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The map label for this gene is lpdA [H]

Identifier: 225686319

GI number: 225686319

Start: 485725

End: 487026

Strand: Direct

Name: lpdA [H]

Synonym: BMEA_B0500

Alternate gene names: 225686319

Gene position: 485725-487026 (Clockwise)

Preceding gene: 225686318

Following gene: 225686321

Centisome position: 40.97

GC content: 60.06

Gene sequence:

>1302_bases
GTGCTGGTGGAAAAGACGCGCCTTGGCGGCACCTGCCTCAATGTCGGCTGCATTCCGTCCAAGGCATTGATCCATGCGGC
AGATGAATTTCATTGCCTCACCACTTTCGCGGCCAAAAGCCCGCTTGGCATCACGACGCAAAACCCGGCCATCGATTTCG
CCCGGACGCTGGAATGGAAGGATGGAATCGTCCATCGGCTGAACAGTGGCGTTGCCGGGCTTTTGAAGCGGTCGCGCGTG
CGCATGTTTCAGGGGCAGGCGCGCTTTCAGGATGGCAAGACGGTGCTGGTCGATACGGATACGGGCCGCCAGACCATTCA
TGCTGAAAACATCGTGATCGCAACCGGCTCGGTGCCGGTCGAAATTCAGGCTTTGCCCTTCGGCGGCAATATCATCTCCT
CCACCGAGGCGCTTTCTCTGGAAAAGATACCGGAGCGGCTGGCCGTGGTTGGCGGCGGTTATATCGGGCTTGAAATTGGA
ACGGCCTTCGCCAAACTTGGCTCCAGGGTGATGGTCGTGGAAGCGACCGACCGGATTCTTCCCCAATATGATGCGGAACT
GACCCGCCCCGTAATGGCGCGCCTCAAGACGCTTGGGGTGGAGGTACTGACCGGAACGTCCGCCAAAGGGCTGTCCGCGG
ACGGCAAGGCGCTGGAAATCCGCACGCAGGACGGTGCCGTCAAGGCGATTGAGGCTGACAAGATCCTCGTGACCGTCGGG
CGCAAGCCGCAGACGGATGGCTGGGGCTTAAGCGAAATCCGGCTGGATATGGATGGCCGTTTCATCCGCATCGATGATCG
CTGCCGCACCTCCATGCGCGGGATTTATGCCATTGGCGACGTCACCGGCGAACCGATGCTAGCGCACCGCGCCATGGCGC
AAGGAGAAATGGTGGCCGAAATAATTGCGGGCGGTAAACATGCCTGGGACAAGCGGTGCATCCCCGCTGTCTGCTTTACC
GACCCGGAAATCGTGACCGTTGGCCTTTCGCTCGATGAAGCGCGCAAGGCCGGGCATAATATCCAGACTGGCCTTTTCCC
GTTTCAGGCCAATGGCCGCGCCATGACGATGGAACGCGATGATGGCATAGTGCGCGTGGTTGCCCGCGCGGACAATCACG
TCATCTTGGGCATTCAGGCAGTCGGAGCCGGTATTTCGGAACTGTCGGCAGCTTTTGCTCAGGCGGTGGAAATGGGTGCA
CGTCTGGAAGATATCGCCGCAACGATCCATGCCCACCCGACCTTGGGCGAAGGCTTTGCCGAGGCCAGCATGAAGGCGCT
TGGCCACGCTCTACATGTTTGA

Upstream 100 bases:

>100_bases
TGACCAGACGAGCGAGATTTCCTGCAAACTTCTGATTATCGGCGGCGGTCCCGGCGGCTATGTATGCGGCATTCGCGCAG
GCCAGCTTGGCATCGATACG

Downstream 100 bases:

>100_bases
TGATCTGCGCTCGTGCCGCGAGACGAAAATGGCGGCACCGGCAACCATAGCCGCCAGCGCAAACCATGTGATGGCATAGG
AAAGGTGGCTGTTGCGAAAC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MLVEKTRLGGTCLNVGCIPSKALIHAADEFHCLTTFAAKSPLGITTQNPAIDFARTLEWKDGIVHRLNSGVAGLLKRSRV
RMFQGQARFQDGKTVLVDTDTGRQTIHAENIVIATGSVPVEIQALPFGGNIISSTEALSLEKIPERLAVVGGGYIGLEIG
TAFAKLGSRVMVVEATDRILPQYDAELTRPVMARLKTLGVEVLTGTSAKGLSADGKALEIRTQDGAVKAIEADKILVTVG
RKPQTDGWGLSEIRLDMDGRFIRIDDRCRTSMRGIYAIGDVTGEPMLAHRAMAQGEMVAEIIAGGKHAWDKRCIPAVCFT
DPEIVTVGLSLDEARKAGHNIQTGLFPFQANGRAMTMERDDGIVRVVARADNHVILGIQAVGAGISELSAAFAQAVEMGA
RLEDIAATIHAHPTLGEGFAEASMKALGHALHV

Sequences:

>Translated_433_residues
MLVEKTRLGGTCLNVGCIPSKALIHAADEFHCLTTFAAKSPLGITTQNPAIDFARTLEWKDGIVHRLNSGVAGLLKRSRV
RMFQGQARFQDGKTVLVDTDTGRQTIHAENIVIATGSVPVEIQALPFGGNIISSTEALSLEKIPERLAVVGGGYIGLEIG
TAFAKLGSRVMVVEATDRILPQYDAELTRPVMARLKTLGVEVLTGTSAKGLSADGKALEIRTQDGAVKAIEADKILVTVG
RKPQTDGWGLSEIRLDMDGRFIRIDDRCRTSMRGIYAIGDVTGEPMLAHRAMAQGEMVAEIIAGGKHAWDKRCIPAVCFT
DPEIVTVGLSLDEARKAGHNIQTGLFPFQANGRAMTMERDDGIVRVVARADNHVILGIQAVGAGISELSAAFAQAVEMGA
RLEDIAATIHAHPTLGEGFAEASMKALGHALHV
>Mature_433_residues
MLVEKTRLGGTCLNVGCIPSKALIHAADEFHCLTTFAAKSPLGITTQNPAIDFARTLEWKDGIVHRLNSGVAGLLKRSRV
RMFQGQARFQDGKTVLVDTDTGRQTIHAENIVIATGSVPVEIQALPFGGNIISSTEALSLEKIPERLAVVGGGYIGLEIG
TAFAKLGSRVMVVEATDRILPQYDAELTRPVMARLKTLGVEVLTGTSAKGLSADGKALEIRTQDGAVKAIEADKILVTVG
RKPQTDGWGLSEIRLDMDGRFIRIDDRCRTSMRGIYAIGDVTGEPMLAHRAMAQGEMVAEIIAGGKHAWDKRCIPAVCFT
DPEIVTVGLSLDEARKAGHNIQTGLFPFQANGRAMTMERDDGIVRVVARADNHVILGIQAVGAGISELSAAFAQAVEMGA
RLEDIAATIHAHPTLGEGFAEASMKALGHALHV

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=444, Percent_Identity=37.1621621621622, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI50301238, Length=433, Percent_Identity=30.4849884526559, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI22035672, Length=434, Percent_Identity=28.5714285714286, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI148277065, Length=435, Percent_Identity=24.1379310344828, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI148277071, Length=435, Percent_Identity=24.1379310344828, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI33519430, Length=435, Percent_Identity=24.1379310344828, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI33519428, Length=435, Percent_Identity=24.1379310344828, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI33519426, Length=435, Percent_Identity=24.1379310344828, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1786307, Length=421, Percent_Identity=37.29216152019, Blast_Score=292, Evalue=2e-80,
Organism=Escherichia coli, GI1789915, Length=427, Percent_Identity=29.9765807962529, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI87082354, Length=434, Percent_Identity=27.6497695852535, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87081717, Length=428, Percent_Identity=26.4018691588785, Blast_Score=145, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=440, Percent_Identity=37.2727272727273, Blast_Score=268, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI71983419, Length=433, Percent_Identity=28.8683602771363, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=433, Percent_Identity=28.8683602771363, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17557007, Length=443, Percent_Identity=26.6365688487585, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=451, Percent_Identity=23.9467849223947, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321091, Length=452, Percent_Identity=37.1681415929204, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6325166, Length=437, Percent_Identity=28.604118993135, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325240, Length=442, Percent_Identity=28.2805429864253, Blast_Score=143, Evalue=5e-35,
Organism=Drosophila melanogaster, GI21358499, Length=437, Percent_Identity=36.6132723112128, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24640549, Length=435, Percent_Identity=27.5862068965517, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24640553, Length=435, Percent_Identity=27.5862068965517, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24640551, Length=434, Percent_Identity=27.4193548387097, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI17737741, Length=440, Percent_Identity=26.1363636363636, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 46183; Mature: 46183

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVEKTRLGGTCLNVGCIPSKALIHAADEFHCLTTFAAKSPLGITTQNPAIDFARTLEWK
CCCCCCCCCCEEEEECCCCCHHHEECCCCCEEEEHHHCCCCCCCCCCCCCHHHHHHCCHH
DGIVHRLNSGVAGLLKRSRVRMFQGQARFQDGKTVLVDTDTGRQTIHAENIVIATGSVPV
HHHHHHHCCCHHHHHHHHHHHHHCCCCEECCCCEEEEECCCCCEEEEECEEEEEECCCCE
EIQALPFGGNIISSTEALSLEKIPERLAVVGGGYIGLEIGTAFAKLGSRVMVVEATDRIL
EEEEEECCCCEECCCHHHHHHHHHHHHEEECCCEEEEEHHHHHHHHCCEEEEEEECCCCC
PQYDAELTRPVMARLKTLGVEVLTGTSAKGLSADGKALEIRTQDGAVKAIEADKILVTVG
CCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEEEECCCCEEEEECCEEEEEEC
RKPQTDGWGLSEIRLDMDGRFIRIDDRCRTSMRGIYAIGDVTGEPMLAHRAMAQGEMVAE
CCCCCCCCCCEEEEECCCCCEEEECHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHH
IIAGGKHAWDKRCIPAVCFTDPEIVTVGLSLDEARKAGHNIQTGLFPFQANGRAMTMERD
HHCCCCCCCCCCCCCEEEECCCCEEEEECCHHHHHHCCCCCCCCEEEEECCCEEEEEECC
DGIVRVVARADNHVILGIQAVGAGISELSAAFAQAVEMGARLEDIAATIHAHPTLGEGFA
CCEEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHH
EASMKALGHALHV
HHHHHHHHHHHCC
>Mature Secondary Structure
MLVEKTRLGGTCLNVGCIPSKALIHAADEFHCLTTFAAKSPLGITTQNPAIDFARTLEWK
CCCCCCCCCCEEEEECCCCCHHHEECCCCCEEEEHHHCCCCCCCCCCCCCHHHHHHCCHH
DGIVHRLNSGVAGLLKRSRVRMFQGQARFQDGKTVLVDTDTGRQTIHAENIVIATGSVPV
HHHHHHHCCCHHHHHHHHHHHHHCCCCEECCCCEEEEECCCCCEEEEECEEEEEECCCCE
EIQALPFGGNIISSTEALSLEKIPERLAVVGGGYIGLEIGTAFAKLGSRVMVVEATDRIL
EEEEEECCCCEECCCHHHHHHHHHHHHEEECCCEEEEEHHHHHHHHCCEEEEEEECCCCC
PQYDAELTRPVMARLKTLGVEVLTGTSAKGLSADGKALEIRTQDGAVKAIEADKILVTVG
CCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEEEECCCCEEEEECCEEEEEEC
RKPQTDGWGLSEIRLDMDGRFIRIDDRCRTSMRGIYAIGDVTGEPMLAHRAMAQGEMVAE
CCCCCCCCCCEEEEECCCCCEEEECHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHH
IIAGGKHAWDKRCIPAVCFTDPEIVTVGLSLDEARKAGHNIQTGLFPFQANGRAMTMERD
HHCCCCCCCCCCCCCEEEECCCCEEEEECCHHHHHHCCCCCCCCEEEEECCCEEEEEECC
DGIVRVVARADNHVILGIQAVGAGISELSAAFAQAVEMGARLEDIAATIHAHPTLGEGFA
CCEEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHH
EASMKALGHALHV
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]