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The map label for this gene is dnaX [H]

Identifier: 225630794

GI number: 225630794

Start: 1186567

End: 1188168

Strand: Direct

Name: dnaX [H]

Synonym: WRi_010970

Alternate gene names: 225630794

Gene position: 1186567-1188168 (Clockwise)

Preceding gene: 225630791

Following gene: 225630795

Centisome position: 82.07

GC content: 34.46

Gene sequence:

>1602_bases
ATGAATATAGCATTAAAACATCGTCCTAGTAGCTTCAAAGATCTAGTAGGTCAAGATATATTAGTGCGTGTATTAGAAAA
TGCTTTTATTTTAAACAAAATCCCACAATCTATACTGCTCTCTGGTAGTAGTGGAGTTGGTAAAACCACCACTGCAAGGA
TAATAGCTTTATGTTTGAATTGTTCCCTGGGGCCAATTTTTGAACCTTGCGGATCATGTGAAAATTGTCTAGCAATAAAA
AATTCAAGCCATCCAGATGTAATTGAAATCGATGCAGCAAGTCACACTAGCATTGACGATATTAAAGTAATCCTGGGAGA
TATTTGCTATTCACCTATAAGCTCTAAATTCAAAGTCTACATTATAGATGAAGTACACATGCTATCTAGCAGTGCATTTA
ACGCATTACTTAAAACCCTAGAAGAGCCACCATCTAGTGTAAAGTTCATTTTAGCAACTACGGAAATAAAAAAGATACCA
ATAACTATTATTGCACGTTGTCAAAGATTTGATTTACACAACATTCCTGTAGCTAAAATAGTGGAACGTTTAAATGATGT
TGCACAAAAAGAGAGTTATTCCATTGAAAAGGGAGCATTAGAATTAATTGCACGACACTCTGGAAATTCAATGCGTAACG
CCTTGTTTTTGATGAATCAAGCAGTGCTATATAGCAAAGATGGTGCAATATCCACTAAAAATGTGACAGACATACTTGGC
CTAGTGGATAAAGATATTATATTCGATCTATTAGGGGCAATATTAGAAGGTGATTTACAGAAAGCTCTATCAGTGTTTGA
CAAGGCGGTAAAAACAGCAAACCCACTTAGTATTTTTGAAGGTCTATTGCAAACAATTCAGTTAGTATGCCGTTTTTTGA
TTACAAAAGAGATTGATAATGCAGTTACAGAGCATGAAAAGAATAGGATAAAAGATTTAAGTATAAAGAGATCTTTAATA
TTTTTTTCGAGATTGTGGAAGATGTTGCTTAAAGGTATTCAAGATATAAAAACTTCAACATGCAACGATGTTGCTGCTGA
AATGATGTTAATTAGCCTCTGTTATCTTTCTGATCTGCCTTCTCCGGAACAGGTGATCAAAAAAGTTCTTTCGCAGAATG
TAGAGCAAGGAAATAGACAGGGCAGACCTGTTGCACCTTCCCCCTCTGTCATCCAAGTAGCTGACTACTCGGATCCAAAA
AAATATGTGGAAAGTAAACAACAACCCCACTCTGTCATCCCAGTGCGTGACACTGGGATCCATGTGGAAAATAAACAACA
AAATTACGATTTTGATAAGATTTTGCAACTATTAAGGCGAAGTAACCAAATTTATCTTTACAAACAACTGTGTAATAATC
TACAATTAATAGATTGTAAACCTGGATATTTAAAATTAAAAGCTGTATCTAAGTTGGATAGTAATTTTTGCAATGATTTA
AAAAATTACTTAAACCAGGTGACTCAGCAAGAGTGGGTTATTGCAGTTGATACAGGTTATATAAACAGGGAAGTGAGTAA
TTTAAATTATGCACCTGCAGTTAAGGATATCCTTGACACGTTTAAAGGTGCAAAAGTAGTTAATATAGAAAATAAGGAGT
AA

Upstream 100 bases:

>100_bases
AGGCAAAAACTCTGTAATTTGGTCAGATCAGAAATGAAGCAGCCATATCAGAACCTTTTTGGGTTGCATACCTATTTCAT
TATTTGTTTTATCCGAGGCT

Downstream 100 bases:

>100_bases
GTTGTGGATTTTAGTCAAATAATGAAACAAGCGCAAGAGATGCAAAAAAAGCTTGCAGAAGCTCAAGAAAAATATGTTGG
AAAAGAGTTTCAAGGTATTT

Product: DNA polymerase III, gamma/tau subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MNIALKHRPSSFKDLVGQDILVRVLENAFILNKIPQSILLSGSSGVGKTTTARIIALCLNCSLGPIFEPCGSCENCLAIK
NSSHPDVIEIDAASHTSIDDIKVILGDICYSPISSKFKVYIIDEVHMLSSSAFNALLKTLEEPPSSVKFILATTEIKKIP
ITIIARCQRFDLHNIPVAKIVERLNDVAQKESYSIEKGALELIARHSGNSMRNALFLMNQAVLYSKDGAISTKNVTDILG
LVDKDIIFDLLGAILEGDLQKALSVFDKAVKTANPLSIFEGLLQTIQLVCRFLITKEIDNAVTEHEKNRIKDLSIKRSLI
FFSRLWKMLLKGIQDIKTSTCNDVAAEMMLISLCYLSDLPSPEQVIKKVLSQNVEQGNRQGRPVAPSPSVIQVADYSDPK
KYVESKQQPHSVIPVRDTGIHVENKQQNYDFDKILQLLRRSNQIYLYKQLCNNLQLIDCKPGYLKLKAVSKLDSNFCNDL
KNYLNQVTQQEWVIAVDTGYINREVSNLNYAPAVKDILDTFKGAKVVNIENKE

Sequences:

>Translated_533_residues
MNIALKHRPSSFKDLVGQDILVRVLENAFILNKIPQSILLSGSSGVGKTTTARIIALCLNCSLGPIFEPCGSCENCLAIK
NSSHPDVIEIDAASHTSIDDIKVILGDICYSPISSKFKVYIIDEVHMLSSSAFNALLKTLEEPPSSVKFILATTEIKKIP
ITIIARCQRFDLHNIPVAKIVERLNDVAQKESYSIEKGALELIARHSGNSMRNALFLMNQAVLYSKDGAISTKNVTDILG
LVDKDIIFDLLGAILEGDLQKALSVFDKAVKTANPLSIFEGLLQTIQLVCRFLITKEIDNAVTEHEKNRIKDLSIKRSLI
FFSRLWKMLLKGIQDIKTSTCNDVAAEMMLISLCYLSDLPSPEQVIKKVLSQNVEQGNRQGRPVAPSPSVIQVADYSDPK
KYVESKQQPHSVIPVRDTGIHVENKQQNYDFDKILQLLRRSNQIYLYKQLCNNLQLIDCKPGYLKLKAVSKLDSNFCNDL
KNYLNQVTQQEWVIAVDTGYINREVSNLNYAPAVKDILDTFKGAKVVNIENKE
>Mature_533_residues
MNIALKHRPSSFKDLVGQDILVRVLENAFILNKIPQSILLSGSSGVGKTTTARIIALCLNCSLGPIFEPCGSCENCLAIK
NSSHPDVIEIDAASHTSIDDIKVILGDICYSPISSKFKVYIIDEVHMLSSSAFNALLKTLEEPPSSVKFILATTEIKKIP
ITIIARCQRFDLHNIPVAKIVERLNDVAQKESYSIEKGALELIARHSGNSMRNALFLMNQAVLYSKDGAISTKNVTDILG
LVDKDIIFDLLGAILEGDLQKALSVFDKAVKTANPLSIFEGLLQTIQLVCRFLITKEIDNAVTEHEKNRIKDLSIKRSLI
FFSRLWKMLLKGIQDIKTSTCNDVAAEMMLISLCYLSDLPSPEQVIKKVLSQNVEQGNRQGRPVAPSPSVIQVADYSDPK
KYVESKQQPHSVIPVRDTGIHVENKQQNYDFDKILQLLRRSNQIYLYKQLCNNLQLIDCKPGYLKLKAVSKLDSNFCNDL
KNYLNQVTQQEWVIAVDTGYINREVSNLNYAPAVKDILDTFKGAKVVNIENKE

Specific function: The gamma chain seems to interact with the delta subunit to transfer the beta subunit on the DNA [H]

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506491, Length=270, Percent_Identity=23.7037037037037, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI31881687, Length=270, Percent_Identity=23.7037037037037, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI6677723, Length=279, Percent_Identity=25.089605734767, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI194306571, Length=279, Percent_Identity=25.089605734767, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI194306567, Length=279, Percent_Identity=25.089605734767, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI31563534, Length=233, Percent_Identity=27.4678111587983, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1786676, Length=371, Percent_Identity=37.466307277628, Blast_Score=247, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17554730, Length=288, Percent_Identity=21.5277777777778, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17541988, Length=213, Percent_Identity=26.2910798122066, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322528, Length=281, Percent_Identity=24.9110320284698, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324039, Length=303, Percent_Identity=24.7524752475248, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18859927, Length=266, Percent_Identity=24.0601503759398, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17647857, Length=270, Percent_Identity=24.4444444444444, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR001270
- InterPro:   IPR008921
- InterPro:   IPR022001
- InterPro:   IPR022754
- InterPro:   IPR012763
- InterPro:   IPR021029 [H]

Pfam domain/function: PF00004 AAA; PF12169 DNA_pol3_gamma3; PF12168 DNA_pol3_tau_4; PF12170 DNA_pol3_tau_5 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 59494; Mature: 59494

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIALKHRPSSFKDLVGQDILVRVLENAFILNKIPQSILLSGSSGVGKTTTARIIALCLN
CCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCHHHHHHHHHHHC
CSLGPIFEPCGSCENCLAIKNSSHPDVIEIDAASHTSIDDIKVILGDICYSPISSKFKVY
CCCCCHHHHCCCCCCEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEE
IIDEVHMLSSSAFNALLKTLEEPPSSVKFILATTEIKKIPITIIARCQRFDLHNIPVAKI
EEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHCCHHHHHHHHHCCCCCCCHHHH
VERLNDVAQKESYSIEKGALELIARHSGNSMRNALFLMNQAVLYSKDGAISTKNVTDILG
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEECCCCCCCCHHHHHHH
LVDKDIIFDLLGAILEGDLQKALSVFDKAVKTANPLSIFEGLLQTIQLVCRFLITKEIDN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AVTEHEKNRIKDLSIKRSLIFFSRLWKMLLKGIQDIKTSTCNDVAAEMMLISLCYLSDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
SPEQVIKKVLSQNVEQGNRQGRPVAPSPSVIQVADYSDPKKYVESKQQPHSVIPVRDTGI
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCEEEEECCCC
HVENKQQNYDFDKILQLLRRSNQIYLYKQLCNNLQLIDCKPGYLKLKAVSKLDSNFCNDL
EECCCCCCCCHHHHHHHHHCCCCEEEEHHHHCCCEEEECCCCCEEHHHHHHHHHHHHHHH
KNYLNQVTQQEWVIAVDTGYINREVSNLNYAPAVKDILDTFKGAKVVNIENKE
HHHHHHHCCCCEEEEEECCCCCHHHCCCCCCHHHHHHHHHHCCCEEEECCCCC
>Mature Secondary Structure
MNIALKHRPSSFKDLVGQDILVRVLENAFILNKIPQSILLSGSSGVGKTTTARIIALCLN
CCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCHHHHHHHHHHHC
CSLGPIFEPCGSCENCLAIKNSSHPDVIEIDAASHTSIDDIKVILGDICYSPISSKFKVY
CCCCCHHHHCCCCCCEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEE
IIDEVHMLSSSAFNALLKTLEEPPSSVKFILATTEIKKIPITIIARCQRFDLHNIPVAKI
EEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHCCHHHHHHHHHCCCCCCCHHHH
VERLNDVAQKESYSIEKGALELIARHSGNSMRNALFLMNQAVLYSKDGAISTKNVTDILG
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEECCCCCCCCHHHHHHH
LVDKDIIFDLLGAILEGDLQKALSVFDKAVKTANPLSIFEGLLQTIQLVCRFLITKEIDN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AVTEHEKNRIKDLSIKRSLIFFSRLWKMLLKGIQDIKTSTCNDVAAEMMLISLCYLSDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
SPEQVIKKVLSQNVEQGNRQGRPVAPSPSVIQVADYSDPKKYVESKQQPHSVIPVRDTGI
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCEEEEECCCC
HVENKQQNYDFDKILQLLRRSNQIYLYKQLCNNLQLIDCKPGYLKLKAVSKLDSNFCNDL
EECCCCCCCCHHHHHHHHHCCCCEEEEHHHHCCCEEEECCCCCEEHHHHHHHHHHHHHHH
KNYLNQVTQQEWVIAVDTGYINREVSNLNYAPAVKDILDTFKGAKVVNIENKE
HHHHHHHCCCCEEEEEECCCCCHHHCCCCCCHHHHHHHHHHCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3534795; 3018672; 9278503; 2186364; 2187190; 2181440; 1575709 [H]