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The map label for this gene is nanEK [H]

Identifier: 225686221

GI number: 225686221

Start: 375276

End: 376853

Strand: Direct

Name: nanEK [H]

Synonym: BMEA_B0389

Alternate gene names: 225686221

Gene position: 375276-376853 (Clockwise)

Preceding gene: 225686213

Following gene: 225686222

Centisome position: 31.66

GC content: 61.66

Gene sequence:

>1578_bases
ATGCGCGGCTCGCCACAAAATTTGTGCAGGGTGCAAGGCATGATTGAAGAGATAGTCAACAGGCTGCGCGGCAAGCTTAT
CGTTTCATGCCAGCCGGTGCCGGAAAGCCCGTTTGATAATGTTGCGTCGGTTGTGGCTTATGCGCGGGCGGCGGAGGCAT
CGGGCGCAAGCGGATTGCGCATTGAGGGCGCGGCCAATGTTGCGGCCGCCGCACAGGCAAGCACCCTTCCGGTGATCGGG
CTTATAAAGCGTGATCTCGATGACAGCCCGGTCCGCATCACGCCCTTTCTGGAAGATGTAGCGGCGCTTTGCGATGCGGG
GGCGGCCATTGTGGCGGTTGATGCGACGGATCGGCGGCGTCCGGTTCCCGCAGCCGAACTGATTGGTGAAATCAAGCGGC
GTGGGCGGATTGCCATGGCCGATATTTCCACGCTGGCGGAGGCGCGCAACGCTCTTGCAGCGGGTGCGGATATTATCGGC
ACCACCATGTCAGGCTATACGGGCGAAGGCCCGACGCCCAAAGATCCTGATCTCGATCTGGTTGCGCATTGTTCTCGCCT
GGGCAGCTTTCTCATCGCGGAAGGGCGTTACAACAGCCCGCAGCAGGCGGGTGAGGCGATCCGTGCCGGAGCGGATGCCG
TGGTTGTCGGCTCTGCGATCACGAGGCCGGAACATATTACCGGCTGGTTCCGCGATGCCGTGGAAAGCGCGGCAAAGCCA
TCGTCGCCCGTGCTCGCCTTTGACATTGGCGGAACCAAGACGCTGGCTGCACTGGTCCGGGGCCGGGAAATTCTGGAGCG
CCGGGTTATGACAACGCCCGCTTCGGTCGGCAGTGAAAGCTGGATCGGAGCCATTGCCAGCCTCTCTGCGGATTGGCAGG
GCCGTTATCAGCGCGCCGCGATTGCCGTGACGGGGCGCGTGGACGGTGAAATATGGTCTTCGCTCAACCCTGAAACGCTT
GCCATCCCGCCGGATTATCCATTGGGGCGCAGGATGGGAGCAGCACTTGGCGCACCGGTCGAAGTTATCAACGATGCGCA
GGCTGCGGCATGGGGCGAATATCGCTTCGGTGCGGCACGCGGGCGCGACATGGTGTTTCTCACAATTTCCAGCGGCATTG
GCGGTGGCATCGTTCTTGGTGGGCGCCTCATTCGCGGTGCGCGCGGGATTGCCGGCAGTCTGGGGCAGGTTTTGGTTGCC
GGACCATCGGGCTTTGTAAGACTGGAAACCCTGGCTTCCGGTTTTGGCATTGCCAAAATGGCACTTGAAGCTGGCCATGC
GGGCGATGCGCGTTCGGTTTTTTCCGCAGCGGCAGCGGGCGAGGGGTGGGCACGGCGCATTCTTCTGGATGCGGCCTCGC
AATTGGCTGCCGCCGTGGCGGGCCTGCAAGCGATCGTCGATCCCGAATGTATCGTGATTGGAGGCGGTGTTGGCATGGCG
GATGGCTTTCTCGATATGTTGCGAGAAGCACTTGGCAGCCATTCCGCAGTCATGCGTCCTGATATTGTTGCCGCTGAACT
GGGGGCGGATGCCGGAATTATCGGTGTGGCCGATCTGGCCGCGACATATTTTTCCTAA

Upstream 100 bases:

>100_bases
CTTGTAGGATATCTGTCAATAGGATATTTAGACGTCCGTTATTCGACTTGTCTTGGGAGATACGCCAAATGGCAATCCGA
GTGAAGAGGCTATCGCGTAA

Downstream 100 bases:

>100_bases
AACCAATGGAGTGGAGGAAATGCATGTTCAGTCTTATTCGCGCAAAACGACTGGCGATCGGTATCGCGGCTCTTGCATGG
TCTACAGGTGCCGTGATGGC

Product: N-acetylmannosamine-6-phosphate 2-epimerase

Products: NA

Alternate protein names: Putative N-acetylmannosamine-6-phosphate 2-epimerase; ManNAc-6-P epimerase; N-acetylmannosamine kinase; ManNAc kinase; N-acetyl-D-mannosamine kinase [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MRGSPQNLCRVQGMIEEIVNRLRGKLIVSCQPVPESPFDNVASVVAYARAAEASGASGLRIEGAANVAAAAQASTLPVIG
LIKRDLDDSPVRITPFLEDVAALCDAGAAIVAVDATDRRRPVPAAELIGEIKRRGRIAMADISTLAEARNALAAGADIIG
TTMSGYTGEGPTPKDPDLDLVAHCSRLGSFLIAEGRYNSPQQAGEAIRAGADAVVVGSAITRPEHITGWFRDAVESAAKP
SSPVLAFDIGGTKTLAALVRGREILERRVMTTPASVGSESWIGAIASLSADWQGRYQRAAIAVTGRVDGEIWSSLNPETL
AIPPDYPLGRRMGAALGAPVEVINDAQAAAWGEYRFGAARGRDMVFLTISSGIGGGIVLGGRLIRGARGIAGSLGQVLVA
GPSGFVRLETLASGFGIAKMALEAGHAGDARSVFSAAAAGEGWARRILLDAASQLAAAVAGLQAIVDPECIVIGGGVGMA
DGFLDMLREALGSHSAVMRPDIVAAELGADAGIIGVADLAATYFS

Sequences:

>Translated_525_residues
MRGSPQNLCRVQGMIEEIVNRLRGKLIVSCQPVPESPFDNVASVVAYARAAEASGASGLRIEGAANVAAAAQASTLPVIG
LIKRDLDDSPVRITPFLEDVAALCDAGAAIVAVDATDRRRPVPAAELIGEIKRRGRIAMADISTLAEARNALAAGADIIG
TTMSGYTGEGPTPKDPDLDLVAHCSRLGSFLIAEGRYNSPQQAGEAIRAGADAVVVGSAITRPEHITGWFRDAVESAAKP
SSPVLAFDIGGTKTLAALVRGREILERRVMTTPASVGSESWIGAIASLSADWQGRYQRAAIAVTGRVDGEIWSSLNPETL
AIPPDYPLGRRMGAALGAPVEVINDAQAAAWGEYRFGAARGRDMVFLTISSGIGGGIVLGGRLIRGARGIAGSLGQVLVA
GPSGFVRLETLASGFGIAKMALEAGHAGDARSVFSAAAAGEGWARRILLDAASQLAAAVAGLQAIVDPECIVIGGGVGMA
DGFLDMLREALGSHSAVMRPDIVAAELGADAGIIGVADLAATYFS
>Mature_525_residues
MRGSPQNLCRVQGMIEEIVNRLRGKLIVSCQPVPESPFDNVASVVAYARAAEASGASGLRIEGAANVAAAAQASTLPVIG
LIKRDLDDSPVRITPFLEDVAALCDAGAAIVAVDATDRRRPVPAAELIGEIKRRGRIAMADISTLAEARNALAAGADIIG
TTMSGYTGEGPTPKDPDLDLVAHCSRLGSFLIAEGRYNSPQQAGEAIRAGADAVVVGSAITRPEHITGWFRDAVESAAKP
SSPVLAFDIGGTKTLAALVRGREILERRVMTTPASVGSESWIGAIASLSADWQGRYQRAAIAVTGRVDGEIWSSLNPETL
AIPPDYPLGRRMGAALGAPVEVINDAQAAAWGEYRFGAARGRDMVFLTISSGIGGGIVLGGRLIRGARGIAGSLGQVLVA
GPSGFVRLETLASGFGIAKMALEAGHAGDARSVFSAAAAGEGWARRILLDAASQLAAAVAGLQAIVDPECIVIGGGVGMA
DGFLDMLREALGSHSAVMRPDIVAAELGADAGIIGVADLAATYFS

Specific function: Catalyzes the phosphorylation of N-acetylmannosamine (ManNAc) to ManNAc-6-P [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ROK (nagC/xylR) family. NanK subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789617, Length=215, Percent_Identity=54.4186046511628, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI87082230, Length=276, Percent_Identity=38.4057971014493, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR007260
- InterPro:   IPR011060
- InterPro:   IPR000600 [H]

Pfam domain/function: PF04131 NanE; PF00480 ROK [H]

EC number: =5.1.3.9; =2.7.1.60 [H]

Molecular weight: Translated: 53859; Mature: 53859

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGSPQNLCRVQGMIEEIVNRLRGKLIVSCQPVPESPFDNVASVVAYARAAEASGASGLR
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEE
IEGAANVAAAAQASTLPVIGLIKRDLDDSPVRITPFLEDVAALCDAGAAIVAVDATDRRR
EECCCCHHHHHHCCCCCHHHHHHCCCCCCCEEEEHHHHHHHHHHCCCCEEEEEECCCCCC
PVPAAELIGEIKRRGRIAMADISTLAEARNALAAGADIIGTTMSGYTGEGPTPKDPDLDL
CCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHH
VAHCSRLGSFLIAEGRYNSPQQAGEAIRAGADAVVVGSAITRPEHITGWFRDAVESAAKP
HHHHHHHCCEEEECCCCCCHHHHHHHHHCCCCEEEECCHHCCCHHHHHHHHHHHHHHCCC
SSPVLAFDIGGTKTLAALVRGREILERRVMTTPASVGSESWIGAIASLSADWQGRYQRAA
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEE
IAVTGRVDGEIWSSLNPETLAIPPDYPLGRRMGAALGAPVEVINDAQAAAWGEYRFGAAR
EEEEECCCHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCHHHHCCCHHHHCCCCCCCCCC
GRDMVFLTISSGIGGGIVLGGRLIRGARGIAGSLGQVLVAGPSGFVRLETLASGFGIAKM
CCCEEEEEECCCCCCCEEECCHHHHCCCCCCCCCCCEEEECCCCCEEHHHHHCCCCHHHH
ALEAGHAGDARSVFSAAAAGEGWARRILLDAASQLAAAVAGLQAIVDPECIVIGGGVGMA
HHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHH
DGFLDMLREALGSHSAVMRPDIVAAELGADAGIIGVADLAATYFS
HHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEHHHHHHHHCC
>Mature Secondary Structure
MRGSPQNLCRVQGMIEEIVNRLRGKLIVSCQPVPESPFDNVASVVAYARAAEASGASGLR
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEE
IEGAANVAAAAQASTLPVIGLIKRDLDDSPVRITPFLEDVAALCDAGAAIVAVDATDRRR
EECCCCHHHHHHCCCCCHHHHHHCCCCCCCEEEEHHHHHHHHHHCCCCEEEEEECCCCCC
PVPAAELIGEIKRRGRIAMADISTLAEARNALAAGADIIGTTMSGYTGEGPTPKDPDLDL
CCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHH
VAHCSRLGSFLIAEGRYNSPQQAGEAIRAGADAVVVGSAITRPEHITGWFRDAVESAAKP
HHHHHHHCCEEEECCCCCCHHHHHHHHHCCCCEEEECCHHCCCHHHHHHHHHHHHHHCCC
SSPVLAFDIGGTKTLAALVRGREILERRVMTTPASVGSESWIGAIASLSADWQGRYQRAA
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEE
IAVTGRVDGEIWSSLNPETLAIPPDYPLGRRMGAALGAPVEVINDAQAAAWGEYRFGAAR
EEEEECCCHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCHHHHCCCHHHHCCCCCCCCCC
GRDMVFLTISSGIGGGIVLGGRLIRGARGIAGSLGQVLVAGPSGFVRLETLASGFGIAKM
CCCEEEEEECCCCCCCEEECCHHHHCCCCCCCCCCCEEEECCCCCEEHHHHHCCCCHHHH
ALEAGHAGDARSVFSAAAAGEGWARRILLDAASQLAAAVAGLQAIVDPECIVIGGGVGMA
HHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHH
DGFLDMLREALGSHSAVMRPDIVAAELGADAGIIGVADLAATYFS
HHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]