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The map label for this gene is kdsB

Identifier: 225851581

GI number: 225851581

Start: 43154

End: 43909

Strand: Reverse

Name: kdsB

Synonym: BMEA_A0040

Alternate gene names: 225851581

Gene position: 43909-43154 (Counterclockwise)

Preceding gene: 225851583

Following gene: 225851580

Centisome position: 2.07

GC content: 60.45

Gene sequence:

>756_bases
ATGCTTCAGACAATGAAAACCCTGACGCTCATTCCGGCCCGGCTGGGCTCCACCCGCCTGCCGAACAAGCCGCTTGCCGA
TATTTGCGGCAAGCCCATGATCGTGCATGTGGCGGATCGGGCAGCCGCGGCAAAGCTTGGCCGCACGGTGATCGCAACCG
ACAGTGAAGAGATTTTCAAGGTCGTGGCCGCCCACGGCCATGAAGCGATCATGACGCGCGGAGACCATGAATCCGGTTCC
GATCGCATCTATGAAGCACTGGCGAAGCTCGATCCATCGGGCGAAATCGATGCCGTGGTCAATGTGCAGGGCGATCTGCC
AACCATCGATCCCGACACGATCCGCCGCGCGCTCCTGCCGCTGGAGGACGGGCCTGCGGATATCGCAACGCTTGGCGTAG
AGATCACGGTCGAAGAAGAAAAGACCAATCCGAATGTGGTCAAGATCGTCGGCTCCCCGCTGGCGGGCAATAGGCGCCTG
CGCGCGCTTTATTTCACCCGCGCCACCGCGCCTTATGGCGAAGGCCCGCTCTATCATCATATCGGCCTTTACGCCTATCG
CCGTTCTGCCCTTGAACGCTTCGTGAAACTTGGGCCATCGCCGCTGGAAAAGCGCGAGAAGCTGGAGCAGTTGCGCGCAC
TTGAAGCTGGAATGCGCATCGATGTCGAAATCGTGAAGACCGTGCCGCTTGGCGTCGATACGCAGGCCGATCTTGACCGC
GCCCGGACATTTTGCAGCCAGGCGGGAACCATTTGA

Upstream 100 bases:

>100_bases
TGTCGGGACGAGGCGGAAACCGGTTTTCCTGTCGCAAACCTGCGCAAACGGATATATCCACGTATCATTTCCGCCCAAGA
GGCCAAACAAGAGGCACCCC

Downstream 100 bases:

>100_bases
CGCCGCACAAATGCGGCGTAAAAGATTCCTGTAGCACAGGGTAACTGACAGGCCGCCGATGAGCGGCATTTGAGAAAGCA
TGGGTACAATGAAAACCAAC

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLQTMKTLTLIPARLGSTRLPNKPLADICGKPMIVHVADRAAAAKLGRTVIATDSEEIFKVVAAHGHEAIMTRGDHESGS
DRIYEALAKLDPSGEIDAVVNVQGDLPTIDPDTIRRALLPLEDGPADIATLGVEITVEEEKTNPNVVKIVGSPLAGNRRL
RALYFTRATAPYGEGPLYHHIGLYAYRRSALERFVKLGPSPLEKREKLEQLRALEAGMRIDVEIVKTVPLGVDTQADLDR
ARTFCSQAGTI

Sequences:

>Translated_251_residues
MLQTMKTLTLIPARLGSTRLPNKPLADICGKPMIVHVADRAAAAKLGRTVIATDSEEIFKVVAAHGHEAIMTRGDHESGS
DRIYEALAKLDPSGEIDAVVNVQGDLPTIDPDTIRRALLPLEDGPADIATLGVEITVEEEKTNPNVVKIVGSPLAGNRRL
RALYFTRATAPYGEGPLYHHIGLYAYRRSALERFVKLGPSPLEKREKLEQLRALEAGMRIDVEIVKTVPLGVDTQADLDR
ARTFCSQAGTI
>Mature_251_residues
MLQTMKTLTLIPARLGSTRLPNKPLADICGKPMIVHVADRAAAAKLGRTVIATDSEEIFKVVAAHGHEAIMTRGDHESGS
DRIYEALAKLDPSGEIDAVVNVQGDLPTIDPDTIRRALLPLEDGPADIATLGVEITVEEEKTNPNVVKIVGSPLAGNRRL
RALYFTRATAPYGEGPLYHHIGLYAYRRSALERFVKLGPSPLEKREKLEQLRALEAGMRIDVEIVKTVPLGVDTQADLDR
ARTFCSQAGTI

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=250, Percent_Identity=44.4, Blast_Score=181, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_BRUA1 (B2S7U2)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001934070.1
- ProteinModelPortal:   B2S7U2
- GeneID:   6327161
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I00340
- HOGENOM:   HBG637773
- OMA:   IIPARLK
- ProtClustDB:   PRK05450
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 27305; Mature: 27305

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQTMKTLTLIPARLGSTRLPNKPLADICGKPMIVHVADRAAAAKLGRTVIATDSEEIFK
CCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEECCHHHHHHHCCEEEECCHHHHHH
VVAAHGHEAIMTRGDHESGSDRIYEALAKLDPSGEIDAVVNVQGDLPTIDPDTIRRALLP
HHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHCC
LEDGPADIATLGVEITVEEEKTNPNVVKIVGSPLAGNRRLRALYFTRATAPYGEGPLYHH
CCCCCCCEEEEEEEEEEECCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCHHH
IGLYAYRRSALERFVKLGPSPLEKREKLEQLRALEAGMRIDVEIVKTVPLGVDTQADLDR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHH
ARTFCSQAGTI
HHHHHHHCCCC
>Mature Secondary Structure
MLQTMKTLTLIPARLGSTRLPNKPLADICGKPMIVHVADRAAAAKLGRTVIATDSEEIFK
CCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEECCHHHHHHHCCEEEECCHHHHHH
VVAAHGHEAIMTRGDHESGSDRIYEALAKLDPSGEIDAVVNVQGDLPTIDPDTIRRALLP
HHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHCC
LEDGPADIATLGVEITVEEEKTNPNVVKIVGSPLAGNRRLRALYFTRATAPYGEGPLYHH
CCCCCCCEEEEEEEEEEECCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCHHH
IGLYAYRRSALERFVKLGPSPLEKREKLEQLRALEAGMRIDVEIVKTVPLGVDTQADLDR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHH
ARTFCSQAGTI
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA