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The map label for this gene is mltB [H]

Identifier: 225852929

GI number: 225852929

Start: 1426361

End: 1427602

Strand: Direct

Name: mltB [H]

Synonym: BMEA_A1498

Alternate gene names: 225852929

Gene position: 1426361-1427602 (Clockwise)

Preceding gene: 225852928

Following gene: 225852930

Centisome position: 67.1

GC content: 58.29

Gene sequence:

>1242_bases
TTGTTTTCCATGAAGATTATCGCCTCAGTTCTGGCTGCGGCCTTGTTTGCCTCCACGGCTCTATCGGGAGCCGGGGCAGC
GACAACCCCGGCTCCCGCAGCCGCGACAGATAATGCCGTCAACATGCCGAAGCCGGAATGTGAAACTGACTTCGGTCAAT
GGATGGAAAATCTGACCAGGGAAGCCCGTGAAGCCGGCGTCGGTGAAAAAGGCATTACCGAATTGCACAAGGCTTCCATC
GACCAGAAAGTTCTGGGCCGCGACCGCAAGCAGACCGTTTTCAATCTCACTTTCACCGAATTTTCCAAGCGCCTCATTTC
CGAGGCACGCCTGAAGAAAGGCCAGGAAAATCTCGTCAAATATGCCGATGTCTTCAAGAAAGTTGAAGATGCTTATGGCG
TTCCGGGTCCGGTTCTTGCCGCTTTCTGGGGGCTTGAAACCGACTATGGCGCGATTCAGGGCGATTTCGACACGCTCAAT
GCGCTCGTCACGCTCTCCTACGACTGCCGCCGCCCGGACCTGTTCCGTCCGCAGCTCATCGCCCTGCTCAAGCTCTTCGA
TCAGGGCGTGGTCGATGCAAATACGACCGGCGCCTGGGCAGGCGAAATCGGCATGATGCAGCTTCTGCCGAAAGACTATC
TGGAACGCGGCGTCGATGGCGACGGCGATGGCAAGGTCGATCTGAAGAACAGCGTGCCCGATGCCATGATGACCGCAGGG
CGCATGCTTTCGGAACTGGGCTGGAAGCGCGGCCAGCCATGGCTTGAAGAAGTGCGCCTCACCAAGGACCTCCCCTGGGA
AGAGGCGATCCGCACCAACCGCAAGCCGCATTCATGGTGGGCTGAGCATGGCGTGACGGGCCTCAATGGCCCACTCGGCC
CTGATGATGGCGATGCATCCCTTCTGCTGCCGCTTGGCCGCAAGGGACCGGCTTTCCTGTCCTATCTGAATTTTGACATC
TTCGTCGAATGGAACAAATCCATAGTCTATGCGACGACTGCCGCCTATTTTGCAACCCGCCTTGCAGGTGCCCCTGCTTT
CGATCCGGGCACCCCGGTTCCGGGGCTGACGCAGGATCAACTCAAGGAATTGCAGACCAAGCTTCAGGCGCGCGGTTATG
AAATGGGCAAGATCGATGGTGTTTTCGGTGTCGCGACCCGCGATGCGGTACGCTCCGAACAGTTGCGCCTTGGCATGCCC
GCCGATTCCTGGCCGACACAGGAACTTCTCGACAGGCTTTGA

Upstream 100 bases:

>100_bases
TCGACCGCTCACATTAAGTTTTGCTTCATTTCTTTGCTTTGATGGGGCAATAGCTAACCAAACCGTAAAGGGGGGCCAGC
GCCACCCAACTTAGCGTGAG

Downstream 100 bases:

>100_bases
GGTTGTCTCGCAGGCGCAAGGCACCCTTGCGCCTTTCCTTCCCTGAATTGAATGCCACACCATGCTTACAACGCTGATTG
TCGTTCTCCCCGTCTTTGCG

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MFSMKIIASVLAAALFASTALSGAGAATTPAPAAATDNAVNMPKPECETDFGQWMENLTREAREAGVGEKGITELHKASI
DQKVLGRDRKQTVFNLTFTEFSKRLISEARLKKGQENLVKYADVFKKVEDAYGVPGPVLAAFWGLETDYGAIQGDFDTLN
ALVTLSYDCRRPDLFRPQLIALLKLFDQGVVDANTTGAWAGEIGMMQLLPKDYLERGVDGDGDGKVDLKNSVPDAMMTAG
RMLSELGWKRGQPWLEEVRLTKDLPWEEAIRTNRKPHSWWAEHGVTGLNGPLGPDDGDASLLLPLGRKGPAFLSYLNFDI
FVEWNKSIVYATTAAYFATRLAGAPAFDPGTPVPGLTQDQLKELQTKLQARGYEMGKIDGVFGVATRDAVRSEQLRLGMP
ADSWPTQELLDRL

Sequences:

>Translated_413_residues
MFSMKIIASVLAAALFASTALSGAGAATTPAPAAATDNAVNMPKPECETDFGQWMENLTREAREAGVGEKGITELHKASI
DQKVLGRDRKQTVFNLTFTEFSKRLISEARLKKGQENLVKYADVFKKVEDAYGVPGPVLAAFWGLETDYGAIQGDFDTLN
ALVTLSYDCRRPDLFRPQLIALLKLFDQGVVDANTTGAWAGEIGMMQLLPKDYLERGVDGDGDGKVDLKNSVPDAMMTAG
RMLSELGWKRGQPWLEEVRLTKDLPWEEAIRTNRKPHSWWAEHGVTGLNGPLGPDDGDASLLLPLGRKGPAFLSYLNFDI
FVEWNKSIVYATTAAYFATRLAGAPAFDPGTPVPGLTQDQLKELQTKLQARGYEMGKIDGVFGVATRDAVRSEQLRLGMP
ADSWPTQELLDRL
>Mature_413_residues
MFSMKIIASVLAAALFASTALSGAGAATTPAPAAATDNAVNMPKPECETDFGQWMENLTREAREAGVGEKGITELHKASI
DQKVLGRDRKQTVFNLTFTEFSKRLISEARLKKGQENLVKYADVFKKVEDAYGVPGPVLAAFWGLETDYGAIQGDFDTLN
ALVTLSYDCRRPDLFRPQLIALLKLFDQGVVDANTTGAWAGEIGMMQLLPKDYLERGVDGDGDGKVDLKNSVPDAMMTAG
RMLSELGWKRGQPWLEEVRLTKDLPWEEAIRTNRKPHSWWAEHGVTGLNGPLGPDDGDASLLLPLGRKGPAFLSYLNFDI
FVEWNKSIVYATTAAYFATRLAGAPAFDPGTPVPGLTQDQLKELQTKLQARGYEMGKIDGVFGVATRDAVRSEQLRLGMP
ADSWPTQELLDRL

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=159, Percent_Identity=30.188679245283, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 45232; Mature: 45232

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSMKIIASVLAAALFASTALSGAGAATTPAPAAATDNAVNMPKPECETDFGQWMENLTR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
EAREAGVGEKGITELHKASIDQKVLGRDRKQTVFNLTFTEFSKRLISEARLKKGQENLVK
HHHHCCCCHHHHHHHHHHHCCHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHCCHHHHHH
YADVFKKVEDAYGVPGPVLAAFWGLETDYGAIQGDFDTLNALVTLSYDCRRPDLFRPQLI
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHEEEECCCCCCCCHHHHH
ALLKLFDQGVVDANTTGAWAGEIGMMQLLPKDYLERGVDGDGDGKVDLKNSVPDAMMTAG
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCCEEECCCCCHHHHHHH
RMLSELGWKRGQPWLEEVRLTKDLPWEEAIRTNRKPHSWWAEHGVTGLNGPLGPDDGDAS
HHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCE
LLLPLGRKGPAFLSYLNFDIFVEWNKSIVYATTAAYFATRLAGAPAFDPGTPVPGLTQDQ
EEEECCCCCCHHHHHHCEEEEEEECCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
LKELQTKLQARGYEMGKIDGVFGVATRDAVRSEQLRLGMPADSWPTQELLDRL
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHCC
>Mature Secondary Structure
MFSMKIIASVLAAALFASTALSGAGAATTPAPAAATDNAVNMPKPECETDFGQWMENLTR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
EAREAGVGEKGITELHKASIDQKVLGRDRKQTVFNLTFTEFSKRLISEARLKKGQENLVK
HHHHCCCCHHHHHHHHHHHCCHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHCCHHHHHH
YADVFKKVEDAYGVPGPVLAAFWGLETDYGAIQGDFDTLNALVTLSYDCRRPDLFRPQLI
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHEEEECCCCCCCCHHHHH
ALLKLFDQGVVDANTTGAWAGEIGMMQLLPKDYLERGVDGDGDGKVDLKNSVPDAMMTAG
HHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCCEEECCCCCHHHHHHH
RMLSELGWKRGQPWLEEVRLTKDLPWEEAIRTNRKPHSWWAEHGVTGLNGPLGPDDGDAS
HHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCE
LLLPLGRKGPAFLSYLNFDIFVEWNKSIVYATTAAYFATRLAGAPAFDPGTPVPGLTQDQ
EEEECCCCCCHHHHHHCEEEEEEECCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
LKELQTKLQARGYEMGKIDGVFGVATRDAVRSEQLRLGMPADSWPTQELLDRL
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]