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The map label for this gene is alaXM [H]

Identifier: 225852557

GI number: 225852557

Start: 1042449

End: 1043192

Strand: Direct

Name: alaXM [H]

Synonym: BMEA_A1095

Alternate gene names: 225852557

Gene position: 1042449-1043192 (Clockwise)

Preceding gene: 225852556

Following gene: 225852558

Centisome position: 49.04

GC content: 55.91

Gene sequence:

>744_bases
ATGGCATACGAGACTGAGGCGCTTTTTCGCGAAGATTCCTATCTTTCAACTGCGGAGGGCACAGTTCTCGCCATCGCCGG
AAATGGCGGCATTATCCTGGACAGGACGAATTTCTATGCTACTTCCGGCGGCCAGCCGGGGGATACCGGTTTCTTCGAGC
GCGCGGATGGTTCGCGGATCGAGATCGCGACGACCGTTACAGGCGAAACCAAGAATGAAATTGTCCATATGCCCGCGCAA
GGCCAGCAAATGCCGGACGTGGGCGAAAAACTCGTCCTGCATATAGATTGGCAGCGCCGCTACAAACTGATGCGTATGCA
CACGGCCTGCCATCTGCTTTCCGTCGCCTGCCCGTTCCCGATCACAGGTGCTGCGGTGGGCGAGGATGAAAGCCGTGTCG
ATTTCGACCTGCCGGATCAAAGCTATACGAAGGAATCCGTCACCGAAAAACTGATGGAACTGGTGCAGGCCAACGCCCCT
GTGTCGATCCACTGGATCAGCGACGAGGAATTTCTGGCCAATCCCGACATTGTGAAATCGAAAAATGTTCGCCCGCCCGT
TGGCCTTGGCCGTATCCGGCTGGTCGCCATTGGTGAAAATGGCTCTGTGGATTCGCAACCTTGCGGCGGCACACACGTTA
GTGAAACACAGGAAGTCGGTGAAATCCATATTGGCAAGATCGAAAAGAAGGGCAAGGAAAACCGCCGTTTCCGCATTCGC
TTCGGCAGCTTGCCGCAAGCCTGA

Upstream 100 bases:

>100_bases
CAAAGCTGTTCAATCCCGCCTTCCTCCGCGGCAAGAGCCTGCCCGTGCCGCGCTGGCTGGAGAAGAAAACCGAAATAGAC
ATACCGTTCGAGGGGTAATC

Downstream 100 bases:

>100_bases
ACCTGGCCCATAGCTGGAGGAAAATCATGACCGAGAAAAGCGCATTCGTCGTATCGCGCGACTGGTTGAAGGAACGTCTG
CATAAGCCCGGCCTCGCGAT

Product: alanyl-tRNA synthetase

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MAYETEALFREDSYLSTAEGTVLAIAGNGGIILDRTNFYATSGGQPGDTGFFERADGSRIEIATTVTGETKNEIVHMPAQ
GQQMPDVGEKLVLHIDWQRRYKLMRMHTACHLLSVACPFPITGAAVGEDESRVDFDLPDQSYTKESVTEKLMELVQANAP
VSIHWISDEEFLANPDIVKSKNVRPPVGLGRIRLVAIGENGSVDSQPCGGTHVSETQEVGEIHIGKIEKKGKENRRFRIR
FGSLPQA

Sequences:

>Translated_247_residues
MAYETEALFREDSYLSTAEGTVLAIAGNGGIILDRTNFYATSGGQPGDTGFFERADGSRIEIATTVTGETKNEIVHMPAQ
GQQMPDVGEKLVLHIDWQRRYKLMRMHTACHLLSVACPFPITGAAVGEDESRVDFDLPDQSYTKESVTEKLMELVQANAP
VSIHWISDEEFLANPDIVKSKNVRPPVGLGRIRLVAIGENGSVDSQPCGGTHVSETQEVGEIHIGKIEKKGKENRRFRIR
FGSLPQA
>Mature_246_residues
AYETEALFREDSYLSTAEGTVLAIAGNGGIILDRTNFYATSGGQPGDTGFFERADGSRIEIATTVTGETKNEIVHMPAQG
QQMPDVGEKLVLHIDWQRRYKLMRMHTACHLLSVACPFPITGAAVGEDESRVDFDLPDQSYTKESVTEKLMELVQANAPV
SIHWISDEEFLANPDIVKSKNVRPPVGLGRIRLVAIGENGSVDSQPCGGTHVSETQEVGEIHIGKIEKKGKENRRFRIRF
GSLPQA

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=213, Percent_Identity=29.1079812206573, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI217416402, Length=213, Percent_Identity=29.1079812206573, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1789048, Length=206, Percent_Identity=26.6990291262136, Blast_Score=61, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24582809, Length=234, Percent_Identity=29.9145299145299, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45552267, Length=234, Percent_Identity=29.9145299145299, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 27128; Mature: 26997

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYETEALFREDSYLSTAEGTVLAIAGNGGIILDRTNFYATSGGQPGDTGFFERADGSRI
CCCCCHHHHHCCCCEECCCCEEEEEECCCCEEEECCCEEEECCCCCCCCCCEECCCCCEE
EIATTVTGETKNEIVHMPAQGQQMPDVGEKLVLHIDWQRRYKLMRMHTACHLLSVACPFP
EEEEEECCCCCCCEEECCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCC
ITGAAVGEDESRVDFDLPDQSYTKESVTEKLMELVQANAPVSIHWISDEEFLANPDIVKS
CCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
KNVRPPVGLGRIRLVAIGENGSVDSQPCGGTHVSETQEVGEIHIGKIEKKGKENRRFRIR
CCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCEEEEE
FGSLPQA
ECCCCCC
>Mature Secondary Structure 
AYETEALFREDSYLSTAEGTVLAIAGNGGIILDRTNFYATSGGQPGDTGFFERADGSRI
CCCCHHHHHCCCCEECCCCEEEEEECCCCEEEECCCEEEECCCCCCCCCCEECCCCCEE
EIATTVTGETKNEIVHMPAQGQQMPDVGEKLVLHIDWQRRYKLMRMHTACHLLSVACPFP
EEEEEECCCCCCCEEECCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCC
ITGAAVGEDESRVDFDLPDQSYTKESVTEKLMELVQANAPVSIHWISDEEFLANPDIVKS
CCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
KNVRPPVGLGRIRLVAIGENGSVDSQPCGGTHVSETQEVGEIHIGKIEKKGKENRRFRIR
CCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCEEEEE
FGSLPQA
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]