Click here to switch to the map view.

The map label for this gene is irlS [H]

Identifier: 225852138

GI number: 225852138

Start: 622455

End: 623858

Strand: Direct

Name: irlS [H]

Synonym: BMEA_A0651

Alternate gene names: 225852138

Gene position: 622455-623858 (Clockwise)

Preceding gene: 225852137

Following gene: 225852139

Centisome position: 29.28

GC content: 59.05

Gene sequence:

>1404_bases
ATGAGCCGGTTTTCCGCACTCATGCGCACCACCGCGGCGCGGCTTTCCGCGCTTTATCTCCTGCTCTTTGCAGTGGGCGC
GGTTGCACTTGTCTTTTACATGACCAACCTTTCGGCTTCCATTCTGGCCGGGCAGACCCAACAGGCGCTGGGCGAGGAAG
TCGCGAGCATCGGCAAAAGCTATGCGCGCGGCGGTATTCCGCAACTTGTGCGAACCATTGATTATCGCTCGCGCCAGCCG
GGTGCCTATCTCTATCTTGTCGCCGACCCTACGGGCCGTATTCTTGCCGGTAATGTGGAAAGCGTTGAACCGGGCGTGCT
CAACACGGACGGTATTATCGAACGTGCCTTTACCTATCGCCGTTACGGCGAACAGGCGCCGCAGATGGAGCATCGCGCCA
TTGCCGTGGTGATTGCCCTGCCGAATGGAATGCGGCTGCTCGTCGGGCGGGATCTGGGCGAACCTGAGCGATTTCGCGAT
CTGATACGCAACTCGCTGGTGCTGGCGCTTGGCATCATGGGCGTGGGTGCGCTTTTGATCTGGCTCTTCGTGGGGCGGCG
CGCGCTCAAACGCATCGACGATGTGTCGCGCGCATCGCAACGCATCATGGATGGCGATCTGACCGGCAGGCTTCCCGTCA
ACGGCTCGGGCGACGAATTCGACCGGCTGTCTGGCAATCTCAATGTCATGCTGGCACGCATTCTGGAATTGAACGAGGGG
CTGAAACAGGTCTCCGACAATATTGCGCATGACCTGAAAACGCCGCTCACGCGGTTGCGAAACCGCGCTGAGGAGGCGCT
TGGCGGTGAGAAGGTAGAGCCGGAATATCGCGCTGCGCTGGAAGATATTATCGGCGAATCCGATCAGCTTATTCGCACGT
TCAATGCCATTTTGATGATCTCGCGGCTTGAAGCCGGTTATTCATCTGAAAACCTCGACGATATGCCGGTTGCCCCGATC
ATGCGCGATGTTGCGGAAATGTATGAGCCGGTGGCAGAGGATGCGGGTGTTACCTTGACGCTTGGTGCGCTTGATGATGT
AGCCCTGCACATCAACCGCGAACTGGTTGGCCAGACCGTGTCCAATCTGGTCGACAATGCGATCAAATATGCGGGCGGCG
AAGGACGCACGGCGACGGTCACGCTTTTGATGGAAAAGGATGCGCAATGGGTCCGTATCGTCGTGGCTGATAATGGCCCC
GGCATCCCCGCCGACAAACGTGACCATGCGACCGAGCGTTTCGTGCGCCTTGAAGAAAGCCGCACACAGCCGGGGTCTGG
CCTGGGTTTAAGTCTTGCCAAGTCGGTCATGAAGTTGCACGGTGGCGCGCTTCGTCTTGAGGACAACGGACCCGGCCTTC
GCGCCGTTCTGGAATTTCCGCTGCCGCATCGCGAGGTTGGCTAG

Upstream 100 bases:

>100_bases
AAAAGGGCTTTGACGAGCCGCTGCTTCATACGGTGCGCGGGGCTGGCTATATGCTGAAGGCGGGCCGGGGCAAGCAATCC
GCTGCCGCAAGGGCGGAATG

Downstream 100 bases:

>100_bases
AGCGCATCCCGAAAAGTGTGAAACTGTTTTCGCAAAAGATGCGCGTCAAAACAAAGGATTAGAGCGCCGACCTGATTCAA
TCAGATCGAAACGCGCTCTA

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSRFSALMRTTAARLSALYLLLFAVGAVALVFYMTNLSASILAGQTQQALGEEVASIGKSYARGGIPQLVRTIDYRSRQP
GAYLYLVADPTGRILAGNVESVEPGVLNTDGIIERAFTYRRYGEQAPQMEHRAIAVVIALPNGMRLLVGRDLGEPERFRD
LIRNSLVLALGIMGVGALLIWLFVGRRALKRIDDVSRASQRIMDGDLTGRLPVNGSGDEFDRLSGNLNVMLARILELNEG
LKQVSDNIAHDLKTPLTRLRNRAEEALGGEKVEPEYRAALEDIIGESDQLIRTFNAILMISRLEAGYSSENLDDMPVAPI
MRDVAEMYEPVAEDAGVTLTLGALDDVALHINRELVGQTVSNLVDNAIKYAGGEGRTATVTLLMEKDAQWVRIVVADNGP
GIPADKRDHATERFVRLEESRTQPGSGLGLSLAKSVMKLHGGALRLEDNGPGLRAVLEFPLPHREVG

Sequences:

>Translated_467_residues
MSRFSALMRTTAARLSALYLLLFAVGAVALVFYMTNLSASILAGQTQQALGEEVASIGKSYARGGIPQLVRTIDYRSRQP
GAYLYLVADPTGRILAGNVESVEPGVLNTDGIIERAFTYRRYGEQAPQMEHRAIAVVIALPNGMRLLVGRDLGEPERFRD
LIRNSLVLALGIMGVGALLIWLFVGRRALKRIDDVSRASQRIMDGDLTGRLPVNGSGDEFDRLSGNLNVMLARILELNEG
LKQVSDNIAHDLKTPLTRLRNRAEEALGGEKVEPEYRAALEDIIGESDQLIRTFNAILMISRLEAGYSSENLDDMPVAPI
MRDVAEMYEPVAEDAGVTLTLGALDDVALHINRELVGQTVSNLVDNAIKYAGGEGRTATVTLLMEKDAQWVRIVVADNGP
GIPADKRDHATERFVRLEESRTQPGSGLGLSLAKSVMKLHGGALRLEDNGPGLRAVLEFPLPHREVG
>Mature_466_residues
SRFSALMRTTAARLSALYLLLFAVGAVALVFYMTNLSASILAGQTQQALGEEVASIGKSYARGGIPQLVRTIDYRSRQPG
AYLYLVADPTGRILAGNVESVEPGVLNTDGIIERAFTYRRYGEQAPQMEHRAIAVVIALPNGMRLLVGRDLGEPERFRDL
IRNSLVLALGIMGVGALLIWLFVGRRALKRIDDVSRASQRIMDGDLTGRLPVNGSGDEFDRLSGNLNVMLARILELNEGL
KQVSDNIAHDLKTPLTRLRNRAEEALGGEKVEPEYRAALEDIIGESDQLIRTFNAILMISRLEAGYSSENLDDMPVAPIM
RDVAEMYEPVAEDAGVTLTLGALDDVALHINRELVGQTVSNLVDNAIKYAGGEGRTATVTLLMEKDAQWVRIVVADNGPG
IPADKRDHATERFVRLEESRTQPGSGLGLSLAKSVMKLHGGALRLEDNGPGLRAVLEFPLPHREVG

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=300, Percent_Identity=25.3333333333333, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787894, Length=308, Percent_Identity=29.2207792207792, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788393, Length=279, Percent_Identity=26.8817204301075, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788279, Length=280, Percent_Identity=24.2857142857143, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789808, Length=317, Percent_Identity=29.0220820189274, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789403, Length=251, Percent_Identity=28.2868525896414, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790861, Length=340, Percent_Identity=27.3529411764706, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786912, Length=200, Percent_Identity=28, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790346, Length=283, Percent_Identity=26.8551236749117, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786600, Length=222, Percent_Identity=28.3783783783784, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787374, Length=230, Percent_Identity=26.5217391304348, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790551, Length=287, Percent_Identity=22.9965156794425, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788713, Length=239, Percent_Identity=22.1757322175732, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1789149, Length=211, Percent_Identity=27.4881516587678, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50873; Mature: 50742

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFSALMRTTAARLSALYLLLFAVGAVALVFYMTNLSASILAGQTQQALGEEVASIGKS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHH
YARGGIPQLVRTIDYRSRQPGAYLYLVADPTGRILAGNVESVEPGVLNTDGIIERAFTYR
HHCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCHHHHHHHHHH
RYGEQAPQMEHRAIAVVIALPNGMRLLVGRDLGEPERFRDLIRNSLVLALGIMGVGALLI
HHCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WLFVGRRALKRIDDVSRASQRIMDGDLTGRLPVNGSGDEFDRLSGNLNVMLARILELNEG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
LKQVSDNIAHDLKTPLTRLRNRAEEALGGEKVEPEYRAALEDIIGESDQLIRTFNAILMI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
SRLEAGYSSENLDDMPVAPIMRDVAEMYEPVAEDAGVTLTLGALDDVALHINRELVGQTV
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHH
SNLVDNAIKYAGGEGRTATVTLLMEKDAQWVRIVVADNGPGIPADKRDHATERFVRLEES
HHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
RTQPGSGLGLSLAKSVMKLHGGALRLEDNGPGLRAVLEFPLPHREVG
CCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCEEHEEECCCCCCCCC
>Mature Secondary Structure 
SRFSALMRTTAARLSALYLLLFAVGAVALVFYMTNLSASILAGQTQQALGEEVASIGKS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHH
YARGGIPQLVRTIDYRSRQPGAYLYLVADPTGRILAGNVESVEPGVLNTDGIIERAFTYR
HHCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCHHHHHHHHHH
RYGEQAPQMEHRAIAVVIALPNGMRLLVGRDLGEPERFRDLIRNSLVLALGIMGVGALLI
HHCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WLFVGRRALKRIDDVSRASQRIMDGDLTGRLPVNGSGDEFDRLSGNLNVMLARILELNEG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
LKQVSDNIAHDLKTPLTRLRNRAEEALGGEKVEPEYRAALEDIIGESDQLIRTFNAILMI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
SRLEAGYSSENLDDMPVAPIMRDVAEMYEPVAEDAGVTLTLGALDDVALHINRELVGQTV
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHH
SNLVDNAIKYAGGEGRTATVTLLMEKDAQWVRIVVADNGPGIPADKRDHATERFVRLEES
HHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
RTQPGSGLGLSLAKSVMKLHGGALRLEDNGPGLRAVLEFPLPHREVG
CCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCEEHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]