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The map label for this gene is appA [H]

Identifier: 225686553

GI number: 225686553

Start: 746553

End: 748166

Strand: Reverse

Name: appA [H]

Synonym: BMEA_B0765

Alternate gene names: 225686553

Gene position: 748166-746553 (Counterclockwise)

Preceding gene: 225686556

Following gene: 225686552

Centisome position: 63.11

GC content: 57.25

Gene sequence:

>1614_bases
ATGTCTGGGTTTGTTGCGTCGCGCAGGGCGTTTCTCGTTGGCTCGACCTCTCTGGCGGCGCTCGCATTCTGGCCAAAAGC
TACATGGGCAGCGCAAGAAGTGCAGAAGGGTGGGCGGCTGGTTGTGGCAGCGGATTCCGAGCCACGCAATCTGAACCCGG
CCATTATCGCTTCCAACGGCGTATTTTTTGTGGCGAGCAAGGTGATCGAGCCTCTGGCGGAGGCTGCCTATGAAGGGCAA
GGCGGTCTTGCGCCGCGTCTTGCCACAAGCTGGGAAGGCTCTGCCGACGGCCTTACAGCCACTTTTCGCTTGCGTGAAGG
CGTCAACTGGCACGATGGCAAGCCGTTTACTTCAGCCGATGTCGCCTTTTCCGCGATGGAAGTCTGGAAGCCACTGCAAA
ACCTTGGCCGTGTCGTGTTCAAATCGCTGGAAAAGGTCGAGACGCCGGACGAACATACCGCAATCTTCCATTTTTCCGAG
CCAACGCCATTTCAGCTCATCCGCAATGCGCTGCCTGCGCTGACCGCCGTTTTGCCAAAGCATGTCTTTGCCGGCACCGA
TATTGCCGCCAATCCGGCAAACGAAAAGCTCGCCGGCACGGGGCCGTTCAAATATGCAGAACACAAGACGGGCGAATATT
ATCGGCTGGTGAAAAATGCGGACTATTGGGGCGAGGGTGAGCCCTATCTGGATGAAGTAATCTACCGTGTGCTGCCGGAT
CGTGCGGCCGCGGCTGATGCGCTGGAAGCCGGGGAGATCGATCTTGCAGCTTTTTCCGCAGTGCCGCTTGCCGATCTCGA
CCGTATATCGAAAGTGCCGGGCCTTGCCGTTATTTCCAAAGGCTATGAAGGGCTGACCTATCAGCTTGTGGTGGAAATCA
ATCACCGTCGCAAGGAACTGGCAGACCTGAAGGTACGCCAGGCAATCGCCCATGCCATCGACCGCGATTTCGTGGTGAAG
ACGATTTTCCAGGGGTTTGCCGTGCCCTCGACCGGGCCGGTGCCGCGCTATGACAGCCAGTTCTATACGGATGATGTGCC
GACCTATCCCTTTGATGTGAAAAAGGCGAACGCACTTTTGGATGAAGCGGGCTACAGGCGCGGCAAGGACGGAATGCGCT
TTGCGCTCAAGCTTCTGCCTGCACCCTATTTCAACGAGACGAAGCAGTTCGGTGATTATCTGCGGCAGGCGCTTGCCGCC
ATCGGTATCGATGCGCACATCGTCAACAATGATGCCGCCGCGCATCAGAAGGCAGTCTATACGGATCACGATTTCGATCT
CGCCATCGCGCCGACCGTCTATCGCGGCGATCCGGCCATTTCGACCACCATTCTGGTGGAAAGCGGCATTCCGGCGGGTG
TTCCTTTCTCCAATCAGGGCGGCTATGCCAATGCCGAACTCGATGCATTGATTGCCAAAGCCGCAAGCACGACCGATGAA
AAGGAACGCACCGCGCTCTACCAAAAATTCCAGCAGATGGTGGAAGCAGACCTGCCGCTCATCAATGTCGCGGAATGGGG
GTTCATTACGGTTGCCAGCGAGCGCGTGAAAAATGTTTCCAACAATCCGCGCTGGGCGGTTTCCAACTGGGCTGACACAT
GGCTGGAACCATGA

Upstream 100 bases:

>100_bases
GATTTTCGATATTTATTGTCGCGGCAACGGATTTGGCCCTGCCATTTTGAGACGGCTGATATAGAAATAGGCCGTATCGA
AGAAAAATGGGGCGGTAAGC

Downstream 100 bases:

>100_bases
TGAAGCAACCGGATTGATTGTATCTTGCTGACGTGAAACAGATTCTGGCCCTTTTGCGAAAACGTCTGGTTGGCAGCATG
GTGGTGCTGCTGATTGTCGT

Product: family 5 extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MSGFVASRRAFLVGSTSLAALAFWPKATWAAQEVQKGGRLVVAADSEPRNLNPAIIASNGVFFVASKVIEPLAEAAYEGQ
GGLAPRLATSWEGSADGLTATFRLREGVNWHDGKPFTSADVAFSAMEVWKPLQNLGRVVFKSLEKVETPDEHTAIFHFSE
PTPFQLIRNALPALTAVLPKHVFAGTDIAANPANEKLAGTGPFKYAEHKTGEYYRLVKNADYWGEGEPYLDEVIYRVLPD
RAAAADALEAGEIDLAAFSAVPLADLDRISKVPGLAVISKGYEGLTYQLVVEINHRRKELADLKVRQAIAHAIDRDFVVK
TIFQGFAVPSTGPVPRYDSQFYTDDVPTYPFDVKKANALLDEAGYRRGKDGMRFALKLLPAPYFNETKQFGDYLRQALAA
IGIDAHIVNNDAAAHQKAVYTDHDFDLAIAPTVYRGDPAISTTILVESGIPAGVPFSNQGGYANAELDALIAKAASTTDE
KERTALYQKFQQMVEADLPLINVAEWGFITVASERVKNVSNNPRWAVSNWADTWLEP

Sequences:

>Translated_537_residues
MSGFVASRRAFLVGSTSLAALAFWPKATWAAQEVQKGGRLVVAADSEPRNLNPAIIASNGVFFVASKVIEPLAEAAYEGQ
GGLAPRLATSWEGSADGLTATFRLREGVNWHDGKPFTSADVAFSAMEVWKPLQNLGRVVFKSLEKVETPDEHTAIFHFSE
PTPFQLIRNALPALTAVLPKHVFAGTDIAANPANEKLAGTGPFKYAEHKTGEYYRLVKNADYWGEGEPYLDEVIYRVLPD
RAAAADALEAGEIDLAAFSAVPLADLDRISKVPGLAVISKGYEGLTYQLVVEINHRRKELADLKVRQAIAHAIDRDFVVK
TIFQGFAVPSTGPVPRYDSQFYTDDVPTYPFDVKKANALLDEAGYRRGKDGMRFALKLLPAPYFNETKQFGDYLRQALAA
IGIDAHIVNNDAAAHQKAVYTDHDFDLAIAPTVYRGDPAISTTILVESGIPAGVPFSNQGGYANAELDALIAKAASTTDE
KERTALYQKFQQMVEADLPLINVAEWGFITVASERVKNVSNNPRWAVSNWADTWLEP
>Mature_536_residues
SGFVASRRAFLVGSTSLAALAFWPKATWAAQEVQKGGRLVVAADSEPRNLNPAIIASNGVFFVASKVIEPLAEAAYEGQG
GLAPRLATSWEGSADGLTATFRLREGVNWHDGKPFTSADVAFSAMEVWKPLQNLGRVVFKSLEKVETPDEHTAIFHFSEP
TPFQLIRNALPALTAVLPKHVFAGTDIAANPANEKLAGTGPFKYAEHKTGEYYRLVKNADYWGEGEPYLDEVIYRVLPDR
AAAADALEAGEIDLAAFSAVPLADLDRISKVPGLAVISKGYEGLTYQLVVEINHRRKELADLKVRQAIAHAIDRDFVVKT
IFQGFAVPSTGPVPRYDSQFYTDDVPTYPFDVKKANALLDEAGYRRGKDGMRFALKLLPAPYFNETKQFGDYLRQALAAI
GIDAHIVNNDAAAHQKAVYTDHDFDLAIAPTVYRGDPAISTTILVESGIPAGVPFSNQGGYANAELDALIAKAASTTDEK
ERTALYQKFQQMVEADLPLINVAEWGFITVASERVKNVSNNPRWAVSNWADTWLEP

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=520, Percent_Identity=27.3076923076923, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787052, Length=449, Percent_Identity=27.8396436525612, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1787762, Length=498, Percent_Identity=26.5060240963855, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1789887, Length=509, Percent_Identity=25.3438113948919, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1787495, Length=506, Percent_Identity=23.7154150197628, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1789397, Length=491, Percent_Identity=24.6435845213849, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87081878, Length=519, Percent_Identity=25.0481695568401, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787551, Length=448, Percent_Identity=22.3214285714286, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58634; Mature: 58502

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFVASRRAFLVGSTSLAALAFWPKATWAAQEVQKGGRLVVAADSEPRNLNPAIIASNG
CCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCC
VFFVASKVIEPLAEAAYEGQGGLAPRLATSWEGSADGLTATFRLREGVNWHDGKPFTSAD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHH
VAFSAMEVWKPLQNLGRVVFKSLEKVETPDEHTAIFHFSEPTPFQLIRNALPALTAVLPK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
HVFAGTDIAANPANEKLAGTGPFKYAEHKTGEYYRLVKNADYWGEGEPYLDEVIYRVLPD
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCC
RAAAADALEAGEIDLAAFSAVPLADLDRISKVPGLAVISKGYEGLTYQLVVEINHRRKEL
HHHHHHHHCCCCEEEHHHCCCCHHHHHHHHCCCCCEEECCCCCCCEEEEEEEECHHHHHH
ADLKVRQAIAHAIDRDFVVKTIFQGFAVPSTGPVPRYDSQFYTDDVPTYPFDVKKANALL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
DEAGYRRGKDGMRFALKLLPAPYFNETKQFGDYLRQALAAIGIDAHIVNNDAAAHQKAVY
HHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCEE
TDHDFDLAIAPTVYRGDPAISTTILVESGIPAGVPFSNQGGYANAELDALIAKAASTTDE
CCCCCCEEECCEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCH
KERTALYQKFQQMVEADLPLINVAEWGFITVASERVKNVSNNPRWAVSNWADTWLEP
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEHHHHHCCCCCCCEEECCCHHHCCCC
>Mature Secondary Structure 
SGFVASRRAFLVGSTSLAALAFWPKATWAAQEVQKGGRLVVAADSEPRNLNPAIIASNG
CCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCC
VFFVASKVIEPLAEAAYEGQGGLAPRLATSWEGSADGLTATFRLREGVNWHDGKPFTSAD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHH
VAFSAMEVWKPLQNLGRVVFKSLEKVETPDEHTAIFHFSEPTPFQLIRNALPALTAVLPK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
HVFAGTDIAANPANEKLAGTGPFKYAEHKTGEYYRLVKNADYWGEGEPYLDEVIYRVLPD
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCC
RAAAADALEAGEIDLAAFSAVPLADLDRISKVPGLAVISKGYEGLTYQLVVEINHRRKEL
HHHHHHHHCCCCEEEHHHCCCCHHHHHHHHCCCCCEEECCCCCCCEEEEEEEECHHHHHH
ADLKVRQAIAHAIDRDFVVKTIFQGFAVPSTGPVPRYDSQFYTDDVPTYPFDVKKANALL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
DEAGYRRGKDGMRFALKLLPAPYFNETKQFGDYLRQALAAIGIDAHIVNNDAAAHQKAVY
HHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCEE
TDHDFDLAIAPTVYRGDPAISTTILVESGIPAGVPFSNQGGYANAELDALIAKAASTTDE
CCCCCCEEECCEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCH
KERTALYQKFQQMVEADLPLINVAEWGFITVASERVKNVSNNPRWAVSNWADTWLEP
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEHHHHHCCCCCCCEEECCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]