSAARTGR
TGRKVTVNVPGGPLLIEWRDDDHVMMTGPAEWEFSGTFDPATGEWSRDTQGLQGSGNADRGAA
>Mature_302_residues
ATKAAFARMNGLGNQIIVADMRGRADSITSAAAIRLASDSETAFDQIMAIHDPRTPGTDYYIAIINCDGTQAQACGNGTR
CVVQALAAETGRHAFTFETRAGILTATEHDDGLISVDMGTPRFDWQDIPLAQAVADTRKIELQVGPADAPVLHSPSIASM
GNPHAVFWVDKDVWSYELDKFGPLLENHPIFPERANISIAHVTSSDTIDLRTWERGAGLTRACGSAACAAAVSAARTGRT
GRKVTVNVPGGPLLIEWRDDDHVMMTGPAEWEFSGTFDPATGEWSRDTQGLQGSGNADRGAA

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=275, Percent_Identity=37.4545454545455, Blast_Score=172, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_BRUA1 (B2S885)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935768.1
- ProteinModelPortal:   B2S885
- SMR:   B2S885
- GeneID:   6326912
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I18120
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 32310; Mature: 32179

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 76-76 ACT_SITE 224-224

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKAAFARMNGLGNQIIVADMRGRADSITSAAAIRLASDSETAFDQIMAIHDPRTPGTD
CCCHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHEEEECCCCCCCC
YYIAIINCDGTQAQACGNGTRCVVQALAAETGRHAFTFETRAGILTATEHDDGLISVDMG
EEEEEEECCCCCHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCEEEEECC
TPRFDWQDIPLAQAVADTRKIELQVGPADAPVLHSPSIASMGNPHAVFWVDKDVWSYELD
CCCCCCCCCCHHHHHCCCEEEEEEECCCCCCEEECCCHHCCCCCEEEEEEECCCCEEEHH
KFGPLLENHPIFPERANISIAHVTSSDTIDLRTWERGAGLTRACGSAACAAAVSAARTGR
HHCCHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
TGRKVTVNVPGGPLLIEWRDDDHVMMTGPAEWEFSGTFDPATGEWSRDTQGLQGSGNADR
CCCEEEEECCCCEEEEEEECCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
GAA
CCC
>Mature Secondary Structure 
ATKAAFARMNGLGNQIIVADMRGRADSITSAAAIRLASDSETAFDQIMAIHDPRTPGTD
CCHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHEEEECCCCCCCC
YYIAIINCDGTQAQACGNGTRCVVQALAAETGRHAFTFETRAGILTATEHDDGLISVDMG
EEEEEEECCCCCHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCEEEEECC
TPRFDWQDIPLAQAVADTRKIELQVGPADAPVLHSPSIASMGNPHAVFWVDKDVWSYELD
CCCCCCCCCCHHHHHCCCEEEEEEECCCCCCEEECCCHHCCCCCEEEEEEECCCCEEEHH
KFGPLLENHPIFPERANISIAHVTSSDTIDLRTWERGAGLTRACGSAACAAAVSAARTGR
HHCCHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
TGRKVTVNVPGGPLLIEWRDDDHVMMTGPAEWEFSGTFDPATGEWSRDTQGLQGSGNADR
CCCEEEEECCCCEEEEEEECCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
GAA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA