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The map label for this gene is sseA [H]

Identifier: 225852558

GI number: 225852558

Start: 1043219

End: 1044073

Strand: Direct

Name: sseA [H]

Synonym: BMEA_A1096

Alternate gene names: 225852558

Gene position: 1043219-1044073 (Clockwise)

Preceding gene: 225852557

Following gene: 225852559

Centisome position: 49.08

GC content: 58.25

Gene sequence:

>855_bases
ATGACCGAGAAAAGCGCATTCGTCGTATCGCGCGACTGGTTGAAGGAACGTCTGCATAAGCCCGGCCTCGCGATCGTCGA
TGCCTCCTGGTATTTGCCTGCGGCAGGGCGCAACGGTCAGGAGGAATATGAAAAAGCGCATATTCCGGGCGCGGTATTCT
TCGATCAGGATAAAATCGCCGACAAGGAGTCCGGCCTTCCGCATACCCTGCCATCGCCGGAATTCTTCGCGCAGCAAGTA
GGCACGCTGGGCATCACTGCCGACGAAACCGTGGTGGTTTATGATGGCCCCGGCATGTTTTCCGCCCCGCGCGTCTGGTG
GATGTTCCGCGTCATGGGCGTGAAGAATGTCTATGTTCTCGATGGCGGATTCGACGGCTGGAAAAAGGCGGGCTACCCCG
TCACTGACGAAGTAACGAAGATTGCCGCGACATTTTTTAAGCCCTCCTTCAACAAGGACGCAGTTGTCGATTTTCAGGAA
ATGCGCAAGATCGTTGATGAAAAACGCTCGCAGATTGCCGATGCGCGTGGCGCGGGCCGTTTTACGGGGCGCGACGCGGA
ACCTCGCGCGGGAATGCGCTCGGGCCATATGCCGGGTGCGCGCAATGTTCCTGTTACAACCCTTTCCGAAAACGGTGAAT
TGAAAGACCTCGAAAGCCTGCGCAGGATTTTTGACGAGGCGGGTATCGACCTGTCGGGGCCGGTGGTCACCAGTTGCGGT
TCCGGTGTTACCGCTGCCGTGATTACGCTCGCGCTTACCTCGCTGGGGCACAAGGATAACCGCCTCTATGACGGTTCGTG
GAGCGAATGGGGAAGCAGACAGGACACGCCCGTGGTGACTGGCGAAGCCGAGTGA

Upstream 100 bases:

>100_bases
TTGGCAAGATCGAAAAGAAGGGCAAGGAAAACCGCCGTTTCCGCATTCGCTTCGGCAGCTTGCCGCAAGCCTGAACCTGG
CCCATAGCTGGAGGAAAATC

Downstream 100 bases:

>100_bases
CATGCCTGAGAAATTGAAAGCGCGCATCACGCATCTGGAAATGACGGCCCGCTCGCCGCTCTCAGTACCGGTTCCTACGG
GCTTGCGGCTTGCTATCATA

Product: 3-mercaptopyruvate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTEKSAFVVSRDWLKERLHKPGLAIVDASWYLPAAGRNGQEEYEKAHIPGAVFFDQDKIADKESGLPHTLPSPEFFAQQV
GTLGITADETVVVYDGPGMFSAPRVWWMFRVMGVKNVYVLDGGFDGWKKAGYPVTDEVTKIAATFFKPSFNKDAVVDFQE
MRKIVDEKRSQIADARGAGRFTGRDAEPRAGMRSGHMPGARNVPVTTLSENGELKDLESLRRIFDEAGIDLSGPVVTSCG
SGVTAAVITLALTSLGHKDNRLYDGSWSEWGSRQDTPVVTGEAE

Sequences:

>Translated_284_residues
MTEKSAFVVSRDWLKERLHKPGLAIVDASWYLPAAGRNGQEEYEKAHIPGAVFFDQDKIADKESGLPHTLPSPEFFAQQV
GTLGITADETVVVYDGPGMFSAPRVWWMFRVMGVKNVYVLDGGFDGWKKAGYPVTDEVTKIAATFFKPSFNKDAVVDFQE
MRKIVDEKRSQIADARGAGRFTGRDAEPRAGMRSGHMPGARNVPVTTLSENGELKDLESLRRIFDEAGIDLSGPVVTSCG
SGVTAAVITLALTSLGHKDNRLYDGSWSEWGSRQDTPVVTGEAE
>Mature_283_residues
TEKSAFVVSRDWLKERLHKPGLAIVDASWYLPAAGRNGQEEYEKAHIPGAVFFDQDKIADKESGLPHTLPSPEFFAQQVG
TLGITADETVVVYDGPGMFSAPRVWWMFRVMGVKNVYVLDGGFDGWKKAGYPVTDEVTKIAATFFKPSFNKDAVVDFQEM
RKIVDEKRSQIADARGAGRFTGRDAEPRAGMRSGHMPGARNVPVTTLSENGELKDLESLRRIFDEAGIDLSGPVVTSCGS
GVTAAVITLALTSLGHKDNRLYDGSWSEWGSRQDTPVVTGEAE

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=273, Percent_Identity=41.7582417582418, Blast_Score=230, Evalue=9e-61,
Organism=Homo sapiens, GI61835204, Length=273, Percent_Identity=41.7582417582418, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI194473681, Length=273, Percent_Identity=41.7582417582418, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI17402865, Length=287, Percent_Identity=42.8571428571429, Blast_Score=219, Evalue=2e-57,
Organism=Escherichia coli, GI87082121, Length=276, Percent_Identity=44.5652173913043, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI87081967, Length=282, Percent_Identity=27.3049645390071, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71997283, Length=295, Percent_Identity=28.8135593220339, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17559150, Length=270, Percent_Identity=30, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17561888, Length=292, Percent_Identity=28.4246575342466, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI115534702, Length=237, Percent_Identity=28.2700421940928, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17543836, Length=239, Percent_Identity=28.4518828451883, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17543838, Length=239, Percent_Identity=28.4518828451883, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324825, Length=280, Percent_Identity=36.7857142857143, Blast_Score=172, Evalue=7e-44,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 31104; Mature: 30973

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKSAFVVSRDWLKERLHKPGLAIVDASWYLPAAGRNGQEEYEKAHIPGAVFFDQDKIA
CCCCCEEEEEHHHHHHHHCCCCEEEEECCEECCCCCCCCHHHHHHHCCCCEEEECCHHHC
DKESGLPHTLPSPEFFAQQVGTLGITADETVVVYDGPGMFSAPRVWWMFRVMGVKNVYVL
CHHCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEE
DGGFDGWKKAGYPVTDEVTKIAATFFKPSFNKDAVVDFQEMRKIVDEKRSQIADARGAGR
ECCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHCCCCC
FTGRDAEPRAGMRSGHMPGARNVPVTTLSENGELKDLESLRRIFDEAGIDLSGPVVTSCG
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHCCC
SGVTAAVITLALTSLGHKDNRLYDGSWSEWGSRQDTPVVTGEAE
CCHHHHHHHHHHHHCCCCCCEEECCCHHHCCCCCCCCEEECCCC
>Mature Secondary Structure 
TEKSAFVVSRDWLKERLHKPGLAIVDASWYLPAAGRNGQEEYEKAHIPGAVFFDQDKIA
CCCCEEEEEHHHHHHHHCCCCEEEEECCEECCCCCCCCHHHHHHHCCCCEEEECCHHHC
DKESGLPHTLPSPEFFAQQVGTLGITADETVVVYDGPGMFSAPRVWWMFRVMGVKNVYVL
CHHCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEE
DGGFDGWKKAGYPVTDEVTKIAATFFKPSFNKDAVVDFQEMRKIVDEKRSQIADARGAGR
ECCCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHCCCCC
FTGRDAEPRAGMRSGHMPGARNVPVTTLSENGELKDLESLRRIFDEAGIDLSGPVVTSCG
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHCCC
SGVTAAVITLALTSLGHKDNRLYDGSWSEWGSRQDTPVVTGEAE
CCHHHHHHHHHHHHCCCCCCEEECCCHHHCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]