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The map label for this gene is gatB [H]

Identifier: 225852412

GI number: 225852412

Start: 894616

End: 896118

Strand: Direct

Name: gatB [H]

Synonym: BMEA_A0939

Alternate gene names: 225852412

Gene position: 894616-896118 (Clockwise)

Preceding gene: 225852411

Following gene: 225852413

Centisome position: 42.09

GC content: 57.22

Gene sequence:

>1503_bases
ATGTCCATTATCGATACGCGTACACCTGAACCCAAACGCTTCATCTCTGGTGCGACCGGCGATTGGGAAGTCGTCATCGG
CATGGAAGTCCATGCGCAGGTCACGTCAGAATCGAAGCTGTTTTCCGGCGCTTCCACCGCATTCGGTGCCGAGCCGAACT
CCAATGTCTCTCTCGTGGATGCGGCCATGCCGGGCATGTTGCCGGTCATCAATCTGGAATGCGTGAGGCAGGCTGTGCGC
ACCGGCATCGGCTTGAATGCGCAGATCAATCTGAAGTCGGTCTTCGACCGCAAAAATTATTTCTATCCCGATTTGCCGCA
GGGCTACCAGATTTCGCAGTTCAAGCAGCCGATCGTCGGTGAGGGCAAGATCATGATTTCGGTCGGCCCGGACAACAAGG
GCCAGTTCGAGGACGTGGAGATCGGCATTGAGCGCCTGCATCTTGAGCAGGATGCGGGTAAATCCATGCACGACCAGCAC
CCGACCATGTCCTATGTGGACCTGAACCGTTCGGGCGTGGCGCTGATGGAGATCGTATCCAAGCCGGATCTGCGCTCGTC
GGATGAAGCGCGCGCTTACCTCACCAAGCTGCGCACGATTGTCCGCTATCTTGGCACCTGCGACGGCAATATGGATGAAG
GTTCCATGCGTGCTGACGTCAATGTTTCGGTGCGCCGTCCAGGCGGCGAATTCGGCACGCGTTGCGAGATCAAGAACGTC
AACTCGATCCGCTTTGTCGGCCAGGCCATCGAATATGAAGCGCGCCGCCAGATTGCCATTCTGGAAGATGGCGGCGTGAT
TGATCAGGAAACGCGCCTGTTCGATCCGGTGAAAGGCGAAACCCGTTCCATGCGCTCCAAGGAAGAGGCGCATGACTATC
GTTATTTCCCCGATCCGGACCTGTTGCCGCTTGAATTCGATCAGGCCTTCGTGGATGCGCTGGCGGCCAAATTGCCGGAA
CTGCCGGATGTGAAAAAGCAGCGCCTTGTCGAAACGCTTGGCATTTCCGTTTATGACGCCTCGATCCTTGTGACGGAGAA
GGCCATAGCCGATTATTATGAGGCGGTTGCCGAGGGGCGCGACGGCAAGGCTGCCGCAAACTGGGTCATCAACGATCTTC
TGGGCGCGCTGAACAAGGCGGGCAAGGATATCGAGGAATCGCCGATTAGCCCCGCCCAGCTTGGCGCCATCATCGATCTC
ATCAAGGAAGGTACGATTTCCGGTAAGATCGCAAAGGATCTTTTCGAGATCGTCTGGAATGAGGGTGGCGATCCGAAGAA
GCTCGTTGAAGAGCGCGGCATGAAGCAGGTTACAGATACCGGCGCGATTGAAAAGGCTGTCGATGACGTCATTGCCGCCA
ACCCGGACAAGGTTGAGCAGGCCAAGGCGAAGCCGACGCTGGCTGGCTGGTTTGTCGGTCAGGTCATGAAGGCGACGGGC
GGCAAGGCCAACCCGCAGGCCGTCAATGAACTGGTGAAATCCAAGCTGGGGATTGAGGAATAA

Upstream 100 bases:

>100_bases
TCGTTTGCGGACGGACGGCTGAAAAGCTTGCATCCACAAGGGCTTTGCTCTAGCCAGTAACCAACCAGTTTCAGCCAATC
ACAGTATTGACGAGCGAGTA

Downstream 100 bases:

>100_bases
TGTGGGTTCGCAGTGCCAGCGAGGCCGATCTTGAGGCTGTGCATGAATTGCTGGTCTCAACCTGGCACGCGACTTTCGAT
GATCTTTTGAGCCGGGAAAC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSIIDTRTPEPKRFISGATGDWEVVIGMEVHAQVTSESKLFSGASTAFGAEPNSNVSLVDAAMPGMLPVINLECVRQAVR
TGIGLNAQINLKSVFDRKNYFYPDLPQGYQISQFKQPIVGEGKIMISVGPDNKGQFEDVEIGIERLHLEQDAGKSMHDQH
PTMSYVDLNRSGVALMEIVSKPDLRSSDEARAYLTKLRTIVRYLGTCDGNMDEGSMRADVNVSVRRPGGEFGTRCEIKNV
NSIRFVGQAIEYEARRQIAILEDGGVIDQETRLFDPVKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQAFVDALAAKLPE
LPDVKKQRLVETLGISVYDASILVTEKAIADYYEAVAEGRDGKAAANWVINDLLGALNKAGKDIEESPISPAQLGAIIDL
IKEGTISGKIAKDLFEIVWNEGGDPKKLVEERGMKQVTDTGAIEKAVDDVIAANPDKVEQAKAKPTLAGWFVGQVMKATG
GKANPQAVNELVKSKLGIEE

Sequences:

>Translated_500_residues
MSIIDTRTPEPKRFISGATGDWEVVIGMEVHAQVTSESKLFSGASTAFGAEPNSNVSLVDAAMPGMLPVINLECVRQAVR
TGIGLNAQINLKSVFDRKNYFYPDLPQGYQISQFKQPIVGEGKIMISVGPDNKGQFEDVEIGIERLHLEQDAGKSMHDQH
PTMSYVDLNRSGVALMEIVSKPDLRSSDEARAYLTKLRTIVRYLGTCDGNMDEGSMRADVNVSVRRPGGEFGTRCEIKNV
NSIRFVGQAIEYEARRQIAILEDGGVIDQETRLFDPVKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQAFVDALAAKLPE
LPDVKKQRLVETLGISVYDASILVTEKAIADYYEAVAEGRDGKAAANWVINDLLGALNKAGKDIEESPISPAQLGAIIDL
IKEGTISGKIAKDLFEIVWNEGGDPKKLVEERGMKQVTDTGAIEKAVDDVIAANPDKVEQAKAKPTLAGWFVGQVMKATG
GKANPQAVNELVKSKLGIEE
>Mature_499_residues
SIIDTRTPEPKRFISGATGDWEVVIGMEVHAQVTSESKLFSGASTAFGAEPNSNVSLVDAAMPGMLPVINLECVRQAVRT
GIGLNAQINLKSVFDRKNYFYPDLPQGYQISQFKQPIVGEGKIMISVGPDNKGQFEDVEIGIERLHLEQDAGKSMHDQHP
TMSYVDLNRSGVALMEIVSKPDLRSSDEARAYLTKLRTIVRYLGTCDGNMDEGSMRADVNVSVRRPGGEFGTRCEIKNVN
SIRFVGQAIEYEARRQIAILEDGGVIDQETRLFDPVKGETRSMRSKEEAHDYRYFPDPDLLPLEFDQAFVDALAAKLPEL
PDVKKQRLVETLGISVYDASILVTEKAIADYYEAVAEGRDGKAAANWVINDLLGALNKAGKDIEESPISPAQLGAIIDLI
KEGTISGKIAKDLFEIVWNEGGDPKKLVEERGMKQVTDTGAIEKAVDDVIAANPDKVEQAKAKPTLAGWFVGQVMKATGG
KANPQAVNELVKSKLGIEE

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=41.2121212121212, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI115534094, Length=281, Percent_Identity=42.3487544483986, Blast_Score=214, Evalue=7e-56,
Organism=Saccharomyces cerevisiae, GI6319391, Length=524, Percent_Identity=31.2977099236641, Blast_Score=239, Evalue=7e-64,
Organism=Drosophila melanogaster, GI45550808, Length=488, Percent_Identity=39.1393442622951, Blast_Score=341, Evalue=9e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54715; Mature: 54584

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIDTRTPEPKRFISGATGDWEVVIGMEVHAQVTSESKLFSGASTAFGAEPNSNVSLVD
CCCCCCCCCCHHHHHCCCCCCEEEEEEEEEEEEECCCHHHHCCCCCCCCCCCCCCEEEEE
AAMPGMLPVINLECVRQAVRTGIGLNAQINLKSVFDRKNYFYPDLPQGYQISQFKQPIVG
HHCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCCCCCCHHHHCCCCCC
EGKIMISVGPDNKGQFEDVEIGIERLHLEQDAGKSMHDQHPTMSYVDLNRSGVALMEIVS
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHC
KPDLRSSDEARAYLTKLRTIVRYLGTCDGNMDEGSMRADVNVSVRRPGGEFGTRCEIKNV
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCC
NSIRFVGQAIEYEARRQIAILEDGGVIDQETRLFDPVKGETRSMRSKEEAHDYRYFPDPD
CHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCC
LLPLEFDQAFVDALAAKLPELPDVKKQRLVETLGISVYDASILVTEKAIADYYEAVAEGR
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEECCEEEEHHHHHHHHHHHHHCCC
DGKAAANWVINDLLGALNKAGKDIEESPISPAQLGAIIDLIKEGTISGKIAKDLFEIVWN
CCCHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
EGGDPKKLVEERGMKQVTDTGAIEKAVDDVIAANPDKVEQAKAKPTLAGWFVGQVMKATG
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCC
GKANPQAVNELVKSKLGIEE
CCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SIIDTRTPEPKRFISGATGDWEVVIGMEVHAQVTSESKLFSGASTAFGAEPNSNVSLVD
CCCCCCCCCHHHHHCCCCCCEEEEEEEEEEEEECCCHHHHCCCCCCCCCCCCCCEEEEE
AAMPGMLPVINLECVRQAVRTGIGLNAQINLKSVFDRKNYFYPDLPQGYQISQFKQPIVG
HHCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCCCCCCHHHHCCCCCC
EGKIMISVGPDNKGQFEDVEIGIERLHLEQDAGKSMHDQHPTMSYVDLNRSGVALMEIVS
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHC
KPDLRSSDEARAYLTKLRTIVRYLGTCDGNMDEGSMRADVNVSVRRPGGEFGTRCEIKNV
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCC
NSIRFVGQAIEYEARRQIAILEDGGVIDQETRLFDPVKGETRSMRSKEEAHDYRYFPDPD
CHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCC
LLPLEFDQAFVDALAAKLPELPDVKKQRLVETLGISVYDASILVTEKAIADYYEAVAEGR
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEECCEEEEHHHHHHHHHHHHHCCC
DGKAAANWVINDLLGALNKAGKDIEESPISPAQLGAIIDLIKEGTISGKIAKDLFEIVWN
CCCHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
EGGDPKKLVEERGMKQVTDTGAIEKAVDDVIAANPDKVEQAKAKPTLAGWFVGQVMKATG
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCC
GKANPQAVNELVKSKLGIEE
CCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA