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The map label for this gene is rpoD [H]

Identifier: 225630998

GI number: 225630998

Start: 1413981

End: 1415933

Strand: Reverse

Name: rpoD [H]

Synonym: WRi_013260

Alternate gene names: 225630998

Gene position: 1415933-1413981 (Counterclockwise)

Preceding gene: 225630999

Following gene: 225630997

Centisome position: 97.93

GC content: 34.15

Gene sequence:

>1953_bases
ATGTCAACTATTAATGATGCAGAAGATAAGAAAAAAATTATCAAAGATCTGGTAACTAAGAGTATAAAAAAGGGTGGTTT
TATCACCTTTGATGATATAAATGATAAATTATCAGATGAAAATTTTTCGTCTGACTTTATAGATGATGCTATATCTTTAT
TGCAGGATTCCGGAATTAATGTGCTTGAAAGTAGCGAAGATGAAGAAGATATTCCTTCCAACAGTGATGATAGTAAACTT
GATGATGATGACACATCGCCAAATGTTACTGCAACTTTAGTACAAAATGATGATCCAGTGAGGATTTATTTACAAGACAT
GAGCTCCGTAAAGCTTTTATCAAGGGCAGATGAAATCGAGATAGCAAAAAAAATTGAATCTGAAAAGCATAACATGTTAC
GTGCAATAATTGAAACATCAGTAACATTAAAGGTGATAAAAGTATGGCGTGATGATTTAAGTAATGGAGCTCTCTTACTG
AGAGAAATTATAGATCTGGATGCAATTTATAACTCTGATTTTAATGTGGTACCCAAAGAAGAAAGTGAAGAGAGTGAAGA
AAGTGCTGAAGATGTTGAGGATTCTGAGGATGTTTCTGAAGATGCTGAGTACTGTAGTGATGATGAGAAAGATAGTAAAA
AAAGTGATGATGAGAAAGACGGTAATAATGAAGATATAAGTTCAGTCAATTTAAATGTTTCTATTCTTGAAATGGAAAGT
GACTTATTGCCAAAGGTCATAGTTGCATTGGATGAGATAATTACTTTAACAAATGAAGCGTTGATATTAAGAAAAAATTC
TAAAAATTTCTCTAATGAGCTAGAAAATTTGTATAATCAAATATGGTCTATAGCATTACAAATTAAGTTAAGTGATGCTG
CTGTTGCCAGAATTACACAAGAACTTTATAAGATAAATAGATCCATTACGCTTGAAGAGGCTAATCTTATTGCTGAGGCT
AGAAAATACGATATCGATAGAGAAAGTTTCTATAAAGTTTATGATGACGTGCTTTTAAAGCACGAATTGAAAAAGATAAG
TCTTTTAAAGATCAATGAAAATCAGTCCTCTTTTACAGGTGAATTAAAAAACAAATTTTTAAGGTTTATAGACGATAACT
CTGAACATATAGCTAGTACCTTGAACGGTATTAAGCAGCATATACAGGAAGATAACGTACAAGAATTTAAGGAGCTAATC
AAGAGAATACAAAAACACGAACGAGAAGTCTCTGAAGCAAAGCAAGAAATGATTAAGGCTAACTTAAGGCTAGTAGTTTC
CATTGCTAAGAAGTATTCAAAAAGAGGCCTTGATCTGCTTGATTTGATACAAGAAGGCAATATTGGTCTTATGAAGGCCG
TGGATAAGTTTGATTACAAACGTGGATATAAATTTTCAACTTATGGCACTTGGTGGGTAAGACAATCAATCACTAGAGCA
ATACCTGAGCAGTCTAAAGTAGTTAGAATACCAGTTCATATGGTGGAAATTATCAGTAAAATCAACAGAGCATTAAGAAA
GATGACTCATGAGATGGGAAGAGAGCCGACGTTAGAGGAATTGAGTGTAGAACTGACAATGCCACTAGAAAGAATACGCA
AAGTTATGAAAATAGCAAGGGATCCAGTAAGTCTTGAAGCTCCAACAGGAAAAGATGATAGTAGTACCTTTGGTGATTGT
ATAGAAGACAAGCGAGTCTCCAGGCCAGAGGATGCTGCAATACTTGCTGACTTGCGTGGCATTACAACCAATGTTCTTGC
AACTTTAACACCAAAGGAAGAAAGAATTTTAAGAATGCGTTTTGGCCTTGGTAAAGATGGGAAGGAACATACCTTAGAAG
AAGTAGGAAAAATTTTTAATGTAACACGTGAGAGAATTAGGCAAATAGAGGCAAAAGCACTGCGCAAGTTGAAACATCCA
AGCCGTGCTAGAAAACTTAGAGGGTTCTTTTAA

Upstream 100 bases:

>100_bases
GAAGTAACAAATAGCCACAAGTTAAAAGTAAAATTATAGACTTGCTCGTTGTAAGTTTGTTTGTTAATAAGAAATAAAAT
TATAAACTATAAAGGTAATG

Downstream 100 bases:

>100_bases
GTATACGTGTCAAGTTAAGAAGAAGCTGATTTTGCCCGGAAAAAGTGGAGAGCAAGTTAGGCCAAAGTTGTATAATTTCA
CCGCTATTTTTCTTTTTCTA

Product: RNA polymerase sigma 70 subunit, RpoD

Products: NA

Alternate protein names: Sigma-A; Major vegetative sigma factor [H]

Number of amino acids: Translated: 650; Mature: 649

Protein sequence:

>650_residues
MSTINDAEDKKKIIKDLVTKSIKKGGFITFDDINDKLSDENFSSDFIDDAISLLQDSGINVLESSEDEEDIPSNSDDSKL
DDDDTSPNVTATLVQNDDPVRIYLQDMSSVKLLSRADEIEIAKKIESEKHNMLRAIIETSVTLKVIKVWRDDLSNGALLL
REIIDLDAIYNSDFNVVPKEESEESEESAEDVEDSEDVSEDAEYCSDDEKDSKKSDDEKDGNNEDISSVNLNVSILEMES
DLLPKVIVALDEIITLTNEALILRKNSKNFSNELENLYNQIWSIALQIKLSDAAVARITQELYKINRSITLEEANLIAEA
RKYDIDRESFYKVYDDVLLKHELKKISLLKINENQSSFTGELKNKFLRFIDDNSEHIASTLNGIKQHIQEDNVQEFKELI
KRIQKHEREVSEAKQEMIKANLRLVVSIAKKYSKRGLDLLDLIQEGNIGLMKAVDKFDYKRGYKFSTYGTWWVRQSITRA
IPEQSKVVRIPVHMVEIISKINRALRKMTHEMGREPTLEELSVELTMPLERIRKVMKIARDPVSLEAPTGKDDSSTFGDC
IEDKRVSRPEDAAILADLRGITTNVLATLTPKEERILRMRFGLGKDGKEHTLEEVGKIFNVTRERIRQIEAKALRKLKHP
SRARKLRGFF

Sequences:

>Translated_650_residues
MSTINDAEDKKKIIKDLVTKSIKKGGFITFDDINDKLSDENFSSDFIDDAISLLQDSGINVLESSEDEEDIPSNSDDSKL
DDDDTSPNVTATLVQNDDPVRIYLQDMSSVKLLSRADEIEIAKKIESEKHNMLRAIIETSVTLKVIKVWRDDLSNGALLL
REIIDLDAIYNSDFNVVPKEESEESEESAEDVEDSEDVSEDAEYCSDDEKDSKKSDDEKDGNNEDISSVNLNVSILEMES
DLLPKVIVALDEIITLTNEALILRKNSKNFSNELENLYNQIWSIALQIKLSDAAVARITQELYKINRSITLEEANLIAEA
RKYDIDRESFYKVYDDVLLKHELKKISLLKINENQSSFTGELKNKFLRFIDDNSEHIASTLNGIKQHIQEDNVQEFKELI
KRIQKHEREVSEAKQEMIKANLRLVVSIAKKYSKRGLDLLDLIQEGNIGLMKAVDKFDYKRGYKFSTYGTWWVRQSITRA
IPEQSKVVRIPVHMVEIISKINRALRKMTHEMGREPTLEELSVELTMPLERIRKVMKIARDPVSLEAPTGKDDSSTFGDC
IEDKRVSRPEDAAILADLRGITTNVLATLTPKEERILRMRFGLGKDGKEHTLEEVGKIFNVTRERIRQIEAKALRKLKHP
SRARKLRGFF
>Mature_649_residues
STINDAEDKKKIIKDLVTKSIKKGGFITFDDINDKLSDENFSSDFIDDAISLLQDSGINVLESSEDEEDIPSNSDDSKLD
DDDTSPNVTATLVQNDDPVRIYLQDMSSVKLLSRADEIEIAKKIESEKHNMLRAIIETSVTLKVIKVWRDDLSNGALLLR
EIIDLDAIYNSDFNVVPKEESEESEESAEDVEDSEDVSEDAEYCSDDEKDSKKSDDEKDGNNEDISSVNLNVSILEMESD
LLPKVIVALDEIITLTNEALILRKNSKNFSNELENLYNQIWSIALQIKLSDAAVARITQELYKINRSITLEEANLIAEAR
KYDIDRESFYKVYDDVLLKHELKKISLLKINENQSSFTGELKNKFLRFIDDNSEHIASTLNGIKQHIQEDNVQEFKELIK
RIQKHEREVSEAKQEMIKANLRLVVSIAKKYSKRGLDLLDLIQEGNIGLMKAVDKFDYKRGYKFSTYGTWWVRQSITRAI
PEQSKVVRIPVHMVEIISKINRALRKMTHEMGREPTLEELSVELTMPLERIRKVMKIARDPVSLEAPTGKDDSSTFGDCI
EDKRVSRPEDAAILADLRGITTNVLATLTPKEERILRMRFGLGKDGKEHTLEEVGKIFNVTRERIRQIEAKALRKLKHPS
RARKLRGFF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=284, Percent_Identity=56.3380281690141, Blast_Score=329, Evalue=3e-91,
Organism=Escherichia coli, GI1789098, Length=227, Percent_Identity=40.5286343612335, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1789871, Length=244, Percent_Identity=30.327868852459, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 74306; Mature: 74175

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTINDAEDKKKIIKDLVTKSIKKGGFITFDDINDKLSDENFSSDFIDDAISLLQDSGIN
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
VLESSEDEEDIPSNSDDSKLDDDDTSPNVTATLVQNDDPVRIYLQDMSSVKLLSRADEIE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHH
IAKKIESEKHNMLRAIIETSVTLKVIKVWRDDLSNGALLLREIIDLDAIYNSDFNVVPKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCC
ESEESEESAEDVEDSEDVSEDAEYCSDDEKDSKKSDDEKDGNNEDISSVNLNVSILEMES
CCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCH
DLLPKVIVALDEIITLTNEALILRKNSKNFSNELENLYNQIWSIALQIKLSDAAVARITQ
HHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEEEECHHHHHHHHH
ELYKINRSITLEEANLIAEARKYDIDRESFYKVYDDVLLKHELKKISLLKINENQSSFTG
HHHHHCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHH
ELKNKFLRFIDDNSEHIASTLNGIKQHIQEDNVQEFKELIKRIQKHEREVSEAKQEMIKA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLRLVVSIAKKYSKRGLDLLDLIQEGNIGLMKAVDKFDYKRGYKFSTYGTWWVRQSITRA
HHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
IPEQSKVVRIPVHMVEIISKINRALRKMTHEMGREPTLEELSVELTMPLERIRKVMKIAR
CCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHC
DPVSLEAPTGKDDSSTFGDCIEDKRVSRPEDAAILADLRGITTNVLATLTPKEERILRMR
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHH
FGLGKDGKEHTLEEVGKIFNVTRERIRQIEAKALRKLKHPSRARKLRGFF
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCC
>Mature Secondary Structure 
STINDAEDKKKIIKDLVTKSIKKGGFITFDDINDKLSDENFSSDFIDDAISLLQDSGIN
CCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
VLESSEDEEDIPSNSDDSKLDDDDTSPNVTATLVQNDDPVRIYLQDMSSVKLLSRADEIE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHH
IAKKIESEKHNMLRAIIETSVTLKVIKVWRDDLSNGALLLREIIDLDAIYNSDFNVVPKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCC
ESEESEESAEDVEDSEDVSEDAEYCSDDEKDSKKSDDEKDGNNEDISSVNLNVSILEMES
CCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCH
DLLPKVIVALDEIITLTNEALILRKNSKNFSNELENLYNQIWSIALQIKLSDAAVARITQ
HHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEEEECHHHHHHHHH
ELYKINRSITLEEANLIAEARKYDIDRESFYKVYDDVLLKHELKKISLLKINENQSSFTG
HHHHHCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHH
ELKNKFLRFIDDNSEHIASTLNGIKQHIQEDNVQEFKELIKRIQKHEREVSEAKQEMIKA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLRLVVSIAKKYSKRGLDLLDLIQEGNIGLMKAVDKFDYKRGYKFSTYGTWWVRQSITRA
HHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
IPEQSKVVRIPVHMVEIISKINRALRKMTHEMGREPTLEELSVELTMPLERIRKVMKIAR
CCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHC
DPVSLEAPTGKDDSSTFGDCIEDKRVSRPEDAAILADLRGITTNVLATLTPKEERILRMR
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHH
FGLGKDGKEHTLEEVGKIFNVTRERIRQIEAKALRKLKHPSRARKLRGFF
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491721; 11743193; 11743194 [H]