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The map label for this gene is ygcA [C]

Identifier: 225851974

GI number: 225851974

Start: 458030

End: 459277

Strand: Direct

Name: ygcA [C]

Synonym: BMEA_A0471

Alternate gene names: 225851974

Gene position: 458030-459277 (Clockwise)

Preceding gene: 225851973

Following gene: 225851976

Centisome position: 21.55

GC content: 63.54

Gene sequence:

>1248_bases
ATGACGTCGTCGAAGACGGGGCAAATAACGATCCGCTCGATTGGTGCGGGCGGCGACGGCGTCGCAAACCTTCCCGATGG
GCAGATCTATGTGCCTTTCACGCTTCCCGGTGAAGTGGTGAATGTGGCGCGCGACAAGAACCGCGCCACGCTGATGGCGC
TGCTGGAAGCCTCGCCCGAACGGCAGAACCCGGCCTGCCGTCATTTCGAGGATTGTGGCGGTTGTGCTCTGCAACACTGG
CAGGATGAACCTTATCGCCTGTGGAAGCGTGAGCTTGTTGTCGGGGCGCTGAAGGGCAGGGGCACCGATGTGGAGGTTGC
GCCGCTGGTGGCGTGCAACCCGCATACGCGCCGCCGCGCCGTCTTCGCCGCGCGCAAGACGGAAAAGGGCGTGCTGCTTG
GCTTCAACCGCCACCAGAGCCACGAGATCATCGATATTGTCGAATGCCCGGTCACGGTGCCGGAAATCATCGCGCGTCTG
GATGACCTGCGCGAAGTGGGCGCCTTGCTTGCGCCCGGCTCCGGGCCGTTCAAGCTGGCGGCAACGCTGACGGAATCCGG
GCTCGATCTTGCGGCAAGCGGCTGCGGCAAGCTCAACGACGAGCAGCGCCGCGCCTTGACGGCGTTGGTTATCAAAAAGG
ATTTTGCGCGCCTTTCGCATGAGGGCGAAATCATTGTCGAGCCGAAAAAGCCACTCATCCATTTCGGCAAGGTGCCTGTG
CCGGTTCCGCCCGGCTGCTTCCTGCAGGCGACTGCGGAGGCGGAGGAAACGATGGCTGCGCTGGTGCTGGCCCATCTGGG
CAAGGCCCGGCGCGTGGCCGATCTTTTCTGCGGTGTCGGTACTTTTGCGCTCCGCATCGCGGAAAAGAGCGCGGTCCATG
CGGTGGAAAACGATGCAGCGGCGCTGGCCGCACTCGATCGCGGCGTGCGCCATGTGCAGGGGCTTAAACCCGTTTCCATC
GAGCGGCGCGACCTGTTCCGCCGCCCGCTGATGACGAAGGAGCTTCTGCCCTATAACGCCGTGGTCTTCGATCCGCCCCG
CGCTGGTGCGGAAGAACAGGCGCTGGAACTGGCCAAGTCCAAGGTGGAAAAGGTGGTTGCCATTTCCTGCAATCCGGTGA
CGCTGGCCCGCGATCTGGCCATTCTCCAGAAGGGCGGCTACCGCATCGAGCGCGTCACGCCCATCGACCAATTCCTCTGG
TCTGCGCATGTGGAGGCGGTGGCGGTGCTGACGAAGGGAAGGCAATAA

Upstream 100 bases:

>100_bases
TGGAAACGCAGCCCGGCTGGCGTGCTCTCGGCCTTTGTCCGTCGCCCATCGAAGGCGGCGACGGCAATCGTGAATATCTT
CTGGCAGGAAAGAAGGACAG

Downstream 100 bases:

>100_bases
GGCAGTAGGGCAGTATGTTAAGGGAATAGGGGAATAAGGGAGTAGGGGAGTATGTTTTGGTTGCGCATGAGCGCAGCGAC
CATATTCTTTACTGCCCTAC

Product: tRNA (Uracil-5-)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTSSKTGQITIRSIGAGGDGVANLPDGQIYVPFTLPGEVVNVARDKNRATLMALLEASPERQNPACRHFEDCGGCALQHW
QDEPYRLWKRELVVGALKGRGTDVEVAPLVACNPHTRRRAVFAARKTEKGVLLGFNRHQSHEIIDIVECPVTVPEIIARL
DDLREVGALLAPGSGPFKLAATLTESGLDLAASGCGKLNDEQRRALTALVIKKDFARLSHEGEIIVEPKKPLIHFGKVPV
PVPPGCFLQATAEAEETMAALVLAHLGKARRVADLFCGVGTFALRIAEKSAVHAVENDAAALAALDRGVRHVQGLKPVSI
ERRDLFRRPLMTKELLPYNAVVFDPPRAGAEEQALELAKSKVEKVVAISCNPVTLARDLAILQKGGYRIERVTPIDQFLW
SAHVEAVAVLTKGRQ

Sequences:

>Translated_415_residues
MTSSKTGQITIRSIGAGGDGVANLPDGQIYVPFTLPGEVVNVARDKNRATLMALLEASPERQNPACRHFEDCGGCALQHW
QDEPYRLWKRELVVGALKGRGTDVEVAPLVACNPHTRRRAVFAARKTEKGVLLGFNRHQSHEIIDIVECPVTVPEIIARL
DDLREVGALLAPGSGPFKLAATLTESGLDLAASGCGKLNDEQRRALTALVIKKDFARLSHEGEIIVEPKKPLIHFGKVPV
PVPPGCFLQATAEAEETMAALVLAHLGKARRVADLFCGVGTFALRIAEKSAVHAVENDAAALAALDRGVRHVQGLKPVSI
ERRDLFRRPLMTKELLPYNAVVFDPPRAGAEEQALELAKSKVEKVVAISCNPVTLARDLAILQKGGYRIERVTPIDQFLW
SAHVEAVAVLTKGRQ
>Mature_414_residues
TSSKTGQITIRSIGAGGDGVANLPDGQIYVPFTLPGEVVNVARDKNRATLMALLEASPERQNPACRHFEDCGGCALQHWQ
DEPYRLWKRELVVGALKGRGTDVEVAPLVACNPHTRRRAVFAARKTEKGVLLGFNRHQSHEIIDIVECPVTVPEIIARLD
DLREVGALLAPGSGPFKLAATLTESGLDLAASGCGKLNDEQRRALTALVIKKDFARLSHEGEIIVEPKKPLIHFGKVPVP
VPPGCFLQATAEAEETMAALVLAHLGKARRVADLFCGVGTFALRIAEKSAVHAVENDAAALAALDRGVRHVQGLKPVSIE
RRDLFRRPLMTKELLPYNAVVFDPPRAGAEEQALELAKSKVEKVVAISCNPVTLARDLAILQKGGYRIERVTPIDQFLWS
AHVEAVAVLTKGRQ

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789148, Length=439, Percent_Identity=28.4738041002278, Blast_Score=135, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 44988; Mature: 44856

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSKTGQITIRSIGAGGDGVANLPDGQIYVPFTLPGEVVNVARDKNRATLMALLEASPE
CCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCHHHHHHHHHCCCC
RQNPACRHFEDCGGCALQHWQDEPYRLWKRELVVGALKGRGTDVEVAPLVACNPHTRRRA
CCCCCHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHH
VFAARKTEKGVLLGFNRHQSHEIIDIVECPVTVPEIIARLDDLREVGALLAPGSGPFKLA
HHHHHCCCCCEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCEEEE
ATLTESGLDLAASGCGKLNDEQRRALTALVIKKDFARLSHEGEIIVEPKKPLIHFGKVPV
EEEHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEECCCCC
PVPPGCFLQATAEAEETMAALVLAHLGKARRVADLFCGVGTFALRIAEKSAVHAVENDAA
CCCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ALAALDRGVRHVQGLKPVSIERRDLFRRPLMTKELLPYNAVVFDPPRAGAEEQALELAKS
HHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHH
KVEKVVAISCNPVTLARDLAILQKGGYRIERVTPIDQFLWSAHVEAVAVLTKGRQ
HHHEEEEEECCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHEEECCCCC
>Mature Secondary Structure 
TSSKTGQITIRSIGAGGDGVANLPDGQIYVPFTLPGEVVNVARDKNRATLMALLEASPE
CCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCHHHHHHHHHCCCC
RQNPACRHFEDCGGCALQHWQDEPYRLWKRELVVGALKGRGTDVEVAPLVACNPHTRRRA
CCCCCHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHH
VFAARKTEKGVLLGFNRHQSHEIIDIVECPVTVPEIIARLDDLREVGALLAPGSGPFKLA
HHHHHCCCCCEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCEEEE
ATLTESGLDLAASGCGKLNDEQRRALTALVIKKDFARLSHEGEIIVEPKKPLIHFGKVPV
EEEHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEECCCCC
PVPPGCFLQATAEAEETMAALVLAHLGKARRVADLFCGVGTFALRIAEKSAVHAVENDAA
CCCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ALAALDRGVRHVQGLKPVSIERRDLFRRPLMTKELLPYNAVVFDPPRAGAEEQALELAKS
HHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHH
KVEKVVAISCNPVTLARDLAILQKGGYRIERVTPIDQFLWSAHVEAVAVLTKGRQ
HHHEEEEEECCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]