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The map label for this gene is infB [H]

Identifier: 225631018

GI number: 225631018

Start: 1436983

End: 1439307

Strand: Reverse

Name: infB [H]

Synonym: WRi_013480

Alternate gene names: 225631018

Gene position: 1439307-1436983 (Counterclockwise)

Preceding gene: 225631019

Following gene: 225631017

Centisome position: 99.55

GC content: 34.67

Gene sequence:

>2325_bases
ATGAATAATGAAGATATCAGTGATAAAAAATTAACGCTCCAAGGTTTAAGCAAGCTTAAATTAGACCTCAATTTAAACTC
TTCAGCTAGCCCAAGTACTGGAGCTACAGTAGTAAAAAAAAGAAGAAAAAAATCTCATGATGCAGAAGAACATAATTTAT
TTGACGAGAGTAAATTAGGTTCTTTAACAGAGAAAGAACAAATTTTTCGCATTAATGCTGTGCAGAATGCTGCTTTGCTG
AAAGAAAGAAATCAGAGAGAAGAAAAAGAGAAAACAGCAAAAGAAGACAGCAATAAAGAGACTGACGAGAAAAATGAAGC
GGACGCTTTATCTAAGGAAATAAATAAGCAAGTTTTAAGTAATACCCACTTAGTTGAACCAAAAGAAGATAGTATTGACC
ATGAGGACAACGATAAAAAGTCTTTTAAAGTCAACAAAGATATATATTCTAAGCATTCTAAACTGGTAATTGCACAGGCA
ATAGATGAAAAAACTGAACAACCCCCTGTATTTAAGCAAAGGTTTGGTATAAGAGGTAGAAAGTCAAAGTTTACTAAGGG
TAAAAATATATCAAGAGAAGTTATTATACCAGACAAAATAACTATCAGGGAGTTATCTATTCGCATGGCAGAAGATAGCA
AGAGTGTGCTAAAAATGTTCAAAGAAGAAGTCGGTGAGAGTTATAGAGTAGATGATCTGGTGGATCCAGATATAGCATGT
GAAATAGTGGAAAAATTTAATCATACGGCTAAACGAGTGAGCGATGCCAACAAAGAAAAGGATTTATTTTTTATAGAAGG
CAGAGAAAGCCTGCCTAAAAAACCTAAACCACCAATCGTCACTTTTATGGGACACGTCGATCATGGTAAAACCTCATTGC
TCGATGCATTTCGTGAATCTAATGTTGCAGAAAGAGAGTCAGGTGGCATAACTCAACACATAGGTGCTTATCAAATAATA
ACAAAAGATAAGCAAAAAATTACTTTCATTGATACACCAGGACATGAAGCGTTCACTGCGATGCGTGCATGTGGTGCCAA
TATTACTAATATAGTTGTAATAGTAGTTGCAGCTGACGATGGAATCATGAAACAAACAGTTGAAGCAATAAATCATGCAA
AAGCAGCAAATGTTTCCATTATAGTTGCTATTAATAAAATTGATAAATCTGAGCCTGGTGATGTAGAAAAAATAATTAAT
AGTTTGCCTCAATATGATCTCATCCCTGAAGGGCTTGGTGGTGATGTTATGATTGTGCCAGTATCAGCAAAAAAGAAAAC
TAATTTAGATAAACTAGAAGAGACGATTTTGTTAATCGCTGAATTAATGAAGCTAGAAGCAATAGAAGATTGTCGAGCAC
TTGGATGGGTAATAGAATCCAAAATAGATAAAGCGAAAGGAATATCAGCCACATTGATAGTTGAGGAAGGAACGTTAAAG
GTTGGTGATATATTGGTAGTTGGTACAACATACAGCAAAGTACGTAGTATGGTTAATCACCTCGGTCAAAGAGAAAAAGC
AGCACTACCCTCCGCTCCAATTGAAATTACTGGTCTAAATGGTGTGCCAAATGCTGGAGATAAATTTGTTGTTATAAATT
CTGAAAAGCAGGCGCGTGAAATTGTTGAATACAGGCTAGAGTTAATCAAGAAAAAGAAAGAAGATTTGGGCGACAATAAT
TTAGATATATTCAGTCGTAATAACAGCGAAACAGAAGAACTATCTGTAGTTTTAAAGTGCGATGTAACTGGTTCTATTGA
AGCAATATCAAGTTCAATTGATAAACTCGGTAAAGACCAAGTGAAATTAAATATCCTACACAAAGCAGTAGGAGGGATAA
CAGATTCAGATGTGCTGCTTGCAGAAGCATCAAGTGCAGTAATTTTAGCTTTTAATGTAAAAGTGGACTCAAAAATAAGG
GACTTGGCAAAACAAAAAGGTATAGAAATACATACTTATAACATAATATACGAGCTCATTGACGACATGAGAATGTATTT
AACTAAAATGTTAAAGCCTGTTACACGAGAGGTTCGTGTTGGCTTTGCATCTGTGAGGCAAATATTTAACGTATCTAAAG
CTGGTAATATCATTGGATGTTATGTAAGTGATGGAGTCATAAGAAAAGATTCTTTAATTAAGGTAATGCGTGGTGGCAAA
TTGGTGCATGAAGGAAAGTTAAAAGCACTACGTAGATTTAAAGATGATGTTAAAGAAGTAGGTGTAAACTTTGAATGCGG
AGTATCACTAGAGGGTAATATCGATATTAAAGTTGGAGATATTTTAGAAGCTTATCAATTAGTGCAAGAAGAAAGGGTAT
TATAG

Upstream 100 bases:

>100_bases
ACTCTCTTCCTCATCCGATAGCAAAGAAGATCTAAAAGATATAGTAGATTCAATAATCATAGAAGCACGTAAAAAGCTTG
GATTAATATAATAAAATAAT

Downstream 100 bases:

>100_bases
TAGCTGGATATGAAAAAGGAAATTAGAAACCTAAAAATAGCATCGGTATTGCATAGAGCGATATCAAGAGTCCTAATGGA
AGGTAAGGTGTTCAATAAAA

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 774; Mature: 774

Protein sequence:

>774_residues
MNNEDISDKKLTLQGLSKLKLDLNLNSSASPSTGATVVKKRRKKSHDAEEHNLFDESKLGSLTEKEQIFRINAVQNAALL
KERNQREEKEKTAKEDSNKETDEKNEADALSKEINKQVLSNTHLVEPKEDSIDHEDNDKKSFKVNKDIYSKHSKLVIAQA
IDEKTEQPPVFKQRFGIRGRKSKFTKGKNISREVIIPDKITIRELSIRMAEDSKSVLKMFKEEVGESYRVDDLVDPDIAC
EIVEKFNHTAKRVSDANKEKDLFFIEGRESLPKKPKPPIVTFMGHVDHGKTSLLDAFRESNVAERESGGITQHIGAYQII
TKDKQKITFIDTPGHEAFTAMRACGANITNIVVIVVAADDGIMKQTVEAINHAKAANVSIIVAINKIDKSEPGDVEKIIN
SLPQYDLIPEGLGGDVMIVPVSAKKKTNLDKLEETILLIAELMKLEAIEDCRALGWVIESKIDKAKGISATLIVEEGTLK
VGDILVVGTTYSKVRSMVNHLGQREKAALPSAPIEITGLNGVPNAGDKFVVINSEKQAREIVEYRLELIKKKKEDLGDNN
LDIFSRNNSETEELSVVLKCDVTGSIEAISSSIDKLGKDQVKLNILHKAVGGITDSDVLLAEASSAVILAFNVKVDSKIR
DLAKQKGIEIHTYNIIYELIDDMRMYLTKMLKPVTREVRVGFASVRQIFNVSKAGNIIGCYVSDGVIRKDSLIKVMRGGK
LVHEGKLKALRRFKDDVKEVGVNFECGVSLEGNIDIKVGDILEAYQLVQEERVL

Sequences:

>Translated_774_residues
MNNEDISDKKLTLQGLSKLKLDLNLNSSASPSTGATVVKKRRKKSHDAEEHNLFDESKLGSLTEKEQIFRINAVQNAALL
KERNQREEKEKTAKEDSNKETDEKNEADALSKEINKQVLSNTHLVEPKEDSIDHEDNDKKSFKVNKDIYSKHSKLVIAQA
IDEKTEQPPVFKQRFGIRGRKSKFTKGKNISREVIIPDKITIRELSIRMAEDSKSVLKMFKEEVGESYRVDDLVDPDIAC
EIVEKFNHTAKRVSDANKEKDLFFIEGRESLPKKPKPPIVTFMGHVDHGKTSLLDAFRESNVAERESGGITQHIGAYQII
TKDKQKITFIDTPGHEAFTAMRACGANITNIVVIVVAADDGIMKQTVEAINHAKAANVSIIVAINKIDKSEPGDVEKIIN
SLPQYDLIPEGLGGDVMIVPVSAKKKTNLDKLEETILLIAELMKLEAIEDCRALGWVIESKIDKAKGISATLIVEEGTLK
VGDILVVGTTYSKVRSMVNHLGQREKAALPSAPIEITGLNGVPNAGDKFVVINSEKQAREIVEYRLELIKKKKEDLGDNN
LDIFSRNNSETEELSVVLKCDVTGSIEAISSSIDKLGKDQVKLNILHKAVGGITDSDVLLAEASSAVILAFNVKVDSKIR
DLAKQKGIEIHTYNIIYELIDDMRMYLTKMLKPVTREVRVGFASVRQIFNVSKAGNIIGCYVSDGVIRKDSLIKVMRGGK
LVHEGKLKALRRFKDDVKEVGVNFECGVSLEGNIDIKVGDILEAYQLVQEERVL
>Mature_774_residues
MNNEDISDKKLTLQGLSKLKLDLNLNSSASPSTGATVVKKRRKKSHDAEEHNLFDESKLGSLTEKEQIFRINAVQNAALL
KERNQREEKEKTAKEDSNKETDEKNEADALSKEINKQVLSNTHLVEPKEDSIDHEDNDKKSFKVNKDIYSKHSKLVIAQA
IDEKTEQPPVFKQRFGIRGRKSKFTKGKNISREVIIPDKITIRELSIRMAEDSKSVLKMFKEEVGESYRVDDLVDPDIAC
EIVEKFNHTAKRVSDANKEKDLFFIEGRESLPKKPKPPIVTFMGHVDHGKTSLLDAFRESNVAERESGGITQHIGAYQII
TKDKQKITFIDTPGHEAFTAMRACGANITNIVVIVVAADDGIMKQTVEAINHAKAANVSIIVAINKIDKSEPGDVEKIIN
SLPQYDLIPEGLGGDVMIVPVSAKKKTNLDKLEETILLIAELMKLEAIEDCRALGWVIESKIDKAKGISATLIVEEGTLK
VGDILVVGTTYSKVRSMVNHLGQREKAALPSAPIEITGLNGVPNAGDKFVVINSEKQAREIVEYRLELIKKKKEDLGDNN
LDIFSRNNSETEELSVVLKCDVTGSIEAISSSIDKLGKDQVKLNILHKAVGGITDSDVLLAEASSAVILAFNVKVDSKIR
DLAKQKGIEIHTYNIIYELIDDMRMYLTKMLKPVTREVRVGFASVRQIFNVSKAGNIIGCYVSDGVIRKDSLIKVMRGGK
LVHEGKLKALRRFKDDVKEVGVNFECGVSLEGNIDIKVGDILEAYQLVQEERVL

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=537, Percent_Identity=40.5959031657356, Blast_Score=378, Evalue=1e-104,
Organism=Homo sapiens, GI53729337, Length=537, Percent_Identity=40.5959031657356, Blast_Score=378, Evalue=1e-104,
Organism=Homo sapiens, GI84043963, Length=466, Percent_Identity=26.8240343347639, Blast_Score=125, Evalue=2e-28,
Organism=Escherichia coli, GI1789559, Length=593, Percent_Identity=43.8448566610455, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI2367247, Length=226, Percent_Identity=27.8761061946903, Blast_Score=73, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71994658, Length=543, Percent_Identity=35.3591160220994, Blast_Score=309, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI212656558, Length=537, Percent_Identity=23.2774674115456, Blast_Score=103, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324550, Length=651, Percent_Identity=35.3302611367127, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319282, Length=506, Percent_Identity=27.6679841897233, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI28572034, Length=576, Percent_Identity=38.1944444444444, Blast_Score=359, Evalue=4e-99,
Organism=Drosophila melanogaster, GI160714833, Length=120, Percent_Identity=45.8333333333333, Blast_Score=109, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24656849, Length=128, Percent_Identity=38.28125, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 86038; Mature: 86038

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNEDISDKKLTLQGLSKLKLDLNLNSSASPSTGATVVKKRRKKSHDAEEHNLFDESKLG
CCCCCCCCCCEEHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCHHHCC
SLTEKEQIFRINAVQNAALLKERNQREEKEKTAKEDSNKETDEKNEADALSKEINKQVLS
CCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
NTHLVEPKEDSIDHEDNDKKSFKVNKDIYSKHSKLVIAQAIDEKTEQPPVFKQRFGIRGR
CCCCCCCCCCCCCCCCCCCCCEEECHHHHHCCCCEEEEHHHHHCCCCCCHHHHHCCCCCC
KSKFTKGKNISREVIIPDKITIRELSIRMAEDSKSVLKMFKEEVGESYRVDDLVDPDIAC
CCCCCCCCCCCCEEECCCCEEEEEHEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
EIVEKFNHTAKRVSDANKEKDLFFIEGRESLPKKPKPPIVTFMGHVDHGKTSLLDAFRES
HHHHHHHHHHHHHHCCCCCCCEEEEECCHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHC
NVAERESGGITQHIGAYQIITKDKQKITFIDTPGHEAFTAMRACGANITNIVVIVVAADD
CCCHHHCCCCHHHCCCEEEEECCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEECCC
GIMKQTVEAINHAKAANVSIIVAINKIDKSEPGDVEKIINSLPQYDLIPEGLGGDVMIVP
CHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEE
VSAKKKTNLDKLEETILLIAELMKLEAIEDCRALGWVIESKIDKAKGISATLIVEEGTLK
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEE
VGDILVVGTTYSKVRSMVNHLGQREKAALPSAPIEITGLNGVPNAGDKFVVINSEKQARE
ECCEEEECCCHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCEEEEECCCHHHHH
IVEYRLELIKKKKEDLGDNNLDIFSRNNSETEELSVVLKCDVTGSIEAISSSIDKLGKDQ
HHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHH
VKLNILHKAVGGITDSDVLLAEASSAVILAFNVKVDSKIRDLAKQKGIEIHTYNIIYELI
HHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEEEECHHHHHHHHHCCCEEEHHHHHHHHH
DDMRMYLTKMLKPVTREVRVGFASVRQIFNVSKAGNIIGCYVSDGVIRKDSLIKVMRGGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECHHHHHHHHHCCC
LVHEGKLKALRRFKDDVKEVGVNFECGVSLEGNIDIKVGDILEAYQLVQEERVL
EEEHHHHHHHHHHHHHHHHHCCCEEECEEECCCEEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNEDISDKKLTLQGLSKLKLDLNLNSSASPSTGATVVKKRRKKSHDAEEHNLFDESKLG
CCCCCCCCCCEEHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCHHHCC
SLTEKEQIFRINAVQNAALLKERNQREEKEKTAKEDSNKETDEKNEADALSKEINKQVLS
CCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
NTHLVEPKEDSIDHEDNDKKSFKVNKDIYSKHSKLVIAQAIDEKTEQPPVFKQRFGIRGR
CCCCCCCCCCCCCCCCCCCCCEEECHHHHHCCCCEEEEHHHHHCCCCCCHHHHHCCCCCC
KSKFTKGKNISREVIIPDKITIRELSIRMAEDSKSVLKMFKEEVGESYRVDDLVDPDIAC
CCCCCCCCCCCCEEECCCCEEEEEHEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
EIVEKFNHTAKRVSDANKEKDLFFIEGRESLPKKPKPPIVTFMGHVDHGKTSLLDAFRES
HHHHHHHHHHHHHHCCCCCCCEEEEECCHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHC
NVAERESGGITQHIGAYQIITKDKQKITFIDTPGHEAFTAMRACGANITNIVVIVVAADD
CCCHHHCCCCHHHCCCEEEEECCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEECCC
GIMKQTVEAINHAKAANVSIIVAINKIDKSEPGDVEKIINSLPQYDLIPEGLGGDVMIVP
CHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEE
VSAKKKTNLDKLEETILLIAELMKLEAIEDCRALGWVIESKIDKAKGISATLIVEEGTLK
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEE
VGDILVVGTTYSKVRSMVNHLGQREKAALPSAPIEITGLNGVPNAGDKFVVINSEKQARE
ECCEEEECCCHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCEEEEECCCHHHHH
IVEYRLELIKKKKEDLGDNNLDIFSRNNSETEELSVVLKCDVTGSIEAISSSIDKLGKDQ
HHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHH
VKLNILHKAVGGITDSDVLLAEASSAVILAFNVKVDSKIRDLAKQKGIEIHTYNIIYELI
HHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEEEECHHHHHHHHHCCCEEEHHHHHHHHH
DDMRMYLTKMLKPVTREVRVGFASVRQIFNVSKAGNIIGCYVSDGVIRKDSLIKVMRGGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECHHHHHHHHHCCC
LVHEGKLKALRRFKDDVKEVGVNFECGVSLEGNIDIKVGDILEAYQLVQEERVL
EEEHHHHHHHHHHHHHHHHHCCCEEECEEECCCEEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA