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The map label for this gene is ispB [H]

Identifier: 225851937

GI number: 225851937

Start: 424650

End: 425672

Strand: Direct

Name: ispB [H]

Synonym: BMEA_A0432

Alternate gene names: 225851937

Gene position: 424650-425672 (Clockwise)

Preceding gene: 225851928

Following gene: 225851938

Centisome position: 19.98

GC content: 61.88

Gene sequence:

>1023_bases
ATGGCATTGGGTGTGGTTCTGAATCTCGACGGTAAAATGAAGCAGGAAGGATCGGTGCAGCCGCTGGTCGACCTCACCAG
GGCCGATATGGCGCGCGTGAACGAGTTGATCCTGTCCCGCGCGGGGTCCGATGTCGAAATGATACCGGAGGTTGCCAACC
ACCTCATTTCATCCGGCGGCAAGCGCCTGCGCCCGATGATGACCCTGGCCTCCGCCCGCATGTTCGGCTATGAGGGCGAT
GGCCATGTGCGCCTTGCAACCTCGGTCGAATTCATGCACACGGCGACCCTCCTGCACGATGATGTGGTCGATGAAAGCGA
CCTGCGCCGCGGCAAGAGCACGGCGCGCACGATCTGGGGCAACCAGGCCAGTGTTCTCGTCGGCGATTTCCTGCTGGGTC
AGGCGTTCAAGATGATGGTCGATGTCGGCTCGCTCGATGCGCTCGACGTGCTGGCCACCTCCGCCTCCATCATTGCGGAA
GGCGAGGTGATGCAGCTTGCCGCAGCCAAGAACATGGAAACCACCGAAGACGAATATCTGGCAGTCATCAAGGCGAAGAC
TGCCGCGCTCTTTTCAGCGGCCGCGGAAGTGGGGCCGATCATTGCCGGCGCGCAACGCTCCGACCGCGCGGCCCTGCGCG
ATTACGGCCTCAATCTGGGCCTTGCTTTCCAGCTTGTTGACGATGCGCTTGATTATGGCGGTTCGGCTGCCGATCTCGGC
AAGAATACCGGCGACGATTTCCGCGAAGGCAAGATCACGCTTCCCGTCATCCTCAGCTATCGCCGTGGCACCGACGAGGA
GCGCGCTTTCTGGAAAGGGGCTATCGAAGGCGGCGCGAGCGACGACGCTTCCTTGCAGAAGGCAATCGGCCTGATGATGA
AACACGGCGCGATTGCCGACACGGTGCAGCGCGCCCGCCATTTCGGCGAGATCGCACGCGATGCGCTGGCCCCGCTCAAG
GCGTCGCCGCAAAAGGATGCGCTGATCGAGGTGGTCGATTTCTGCATCAGCCGTGTCAATTGA

Upstream 100 bases:

>100_bases
TTTTGCGGCCAGGGGAATCACCCGGCGCGGCATGATGTGGTCGAAACGAAAAGTTGGAGCCTTAGCCCGACCTGACCGGG
CGCACGCCGCTCCAGGAGAA

Downstream 100 bases:

>100_bases
TCGTCAATTGATCCGGGCCGGGCGCGGTGGCGGACCTTTCGCCGCTGCAATTTGAAGGCTTCGTGGCTGACCGGGGCGCC
TATGAGGGATGCTTTTCTTG

Product: hypothetical protein

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MALGVVLNLDGKMKQEGSVQPLVDLTRADMARVNELILSRAGSDVEMIPEVANHLISSGGKRLRPMMTLASARMFGYEGD
GHVRLATSVEFMHTATLLHDDVVDESDLRRGKSTARTIWGNQASVLVGDFLLGQAFKMMVDVGSLDALDVLATSASIIAE
GEVMQLAAAKNMETTEDEYLAVIKAKTAALFSAAAEVGPIIAGAQRSDRAALRDYGLNLGLAFQLVDDALDYGGSAADLG
KNTGDDFREGKITLPVILSYRRGTDEERAFWKGAIEGGASDDASLQKAIGLMMKHGAIADTVQRARHFGEIARDALAPLK
ASPQKDALIEVVDFCISRVN

Sequences:

>Translated_340_residues
MALGVVLNLDGKMKQEGSVQPLVDLTRADMARVNELILSRAGSDVEMIPEVANHLISSGGKRLRPMMTLASARMFGYEGD
GHVRLATSVEFMHTATLLHDDVVDESDLRRGKSTARTIWGNQASVLVGDFLLGQAFKMMVDVGSLDALDVLATSASIIAE
GEVMQLAAAKNMETTEDEYLAVIKAKTAALFSAAAEVGPIIAGAQRSDRAALRDYGLNLGLAFQLVDDALDYGGSAADLG
KNTGDDFREGKITLPVILSYRRGTDEERAFWKGAIEGGASDDASLQKAIGLMMKHGAIADTVQRARHFGEIARDALAPLK
ASPQKDALIEVVDFCISRVN
>Mature_339_residues
ALGVVLNLDGKMKQEGSVQPLVDLTRADMARVNELILSRAGSDVEMIPEVANHLISSGGKRLRPMMTLASARMFGYEGDG
HVRLATSVEFMHTATLLHDDVVDESDLRRGKSTARTIWGNQASVLVGDFLLGQAFKMMVDVGSLDALDVLATSASIIAEG
EVMQLAAAKNMETTEDEYLAVIKAKTAALFSAAAEVGPIIAGAQRSDRAALRDYGLNLGLAFQLVDDALDYGGSAADLGK
NTGDDFREGKITLPVILSYRRGTDEERAFWKGAIEGGASDDASLQKAIGLMMKHGAIADTVQRARHFGEIARDALAPLKA
SPQKDALIEVVDFCISRVN

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=296, Percent_Identity=27.3648648648649, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4758430, Length=209, Percent_Identity=24.8803827751196, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI83700220, Length=209, Percent_Identity=24.8803827751196, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=45.7943925233645, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1786623, Length=223, Percent_Identity=34.9775784753363, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17505681, Length=237, Percent_Identity=32.9113924050633, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319475, Length=292, Percent_Identity=31.1643835616438, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6325188, Length=241, Percent_Identity=22.8215767634855, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24651612, Length=312, Percent_Identity=30.7692307692308, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI281365769, Length=295, Percent_Identity=24.0677966101695, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24660002, Length=295, Percent_Identity=24.0677966101695, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36352; Mature: 36221

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALGVVLNLDGKMKQEGSVQPLVDLTRADMARVNELILSRAGSDVEMIPEVANHLISSGG
CCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
KRLRPMMTLASARMFGYEGDGHVRLATSVEFMHTATLLHDDVVDESDLRRGKSTARTIWG
HHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHCC
NQASVLVGDFLLGQAFKMMVDVGSLDALDVLATSASIIAEGEVMQLAAAKNMETTEDEYL
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHE
AVIKAKTAALFSAAAEVGPIIAGAQRSDRAALRDYGLNLGLAFQLVDDALDYGGSAADLG
EHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHCC
KNTGDDFREGKITLPVILSYRRGTDEERAFWKGAIEGGASDDASLQKAIGLMMKHGAIAD
CCCCCCHHCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHH
TVQRARHFGEIARDALAPLKASPQKDALIEVVDFCISRVN
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALGVVLNLDGKMKQEGSVQPLVDLTRADMARVNELILSRAGSDVEMIPEVANHLISSGG
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
KRLRPMMTLASARMFGYEGDGHVRLATSVEFMHTATLLHDDVVDESDLRRGKSTARTIWG
HHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHCC
NQASVLVGDFLLGQAFKMMVDVGSLDALDVLATSASIIAEGEVMQLAAAKNMETTEDEYL
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHE
AVIKAKTAALFSAAAEVGPIIAGAQRSDRAALRDYGLNLGLAFQLVDDALDYGGSAADLG
EHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHCC
KNTGDDFREGKITLPVILSYRRGTDEERAFWKGAIEGGASDDASLQKAIGLMMKHGAIAD
CCCCCCHHCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHH
TVQRARHFGEIARDALAPLKASPQKDALIEVVDFCISRVN
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]