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The map label for this gene is hpaI [H]

Identifier: 225686900

GI number: 225686900

Start: 1135033

End: 1135842

Strand: Reverse

Name: hpaI [H]

Synonym: BMEA_B1151

Alternate gene names: 225686900

Gene position: 1135842-1135033 (Counterclockwise)

Preceding gene: 225686901

Following gene: 225686893

Centisome position: 95.81

GC content: 61.11

Gene sequence:

>810_bases
ATGCCTGCTCCTCGAAATCAGTTCAAGGCCGCGCTCAAGCAGGGCCGCGCCCAGATCGGCCTGTGGCAGGCGCTCGCCAA
TCCCTATACGGTCGAAATCTGTGCCGGAGCCGGATATGACTGGCTGCTGCTCGATGGCGAACATGCGCCCAATGATGTGC
CTCTTCTGGTTTCACAGCTTCAGGCCATGACAGGTTCAGCCTCGCATCCGGTCGTGCGGCCGCCTGTCGGCGAAACGTGG
ATCGTCAAGCAATTGCTCGATATCGGCGCGCAGACCCTGCTGATCCCCATGATTGAAAGCCGCGAACAGGCCGAAGCCAT
GGTAAAAGCCATGCGCTATCCGCCGCATGGGGTGCGTGGTGTCGGTTCCGCTCTGGCGCGGGCTTCTGCCTTCAACCGTA
TCCCCGATTATCTACAGACGGCCAATGACGAGGTTTGTCTTCTGTTGCAGGTGGAAAGCCGCGCGGGGCTTGCCAATCTT
GATGCAATTGCCACGACCGAAGGCGTGGACGGCGTCTTCATCGGCCCTGCCGATCTTGCTGCCGATATGGGGCATCTGGG
CAAACCGGGCGCGCAAGAGGTGCAGGACGCTGTGCGCGACGCACTTCAACGCATCATCGGGCACGGCAAGGCGGCGGGCA
TTCTCACGGGCGACCTTTCGCTTGCCCGTCATTATCTCGACCTTGGCGCAACCTTTGTCGCCATCGGCAATGATGTGACC
TTGCTCGCGAATGCCACCACTAGGCTGCTTGCCGACTTCAAGACCACTGAGCCGGGCAGGCCAGCCAGCCCGGACGCAAG
GGTCTATTGA

Upstream 100 bases:

>100_bases
ATTTGGACGCACAAAAATCACTCTAACTTTCTGAACTTACGCATCGTGCTTTCCAAAAATCGATTTCGATTTTTGGGCTG
GTGCTGAAAGGACATTTCTG

Downstream 100 bases:

>100_bases
GCCTGACAGGCCGCCTGTTTCATTTCGGCAAGGTGAAAAGTGCAGTTCCAGACAAAAGGAAGTGCAGGACCAGAGGGGTG
CCCGTTGAGGCCGGGTTGGC

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MPAPRNQFKAALKQGRAQIGLWQALANPYTVEICAGAGYDWLLLDGEHAPNDVPLLVSQLQAMTGSASHPVVRPPVGETW
IVKQLLDIGAQTLLIPMIESREQAEAMVKAMRYPPHGVRGVGSALARASAFNRIPDYLQTANDEVCLLLQVESRAGLANL
DAIATTEGVDGVFIGPADLAADMGHLGKPGAQEVQDAVRDALQRIIGHGKAAGILTGDLSLARHYLDLGATFVAIGNDVT
LLANATTRLLADFKTTEPGRPASPDARVY

Sequences:

>Translated_269_residues
MPAPRNQFKAALKQGRAQIGLWQALANPYTVEICAGAGYDWLLLDGEHAPNDVPLLVSQLQAMTGSASHPVVRPPVGETW
IVKQLLDIGAQTLLIPMIESREQAEAMVKAMRYPPHGVRGVGSALARASAFNRIPDYLQTANDEVCLLLQVESRAGLANL
DAIATTEGVDGVFIGPADLAADMGHLGKPGAQEVQDAVRDALQRIIGHGKAAGILTGDLSLARHYLDLGATFVAIGNDVT
LLANATTRLLADFKTTEPGRPASPDARVY
>Mature_268_residues
PAPRNQFKAALKQGRAQIGLWQALANPYTVEICAGAGYDWLLLDGEHAPNDVPLLVSQLQAMTGSASHPVVRPPVGETWI
VKQLLDIGAQTLLIPMIESREQAEAMVKAMRYPPHGVRGVGSALARASAFNRIPDYLQTANDEVCLLLQVESRAGLANLD
AIATTEGVDGVFIGPADLAADMGHLGKPGAQEVQDAVRDALQRIIGHGKAAGILTGDLSLARHYLDLGATFVAIGNDVTL
LANATTRLLADFKTTEPGRPASPDARVY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=249, Percent_Identity=50.6024096385542, Blast_Score=261, Evalue=4e-71,
Organism=Escherichia coli, GI1788578, Length=254, Percent_Identity=50.7874015748031, Blast_Score=254, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 28527; Mature: 28396

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPRNQFKAALKQGRAQIGLWQALANPYTVEICAGAGYDWLLLDGEHAPNDVPLLVSQL
CCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHHH
QAMTGSASHPVVRPPVGETWIVKQLLDIGAQTLLIPMIESREQAEAMVKAMRYPPHGVRG
HHHCCCCCCCEECCCCCHHHHHHHHHHCCHHHEEHHHHHCHHHHHHHHHHHCCCCCCHHH
VGSALARASAFNRIPDYLQTANDEVCLLLQVESRAGLANLDAIATTEGVDGVFIGPADLA
HHHHHHHHHHHHHCHHHHHCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCEEEECHHHHH
ADMGHLGKPGAQEVQDAVRDALQRIIGHGKAAGILTGDLSLARHYLDLGATFVAIGNDVT
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHCCEEEEECCCEE
LLANATTRLLADFKTTEPGRPASPDARVY
EEECHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PAPRNQFKAALKQGRAQIGLWQALANPYTVEICAGAGYDWLLLDGEHAPNDVPLLVSQL
CCCHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHHH
QAMTGSASHPVVRPPVGETWIVKQLLDIGAQTLLIPMIESREQAEAMVKAMRYPPHGVRG
HHHCCCCCCCEECCCCCHHHHHHHHHHCCHHHEEHHHHHCHHHHHHHHHHHCCCCCCHHH
VGSALARASAFNRIPDYLQTANDEVCLLLQVESRAGLANLDAIATTEGVDGVFIGPADLA
HHHHHHHHHHHHHCHHHHHCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCEEEECHHHHH
ADMGHLGKPGAQEVQDAVRDALQRIIGHGKAAGILTGDLSLARHYLDLGATFVAIGNDVT
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHCCEEEEECCCEE
LLANATTRLLADFKTTEPGRPASPDARVY
EEECHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA