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The map label for this gene is norM

Identifier: 225851882

GI number: 225851882

Start: 368365

End: 369780

Strand: Direct

Name: norM

Synonym: BMEA_A0375

Alternate gene names: 225851882

Gene position: 368365-369780 (Clockwise)

Preceding gene: 225851879

Following gene: 225851884

Centisome position: 17.33

GC content: 62.57

Gene sequence:

>1416_bases
ATGGACGGAACATTTGACGCGGGCTTTCGCGAGCCAACGATATCGAAGGCGAACCGCTGGGGCAGGGAGATGGTGGTTGC
GCTGAAGCTCGGCTGGCCGCTGATCTTCACCAATCTCTCGCAGGCGGCCCTCACCGCCACCGATGTGATCTTCATCGGGC
GTCTGGGGGCGGACACACTGGCTTCCGCCCTGCTTGCGACGAGTTTCTATCACACGCTGATGATCTTTTCGATGGGCCTT
GTCTCGGCCGTCATGCCGATGATCGCCATCGCGCTCGGCAAGAACCGCCATTCCGTGCGCGATGTGCGCCGCACGGTGCG
GCAGGGCTTCTGGTCGGCGATCATGATCGTGATCCCTCTCTGGGTGGTGCTGTGGCACTGTGAGGAGATTTTCCTCTTCC
TCGGCCAGCGCCCGGATATTGCCGCGCGCTCGACCGACTTCATGCACACGCTGCAATGGGCGCTGCTGCCCTATCTCTTC
TATATCGTCCTGCGGTCTTTCTTCGCGGCAATGGAAAAGCCCATGTGGACGCTGCTCGTGGCGGCGCTTGCCATTGGCTT
CAATGCACTTGCGGGCTGGACGCTGATCTTCGGCCATTTCGGCTTTGCGCCGATGGGGCTGCATGGTGCGGGCATGGCGA
CAACGGCATCCAGCACGATGATGTTCCTCGGCCTTGCCTTCATCACGCTGCGTCATCCCCGCTTCCGCCGTTATCACCTG
TTCGGTCGCTTCTGGCGCCCCGACTGGCCGCGCCTGATCGAGCTTTGGCGCATCGGCCTGCCCATGGCGCTGACCTTCGT
GTTTGAAACCTCGATCTTCTATGCCGCCGTGGTGATGATGGGCCGCATCGGGCCGACCGCCATGGCCGCCCATGCGGTGG
CGATCCAGATTGCATCGCTCAGTTTCATGGTTCCACTCGGCTTCGGGCAGGTGGCCACGGTGCGTGTAGGGCGGGCCTAT
GGCAGGGGTGATCCGAAAGCCATTGCTTATGCGGGCTGGAGCGCCTATGCGCTCGGCGTCGGCTTCATGGCGTTGATGGG
CATCCTGATGGTTCTCATGCCACGGGTCTTCATCGGCATCTTCCTGAACCTCAACGATCCGCAAAATCTGCCGGTCATGG
AACTTGCCGTGACATTCCTTGCGCTTGCAGCCCTGTTCCAGATCGTGGACGGCGCACAGGCTGTGGCGGCGGGCATGTTG
CGCGGCTTGCGCGATACGCGCATTCCGATGTTGCTTGCCCTTTTCGGCTATTGGGGTGTCGGCTTGCCGCTCGGCGCGGT
TCTGGCCTTCCAGTTCGGAATGGGGGGCGTCGGCATCTGGCTTGGCCTTGCGGCGGGGCTTGGCATGGTGGCCGTGCTGA
TGACGATACGCTGGCGCCGGCATCTGGCACATGTCTCGGCCGTTGCCGCGGCGTAA

Upstream 100 bases:

>100_bases
CTCTTCCCAGATTGACATGGTTTCTGATGGTTCGTGGCGTGGCCTCATTACATGGAGCATCCGGCAGATTGACGTCTCCG
GATGCTGCGGGGAGCGAAAT

Downstream 100 bases:

>100_bases
AAAGAGCGCCGATCTGGTTTAATCAGACCGGCGCTCGGGTAGTCTTTACTTGGTCAATGCCTGAATGCGGTCGAGCGCGG
CACCGAAGCCTTTCAGGGAA

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MDGTFDAGFREPTISKANRWGREMVVALKLGWPLIFTNLSQAALTATDVIFIGRLGADTLASALLATSFYHTLMIFSMGL
VSAVMPMIAIALGKNRHSVRDVRRTVRQGFWSAIMIVIPLWVVLWHCEEIFLFLGQRPDIAARSTDFMHTLQWALLPYLF
YIVLRSFFAAMEKPMWTLLVAALAIGFNALAGWTLIFGHFGFAPMGLHGAGMATTASSTMMFLGLAFITLRHPRFRRYHL
FGRFWRPDWPRLIELWRIGLPMALTFVFETSIFYAAVVMMGRIGPTAMAAHAVAIQIASLSFMVPLGFGQVATVRVGRAY
GRGDPKAIAYAGWSAYALGVGFMALMGILMVLMPRVFIGIFLNLNDPQNLPVMELAVTFLALAALFQIVDGAQAVAAGML
RGLRDTRIPMLLALFGYWGVGLPLGAVLAFQFGMGGVGIWLGLAAGLGMVAVLMTIRWRRHLAHVSAVAAA

Sequences:

>Translated_471_residues
MDGTFDAGFREPTISKANRWGREMVVALKLGWPLIFTNLSQAALTATDVIFIGRLGADTLASALLATSFYHTLMIFSMGL
VSAVMPMIAIALGKNRHSVRDVRRTVRQGFWSAIMIVIPLWVVLWHCEEIFLFLGQRPDIAARSTDFMHTLQWALLPYLF
YIVLRSFFAAMEKPMWTLLVAALAIGFNALAGWTLIFGHFGFAPMGLHGAGMATTASSTMMFLGLAFITLRHPRFRRYHL
FGRFWRPDWPRLIELWRIGLPMALTFVFETSIFYAAVVMMGRIGPTAMAAHAVAIQIASLSFMVPLGFGQVATVRVGRAY
GRGDPKAIAYAGWSAYALGVGFMALMGILMVLMPRVFIGIFLNLNDPQNLPVMELAVTFLALAALFQIVDGAQAVAAGML
RGLRDTRIPMLLALFGYWGVGLPLGAVLAFQFGMGGVGIWLGLAAGLGMVAVLMTIRWRRHLAHVSAVAAA
>Mature_471_residues
MDGTFDAGFREPTISKANRWGREMVVALKLGWPLIFTNLSQAALTATDVIFIGRLGADTLASALLATSFYHTLMIFSMGL
VSAVMPMIAIALGKNRHSVRDVRRTVRQGFWSAIMIVIPLWVVLWHCEEIFLFLGQRPDIAARSTDFMHTLQWALLPYLF
YIVLRSFFAAMEKPMWTLLVAALAIGFNALAGWTLIFGHFGFAPMGLHGAGMATTASSTMMFLGLAFITLRHPRFRRYHL
FGRFWRPDWPRLIELWRIGLPMALTFVFETSIFYAAVVMMGRIGPTAMAAHAVAIQIASLSFMVPLGFGQVATVRVGRAY
GRGDPKAIAYAGWSAYALGVGFMALMGILMVLMPRVFIGIFLNLNDPQNLPVMELAVTFLALAALFQIVDGAQAVAAGML
RGLRDTRIPMLLALFGYWGVGLPLGAVLAFQFGMGGVGIWLGLAAGLGMVAVLMTIRWRRHLAHVSAVAAA

Specific function: Multidrug efflux pump

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Homologues:

Organism=Homo sapiens, GI153792564, Length=447, Percent_Identity=24.3847874720358, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI38261960, Length=482, Percent_Identity=22.8215767634855, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI22907060, Length=426, Percent_Identity=23.4741784037559, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI48994890, Length=447, Percent_Identity=29.3064876957494, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI226510954, Length=439, Percent_Identity=24.8291571753986, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320545, Length=437, Percent_Identity=24.0274599542334, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NORM_BRUME (Q8YFD7)

Other databases:

- EMBL:   AE008917
- PIR:   AC3450
- RefSeq:   NP_540502.1
- GeneID:   1197296
- GenomeReviews:   AE008917_GR
- KEGG:   bme:BMEI1585
- HOGENOM:   HBG479615
- OMA:   AFWAGWR
- PhylomeDB:   Q8YFD7
- ProtClustDB:   CLSK864142
- BioCyc:   BMEL224914:BMEI1585-MONOMER
- InterPro:   IPR002528
- PANTHER:   PTHR11206
- TIGRFAMs:   TIGR00797

Pfam domain/function: PF01554 MatE

EC number: NA

Molecular weight: Translated: 51686; Mature: 51686

Theoretical pI: Translated: 11.29; Mature: 11.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x20c48404)-; HASH(0x209cc60c)-; HASH(0x1f2f6960)-; HASH(0x1f2f696c)-; HASH(0x1fdc30b0)-; HASH(0x20a6fe10)-; HASH(0x20ee7bf8)-; HASH(0x207832b8)-; HASH(0x20fd1730)-; HASH(0x209fc698)-; HASH(0x205862dc)-; HASH(0x20dfb60c)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure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HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11756688