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The map label for this gene is lipB [H]

Identifier: 225686375

GI number: 225686375

Start: 555243

End: 556037

Strand: Direct

Name: lipB [H]

Synonym: BMEA_B0564

Alternate gene names: 225686375

Gene position: 555243-556037 (Clockwise)

Preceding gene: 225686374

Following gene: 225686377

Centisome position: 46.84

GC content: 60.88

Gene sequence:

>795_bases
TTGCCCACTGGCAAATTGCGCCAAAAGCCGCCATATGCTGCCATCATGACAAACAGCCCAGTTACCCCTTCCACTGAAAC
GCAACAGCCCAAACGCTATGCGCGCTTCCTTCCGCAGGAAGCCGATGCGCCGCCGGTGGAGTGGCTCATCACCGAAGGGT
TGACCGACTATGAGGAAGCCCTCGCCTTCATGGAAGCGCGTGTGCAGGCGATCCGCGAAGGCACTGCCAGCGAACTCGTG
TGGCTGGTGGAGCATCCTCCGCTCTATACGGCCGGAACGAGTGCCAACGCCGAAGACCTGCTGACGCCGGACCGCTTTCC
GGTGTTCAACACCGGGCGTGGCGGCGAATATACCTATCATGGTCCCGGCCAGCGCGTTGCCTATGTAATGCTCGACCTGA
AGCGCCGCCGGGAGGATGTGCGGGCCTTCGTGGCGTCACTGGAACAATGGATCATCGAGACGCTGGCTGCGTTCAACATC
AAGGGCGAACGCCGTGAAGACCGCGTTGGCGTCTGGGTCGTGCGGCCTGAAAAGCCGCGCCTTGCCGATGGAAGCATGTG
CGAGGACAAGATTGCCGCTATCGGCATTCGCCTGCGCCGTTGGGTGAGCTTTCACGGCATCGCCATCAATGTAGAGCCGG
ACCTCAGCCATTACGGCGGCATCGTGCCTTGCGGGATTTCCGAGCATGGCGTGACCAGCCTTGTCGATCTTGGCCTGCCC
GTCACGATGGGCGATGTTGATGTCGCGCTCGGCAAGGCTTTTGAAAGCGTGTTCGGACCCCGACAGACGAAATAA

Upstream 100 bases:

>100_bases
GTCCTTATCCAATCGTAGTTGGAAAATGCGACAAAACAAATAGTTAGTGCGGTTCCGACGATTCTGTTAAAATGGGAACC
GCTCTAAATGGCAGGGCATT

Downstream 100 bases:

>100_bases
ATATCATGAGATCCGTCCTGTTTTTTTCTTGCTTTCACGCATGAAGACGTAAAAGCCGGAGCCAATGATGATGGCGATGC
CGAGCCATTTGGATGGCGTT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MPTGKLRQKPPYAAIMTNSPVTPSTETQQPKRYARFLPQEADAPPVEWLITEGLTDYEEALAFMEARVQAIREGTASELV
WLVEHPPLYTAGTSANAEDLLTPDRFPVFNTGRGGEYTYHGPGQRVAYVMLDLKRRREDVRAFVASLEQWIIETLAAFNI
KGERREDRVGVWVVRPEKPRLADGSMCEDKIAAIGIRLRRWVSFHGIAINVEPDLSHYGGIVPCGISEHGVTSLVDLGLP
VTMGDVDVALGKAFESVFGPRQTK

Sequences:

>Translated_264_residues
MPTGKLRQKPPYAAIMTNSPVTPSTETQQPKRYARFLPQEADAPPVEWLITEGLTDYEEALAFMEARVQAIREGTASELV
WLVEHPPLYTAGTSANAEDLLTPDRFPVFNTGRGGEYTYHGPGQRVAYVMLDLKRRREDVRAFVASLEQWIIETLAAFNI
KGERREDRVGVWVVRPEKPRLADGSMCEDKIAAIGIRLRRWVSFHGIAINVEPDLSHYGGIVPCGISEHGVTSLVDLGLP
VTMGDVDVALGKAFESVFGPRQTK
>Mature_263_residues
PTGKLRQKPPYAAIMTNSPVTPSTETQQPKRYARFLPQEADAPPVEWLITEGLTDYEEALAFMEARVQAIREGTASELVW
LVEHPPLYTAGTSANAEDLLTPDRFPVFNTGRGGEYTYHGPGQRVAYVMLDLKRRREDVRAFVASLEQWIIETLAAFNIK
GERREDRVGVWVVRPEKPRLADGSMCEDKIAAIGIRLRRWVSFHGIAINVEPDLSHYGGIVPCGISEHGVTSLVDLGLPV
TMGDVDVALGKAFESVFGPRQTK

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=234, Percent_Identity=32.4786324786325, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI87081767, Length=173, Percent_Identity=43.3526011560694, Blast_Score=138, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6323268, Length=266, Percent_Identity=25.9398496240602, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21355483, Length=229, Percent_Identity=34.4978165938865, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 29332; Mature: 29201

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTGKLRQKPPYAAIMTNSPVTPSTETQQPKRYARFLPQEADAPPVEWLITEGLTDYEEA
CCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHH
LAFMEARVQAIREGTASELVWLVEHPPLYTAGTSANAEDLLTPDRFPVFNTGRGGEYTYH
HHHHHHHHHHHHCCCHHHEEEEEECCCEEECCCCCCHHHCCCCCCCCCEECCCCCCEEEC
GPGQRVAYVMLDLKRRREDVRAFVASLEQWIIETLAAFNIKGERREDRVGVWVVRPEKPR
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
LADGSMCEDKIAAIGIRLRRWVSFHGIAINVEPDLSHYGGIVPCGISEHGVTSLVDLGLP
CCCCCCCHHHHHHHHHHHHHHHEECCEEEEECCCHHHCCCEEECCCCCCCHHHHHHCCCC
VTMGDVDVALGKAFESVFGPRQTK
EEECCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PTGKLRQKPPYAAIMTNSPVTPSTETQQPKRYARFLPQEADAPPVEWLITEGLTDYEEA
CCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHH
LAFMEARVQAIREGTASELVWLVEHPPLYTAGTSANAEDLLTPDRFPVFNTGRGGEYTYH
HHHHHHHHHHHHCCCHHHEEEEEECCCEEECCCCCCHHHCCCCCCCCCEECCCCCCEEEC
GPGQRVAYVMLDLKRRREDVRAFVASLEQWIIETLAAFNIKGERREDRVGVWVVRPEKPR
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
LADGSMCEDKIAAIGIRLRRWVSFHGIAINVEPDLSHYGGIVPCGISEHGVTSLVDLGLP
CCCCCCCHHHHHHHHHHHHHHHEECCEEEEECCCHHHCCCEEECCCCCCCHHHHHHCCCC
VTMGDVDVALGKAFESVFGPRQTK
EEECCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA