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The map label for this gene is hisS [H]

Identifier: 225630808

GI number: 225630808

Start: 1200794

End: 1202020

Strand: Direct

Name: hisS [H]

Synonym: WRi_011120

Alternate gene names: 225630808

Gene position: 1200794-1202020 (Clockwise)

Preceding gene: 225630807

Following gene: 225630817

Centisome position: 83.05

GC content: 35.29

Gene sequence:

>1227_bases
ATGACTAATCAAACGGTTAGAGGAACAAAAGATTTCCTGTTTGATGAATGGTACAAGTTTAAACACATAGAGCAAACAGC
AAGCAGAATCTCAAATCTATATGGCTTTTTGCCTGCTCAAACTCCGATATTTGAATACACGGAGGTTTTTACAAAAACCT
TAGGTGATAGCTCAGATATCATCACTAAAGAGATGTATAGCTTCAATGATAAAGGGGGAAAGAGCATAACTTTACGTCCT
GAGTTCACTGCAGCGATTGTCAGGCTACTCATTGAAAAAAAACTGCAAACACCGATAAAATTATTCTCAACAGGACCTGC
ATTTCGCTATGAAAGACCGCAAAAGGGAAGACAAAGGCAATTTCATCAGATAAATTTTGAGGTTTTTGGCATAGAAGATC
CAAAAGCTGATATTGAATTGATATCACTCGTTCAGCACTTACTAACTGAATTTGGCATCAATAAAAATGTAAAGCTGGAA
ATCAACTCTTTAGGTGATGGTGAAACAATAACTAAGTATAGAGAAGCTTTGATCTTATACTTTACAAAGTATCAAAATGA
TCTATCAGAAGATAGCAAAAATAGGTTGATCAAAAACCCACTCAGAATACTGGATTCCAAGGATGAGAAAGATAAATCAA
TAATTTCTGATGCGCCTAAAATCAGCAATTATTATACGAAAGGGTCCTCAGATTTCTTTGAACAAATACTAAATGGATTA
ACAATTCTTGATATACCTTATACCGTAAACAATAAACTAGTTCGAGGTTTGGATTATTATTGCCACACAGTGTTTGAGTT
TGTTACAGAGGATTTAGGTGCACAAGGGGCAGTTTTTGCCGGTGGAAGATACGATAATTTAGTATCTTCAGTAGGTGGAA
AACACACTCCAGCAATAGGATTTGCAGGGGGTATTGAGCGCATAATGGAGTTAATCAATTATTCTCCGAAAGAAGAGCGA
CCTATTTACCTAATTCCAATCGGTAGAGAGGCTGAAAAACATGCTTTAACACTTGCGAATGAATTGCGCAGAAATGGTTT
ATATGTAATCTATGAATATAGCGGAACGCTCAGAACCCGAATGAAAAAAGCAAATCAAGCAAATGCTAAAGCTGCTTTAA
TCTTTGGCGATGAAGAATTGAGTAGTAAAACTTTAAAGATTAAAAACATGAATACAGGTGAAGAAAAAATAATTGCTCGC
GATAACACAATAGAAAATATCTATTAG

Upstream 100 bases:

>100_bases
CTTGCTCTGGATGCCCAAGTGCTGCAATTACTTTAAAGCAAGGAGTGCAAAATATGCTGAGCTACCACATACCAGAGATT
GCAGGTATAGAGACCACACT

Downstream 100 bases:

>100_bases
GAGCAATGCAAATGTTTTTCTCAGTCAGCTATAGAAGAAAATTCGTCTGCACAAGAATTTTTCATAGTATTCAATGTATC
ATAATCTGTAAAATCTAATT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MTNQTVRGTKDFLFDEWYKFKHIEQTASRISNLYGFLPAQTPIFEYTEVFTKTLGDSSDIITKEMYSFNDKGGKSITLRP
EFTAAIVRLLIEKKLQTPIKLFSTGPAFRYERPQKGRQRQFHQINFEVFGIEDPKADIELISLVQHLLTEFGINKNVKLE
INSLGDGETITKYREALILYFTKYQNDLSEDSKNRLIKNPLRILDSKDEKDKSIISDAPKISNYYTKGSSDFFEQILNGL
TILDIPYTVNNKLVRGLDYYCHTVFEFVTEDLGAQGAVFAGGRYDNLVSSVGGKHTPAIGFAGGIERIMELINYSPKEER
PIYLIPIGREAEKHALTLANELRRNGLYVIYEYSGTLRTRMKKANQANAKAALIFGDEELSSKTLKIKNMNTGEEKIIAR
DNTIENIY

Sequences:

>Translated_408_residues
MTNQTVRGTKDFLFDEWYKFKHIEQTASRISNLYGFLPAQTPIFEYTEVFTKTLGDSSDIITKEMYSFNDKGGKSITLRP
EFTAAIVRLLIEKKLQTPIKLFSTGPAFRYERPQKGRQRQFHQINFEVFGIEDPKADIELISLVQHLLTEFGINKNVKLE
INSLGDGETITKYREALILYFTKYQNDLSEDSKNRLIKNPLRILDSKDEKDKSIISDAPKISNYYTKGSSDFFEQILNGL
TILDIPYTVNNKLVRGLDYYCHTVFEFVTEDLGAQGAVFAGGRYDNLVSSVGGKHTPAIGFAGGIERIMELINYSPKEER
PIYLIPIGREAEKHALTLANELRRNGLYVIYEYSGTLRTRMKKANQANAKAALIFGDEELSSKTLKIKNMNTGEEKIIAR
DNTIENIY
>Mature_407_residues
TNQTVRGTKDFLFDEWYKFKHIEQTASRISNLYGFLPAQTPIFEYTEVFTKTLGDSSDIITKEMYSFNDKGGKSITLRPE
FTAAIVRLLIEKKLQTPIKLFSTGPAFRYERPQKGRQRQFHQINFEVFGIEDPKADIELISLVQHLLTEFGINKNVKLEI
NSLGDGETITKYREALILYFTKYQNDLSEDSKNRLIKNPLRILDSKDEKDKSIISDAPKISNYYTKGSSDFFEQILNGLT
ILDIPYTVNNKLVRGLDYYCHTVFEFVTEDLGAQGAVFAGGRYDNLVSSVGGKHTPAIGFAGGIERIMELINYSPKEERP
IYLIPIGREAEKHALTLANELRRNGLYVIYEYSGTLRTRMKKANQANAKAALIFGDEELSSKTLKIKNMNTGEEKIIARD
NTIENIY

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=461, Percent_Identity=24.9457700650759, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI15029520, Length=461, Percent_Identity=24.5119305856833, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1788861, Length=414, Percent_Identity=39.8550724637681, Blast_Score=327, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI71993686, Length=462, Percent_Identity=25.974025974026, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71993693, Length=462, Percent_Identity=25.974025974026, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325290, Length=456, Percent_Identity=25.6578947368421, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24643061, Length=457, Percent_Identity=23.8512035010941, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24643059, Length=476, Percent_Identity=22.8991596638655, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 46565; Mature: 46433

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQTVRGTKDFLFDEWYKFKHIEQTASRISNLYGFLPAQTPIFEYTEVFTKTLGDSSDI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCH
ITKEMYSFNDKGGKSITLRPEFTAAIVRLLIEKKLQTPIKLFSTGPAFRYERPQKGRQRQ
HHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHH
FHQINFEVFGIEDPKADIELISLVQHLLTEFGINKNVKLEINSLGDGETITKYREALILY
HHEECEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHH
FTKYQNDLSEDSKNRLIKNPLRILDSKDEKDKSIISDAPKISNYYTKGSSDFFEQILNGL
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCC
TILDIPYTVNNKLVRGLDYYCHTVFEFVTEDLGAQGAVFAGGRYDNLVSSVGGKHTPAIG
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCCCCC
FAGGIERIMELINYSPKEERPIYLIPIGREAEKHALTLANELRRNGLYVIYEYSGTLRTR
CHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHH
MKKANQANAKAALIFGDEELSSKTLKIKNMNTGEEKIIARDNTIENIY
HHHHCCCCCCEEEEECCHHHCCCEEEEECCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure 
TNQTVRGTKDFLFDEWYKFKHIEQTASRISNLYGFLPAQTPIFEYTEVFTKTLGDSSDI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCH
ITKEMYSFNDKGGKSITLRPEFTAAIVRLLIEKKLQTPIKLFSTGPAFRYERPQKGRQRQ
HHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHH
FHQINFEVFGIEDPKADIELISLVQHLLTEFGINKNVKLEINSLGDGETITKYREALILY
HHEECEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHH
FTKYQNDLSEDSKNRLIKNPLRILDSKDEKDKSIISDAPKISNYYTKGSSDFFEQILNGL
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCC
TILDIPYTVNNKLVRGLDYYCHTVFEFVTEDLGAQGAVFAGGRYDNLVSSVGGKHTPAIG
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCCCCC
FAGGIERIMELINYSPKEERPIYLIPIGREAEKHALTLANELRRNGLYVIYEYSGTLRTR
CHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHH
MKKANQANAKAALIFGDEELSSKTLKIKNMNTGEEKIIARDNTIENIY
HHHHCCCCCCEEEEECCHHHCCCEEEEECCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA