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The map label for this gene is tyrS

Identifier: 225630967

GI number: 225630967

Start: 1377965

End: 1379215

Strand: Reverse

Name: tyrS

Synonym: WRi_012890

Alternate gene names: 225630967

Gene position: 1379215-1377965 (Counterclockwise)

Preceding gene: 225630968

Following gene: 225630966

Centisome position: 95.39

GC content: 35.65

Gene sequence:

>1251_bases
ATGAAACACGCATCTGAATTTTTAAATTTCATCCAAGAAAGAGGGTACCTATACCAATGCACAAACATTGAAGGACTAGA
TCAATTATTATTGCAGAGCAATTATATAGTTGCATACATTGGGTTTGATTGCACAGCACCAAGTCTTCATGCTGGTCACC
TAATTCAGATTATGATGCTCTGTCACCTACAAAAATTTGGTTATAAGCCGATAGTCTTACTTGGAGGTGGCACAACAAAA
ATTGGTGACCCATCCTTCAAAGATAAAGCAAGAAGCATCTTGCCTGTAGAGAACATCAATCAAAATACATCCAGTATAAG
GAGAATATTAGAGAAAATGGTGTCTTTTAATGACAGCAAGACTGGTGCAATGATAGTAAATAACGCAGATTGGTTGGATA
ACATAAAATACATAGATTTTTTGCGTGATATAGGAGCTTATTTTTCTGTAAATCGTATGCTAAGTTTTGATAGTGTGAAA
ACCAGGCTGGATAGAGAGCAAACCTTAAGCTTTCTGGAATTTAATTACATGCTATTGCAAGCTTACGACTTTATTGAATT
AAACAAAAAATATGATTGTCGTCTGCAGATTGGAGGGTCAGACCAGTGGGGAAATATAGTAAACGGAATTGAACTTGGAA
AAAAGTTGAATTTACCTGAGCTATTTGGCCTTACCATGCCTCTTTTATTAAATGCACAAGGAAAAAAAATGGGCAAAACT
GAAAGTGGAGCAGTGTGGCTTGATGATAATATGCTAAAGCCTTACGACTACTGGCAATATTTTCGCAACGTTGATGATCA
AGATGTTGGACGTTTTTTAAGATTGCTCACTGATGTGCCAATTGATGAGATTAGAAAATTAGAGTCTTTAAAGGATCAAG
AAATAAATGAAGCAAAAAAGGTCTTGGCAACAGAAGTGACAAAAATATGTCATGGTGACAAAGAAGCGGAACTTGCACAA
TTTGCTGCAATTTCTGCTTTTGAGAATGAAGATAGCTCACTACTTCCTGATTACATCATAAAAAAAGAACAAGTTGCAAG
CGGCATATCCTTAGTAGACCTACTACATGACACCGGTTTTGAACCTTCAAAAGGCGCTGCAAAGCGTTTAATACAGGGCA
ACGGATGCAAGATTAATGATAATGTTGTAAACAATATTGATCATGTAATAAATTTCGAAAGCTTTAAAGATCAGCCATTT
ATAAAATTATCTGCAGGAAAGAAACGCCATATAAAAGTTGTGGTAGATTAG

Upstream 100 bases:

>100_bases
ATAGTGTGCTTGTTTATAATTAAGTTCCAGTGTCTGGGCACTGGCTTCTCTATTAGGGTTACCTTCGGCATTAATGACAG
GATAGACTGGGGTTTATAAA

Downstream 100 bases:

>100_bases
GTCTCGAAGTAAGAAAACAAATTTTATACAAGTGAGAAATTAGATATGGAGCAAATTTTCAAAATTCGAGAATTATGTAT
AAAACAACTGGAAAGATAGT

Product: Tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKHASEFLNFIQERGYLYQCTNIEGLDQLLLQSNYIVAYIGFDCTAPSLHAGHLIQIMMLCHLQKFGYKPIVLLGGGTTK
IGDPSFKDKARSILPVENINQNTSSIRRILEKMVSFNDSKTGAMIVNNADWLDNIKYIDFLRDIGAYFSVNRMLSFDSVK
TRLDREQTLSFLEFNYMLLQAYDFIELNKKYDCRLQIGGSDQWGNIVNGIELGKKLNLPELFGLTMPLLLNAQGKKMGKT
ESGAVWLDDNMLKPYDYWQYFRNVDDQDVGRFLRLLTDVPIDEIRKLESLKDQEINEAKKVLATEVTKICHGDKEAELAQ
FAAISAFENEDSSLLPDYIIKKEQVASGISLVDLLHDTGFEPSKGAAKRLIQGNGCKINDNVVNNIDHVINFESFKDQPF
IKLSAGKKRHIKVVVD

Sequences:

>Translated_416_residues
MKHASEFLNFIQERGYLYQCTNIEGLDQLLLQSNYIVAYIGFDCTAPSLHAGHLIQIMMLCHLQKFGYKPIVLLGGGTTK
IGDPSFKDKARSILPVENINQNTSSIRRILEKMVSFNDSKTGAMIVNNADWLDNIKYIDFLRDIGAYFSVNRMLSFDSVK
TRLDREQTLSFLEFNYMLLQAYDFIELNKKYDCRLQIGGSDQWGNIVNGIELGKKLNLPELFGLTMPLLLNAQGKKMGKT
ESGAVWLDDNMLKPYDYWQYFRNVDDQDVGRFLRLLTDVPIDEIRKLESLKDQEINEAKKVLATEVTKICHGDKEAELAQ
FAAISAFENEDSSLLPDYIIKKEQVASGISLVDLLHDTGFEPSKGAAKRLIQGNGCKINDNVVNNIDHVINFESFKDQPF
IKLSAGKKRHIKVVVD
>Mature_416_residues
MKHASEFLNFIQERGYLYQCTNIEGLDQLLLQSNYIVAYIGFDCTAPSLHAGHLIQIMMLCHLQKFGYKPIVLLGGGTTK
IGDPSFKDKARSILPVENINQNTSSIRRILEKMVSFNDSKTGAMIVNNADWLDNIKYIDFLRDIGAYFSVNRMLSFDSVK
TRLDREQTLSFLEFNYMLLQAYDFIELNKKYDCRLQIGGSDQWGNIVNGIELGKKLNLPELFGLTMPLLLNAQGKKMGKT
ESGAVWLDDNMLKPYDYWQYFRNVDDQDVGRFLRLLTDVPIDEIRKLESLKDQEINEAKKVLATEVTKICHGDKEAELAQ
FAAISAFENEDSSLLPDYIIKKEQVASGISLVDLLHDTGFEPSKGAAKRLIQGNGCKINDNVVNNIDHVINFESFKDQPF
IKLSAGKKRHIKVVVD

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=372, Percent_Identity=35.2150537634409, Blast_Score=212, Evalue=4e-55,
Organism=Escherichia coli, GI1787925, Length=418, Percent_Identity=39.9521531100478, Blast_Score=298, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI25145802, Length=306, Percent_Identity=36.2745098039216, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI25145805, Length=245, Percent_Identity=34.6938775510204, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6325160, Length=332, Percent_Identity=34.9397590361446, Blast_Score=195, Evalue=1e-50,
Organism=Drosophila melanogaster, GI20130383, Length=409, Percent_Identity=34.4743276283619, Blast_Score=237, Evalue=9e-63,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_WOLPM (Q73G50)

Other databases:

- EMBL:   AE017196
- RefSeq:   NP_966834.1
- ProteinModelPortal:   Q73G50
- SMR:   Q73G50
- EnsemblBacteria:   EBWOLT00000004798
- GeneID:   2738203
- GenomeReviews:   AE017196_GR
- KEGG:   wol:WD1115
- TIGR:   WD_1115
- GeneTree:   EBGT00050000031319
- HOGENOM:   HBG288125
- OMA:   WINQDDR
- PhylomeDB:   Q73G50
- ProtClustDB:   PRK13354
- BioCyc:   WPIP955:WD_1115-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 47230; Mature: 47230

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 39-39 BINDING 176-176 BINDING 180-180 BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHASEFLNFIQERGYLYQCTNIEGLDQLLLQSNYIVAYIGFDCTAPSLHAGHLIQIMML
CCCHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHH
CHLQKFGYKPIVLLGGGTTKIGDPSFKDKARSILPVENINQNTSSIRRILEKMVSFNDSK
HHHHHCCCCEEEEECCCCCCCCCCCHHHHHCCCCCHHCCCCCHHHHHHHHHHHHCCCCCC
TGAMIVNNADWLDNIKYIDFLRDIGAYFSVNRMLSFDSVKTRLDREQTLSFLEFNYMLLQ
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
AYDFIELNKKYDCRLQIGGSDQWGNIVNGIELGKKLNLPELFGLTMPLLLNAQGKKMGKT
HHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHCHHHHHCCCCCCCCCCC
ESGAVWLDDNMLKPYDYWQYFRNVDDQDVGRFLRLLTDVPIDEIRKLESLKDQEINEAKK
CCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VLATEVTKICHGDKEAELAQFAAISAFENEDSSLLPDYIIKKEQVASGISLVDLLHDTGF
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCC
EPSKGAAKRLIQGNGCKINDNVVNNIDHVINFESFKDQPFIKLSAGKKRHIKVVVD
CCCHHHHHHHHCCCCCEECCHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEEEEC
>Mature Secondary Structure
MKHASEFLNFIQERGYLYQCTNIEGLDQLLLQSNYIVAYIGFDCTAPSLHAGHLIQIMML
CCCHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHH
CHLQKFGYKPIVLLGGGTTKIGDPSFKDKARSILPVENINQNTSSIRRILEKMVSFNDSK
HHHHHCCCCEEEEECCCCCCCCCCCHHHHHCCCCCHHCCCCCHHHHHHHHHHHHCCCCCC
TGAMIVNNADWLDNIKYIDFLRDIGAYFSVNRMLSFDSVKTRLDREQTLSFLEFNYMLLQ
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
AYDFIELNKKYDCRLQIGGSDQWGNIVNGIELGKKLNLPELFGLTMPLLLNAQGKKMGKT
HHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHCHHHHHCCCCCCCCCCC
ESGAVWLDDNMLKPYDYWQYFRNVDDQDVGRFLRLLTDVPIDEIRKLESLKDQEINEAKK
CCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VLATEVTKICHGDKEAELAQFAAISAFENEDSSLLPDYIIKKEQVASGISLVDLLHDTGF
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCC
EPSKGAAKRLIQGNGCKINDNVVNNIDHVINFESFKDQPFIKLSAGKKRHIKVVVD
CCCHHHHHHHHCCCCCEECCHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA