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The map label for this gene is truA [H]

Identifier: 225630801

GI number: 225630801

Start: 1194349

End: 1195086

Strand: Reverse

Name: truA [H]

Synonym: WRi_011040

Alternate gene names: 225630801

Gene position: 1195086-1194349 (Counterclockwise)

Preceding gene: 225630802

Following gene: 225630800

Centisome position: 82.65

GC content: 36.99

Gene sequence:

>738_bases
GTGCGATACAAAATAACGGTAGAATATAATGGTAGTAGTTTCTCTGGTTGGCAAAAACAGCAACACTCTGCTAACTCAAT
CCAGGAGAAGATAGAAAATGCTATATTTAATTTTAGTGGTGAGAAAATTGCCTTGTACTGTGGTGGAAGGACCGATGCTG
GGGTTCATGCTTTAGGACAAGTTGCTCATTTTGATATGGAAAAGGAATGTGAGCTTTATAGAATAAGAAACGCAATAAAT
TATCATTTAAAATCAATTCCTATAGTTGTGCTTAATGCAGAAGTTGTAGATGATGCGTTTCACGCACGGTTTTCAGCAAA
AAAAAGATATTACGAATACAGAATAGTTAATCGCTATGCTCCTGCCGCTCTGGAGACGGGTTATGTATGGCAAGTGTTTA
GCCCACTTGATGTAAATATTATGCGTGAAGCTGCTAAACATCTACTTGGAAAACATGACCTTTCAAGTTTTCGTTCAAAA
GATTGTCACGCCGCAAATCCGATAAGAACAATTGATGATATCGATATAGTACAAAACGGGAATCATATACACATCAAAAT
ATCTGCTATTTCCTTTTTACATAACCAAGTGAGGATTATTGCGGGTACTTTAGTTGAATTTGGAAAAAATAGAACCAATC
CGCAAGAAATGCTACATATATTAAGCCAATGCAAAAGAAGTGCTGCTGGGGTTACCGCGCCGCCTTTTGGCTTGTATTTG
GTAAAGATAGATTACTAA

Upstream 100 bases:

>100_bases
TTGGACGAACTACTTTATGGGAGTGGCAGCACTGTAGGCTTGACCATAAATTATGTTAAGATAGAAAATGACTATTTTAG
ATAGAGCTTGATGTAATGCA

Downstream 100 bases:

>100_bases
TTTAATAAAAAGGTATCCGTTCAGCGGAGTGGCAGAATAAGGTAGACAAGGTGATCTAAAAAGATAATCATAGAGCAAAA
GTGAAATAATATGGTAAACC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MRYKITVEYNGSSFSGWQKQQHSANSIQEKIENAIFNFSGEKIALYCGGRTDAGVHALGQVAHFDMEKECELYRIRNAIN
YHLKSIPIVVLNAEVVDDAFHARFSAKKRYYEYRIVNRYAPAALETGYVWQVFSPLDVNIMREAAKHLLGKHDLSSFRSK
DCHAANPIRTIDDIDIVQNGNHIHIKISAISFLHNQVRIIAGTLVEFGKNRTNPQEMLHILSQCKRSAAGVTAPPFGLYL
VKIDY

Sequences:

>Translated_245_residues
MRYKITVEYNGSSFSGWQKQQHSANSIQEKIENAIFNFSGEKIALYCGGRTDAGVHALGQVAHFDMEKECELYRIRNAIN
YHLKSIPIVVLNAEVVDDAFHARFSAKKRYYEYRIVNRYAPAALETGYVWQVFSPLDVNIMREAAKHLLGKHDLSSFRSK
DCHAANPIRTIDDIDIVQNGNHIHIKISAISFLHNQVRIIAGTLVEFGKNRTNPQEMLHILSQCKRSAAGVTAPPFGLYL
VKIDY
>Mature_245_residues
MRYKITVEYNGSSFSGWQKQQHSANSIQEKIENAIFNFSGEKIALYCGGRTDAGVHALGQVAHFDMEKECELYRIRNAIN
YHLKSIPIVVLNAEVVDDAFHARFSAKKRYYEYRIVNRYAPAALETGYVWQVFSPLDVNIMREAAKHLLGKHDLSSFRSK
DCHAANPIRTIDDIDIVQNGNHIHIKISAISFLHNQVRIIAGTLVEFGKNRTNPQEMLHILSQCKRSAAGVTAPPFGLYL
VKIDY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=272, Percent_Identity=30.1470588235294, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=39.8373983739837, Blast_Score=170, Evalue=7e-44,
Organism=Drosophila melanogaster, GI116007752, Length=299, Percent_Identity=28.4280936454849, Blast_Score=98, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27811; Mature: 27811

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYKITVEYNGSSFSGWQKQQHSANSIQEKIENAIFNFSGEKIALYCGGRTDAGVHALGQ
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
VAHFDMEKECELYRIRNAINYHLKSIPIVVLNAEVVDDAFHARFSAKKRYYEYRIVNRYA
HHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCCC
PAALETGYVWQVFSPLDVNIMREAAKHLLGKHDLSSFRSKDCHAANPIRTIDDIDIVQNG
CCHHCCCEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCEEECC
NHIHIKISAISFLHNQVRIIAGTLVEFGKNRTNPQEMLHILSQCKRSAAGVTAPPFGLYL
CEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
VKIDY
EEECC
>Mature Secondary Structure
MRYKITVEYNGSSFSGWQKQQHSANSIQEKIENAIFNFSGEKIALYCGGRTDAGVHALGQ
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
VAHFDMEKECELYRIRNAINYHLKSIPIVVLNAEVVDDAFHARFSAKKRYYEYRIVNRYA
HHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCCC
PAALETGYVWQVFSPLDVNIMREAAKHLLGKHDLSSFRSKDCHAANPIRTIDDIDIVQNG
CCHHCCCEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCEEECC
NHIHIKISAISFLHNQVRIIAGTLVEFGKNRTNPQEMLHILSQCKRSAAGVTAPPFGLYL
CEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
VKIDY
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA