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The map label for this gene is glyA [H]

Identifier: 225630769

GI number: 225630769

Start: 1152950

End: 1154227

Strand: Direct

Name: glyA [H]

Synonym: WRi_010650

Alternate gene names: 225630769

Gene position: 1152950-1154227 (Clockwise)

Preceding gene: 225630768

Following gene: 225630770

Centisome position: 79.74

GC content: 39.44

Gene sequence:

>1278_bases
ATGATGAGTGTTTTAAAAAAAATCTGTGGCTCTAAAAATAGTTTAAAGTCTTTTGATAACGAGGTTTATCAGTCTATAGA
AAAAGAATTACAACGCCAAAAATCACAATTGCAATTAATTGCATCAGAAAATTTTGCAAGCAAAGCGGTAATGGAGGCAC
AAGGCTCTTTTCTGACTAATAAATACGCAGAAGGTTATCCAGGTAAAAGATATTACTGTGGTTGTGAGCATGTGGACAAA
ATTGAAAGTCTGGCTATAGAAAGACTTTGTAAGTTGTTTGGTGTTAAATTTGCAAACGTTCAACCTCATTCTGGTTCTCA
GGCAAACCAGGCGGTATTTGCTTCACTGCTTACTCCAGGCGATACAATACTTGGATTATCACTGAGTTGCGGTGGGCATC
TAACTCATGGTGCGGCACCAAGCCTTTCTGGTAAATGGTTTAAGTCAATTCAATATACAGTGAATAAAGACACTTACTTG
CTGGATATGGATGAGATAGAAAAGCTGGCGCTGGAGCATAAACCGAAATTGATCATAGCTGGTGCTTCTGCTTATCCGAG
AAAAATGGACTTCAAACGCTTTCGCGAAATTGCAGATAAAGTTGGTGCTTATTTGCTTGCAGACATTGCTCACTATGCAG
GGCTTATTGCAGCAGGTGAATATCCGTCTCCTGCTGAATATGCACATGTTATGACTTCCACAACTCACAAAACTTTGCGT
GGTCCTCGTGGTGGAATAGTGATGACCAATGATGAAATATTACACAAAAAAATTCAATCTGCAGTTTTTCCAGGATTGCA
GGGCGGGCCACTTATGCATGTGATAGCTGCAAAAGCTGTTGCATTTAAAGAAGCATTGGCGCCAGAATTTAAAACTTATA
GCAAGAAAGTCGTGGAAAATGCGAAAGTACTAGCTCAAGAATTGCAAAAGCATGGACTCGACATTATAACCGGTGGCACT
GACTCTCATATAGTGCTAGTTGACTTAAGATCGCAGAAATTAACTGGAAAAGACGTTGTAGATAGCCTTGAGAGAGCCGG
TATTACCTGTAATAAAAACTCTGTACCATTTGATACAGCAAAGCCGACCATCACTTCAGGGCTCCGTTTTGGCACCGCTG
CTGAGACAACACGCGGACTTGAGGCAGAAAATTTTAAGGAGATAGCTGATCTAATAAATGAAGTAATTCAAGGATTAATC
AGCGGAAATAGCTCAAGTGTCGAAAAAGCAGTAAAAGCTAAAGTTGAAAGGATTTGTAGTAATTTTCCTATTTATTAA

Upstream 100 bases:

>100_bases
TGTTTGCTGATAGCTGTTTTTGGGCTCTTATGGTTAAAAAAGCAGGATAAGCAGTCTTAAAATTGCTTGTGCATCTTAAA
ATTAAAGCGAGGTTTGATCT

Downstream 100 bases:

>100_bases
TTTATGTGGTGTCATCCAAGTAGCTGGTAGAAACGAAAAACTTCCTTGACAAACTCCGCCAGCCCCCTTATCATGAAACT
GAAGCTATTGTATTTGCTTT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MMSVLKKICGSKNSLKSFDNEVYQSIEKELQRQKSQLQLIASENFASKAVMEAQGSFLTNKYAEGYPGKRYYCGCEHVDK
IESLAIERLCKLFGVKFANVQPHSGSQANQAVFASLLTPGDTILGLSLSCGGHLTHGAAPSLSGKWFKSIQYTVNKDTYL
LDMDEIEKLALEHKPKLIIAGASAYPRKMDFKRFREIADKVGAYLLADIAHYAGLIAAGEYPSPAEYAHVMTSTTHKTLR
GPRGGIVMTNDEILHKKIQSAVFPGLQGGPLMHVIAAKAVAFKEALAPEFKTYSKKVVENAKVLAQELQKHGLDIITGGT
DSHIVLVDLRSQKLTGKDVVDSLERAGITCNKNSVPFDTAKPTITSGLRFGTAAETTRGLEAENFKEIADLINEVIQGLI
SGNSSSVEKAVKAKVERICSNFPIY

Sequences:

>Translated_425_residues
MMSVLKKICGSKNSLKSFDNEVYQSIEKELQRQKSQLQLIASENFASKAVMEAQGSFLTNKYAEGYPGKRYYCGCEHVDK
IESLAIERLCKLFGVKFANVQPHSGSQANQAVFASLLTPGDTILGLSLSCGGHLTHGAAPSLSGKWFKSIQYTVNKDTYL
LDMDEIEKLALEHKPKLIIAGASAYPRKMDFKRFREIADKVGAYLLADIAHYAGLIAAGEYPSPAEYAHVMTSTTHKTLR
GPRGGIVMTNDEILHKKIQSAVFPGLQGGPLMHVIAAKAVAFKEALAPEFKTYSKKVVENAKVLAQELQKHGLDIITGGT
DSHIVLVDLRSQKLTGKDVVDSLERAGITCNKNSVPFDTAKPTITSGLRFGTAAETTRGLEAENFKEIADLINEVIQGLI
SGNSSSVEKAVKAKVERICSNFPIY
>Mature_425_residues
MMSVLKKICGSKNSLKSFDNEVYQSIEKELQRQKSQLQLIASENFASKAVMEAQGSFLTNKYAEGYPGKRYYCGCEHVDK
IESLAIERLCKLFGVKFANVQPHSGSQANQAVFASLLTPGDTILGLSLSCGGHLTHGAAPSLSGKWFKSIQYTVNKDTYL
LDMDEIEKLALEHKPKLIIAGASAYPRKMDFKRFREIADKVGAYLLADIAHYAGLIAAGEYPSPAEYAHVMTSTTHKTLR
GPRGGIVMTNDEILHKKIQSAVFPGLQGGPLMHVIAAKAVAFKEALAPEFKTYSKKVVENAKVLAQELQKHGLDIITGGT
DSHIVLVDLRSQKLTGKDVVDSLERAGITCNKNSVPFDTAKPTITSGLRFGTAAETTRGLEAENFKEIADLINEVIQGLI
SGNSSSVEKAVKAKVERICSNFPIY

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=406, Percent_Identity=46.0591133004926, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI261862352, Length=406, Percent_Identity=46.0591133004926, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI261862350, Length=406, Percent_Identity=46.0591133004926, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI261862348, Length=406, Percent_Identity=46.0591133004926, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI22547186, Length=433, Percent_Identity=46.6512702078522, Blast_Score=338, Evalue=6e-93,
Organism=Homo sapiens, GI261862346, Length=406, Percent_Identity=45.320197044335, Blast_Score=328, Evalue=4e-90,
Organism=Homo sapiens, GI22547189, Length=419, Percent_Identity=43.6754176610979, Blast_Score=302, Evalue=5e-82,
Organism=Escherichia coli, GI1788902, Length=414, Percent_Identity=53.6231884057971, Blast_Score=436, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI25144732, Length=409, Percent_Identity=45.721271393643, Blast_Score=358, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI25144729, Length=409, Percent_Identity=45.721271393643, Blast_Score=358, Evalue=3e-99,
Organism=Saccharomyces cerevisiae, GI6319739, Length=417, Percent_Identity=44.1247002398081, Blast_Score=348, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=46.2686567164179, Blast_Score=345, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24640005, Length=420, Percent_Identity=46.6666666666667, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221329721, Length=408, Percent_Identity=47.0588235294118, Blast_Score=359, Evalue=2e-99,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 46319; Mature: 46319

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSVLKKICGSKNSLKSFDNEVYQSIEKELQRQKSQLQLIASENFASKAVMEAQGSFLTN
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
KYAEGYPGKRYYCGCEHVDKIESLAIERLCKLFGVKFANVQPHSGSQANQAVFASLLTPG
HHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCHHHHHHHHHHCCC
DTILGLSLSCGGHLTHGAAPSLSGKWFKSIQYTVNKDTYLLDMDEIEKLALEHKPKLIIA
CCEEEEEECCCCCCCCCCCCCCCCHHHHHHHEEECCCEEEEEHHHHHHHHHHCCCCEEEE
GASAYPRKMDFKRFREIADKVGAYLLADIAHYAGLIAAGEYPSPAEYAHVMTSTTHKTLR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
GPRGGIVMTNDEILHKKIQSAVFPGLQGGPLMHVIAAKAVAFKEALAPEFKTYSKKVVEN
CCCCCEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
AKVLAQELQKHGLDIITGGTDSHIVLVDLRSQKLTGKDVVDSLERAGITCNKNSVPFDTA
HHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEECCCCCCCCCC
KPTITSGLRFGTAAETTRGLEAENFKEIADLINEVIQGLISGNSSSVEKAVKAKVERICS
CCHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NFPIY
CCCCC
>Mature Secondary Structure
MMSVLKKICGSKNSLKSFDNEVYQSIEKELQRQKSQLQLIASENFASKAVMEAQGSFLTN
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
KYAEGYPGKRYYCGCEHVDKIESLAIERLCKLFGVKFANVQPHSGSQANQAVFASLLTPG
HHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCHHHHHHHHHHCCC
DTILGLSLSCGGHLTHGAAPSLSGKWFKSIQYTVNKDTYLLDMDEIEKLALEHKPKLIIA
CCEEEEEECCCCCCCCCCCCCCCCHHHHHHHEEECCCEEEEEHHHHHHHHHHCCCCEEEE
GASAYPRKMDFKRFREIADKVGAYLLADIAHYAGLIAAGEYPSPAEYAHVMTSTTHKTLR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
GPRGGIVMTNDEILHKKIQSAVFPGLQGGPLMHVIAAKAVAFKEALAPEFKTYSKKVVEN
CCCCCEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
AKVLAQELQKHGLDIITGGTDSHIVLVDLRSQKLTGKDVVDSLERAGITCNKNSVPFDTA
HHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEECCCCCCCCCC
KPTITSGLRFGTAAETTRGLEAENFKEIADLINEVIQGLISGNSSSVEKAVKAKVERICS
CCHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NFPIY
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA