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The map label for this gene is iolD [H]

Identifier: 225686487

GI number: 225686487

Start: 673558

End: 675381

Strand: Direct

Name: iolD [H]

Synonym: BMEA_B0689

Alternate gene names: 225686487

Gene position: 673558-675381 (Clockwise)

Preceding gene: 225686486

Following gene: 225686488

Centisome position: 56.82

GC content: 62.61

Gene sequence:

>1824_bases
ATGAAAACCGTTCGACTGACCGCCGCGCAGGCACTGGTGCGCTATCTGGCCAACCAGATGACGCCGGAGGGCGACACATT
CATCGCGGGCGTCTGGGCCATTTTCGGGCACGGCAATGTGGCGGGGCTGGGCGAGGCGCTCTACGGCGCTCGCGAGCATA
TGCCCACCTGGCGCGGCCATAACGAGCAGACCATGGCCCATGCGGCCATCGCCTATACCAAGCAGCTTGGCCGCAGGCGC
GCCTGCGCCGTCACCTCCTCCATCGGCCCCGGCGCAACCAATATGGTGACAGCGGCAGCACTTGCCCATGTGAACCGCCT
GCCCGTTCTGTTCGTTCCGGGCGATGTCTTCGCCAATCGCGGTCCCGATCCAGTTCTCCAGCAGATCGAGGATTTCAACG
ACGGCACCATGACCGTCAACGACTGTTTCCGCCCGGTAAGCCGTTATTTCGACCGTATCACCCGGCCGGAACAGCTTCTC
ACCGCCCTGCCTCGCGCCTTTCGCACCATGACCGACCCCGCCGATTGCGGCCCGGTGACGCTCGCCTTCTGTCAGGATGT
GCAGGCGCAAGCCTATGACTGGCCGGAAGAATTCTTCACGCCCAAGGTCTGGCACTGGCGCCGCCCGCCGCCGGACGAGC
GGGAACTGGAAACCGCTGCCGCCGCCATCAAAACCGCGCTGAAGCCCGTCATTGTGGCGGGCGGCGGCGTGCATTATTCC
GGCGCGCATGAGGTGCTGCGCGCTTTTGTAGAAACACATGGCATCCCCGTTATTGAAACACAGGCGGGCAAATCCGCACT
GCCATGGGACCATCCGCTGAATTTCGGTCCTGTCGGCGTGACCGGCGCTGACAGCGCCAATGCGGTTGCGGCAGAAGCCG
ATCTGGTGATCGGTGTCGGCACACGCTTTCAGGACTTCACCACCGGCTCATGGGCCCTGTTCAGGCATCCGGGGCGCAGG
CTTCTTTCGCTCAATGTACAGCCCTATGACGGAGCCAAGCATAATTCTCTGCCGCTGGTAGCAGATGCGAAGATTGGGCT
TGAGGCACTCTCCGGGGCCATTGGCGCTTACAGACGCCAGGCCGTCGATGCCAGCCTCAAAAACAGATGGTTTGCCGCTG
CCGACCGTTTCACCGCCGCGCCCAGCGACGGCAATGCGCTACCGACCGACATGCAGGTCATCGGTGCGGTGCAGCGGCAA
GCGCGCGAAAATTCCGTTGTCATGTGCGCGGCAGGCACCATGCCGGGTGAGTTGCATCAGCTCTGGAAGGCCGGGCGCCC
CATGTCCTACCACATGGAATATGGCTTTTCCTGCATGGGCTATGAGATTGCCGGCGGCCTTGGCATCAAGATGGCCGAAC
CGGACCGCGACGTCATCGTGATGGTGGGCGACGGTTCCTATATGATGGCCAATTCGGAACTGGCCACCAGCATCATGATG
GGGCAGAAAATCACCGTCGTCGTGACCGACAATCGCGGTTTCGGCTGCATCAGCCGGCTGCAAATGGCGACCGGCGGCGC
GGAATTCAACAATCTGCTCGACCATGCCGCCCATGTGAACCCGTCGCAGATCGATTTTGCCGCGCACGCCGCCGCCATGG
GTGCGGATGCGAAAAAGGTCGGCTCCATCCGCGAACTGGAGGGCGCACTGGCCGAAGCACGCGACAGCCTGCGCACGACC
GTCATTGTTATCGACACCGACCCTTACCCCACGCCGGAAACCGGCGGCTGGTGGTGGGATGTCGCCGTACCGGAAGTCTC
GGAACGCGATGAGGTGCGCGCCGCACGCAAAAACTATGAAGCCCAGATCAAAGAAAGAAACTGA

Upstream 100 bases:

>100_bases
CCATCGCCGAAATGGCCGCGCGCTACCAGAGCCTTTGCGACGTGTGGGACAAGGCGCACGCTTACTGAACACTTTCCCTC
GGGGGAAGGATGGAGGAAAT

Downstream 100 bases:

>100_bases
CCGATGATCCTTTACGGCACCAACCCCATTGCATGGTCAAACGATGACGACCGCAGCCTCGGCGCGCATATCACGCTTGA
TCAATGCCTCGACGAAGCCG

Product: thiamine pyrophosphate protein

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MKTVRLTAAQALVRYLANQMTPEGDTFIAGVWAIFGHGNVAGLGEALYGAREHMPTWRGHNEQTMAHAAIAYTKQLGRRR
ACAVTSSIGPGATNMVTAAALAHVNRLPVLFVPGDVFANRGPDPVLQQIEDFNDGTMTVNDCFRPVSRYFDRITRPEQLL
TALPRAFRTMTDPADCGPVTLAFCQDVQAQAYDWPEEFFTPKVWHWRRPPPDERELETAAAAIKTALKPVIVAGGGVHYS
GAHEVLRAFVETHGIPVIETQAGKSALPWDHPLNFGPVGVTGADSANAVAAEADLVIGVGTRFQDFTTGSWALFRHPGRR
LLSLNVQPYDGAKHNSLPLVADAKIGLEALSGAIGAYRRQAVDASLKNRWFAAADRFTAAPSDGNALPTDMQVIGAVQRQ
ARENSVVMCAAGTMPGELHQLWKAGRPMSYHMEYGFSCMGYEIAGGLGIKMAEPDRDVIVMVGDGSYMMANSELATSIMM
GQKITVVVTDNRGFGCISRLQMATGGAEFNNLLDHAAHVNPSQIDFAAHAAAMGADAKKVGSIRELEGALAEARDSLRTT
VIVIDTDPYPTPETGGWWWDVAVPEVSERDEVRAARKNYEAQIKERN

Sequences:

>Translated_607_residues
MKTVRLTAAQALVRYLANQMTPEGDTFIAGVWAIFGHGNVAGLGEALYGAREHMPTWRGHNEQTMAHAAIAYTKQLGRRR
ACAVTSSIGPGATNMVTAAALAHVNRLPVLFVPGDVFANRGPDPVLQQIEDFNDGTMTVNDCFRPVSRYFDRITRPEQLL
TALPRAFRTMTDPADCGPVTLAFCQDVQAQAYDWPEEFFTPKVWHWRRPPPDERELETAAAAIKTALKPVIVAGGGVHYS
GAHEVLRAFVETHGIPVIETQAGKSALPWDHPLNFGPVGVTGADSANAVAAEADLVIGVGTRFQDFTTGSWALFRHPGRR
LLSLNVQPYDGAKHNSLPLVADAKIGLEALSGAIGAYRRQAVDASLKNRWFAAADRFTAAPSDGNALPTDMQVIGAVQRQ
ARENSVVMCAAGTMPGELHQLWKAGRPMSYHMEYGFSCMGYEIAGGLGIKMAEPDRDVIVMVGDGSYMMANSELATSIMM
GQKITVVVTDNRGFGCISRLQMATGGAEFNNLLDHAAHVNPSQIDFAAHAAAMGADAKKVGSIRELEGALAEARDSLRTT
VIVIDTDPYPTPETGGWWWDVAVPEVSERDEVRAARKNYEAQIKERN
>Mature_607_residues
MKTVRLTAAQALVRYLANQMTPEGDTFIAGVWAIFGHGNVAGLGEALYGAREHMPTWRGHNEQTMAHAAIAYTKQLGRRR
ACAVTSSIGPGATNMVTAAALAHVNRLPVLFVPGDVFANRGPDPVLQQIEDFNDGTMTVNDCFRPVSRYFDRITRPEQLL
TALPRAFRTMTDPADCGPVTLAFCQDVQAQAYDWPEEFFTPKVWHWRRPPPDERELETAAAAIKTALKPVIVAGGGVHYS
GAHEVLRAFVETHGIPVIETQAGKSALPWDHPLNFGPVGVTGADSANAVAAEADLVIGVGTRFQDFTTGSWALFRHPGRR
LLSLNVQPYDGAKHNSLPLVADAKIGLEALSGAIGAYRRQAVDASLKNRWFAAADRFTAAPSDGNALPTDMQVIGAVQRQ
ARENSVVMCAAGTMPGELHQLWKAGRPMSYHMEYGFSCMGYEIAGGLGIKMAEPDRDVIVMVGDGSYMMANSELATSIMM
GQKITVVVTDNRGFGCISRLQMATGGAEFNNLLDHAAHVNPSQIDFAAHAAAMGADAKKVGSIRELEGALAEARDSLRTT
VIVIDTDPYPTPETGGWWWDVAVPEVSERDEVRAARKNYEAQIKERN

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=582, Percent_Identity=26.4604810996564, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1790104, Length=557, Percent_Identity=25.1346499102334, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1786717, Length=512, Percent_Identity=25.78125, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=22.8828828828829, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1788716, Length=523, Percent_Identity=23.1357552581262, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323755, Length=538, Percent_Identity=23.6059479553903, Blast_Score=119, Evalue=1e-27,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 65736; Mature: 65736

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVRLTAAQALVRYLANQMTPEGDTFIAGVWAIFGHGNVAGLGEALYGAREHMPTWRGH
CCEEEHHHHHHHHHHHHHHCCCCCCEEHEEEEHHHCCCCHHHHHHHHHHHHHCCCCCCCC
NEQTMAHAAIAYTKQLGRRRACAVTSSIGPGATNMVTAAALAHVNRLPVLFVPGDVFANR
CHHHHHHHHHHHHHHHCCHHHHEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHCCC
GPDPVLQQIEDFNDGTMTVNDCFRPVSRYFDRITRPEQLLTALPRAFRTMTDPADCGPVT
CCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCH
LAFCQDVQAQAYDWPEEFFTPKVWHWRRPPPDERELETAAAAIKTALKPVIVAGGGVHYS
HHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCEEEC
GAHEVLRAFVETHGIPVIETQAGKSALPWDHPLNFGPVGVTGADSANAVAAEADLVIGVG
CHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECC
TRFQDFTTGSWALFRHPGRRLLSLNVQPYDGAKHNSLPLVADAKIGLEALSGAIGAYRRQ
CCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHH
AVDASLKNRWFAAADRFTAAPSDGNALPTDMQVIGAVQRQARENSVVMCAAGTMPGELHQ
HHHHHHHHCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
LWKAGRPMSYHMEYGFSCMGYEIAGGLGIKMAEPDRDVIVMVGDGSYMMANSELATSIMM
HHHCCCCEEEECCCCCEEECEEEECCCEEEECCCCCCEEEEECCCCEEEECHHHHHHHHC
GQKITVVVTDNRGFGCISRLQMATGGAEFNNLLDHAAHVNPSQIDFAAHAAAMGADAKKV
CCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHH
GSIRELEGALAEARDSLRTTVIVIDTDPYPTPETGGWWWDVAVPEVSERDEVRAARKNYE
CCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCC
AQIKERN
CEEECCC
>Mature Secondary Structure
MKTVRLTAAQALVRYLANQMTPEGDTFIAGVWAIFGHGNVAGLGEALYGAREHMPTWRGH
CCEEEHHHHHHHHHHHHHHCCCCCCEEHEEEEHHHCCCCHHHHHHHHHHHHHCCCCCCCC
NEQTMAHAAIAYTKQLGRRRACAVTSSIGPGATNMVTAAALAHVNRLPVLFVPGDVFANR
CHHHHHHHHHHHHHHHCCHHHHEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHCCC
GPDPVLQQIEDFNDGTMTVNDCFRPVSRYFDRITRPEQLLTALPRAFRTMTDPADCGPVT
CCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCH
LAFCQDVQAQAYDWPEEFFTPKVWHWRRPPPDERELETAAAAIKTALKPVIVAGGGVHYS
HHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCEEEC
GAHEVLRAFVETHGIPVIETQAGKSALPWDHPLNFGPVGVTGADSANAVAAEADLVIGVG
CHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECC
TRFQDFTTGSWALFRHPGRRLLSLNVQPYDGAKHNSLPLVADAKIGLEALSGAIGAYRRQ
CCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHH
AVDASLKNRWFAAADRFTAAPSDGNALPTDMQVIGAVQRQARENSVVMCAAGTMPGELHQ
HHHHHHHHCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
LWKAGRPMSYHMEYGFSCMGYEIAGGLGIKMAEPDRDVIVMVGDGSYMMANSELATSIMM
HHHCCCCEEEECCCCCEEECEEEECCCEEEECCCCCCEEEEECCCCEEEECHHHHHHHHC
GQKITVVVTDNRGFGCISRLQMATGGAEFNNLLDHAAHVNPSQIDFAAHAAAMGADAKKV
CCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHH
GSIRELEGALAEARDSLRTTVIVIDTDPYPTPETGGWWWDVAVPEVSERDEVRAARKNYE
CCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCC
AQIKERN
CEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA