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The map label for this gene is ygfA [C]

Identifier: 225630922

GI number: 225630922

Start: 1331813

End: 1332364

Strand: Direct

Name: ygfA [C]

Synonym: WRi_012390

Alternate gene names: 225630922

Gene position: 1331813-1332364 (Clockwise)

Preceding gene: 225630921

Following gene: 225630924

Centisome position: 92.11

GC content: 30.98

Gene sequence:

>552_bases
ATGTTCAAAGATATTAAACAGCACAAAAAGGAAATAAGAGAGCAATATAGAGCTATAAGAAAAGATATTGATGAAAGTTA
TTCCAGTTATGCAGCAAATTCCCTTATTAATCTCTTTAATCAGAACTTAAGCTACGTTAAAGGCAAAACAATTGCGGCTT
ACATTCCAATGGATGGGGAAATAGATGTTGTGCCTTTGATGCATAGTTTACTCGATTTAGATTATAAAGTAGCAATTCCT
AATAAAAATAAGTTACTAAAATTTGAGGAATGGAACAAAACAAATGAAGACGTAATTCCTGACACAATCATTACTCCTGT
TATTGCTTTTGATGATCATTTTAATAGATTAGGTTTTGGCGGTGGTTGGTATGATACAATGATAAAAAAATTACGGCCGC
TTGGAAAAATATTTATAGGTGTAGCCTATGAGAAACAATATTGTAAAAATCTACCTGTAGAACAACATGATCAAAAATTG
GATATTATAATCACTGAAATGTGTGTTAGATTTGAAAGTGGATTGCTCAAAAAAGTGGATAAAAAGGAGTAG

Upstream 100 bases:

>100_bases
AATCAAGACCAGGATATGGAGTTTATTGACTTTGACTTTTTAAGTGAGCGCTTGAAGCAAAATAGTTTACAAGAAAAACT
TACTGCGAAATGGATAGATC

Downstream 100 bases:

>100_bases
CGCAATCCCTCTTTCTGATAGTAATTTTTGCGTCAAAAAATGCATCAACTCGGTCCTGTGATTCCTCCTTCACTAACTCG
TGCAGCACTTGAAATACCTA

Product: 5-formyltetrahydrofolate cyclo-ligase, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MFKDIKQHKKEIREQYRAIRKDIDESYSSYAANSLINLFNQNLSYVKGKTIAAYIPMDGEIDVVPLMHSLLDLDYKVAIP
NKNKLLKFEEWNKTNEDVIPDTIITPVIAFDDHFNRLGFGGGWYDTMIKKLRPLGKIFIGVAYEKQYCKNLPVEQHDQKL
DIIITEMCVRFESGLLKKVDKKE

Sequences:

>Translated_183_residues
MFKDIKQHKKEIREQYRAIRKDIDESYSSYAANSLINLFNQNLSYVKGKTIAAYIPMDGEIDVVPLMHSLLDLDYKVAIP
NKNKLLKFEEWNKTNEDVIPDTIITPVIAFDDHFNRLGFGGGWYDTMIKKLRPLGKIFIGVAYEKQYCKNLPVEQHDQKL
DIIITEMCVRFESGLLKKVDKKE
>Mature_183_residues
MFKDIKQHKKEIREQYRAIRKDIDESYSSYAANSLINLFNQNLSYVKGKTIAAYIPMDGEIDVVPLMHSLLDLDYKVAIP
NKNKLLKFEEWNKTNEDVIPDTIITPVIAFDDHFNRLGFGGGWYDTMIKKLRPLGKIFIGVAYEKQYCKNLPVEQHDQKL
DIIITEMCVRFESGLLKKVDKKE

Specific function: Unknown

COG id: COG0212

COG function: function code H; 5-formyltetrahydrofolate cyclo-ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family [H]

Homologues:

Organism=Escherichia coli, GI1789278, Length=178, Percent_Identity=27.5280898876405, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002698 [H]

Pfam domain/function: PF01812 5-FTHF_cyc-lig [H]

EC number: NA

Molecular weight: Translated: 21320; Mature: 21320

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKDIKQHKKEIREQYRAIRKDIDESYSSYAANSLINLFNQNLSYVKGKTIAAYIPMDGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCC
IDVVPLMHSLLDLDYKVAIPNKNKLLKFEEWNKTNEDVIPDTIITPVIAFDDHFNRLGFG
CHHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHCCCC
GGWYDTMIKKLRPLGKIFIGVAYEKQYCKNLPVEQHDQKLDIIITEMCVRFESGLLKKVD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
KKE
CCC
>Mature Secondary Structure
MFKDIKQHKKEIREQYRAIRKDIDESYSSYAANSLINLFNQNLSYVKGKTIAAYIPMDGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCC
IDVVPLMHSLLDLDYKVAIPNKNKLLKFEEWNKTNEDVIPDTIITPVIAFDDHFNRLGFG
CHHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHCCCC
GGWYDTMIKKLRPLGKIFIGVAYEKQYCKNLPVEQHDQKLDIIITEMCVRFESGLLKKVD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
KKE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]