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The map label for this gene is folC [H]

Identifier: 225630786

GI number: 225630786

Start: 1180893

End: 1182182

Strand: Reverse

Name: folC [H]

Synonym: WRi_010890

Alternate gene names: 225630786

Gene position: 1182182-1180893 (Counterclockwise)

Preceding gene: 225630787

Following gene: 225630783

Centisome position: 81.76

GC content: 36.2

Gene sequence:

>1290_bases
ATGATATATATGCCTCATTGGCCTAAGCCACTTGGTAGCAGGCCGAATGATTTTTCTCTTGATCGCATAAAAAGCTTTCT
TAGTAAACTAGATAATCCCGAGAAAAAAGTGCCTCCCGTAATTCATATAGCTGGAACTAATGGGAAAGGCTCAACACTAG
CATTTATAAGATATATAATGCAAGCAGCAGGGTATAAGGTACACGCATACACTTCTCCACATTTGGTGAATTTTAATGAA
AGAATAGTTATTGCAGGCAGTTATATCGACGATAATGAATTATATAGCTTGTTAGAAGAATGCCGTGCCGTAATTGCAGA
ACAAGCTATCACTCTGTTTGAAGCTGCAACTATTGCAGCTTTTTTAGCTTTTTCTCGTCATAAAGCCGATATAACTTTAA
TTGAAGTGGGTATGGGTGGAAGACTTGATACAACAAATGTTATAGATAGTCCGATTTTAACAATAATTACTTCCATTGCA
CTTGATCATACGGAATATCTTGGCCCTACTATAGAAATTATAGCAGGTGAAAAGGCTGGAATAATGAAACCTAATGTTCC
TTGCGTGATAGCACCACAAGAAAAATCTATAATGAACACGCTAGAATACCACGCAATAAATAAGAAATCTCCCTTATATA
GAGGAGGTTTTGAATGGAATTGCGAAAAACAAGATAACAGGATGGTCTTTCAATCAGCTATTCAATCAATAGAATTTTCT
TTGCCGTCTTTAAAGGGTGATCACCAAATAATCAATGCAGGAAACGCCATTGCAGCATGTAGCATTTTGAGTGGAAAATA
TGGATTTGACATTGGAGAAGAGGATATCACTTCAGGTTTACAGTGCACTTATTGGCCTGCACGACTGGAGCGTATAAAAG
AGGGCAATTTAATTTCTTTATTACCAAAAGATTGGCAATTATTTTTGGATGGTGCGCACAATAATGATGGTGCTAGAGTA
CTATTTCAGTGGGTAAAAGATAATTTTGCTGAAGGTATCTATATAATTTTTGGTGTTACTCGTAACAGAAATGTGGAAGA
GTTCTTAGAACACTTAAAACCATATATCAAGTTATTATGTGCGGTTTGTGTTAAATCTGAGCCCAAAGCAACAAATACAA
ACTTGATTAGGGAAAAAGCCAATAACATAGGAATAAAAGCAATTGAGTGTGAGTCAATTGGCGATGCAATATCAAATCAC
ATATTAAAAGCCTCTATCCAAAACGTCAAAACCATACTGATCTGTGGCTCATTGTTCCTAGCTAGAGACTTCGCTATGGA
GAATAGCTAA

Upstream 100 bases:

>100_bases
GAATAAGTATTGCTATTAATCAACGTTCTCAATTTAGTGAGAAGATTGACACGCATGGCATAGCAAGTGTGATAGGTGAA
TTAAAATGTAACAATAATCT

Downstream 100 bases:

>100_bases
CTTTGATGATATTAACTGACCGATGGGGAAATAACTTTGATTGCTCATAGTACTGCTTGGCTTTTCGTTACCGACTTCTA
TAAACTGATCATCACCTTTA

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MIYMPHWPKPLGSRPNDFSLDRIKSFLSKLDNPEKKVPPVIHIAGTNGKGSTLAFIRYIMQAAGYKVHAYTSPHLVNFNE
RIVIAGSYIDDNELYSLLEECRAVIAEQAITLFEAATIAAFLAFSRHKADITLIEVGMGGRLDTTNVIDSPILTIITSIA
LDHTEYLGPTIEIIAGEKAGIMKPNVPCVIAPQEKSIMNTLEYHAINKKSPLYRGGFEWNCEKQDNRMVFQSAIQSIEFS
LPSLKGDHQIINAGNAIAACSILSGKYGFDIGEEDITSGLQCTYWPARLERIKEGNLISLLPKDWQLFLDGAHNNDGARV
LFQWVKDNFAEGIYIIFGVTRNRNVEEFLEHLKPYIKLLCAVCVKSEPKATNTNLIREKANNIGIKAIECESIGDAISNH
ILKASIQNVKTILICGSLFLARDFAMENS

Sequences:

>Translated_429_residues
MIYMPHWPKPLGSRPNDFSLDRIKSFLSKLDNPEKKVPPVIHIAGTNGKGSTLAFIRYIMQAAGYKVHAYTSPHLVNFNE
RIVIAGSYIDDNELYSLLEECRAVIAEQAITLFEAATIAAFLAFSRHKADITLIEVGMGGRLDTTNVIDSPILTIITSIA
LDHTEYLGPTIEIIAGEKAGIMKPNVPCVIAPQEKSIMNTLEYHAINKKSPLYRGGFEWNCEKQDNRMVFQSAIQSIEFS
LPSLKGDHQIINAGNAIAACSILSGKYGFDIGEEDITSGLQCTYWPARLERIKEGNLISLLPKDWQLFLDGAHNNDGARV
LFQWVKDNFAEGIYIIFGVTRNRNVEEFLEHLKPYIKLLCAVCVKSEPKATNTNLIREKANNIGIKAIECESIGDAISNH
ILKASIQNVKTILICGSLFLARDFAMENS
>Mature_429_residues
MIYMPHWPKPLGSRPNDFSLDRIKSFLSKLDNPEKKVPPVIHIAGTNGKGSTLAFIRYIMQAAGYKVHAYTSPHLVNFNE
RIVIAGSYIDDNELYSLLEECRAVIAEQAITLFEAATIAAFLAFSRHKADITLIEVGMGGRLDTTNVIDSPILTIITSIA
LDHTEYLGPTIEIIAGEKAGIMKPNVPCVIAPQEKSIMNTLEYHAINKKSPLYRGGFEWNCEKQDNRMVFQSAIQSIEFS
LPSLKGDHQIINAGNAIAACSILSGKYGFDIGEEDITSGLQCTYWPARLERIKEGNLISLLPKDWQLFLDGAHNNDGARV
LFQWVKDNFAEGIYIIFGVTRNRNVEEFLEHLKPYIKLLCAVCVKSEPKATNTNLIREKANNIGIKAIECESIGDAISNH
ILKASIQNVKTILICGSLFLARDFAMENS

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=357, Percent_Identity=29.4117647058824, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI66932990, Length=357, Percent_Identity=29.4117647058824, Blast_Score=141, Evalue=1e-33,
Organism=Escherichia coli, GI1788654, Length=410, Percent_Identity=29.7560975609756, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI71984923, Length=338, Percent_Identity=26.9230769230769, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17553150, Length=340, Percent_Identity=27.0588235294118, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17553148, Length=338, Percent_Identity=26.9230769230769, Blast_Score=127, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6323760, Length=421, Percent_Identity=28.0285035629454, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6324815, Length=359, Percent_Identity=27.5766016713092, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24641571, Length=367, Percent_Identity=28.3378746594005, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24581568, Length=163, Percent_Identity=34.3558282208589, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47675; Mature: 47675

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYMPHWPKPLGSRPNDFSLDRIKSFLSKLDNPEKKVPPVIHIAGTNGKGSTLAFIRYIM
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHCCCEEEEECCCCCCHHHHHHHHHH
QAAGYKVHAYTSPHLVNFNERIVIAGSYIDDNELYSLLEECRAVIAEQAITLFEAATIAA
HHCCCEEEEECCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLAFSRHKADITLIEVGMGGRLDTTNVIDSPILTIITSIALDHTEYLGPTIEIIAGEKAG
HHHHHCCCCCEEEEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
IMKPNVPCVIAPQEKSIMNTLEYHAINKKSPLYRGGFEWNCEKQDNRMVFQSAIQSIEFS
CCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHC
LPSLKGDHQIINAGNAIAACSILSGKYGFDIGEEDITSGLQCTYWPARLERIKEGNLISL
CCCCCCCCEEEECCCCEEEEEEECCCCCCCCCHHHHHCCCEEEECHHHHHHHCCCCEEEE
LPKDWQLFLDGAHNNDGARVLFQWVKDNFAEGIYIIFGVTRNRNVEEFLEHLKPYIKLLC
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
AVCVKSEPKATNTNLIREKANNIGIKAIECESIGDAISNHILKASIQNVKTILICGSLFL
HHHHCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARDFAMENS
HHHHHCCCC
>Mature Secondary Structure
MIYMPHWPKPLGSRPNDFSLDRIKSFLSKLDNPEKKVPPVIHIAGTNGKGSTLAFIRYIM
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHCCCEEEEECCCCCCHHHHHHHHHH
QAAGYKVHAYTSPHLVNFNERIVIAGSYIDDNELYSLLEECRAVIAEQAITLFEAATIAA
HHCCCEEEEECCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLAFSRHKADITLIEVGMGGRLDTTNVIDSPILTIITSIALDHTEYLGPTIEIIAGEKAG
HHHHHCCCCCEEEEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
IMKPNVPCVIAPQEKSIMNTLEYHAINKKSPLYRGGFEWNCEKQDNRMVFQSAIQSIEFS
CCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHC
LPSLKGDHQIINAGNAIAACSILSGKYGFDIGEEDITSGLQCTYWPARLERIKEGNLISL
CCCCCCCCEEEECCCCEEEEEEECCCCCCCCCHHHHHCCCEEEECHHHHHHHCCCCEEEE
LPKDWQLFLDGAHNNDGARVLFQWVKDNFAEGIYIIFGVTRNRNVEEFLEHLKPYIKLLC
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
AVCVKSEPKATNTNLIREKANNIGIKAIECESIGDAISNHILKASIQNVKTILICGSLFL
HHHHCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARDFAMENS
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]