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The map label for this gene is cisA [H]

Identifier: 225630928

GI number: 225630928

Start: 1337329

End: 1339050

Strand: Reverse

Name: cisA [H]

Synonym: WRi_012450

Alternate gene names: 225630928

Gene position: 1339050-1337329 (Counterclockwise)

Preceding gene: 225630929

Following gene: 225630926

Centisome position: 92.61

GC content: 34.2

Gene sequence:

>1722_bases
ATGACAACAGTAGCATTATATGCGAGAGTTTCATCGAAGAAACAAGCGCAGAACAATACAATAGAGAGCCAAATTGCCGA
GCTCAAGTGTAGAATTGCTGCGGATAAACATGAGTTATTAGATGAGTATGAATTTAAGGACAATGGCATTAGTGGGTGGA
TTTTAGGGCGTGAAGGTTTAGAAGCGTTACGTGATAAAGTAGCAGAAGGTGAAATTGATAAAATTTATATTCATTCACCT
GACCGGTTGTCAAGAAAATCTGCACACCAAGTGGTATTACTTGAAGAGTTTGAGAAAGCAGGAGTAGAGGTAATATTCTC
AAATCATAAGATTGAAAATAATCCAGAGTCTAAATTATTGCTAGGAATGCAAGGATTATTGTCAGAGTACGAATGTACAA
AGACTATGGAACGTAGTCGTAGAGGAAAACGCCATAGGGCAAGAAAAGGTTGTATAAGTGTAATTGGTATTGCGCCTTTT
GGTTATAAACGTATGAAGCATGTAGATAGAGAAAAGACAAGTTTTGAAATCAATGAAGAAGAAGCAAAAATAGTAAGGCA
AATATTCATGTGGATAGGACAAGAAAGAATAAGTATAAGGGAAGCTATACGTAGACTAAAAGATATGTCAGTAAGAACAA
GAACTGAAAAGAAGGTTTGGCGTCCAATAATAATTTGGAAGATGTTAAGAAACCCAACGTATAAGGGACAAGCAGCGTTT
GGTAAGTTAAAAAGGGTTGAAAGAATATCAAGAAAAAAAAGTAAACAAAAGGTAAAAAATGTTTATGTTTATCGTACAGA
TGAAGAAAGTTGGATTTATATACCAGTACCAAAGATAGTTGATGAAGGATTATTTAACAAAGTACAAAAGCAACTGGATG
AAAATAGAAAAAGAGCAAGAATGCAGAGAAGGGAAGAGATAAATCTACTACAAGGTCTAACTATATGTCAAAACTGCAAA
AGTACGTATAGTGGTGTGCATCATAGAGATGGGGAAAAAACGTATAGCTATTATCGCTGTAGTAGTATAGTACGTATTAC
TGATGATGAGGAAAAATGTAACAATAAATTAGTCCGAGCAGATATGTTAGAAATAGCTGTATGGGAAAAGGTGAAAGATG
TACTAAAAAACCCAGAGATGATAAAAAAGGAATACCAACGTAGAGTATTAGAAAATAAAAATGATGAATCATCAGAGAAA
AAGTTTGCAAGAAGAAAAAATCAAATAAAAGAAGGTATCGAAAAATTAATGGAAGATTATTATAGTCAAGAAAATGCAGG
AGAGAAAGGATATATAAGCGAGGAAGAATTTAAACAGACAATGAAAAAAATGAAGGAACGCTTACAAGGGATAGAAGAAG
AGAAAAAAAAGGTAGTTGATCAGAAGGCAGTAGAAAAAGGAATAAGTCTTATTATAAATAGTATAAAGAATTTTTATTCT
AGTGTAAAATCGAACCTGGAACAGCTAGATTGGCAAACTAAGCGCGGCATTATTAAAGCATTAATAGGACAAATTAACAT
TGGTCGTGATCAGGTAGAAGTAGGATTTCAGATCGAAGAACCAGAGCAGGATGGGGGAATTCTTAATTTGTATCATTGTA
CTACACATCATGATAAGGGGGCTGGCGGAGTTTGTCAAGCAAGTTTTTTCGTTTCTATTTCCAATAAAAGTTCGTTATAT
GGTTATGCAAGAAGTCTAATGAATCGCGGCATGACGTCTTAG

Upstream 100 bases:

>100_bases
TATGAACCTGATGAGTTAGCAGAGCAACATCTAGCAGACGCTTATGAGCTGTTACTAAGATATGCGGTAAAAAGAAACAA
CGAAAAGGAGATGAAAGAAA

Downstream 100 bases:

>100_bases
CTATAGATGCTGGTCTGTGTGATTTCAAGAGAAGCTTAGTAGATAAGTTGTGTTTTAAACCAGATTCGGTATTTTAAACT
GAACATTTTCAATAATACCA

Product: Putative site-specific recombinase

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MTTVALYARVSSKKQAQNNTIESQIAELKCRIAADKHELLDEYEFKDNGISGWILGREGLEALRDKVAEGEIDKIYIHSP
DRLSRKSAHQVVLLEEFEKAGVEVIFSNHKIENNPESKLLLGMQGLLSEYECTKTMERSRRGKRHRARKGCISVIGIAPF
GYKRMKHVDREKTSFEINEEEAKIVRQIFMWIGQERISIREAIRRLKDMSVRTRTEKKVWRPIIIWKMLRNPTYKGQAAF
GKLKRVERISRKKSKQKVKNVYVYRTDEESWIYIPVPKIVDEGLFNKVQKQLDENRKRARMQRREEINLLQGLTICQNCK
STYSGVHHRDGEKTYSYYRCSSIVRITDDEEKCNNKLVRADMLEIAVWEKVKDVLKNPEMIKKEYQRRVLENKNDESSEK
KFARRKNQIKEGIEKLMEDYYSQENAGEKGYISEEEFKQTMKKMKERLQGIEEEKKKVVDQKAVEKGISLIINSIKNFYS
SVKSNLEQLDWQTKRGIIKALIGQINIGRDQVEVGFQIEEPEQDGGILNLYHCTTHHDKGAGGVCQASFFVSISNKSSLY
GYARSLMNRGMTS

Sequences:

>Translated_573_residues
MTTVALYARVSSKKQAQNNTIESQIAELKCRIAADKHELLDEYEFKDNGISGWILGREGLEALRDKVAEGEIDKIYIHSP
DRLSRKSAHQVVLLEEFEKAGVEVIFSNHKIENNPESKLLLGMQGLLSEYECTKTMERSRRGKRHRARKGCISVIGIAPF
GYKRMKHVDREKTSFEINEEEAKIVRQIFMWIGQERISIREAIRRLKDMSVRTRTEKKVWRPIIIWKMLRNPTYKGQAAF
GKLKRVERISRKKSKQKVKNVYVYRTDEESWIYIPVPKIVDEGLFNKVQKQLDENRKRARMQRREEINLLQGLTICQNCK
STYSGVHHRDGEKTYSYYRCSSIVRITDDEEKCNNKLVRADMLEIAVWEKVKDVLKNPEMIKKEYQRRVLENKNDESSEK
KFARRKNQIKEGIEKLMEDYYSQENAGEKGYISEEEFKQTMKKMKERLQGIEEEKKKVVDQKAVEKGISLIINSIKNFYS
SVKSNLEQLDWQTKRGIIKALIGQINIGRDQVEVGFQIEEPEQDGGILNLYHCTTHHDKGAGGVCQASFFVSISNKSSLY
GYARSLMNRGMTS
>Mature_572_residues
TTVALYARVSSKKQAQNNTIESQIAELKCRIAADKHELLDEYEFKDNGISGWILGREGLEALRDKVAEGEIDKIYIHSPD
RLSRKSAHQVVLLEEFEKAGVEVIFSNHKIENNPESKLLLGMQGLLSEYECTKTMERSRRGKRHRARKGCISVIGIAPFG
YKRMKHVDREKTSFEINEEEAKIVRQIFMWIGQERISIREAIRRLKDMSVRTRTEKKVWRPIIIWKMLRNPTYKGQAAFG
KLKRVERISRKKSKQKVKNVYVYRTDEESWIYIPVPKIVDEGLFNKVQKQLDENRKRARMQRREEINLLQGLTICQNCKS
TYSGVHHRDGEKTYSYYRCSSIVRITDDEEKCNNKLVRADMLEIAVWEKVKDVLKNPEMIKKEYQRRVLENKNDESSEKK
FARRKNQIKEGIEKLMEDYYSQENAGEKGYISEEEFKQTMKKMKERLQGIEEEKKKVVDQKAVEKGISLIINSIKNFYSS
VKSNLEQLDWQTKRGIIKALIGQINIGRDQVEVGFQIEEPEQDGGILNLYHCTTHHDKGAGGVCQASFFVSISNKSSLYG
YARSLMNRGMTS

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 66776; Mature: 66644

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVALYARVSSKKQAQNNTIESQIAELKCRIAADKHELLDEYEFKDNGISGWILGREGL
CCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECHHHH
EALRDKVAEGEIDKIYIHSPDRLSRKSAHQVVLLEEFEKAGVEVIFSNHKIENNPESKLL
HHHHHHHCCCCCCEEEEECCHHHHHHHHHHEHHHHHHHHCCEEEEEECCCCCCCCCHHHH
LGMQGLLSEYECTKTMERSRRGKRHRARKGCISVIGIAPFGYKRMKHVDREKTSFEINEE
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCEECHH
EAKIVRQIFMWIGQERISIREAIRRLKDMSVRTRTEKKVWRPIIIWKMLRNPTYKGQAAF
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
GKLKRVERISRKKSKQKVKNVYVYRTDEESWIYIPVPKIVDEGLFNKVQKQLDENRKRAR
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
MQRREEINLLQGLTICQNCKSTYSGVHHRDGEKTYSYYRCSSIVRITDDEEKCNNKLVRA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEEECCCHHHHCCHHHHH
DMLEIAVWEKVKDVLKNPEMIKKEYQRRVLENKNDESSEKKFARRKNQIKEGIEKLMEDY
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YSQENAGEKGYISEEEFKQTMKKMKERLQGIEEEKKKVVDQKAVEKGISLIINSIKNFYS
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVKSNLEQLDWQTKRGIIKALIGQINIGRDQVEVGFQIEEPEQDGGILNLYHCTTHHDKG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECEEECCCCCCCCEEEEEEEECCCCCC
AGGVCQASFFVSISNKSSLYGYARSLMNRGMTS
CCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTVALYARVSSKKQAQNNTIESQIAELKCRIAADKHELLDEYEFKDNGISGWILGREGL
CCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECHHHH
EALRDKVAEGEIDKIYIHSPDRLSRKSAHQVVLLEEFEKAGVEVIFSNHKIENNPESKLL
HHHHHHHCCCCCCEEEEECCHHHHHHHHHHEHHHHHHHHCCEEEEEECCCCCCCCCHHHH
LGMQGLLSEYECTKTMERSRRGKRHRARKGCISVIGIAPFGYKRMKHVDREKTSFEINEE
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCEECHH
EAKIVRQIFMWIGQERISIREAIRRLKDMSVRTRTEKKVWRPIIIWKMLRNPTYKGQAAF
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
GKLKRVERISRKKSKQKVKNVYVYRTDEESWIYIPVPKIVDEGLFNKVQKQLDENRKRAR
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
MQRREEINLLQGLTICQNCKSTYSGVHHRDGEKTYSYYRCSSIVRITDDEEKCNNKLVRA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEEECCCHHHHCCHHHHH
DMLEIAVWEKVKDVLKNPEMIKKEYQRRVLENKNDESSEKKFARRKNQIKEGIEKLMEDY
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YSQENAGEKGYISEEEFKQTMKKMKERLQGIEEEKKKVVDQKAVEKGISLIINSIKNFYS
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVKSNLEQLDWQTKRGIIKALIGQINIGRDQVEVGFQIEEPEQDGGILNLYHCTTHHDKG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECEEECCCCCCCCEEEEEEEECCCCCC
AGGVCQASFFVSISNKSSLYGYARSLMNRGMTS
CCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]