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The map label for this gene is hemH [H]

Identifier: 225630852

GI number: 225630852

Start: 1248371

End: 1249318

Strand: Direct

Name: hemH [H]

Synonym: WRi_011580

Alternate gene names: 225630852

Gene position: 1248371-1249318 (Clockwise)

Preceding gene: 225630851

Following gene: 225630853

Centisome position: 86.34

GC content: 36.39

Gene sequence:

>948_bases
GTGAAAAAGGCAGTTATCTTATTTAATCTTGGCGGGCCTGATTCACTAAATGCGGTCCGTCCTTTTTTATTCAACCTTTT
TTATGACAGGAGAATAATCAATCTACCGAATCCCTTTCGGTTTCTTTTAGCAAAGTTTATATCTGCGAAACGAGAAAATA
CTGCACGAAAAATATACGAGGAAATTGGAGGTAAATCGCCAATTCTAGAGAATACAAAAATGCAAGCTAATGCTTTAGAA
CTAAAATTAAACGAAAATAGAAACCATGTACACAAGGTATTCATCTGCATGCGCTATTGGCGTCCATTTGCTGACGAAGT
TATTGAAAGTGTAAAACAGTTTGATCCTGACGAAGTAATTCTGCTGCCACTGTATCCACAATATTCAACTACCACAACTC
TGTCATCCATCGAGAATTGGCAAAAAAATGCTAAACGGTATGGCCTAAAATGCAATACAAAAATGATTCACCGTTATTAT
GATAACCAAGATTTTATTGAAGCTCACACTAATCTGATAGCTAAGTACTATAAATTGGCTAGAAAAATCGGTAAACCAAG
AGTCCTATTTTCAGCCCATAGCTTACCTCTTAGTATCATTAAAAAGGGCGACCCTTACGCTTCACAGGTAGAAAGAAGCG
TAGAGTTAATAGTAGAAAAGTTAGCTATCAATAACTTAGATTGGTCAATATGTTATCAGAGTAAGATTGGTCCTGTAAAA
TGGCTAGAGCCAAGCACTGAAAGTGAGCTATTACGCGCAAAAGCTGATGGTGTACCTGTAGTTCTATCACCTATATCTTT
TGTTTCTGAGCATTCAGAAACTCTAGTTGAACTTGATATAGAATATAAAGCAATTATCAAGGATGGATATTACTTTCGTG
TGCCAACTCTCAGTACCGATCCCTTGTTTATAAAATGCTTAGCCGATTTGTGCATAAATCTCCCTTAA

Upstream 100 bases:

>100_bases
GTATGCAAATGTGAGAAATTTAGTGCCTATAACTAAATTTGATGAGTGTGTAGCTAATTTTATAGGTACCCCGAAAGTAA
CTTGTTGTAGTAGTGCGTAG

Downstream 100 bases:

>100_bases
AGTCCTGATTCATAGGGCTTTTCTTTTAGGATTTTATGTGATAGAATAACAGGAAGTTATTAACTATTTCATGCCAAACA
AGCTAAAAAAGAAATCTAAC

Product: ferrochelatase

Products: NA

Alternate protein names: Heme synthase; Protoheme ferro-lyase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKKAVILFNLGGPDSLNAVRPFLFNLFYDRRIINLPNPFRFLLAKFISAKRENTARKIYEEIGGKSPILENTKMQANALE
LKLNENRNHVHKVFICMRYWRPFADEVIESVKQFDPDEVILLPLYPQYSTTTTLSSIENWQKNAKRYGLKCNTKMIHRYY
DNQDFIEAHTNLIAKYYKLARKIGKPRVLFSAHSLPLSIIKKGDPYASQVERSVELIVEKLAINNLDWSICYQSKIGPVK
WLEPSTESELLRAKADGVPVVLSPISFVSEHSETLVELDIEYKAIIKDGYYFRVPTLSTDPLFIKCLADLCINLP

Sequences:

>Translated_315_residues
MKKAVILFNLGGPDSLNAVRPFLFNLFYDRRIINLPNPFRFLLAKFISAKRENTARKIYEEIGGKSPILENTKMQANALE
LKLNENRNHVHKVFICMRYWRPFADEVIESVKQFDPDEVILLPLYPQYSTTTTLSSIENWQKNAKRYGLKCNTKMIHRYY
DNQDFIEAHTNLIAKYYKLARKIGKPRVLFSAHSLPLSIIKKGDPYASQVERSVELIVEKLAINNLDWSICYQSKIGPVK
WLEPSTESELLRAKADGVPVVLSPISFVSEHSETLVELDIEYKAIIKDGYYFRVPTLSTDPLFIKCLADLCINLP
>Mature_315_residues
MKKAVILFNLGGPDSLNAVRPFLFNLFYDRRIINLPNPFRFLLAKFISAKRENTARKIYEEIGGKSPILENTKMQANALE
LKLNENRNHVHKVFICMRYWRPFADEVIESVKQFDPDEVILLPLYPQYSTTTTLSSIENWQKNAKRYGLKCNTKMIHRYY
DNQDFIEAHTNLIAKYYKLARKIGKPRVLFSAHSLPLSIIKKGDPYASQVERSVELIVEKLAINNLDWSICYQSKIGPVK
WLEPSTESELLRAKADGVPVVLSPISFVSEHSETLVELDIEYKAIIKDGYYFRVPTLSTDPLFIKCLADLCINLP

Specific function: Catalyzes the ferrous insertion into protoporphyrin IX [H]

COG id: COG0276

COG function: function code H; Protoheme ferro-lyase (ferrochelatase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ferrochelatase family [H]

Homologues:

Organism=Homo sapiens, GI60499021, Length=321, Percent_Identity=34.8909657320872, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI60499025, Length=321, Percent_Identity=34.8909657320872, Blast_Score=158, Evalue=7e-39,
Organism=Escherichia coli, GI1786681, Length=320, Percent_Identity=26.5625, Blast_Score=87, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324750, Length=322, Percent_Identity=31.9875776397516, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17864156, Length=322, Percent_Identity=32.2981366459627, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24651774, Length=257, Percent_Identity=31.1284046692607, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45552018, Length=128, Percent_Identity=39.0625, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001015
- InterPro:   IPR019772 [H]

Pfam domain/function: PF00762 Ferrochelatase [H]

EC number: =4.99.1.1 [H]

Molecular weight: Translated: 36351; Mature: 36351

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00534 FERROCHELATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAVILFNLGGPDSLNAVRPFLFNLFYDRRIINLPNPFRFLLAKFISAKRENTARKIYE
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
EIGGKSPILENTKMQANALELKLNENRNHVHKVFICMRYWRPFADEVIESVKQFDPDEVI
HHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEE
LLPLYPQYSTTTTLSSIENWQKNAKRYGLKCNTKMIHRYYDNQDFIEAHTNLIAKYYKLA
EEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RKIGKPRVLFSAHSLPLSIIKKGDPYASQVERSVELIVEKLAINNLDWSICYQSKIGPVK
HHHCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEE
WLEPSTESELLRAKADGVPVVLSPISFVSEHSETLVELDIEYKAIIKDGYYFRVPTLSTD
ECCCCCHHHHHHHHCCCCCEEECHHHHHHHCCCEEEEEECEEEEEEECCEEEECCCCCCC
PLFIKCLADLCINLP
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKAVILFNLGGPDSLNAVRPFLFNLFYDRRIINLPNPFRFLLAKFISAKRENTARKIYE
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
EIGGKSPILENTKMQANALELKLNENRNHVHKVFICMRYWRPFADEVIESVKQFDPDEVI
HHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEE
LLPLYPQYSTTTTLSSIENWQKNAKRYGLKCNTKMIHRYYDNQDFIEAHTNLIAKYYKLA
EEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RKIGKPRVLFSAHSLPLSIIKKGDPYASQVERSVELIVEKLAINNLDWSICYQSKIGPVK
HHHCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEE
WLEPSTESELLRAKADGVPVVLSPISFVSEHSETLVELDIEYKAIIKDGYYFRVPTLSTD
ECCCCCHHHHHHHHCCCCCEEECHHHHHHHCCCEEEEEECEEEEEEECCEEEECCCCCCC
PLFIKCLADLCINLP
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA