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The map label for this gene is ytcB [H]

Identifier: 225686333

GI number: 225686333

Start: 502845

End: 503441

Strand: Direct

Name: ytcB [H]

Synonym: BMEA_B0515

Alternate gene names: 225686333

Gene position: 502845-503441 (Clockwise)

Preceding gene: 225686324

Following gene: 225686334

Centisome position: 42.42

GC content: 59.13

Gene sequence:

>597_bases
ATGAAAGTGCTTTATTTACGTAGCGTTTCCATCCAGGTGAGGCCAAACACCACGGCCACCATTGACGAGGGCAAGCGGTC
TGCCGAAACGCTGTTCCACGATTTCCACCAGCAATATGGTGTCGATATCCGCATTGTCAGGATTTTCAATACCTACGGTC
CGCGCATGCGTCCCGACGACGGCCGTGTGGTGTCGAACTTCATCGTGCAGGCGCTGAAGGGCGAGGACATTACCGTCTAT
GGGGATGGTTCGCAGACGCGCTCCTTCTGTTATGTGGACGATCTGATCGAGGGCTTCCACCGGCTCATGTATAGCCCGCA
CGCCATTCGTGTGCCGGTGAATATCGGCAATCCGGGCGAATTCACAGTCGGCGCCCTGGCAGAGCAGATCATTGCCATGA
CCGGGTCGCGGTCGCGCATCGTTTATTATCCGCTGCCGGTCGATGATCCGCGCCAGCGCCGGCCCGACATCACCGTTGCC
AAGCGCGAACTCGGCTGGGAGCCGACCGTGGCGCTGGCCCAGGGCCTGGAACCGACTATCGCCTATTTCGAGCATCAGTT
GAGAAAGCCGACCGGCAAGCTGGTGGAGGCCGCATAA

Upstream 100 bases:

>100_bases
CAAGCTATCCGTTCAAAAAGGGCTATATCGTGAAGCAGCGTGATGCATTTCCCCGCTGAATGCGCCGAACCTCCCTTATG
AAGAGGGGTTAGCTTGCCGG

Downstream 100 bases:

>100_bases
TGTCCGCGAACAACGTCCTCGTCGTCGGTGGCGCCGGATTCATCGGCAGCCACACGGCCAAGCTTCTGGCCGGGCAGGGC
TATGCACCCGTCGTCTACGA

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MKVLYLRSVSIQVRPNTTATIDEGKRSAETLFHDFHQQYGVDIRIVRIFNTYGPRMRPDDGRVVSNFIVQALKGEDITVY
GDGSQTRSFCYVDDLIEGFHRLMYSPHAIRVPVNIGNPGEFTVGALAEQIIAMTGSRSRIVYYPLPVDDPRQRRPDITVA
KRELGWEPTVALAQGLEPTIAYFEHQLRKPTGKLVEAA

Sequences:

>Translated_198_residues
MKVLYLRSVSIQVRPNTTATIDEGKRSAETLFHDFHQQYGVDIRIVRIFNTYGPRMRPDDGRVVSNFIVQALKGEDITVY
GDGSQTRSFCYVDDLIEGFHRLMYSPHAIRVPVNIGNPGEFTVGALAEQIIAMTGSRSRIVYYPLPVDDPRQRRPDITVA
KRELGWEPTVALAQGLEPTIAYFEHQLRKPTGKLVEAA
>Mature_198_residues
MKVLYLRSVSIQVRPNTTATIDEGKRSAETLFHDFHQQYGVDIRIVRIFNTYGPRMRPDDGRVVSNFIVQALKGEDITVY
GDGSQTRSFCYVDDLIEGFHRLMYSPHAIRVPVNIGNPGEFTVGALAEQIIAMTGSRSRIVYYPLPVDDPRQRRPDITVA
KRELGWEPTVALAQGLEPTIAYFEHQLRKPTGKLVEAA

Specific function: Two Step Nadp-Dependent Conversion Of Gdp-4-Dehydro-6- Deoxy-D-Mannose To Gdp-Fucose, Involving An Epimerase And A Reductase Reaction. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=169, Percent_Identity=53.2544378698225, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI7657641, Length=181, Percent_Identity=28.7292817679558, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1788365, Length=174, Percent_Identity=27.5862068965517, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539532, Length=170, Percent_Identity=51.1764705882353, Blast_Score=170, Evalue=5e-43,
Organism=Drosophila melanogaster, GI21356223, Length=169, Percent_Identity=53.8461538461538, Blast_Score=176, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46

Molecular weight: Translated: 22363; Mature: 22363

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLYLRSVSIQVRPNTTATIDEGKRSAETLFHDFHQQYGVDIRIVRIFNTYGPRMRPDD
CEEEEEEEEEEEECCCCCEECCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
GRVVSNFIVQALKGEDITVYGDGSQTRSFCYVDDLIEGFHRLMYSPHAIRVPVNIGNPGE
CHHHHHHHHHHHCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCC
FTVGALAEQIIAMTGSRSRIVYYPLPVDDPRQRRPDITVAKRELGWEPTVALAQGLEPTI
CHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCCEEEHHHCCCCCHHHHHCCCCHHH
AYFEHQLRKPTGKLVEAA
HHHHHHHCCCCCHHCCCC
>Mature Secondary Structure
MKVLYLRSVSIQVRPNTTATIDEGKRSAETLFHDFHQQYGVDIRIVRIFNTYGPRMRPDD
CEEEEEEEEEEEECCCCCEECCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
GRVVSNFIVQALKGEDITVYGDGSQTRSFCYVDDLIEGFHRLMYSPHAIRVPVNIGNPGE
CHHHHHHHHHHHCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCC
FTVGALAEQIIAMTGSRSRIVYYPLPVDDPRQRRPDITVAKRELGWEPTVALAQGLEPTI
CHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCCEEEHHHCCCCCHHHHHCCCCHHH
AYFEHQLRKPTGKLVEAA
HHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDPglucose

Specific reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]