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The map label for this gene is gltX [H]

Identifier: 225853112

GI number: 225853112

Start: 1615924

End: 1616799

Strand: Direct

Name: gltX [H]

Synonym: BMEA_A1702

Alternate gene names: 225853112

Gene position: 1615924-1616799 (Clockwise)

Preceding gene: 225853109

Following gene: 225853113

Centisome position: 76.02

GC content: 58.56

Gene sequence:

>876_bases
ATGACCGTACCTGTTTTCCGCTTTGCTCCAAGTCCCAATGGCCAGCTCCATCTGGGCCATGCCTATTCCGCGCTTTTGAA
TGCGAACATGGCCGAACAAAGCGGCGGACGCTTCCTGCTCCGCATGGAAGATATCGATACCGCGCGCTGCACACCGGCAC
TGGAACAAGGGATTTACGACGATCTCCACTGGCTTGGGCTAAGATGGGAAACCCCGGTGCGCCGCCAGTCGGAACATTTT
GGCGAATATCGCAACGCCCTTACTAAACTTGCCGACAAGGGCCTTGTCTATCCCGCGTTCCTCAGCCGGAGCGAGGTTCG
CAAGCGCATCAGCGAAGCTTGCGCCAGAGGGGATGACTGGCCTTCAGACCCGGACGGCACGCCGCTTTATCCGCAAGACG
AACGCAACATGAGCGAAAAAGAGCAGATGGAGCACATCGTTTCGGGCGCGCCCTATGCCTGGCGGCTCGACATGGATAAG
GCGCTTGCCTCTGCGGGCGAACCGCTATTCTGGAACGAAAGCGGTGCCGGACCCGATGGCGAAACAGGTCGCCTTGCTGC
CGACCCCGCCAGATGGGGCGATGTGGTGATTGCGCGCAAGGACACACCGACCAGCTATCACCTTTCCGTCGTGGTGGATG
ATGCGTTGCAGGGCATCACCCATATCGTGCGCGGGCGCGATCTTTTTCATGCAACATCGGTGCACCGGCTTTTGCAGAAA
CTTCTGGGCCTGCCAGAGCCGCTTTATCACCATCACGATCTTGTTCTGGGCGAAGACGGACAGAAACTTTCCAAAAGCCG
CAAGGATACGGCTCTGGCATCGCTGCGTGAACAGGGTTGGACGCCCGCCGATATTCGCGCCAAACTCGGTTTATAG

Upstream 100 bases:

>100_bases
CGCTCAATGTATCGATCCGCCGCATCGCCTGGCCTTTCATTCTCCCGGCACACAACGTACCAATGGAATCAAATGACATT
CAACTGACATTGAACCCGCA

Downstream 100 bases:

>100_bases
CCGCCATTTTCGCCTCAGCGTCACAAATGATGTGCCCGCGCACCGATTGACGAAAACGCGCCAAGGCGGTTCAAAGGCAG
TATGATGACCGGCATTTAAA

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTVPVFRFAPSPNGQLHLGHAYSALLNANMAEQSGGRFLLRMEDIDTARCTPALEQGIYDDLHWLGLRWETPVRRQSEHF
GEYRNALTKLADKGLVYPAFLSRSEVRKRISEACARGDDWPSDPDGTPLYPQDERNMSEKEQMEHIVSGAPYAWRLDMDK
ALASAGEPLFWNESGAGPDGETGRLAADPARWGDVVIARKDTPTSYHLSVVVDDALQGITHIVRGRDLFHATSVHRLLQK
LLGLPEPLYHHHDLVLGEDGQKLSKSRKDTALASLREQGWTPADIRAKLGL

Sequences:

>Translated_291_residues
MTVPVFRFAPSPNGQLHLGHAYSALLNANMAEQSGGRFLLRMEDIDTARCTPALEQGIYDDLHWLGLRWETPVRRQSEHF
GEYRNALTKLADKGLVYPAFLSRSEVRKRISEACARGDDWPSDPDGTPLYPQDERNMSEKEQMEHIVSGAPYAWRLDMDK
ALASAGEPLFWNESGAGPDGETGRLAADPARWGDVVIARKDTPTSYHLSVVVDDALQGITHIVRGRDLFHATSVHRLLQK
LLGLPEPLYHHHDLVLGEDGQKLSKSRKDTALASLREQGWTPADIRAKLGL
>Mature_290_residues
TVPVFRFAPSPNGQLHLGHAYSALLNANMAEQSGGRFLLRMEDIDTARCTPALEQGIYDDLHWLGLRWETPVRRQSEHFG
EYRNALTKLADKGLVYPAFLSRSEVRKRISEACARGDDWPSDPDGTPLYPQDERNMSEKEQMEHIVSGAPYAWRLDMDKA
LASAGEPLFWNESGAGPDGETGRLAADPARWGDVVIARKDTPTSYHLSVVVDDALQGITHIVRGRDLFHATSVHRLLQKL
LGLPEPLYHHHDLVLGEDGQKLSKSRKDTALASLREQGWTPADIRAKLGL

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=285, Percent_Identity=32.280701754386, Blast_Score=130, Evalue=1e-30,
Organism=Escherichia coli, GI1788743, Length=276, Percent_Identity=30.7971014492754, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI226510925, Length=266, Percent_Identity=32.7067669172932, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786895, Length=218, Percent_Identity=25.6880733944954, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71993394, Length=280, Percent_Identity=28.5714285714286, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324540, Length=277, Percent_Identity=30.3249097472924, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321192, Length=220, Percent_Identity=24.0909090909091, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24665321, Length=285, Percent_Identity=32.6315789473684, Blast_Score=124, Evalue=7e-29,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020752
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 32479; Mature: 32348

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPVFRFAPSPNGQLHLGHAYSALLNANMAEQSGGRFLLRMEDIDTARCTPALEQGIYD
CCCCEEEECCCCCCCEEEHHHHHHHHHCCHHHCCCCEEEEEECCCCCHHCCHHHHHHHHH
DLHWLGLRWETPVRRQSEHFGEYRNALTKLADKGLVYPAFLSRSEVRKRISEACARGDDW
HHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHHHCCCCC
PSDPDGTPLYPQDERNMSEKEQMEHIVSGAPYAWRLDMDKALASAGEPLFWNESGAGPDG
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCEEECCCCCCCCC
ETGRLAADPARWGDVVIARKDTPTSYHLSVVVDDALQGITHIVRGRDLFHATSVHRLLQK
CCCCEECCCCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LLGLPEPLYHHHDLVLGEDGQKLSKSRKDTALASLREQGWTPADIRAKLGL
HHCCCCCHHHCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC
>Mature Secondary Structure 
TVPVFRFAPSPNGQLHLGHAYSALLNANMAEQSGGRFLLRMEDIDTARCTPALEQGIYD
CCCEEEECCCCCCCEEEHHHHHHHHHCCHHHCCCCEEEEEECCCCCHHCCHHHHHHHHH
DLHWLGLRWETPVRRQSEHFGEYRNALTKLADKGLVYPAFLSRSEVRKRISEACARGDDW
HHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHHHCCCCC
PSDPDGTPLYPQDERNMSEKEQMEHIVSGAPYAWRLDMDKALASAGEPLFWNESGAGPDG
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCEEECCCCCCCCC
ETGRLAADPARWGDVVIARKDTPTSYHLSVVVDDALQGITHIVRGRDLFHATSVHRLLQK
CCCCEECCCCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LLGLPEPLYHHHDLVLGEDGQKLSKSRKDTALASLREQGWTPADIRAKLGL
HHCCCCCHHHCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]