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The map label for this gene is purR [H]

Identifier: 225686740

GI number: 225686740

Start: 949615

End: 950634

Strand: Reverse

Name: purR [H]

Synonym: BMEA_B0968

Alternate gene names: 225686740

Gene position: 950634-949615 (Counterclockwise)

Preceding gene: 225686741

Following gene: 225686739

Centisome position: 80.19

GC content: 61.18

Gene sequence:

>1020_bases
TTGGCCCAGAAAATTAAGCTCTCGACCATCGCTGACGCGCTCGGTGTTTCGACCGCCACCGTTTCGCTGGCATTGCGCGA
TAGTCCGCTGGTTGCCGACCAGACCCGTGAGAAGATCAAGGAACATGCGCGCGCCATCGGTTATATCTATAACCGCCGGG
CGGCGAGCCTTCGTACCTCGCGTTCGGGCATTGTCGGCGTCGTCGTGCGGGACATCATGAACCCGTTTTTCGCGGAAATT
CTCAAATCCATCGAAACCGAGCTCGACCGTTCGCGCCAGACTTTCATCCTGTCCAACCATTATGACCAGCTGGACAAACA
GCGCACCTTCATGGACACGCTTTTGCAGCTTGGTGCGGATGGCGTCATCATGTCGCCTGCTATCGGCACGCCTGCCGAGG
ATATCCAGCTTGCCGAAGACAATGGCCTGCCGGTTGTGCTCATCGCGCGCAGTGTCGAGGGCGTGCATGCTCCGGTCTTC
CGGGGCGACAATGCCTATGGGATCGGCCTTGCCACCAACCATCTGATTTCGCTTGGCCATACGCGCATTGCCATGATTGG
CGGCACGGACCAGACTTCCACGGGCCGCGACCGTTATCGCGGTTATGTGCAGGCCATGGAAAAGGCCGGGCTTGAGGTCC
GCCCCGACTGGCGCATTCCCGGCCCGCGCACCAAGCAGGGCGGGTTCGAGGTCGCGCCGCAGTTTCTGGCCCTGAAAGAT
AAACCAACGGCGGCGGTCTGCTGGAACGACCTGACGGCCATCGGCCTGATGAACGGCATTGCCCGCGCCGGGCTGGTGCC
GGGCGAGGATATTTCCGTAACCGGCTATGACGATCTTGAGGAGGCGGCGATTGCCACGCCCGCGCTTACGACCGTCTGGA
ACGGCCAGCGCGAGGTGGGGCGCAGGGCTGCCCGTGCGCTGCTCGATCAGTTGAACGGCGTTGAGGTTTCCTCGACACAG
GAACTCATCAGACCGGAACTGCATATCCGCCAATCGACATCCAAGCCGAAACACGCTTGA

Upstream 100 bases:

>100_bases
TGCAATGGCATAACCGTTCGCAGGATGAACGGCTGCGCTTCCCTTGCCCGGGGCGCGGCTGGCGTTTCGGACTGCGCACG
GACCGTTTTGGGGAGGCGAC

Downstream 100 bases:

>100_bases
TTCTTCCGCCCCATCCGTCAATCCTGCCGGACGGCTCCCCGGAGTGATTTTGAATTGCTGGAATTGCTCGTGAGATGACG
CACCTATGACCAAACGTGAT

Product: HTH-type transcriptional repressor PurR

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MAQKIKLSTIADALGVSTATVSLALRDSPLVADQTREKIKEHARAIGYIYNRRAASLRTSRSGIVGVVVRDIMNPFFAEI
LKSIETELDRSRQTFILSNHYDQLDKQRTFMDTLLQLGADGVIMSPAIGTPAEDIQLAEDNGLPVVLIARSVEGVHAPVF
RGDNAYGIGLATNHLISLGHTRIAMIGGTDQTSTGRDRYRGYVQAMEKAGLEVRPDWRIPGPRTKQGGFEVAPQFLALKD
KPTAAVCWNDLTAIGLMNGIARAGLVPGEDISVTGYDDLEEAAIATPALTTVWNGQREVGRRAARALLDQLNGVEVSSTQ
ELIRPELHIRQSTSKPKHA

Sequences:

>Translated_339_residues
MAQKIKLSTIADALGVSTATVSLALRDSPLVADQTREKIKEHARAIGYIYNRRAASLRTSRSGIVGVVVRDIMNPFFAEI
LKSIETELDRSRQTFILSNHYDQLDKQRTFMDTLLQLGADGVIMSPAIGTPAEDIQLAEDNGLPVVLIARSVEGVHAPVF
RGDNAYGIGLATNHLISLGHTRIAMIGGTDQTSTGRDRYRGYVQAMEKAGLEVRPDWRIPGPRTKQGGFEVAPQFLALKD
KPTAAVCWNDLTAIGLMNGIARAGLVPGEDISVTGYDDLEEAAIATPALTTVWNGQREVGRRAARALLDQLNGVEVSSTQ
ELIRPELHIRQSTSKPKHA
>Mature_338_residues
AQKIKLSTIADALGVSTATVSLALRDSPLVADQTREKIKEHARAIGYIYNRRAASLRTSRSGIVGVVVRDIMNPFFAEIL
KSIETELDRSRQTFILSNHYDQLDKQRTFMDTLLQLGADGVIMSPAIGTPAEDIQLAEDNGLPVVLIARSVEGVHAPVFR
GDNAYGIGLATNHLISLGHTRIAMIGGTDQTSTGRDRYRGYVQAMEKAGLEVRPDWRIPGPRTKQGGFEVAPQFLALKDK
PTAAVCWNDLTAIGLMNGIARAGLVPGEDISVTGYDDLEEAAIATPALTTVWNGQREVGRRAARALLDQLNGVEVSSTQE
LIRPELHIRQSTSKPKHA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=320, Percent_Identity=32.5, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787906, Length=290, Percent_Identity=31.7241379310345, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787948, Length=325, Percent_Identity=28.6153846153846, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1790194, Length=306, Percent_Identity=28.1045751633987, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789202, Length=290, Percent_Identity=30, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788474, Length=291, Percent_Identity=28.8659793814433, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=27.4626865671642, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787580, Length=313, Percent_Identity=26.1980830670927, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48994940, Length=313, Percent_Identity=27.7955271565495, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789068, Length=285, Percent_Identity=25.6140350877193, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790715, Length=312, Percent_Identity=27.5641025641026, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786268, Length=316, Percent_Identity=21.8354430379747, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790689, Length=338, Percent_Identity=23.6686390532544, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36843; Mature: 36711

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKIKLSTIADALGVSTATVSLALRDSPLVADQTREKIKEHARAIGYIYNRRAASLRTS
CCCEEEHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RSGIVGVVVRDIMNPFFAEILKSIETELDRSRQTFILSNHYDQLDKQRTFMDTLLQLGAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCC
GVIMSPAIGTPAEDIQLAEDNGLPVVLIARSVEGVHAPVFRGDNAYGIGLATNHLISLGH
CEEECCCCCCCHHHCEEECCCCCEEEEEEECCCCCCCCEEECCCCEEECHHHHHHHHCCC
TRIAMIGGTDQTSTGRDRYRGYVQAMEKAGLEVRPDWRIPGPRTKQGGFEVAPQFLALKD
EEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHCCHHEEECC
KPTAAVCWNDLTAIGLMNGIARAGLVPGEDISVTGYDDLEEAAIATPALTTVWNGQREVG
CCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHCCCHHHHHCCCHHHHH
RRAARALLDQLNGVEVSSTQELIRPELHIRQSTSKPKHA
HHHHHHHHHHHCCCCCCCHHHHHCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
AQKIKLSTIADALGVSTATVSLALRDSPLVADQTREKIKEHARAIGYIYNRRAASLRTS
CCEEEHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RSGIVGVVVRDIMNPFFAEILKSIETELDRSRQTFILSNHYDQLDKQRTFMDTLLQLGAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCC
GVIMSPAIGTPAEDIQLAEDNGLPVVLIARSVEGVHAPVFRGDNAYGIGLATNHLISLGH
CEEECCCCCCCHHHCEEECCCCCEEEEEEECCCCCCCCEEECCCCEEECHHHHHHHHCCC
TRIAMIGGTDQTSTGRDRYRGYVQAMEKAGLEVRPDWRIPGPRTKQGGFEVAPQFLALKD
EEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHCCHHEEECC
KPTAAVCWNDLTAIGLMNGIARAGLVPGEDISVTGYDDLEEAAIATPALTTVWNGQREVG
CCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHCCCHHHHHCCCHHHHH
RRAARALLDQLNGVEVSSTQELIRPELHIRQSTSKPKHA
HHHHHHHHHHHCCCCCCCHHHHHCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]