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The map label for this gene is topA [H]

Identifier: 225630766

GI number: 225630766

Start: 1145928

End: 1148378

Strand: Reverse

Name: topA [H]

Synonym: WRi_010610

Alternate gene names: 225630766

Gene position: 1148378-1145928 (Counterclockwise)

Preceding gene: 225630771

Following gene: 225630764

Centisome position: 79.42

GC content: 37.05

Gene sequence:

>2451_bases
ATGGCATTATTAATAGTTGAATCACCTGCGAAAGCAAAGACAATAGGTAAATATTTGGGTAAAGAGTTCAAAGTAGCTGC
ATCCTTTGGACATGTGAGGGATCTTCCAGCAAAAAACGGTTCTGTCGATCCGGATAATGATTTTGCTATAAAGTATGAAA
CCATTGAAAAAGCAGAAAAGTATATAAAAGAGTTAGTAAAAGCCGCAAGCAAAACATCAGATATATATCTTGCAACAGAC
CCAGACAGAGAAGGGGAAGCAATGGCTTGGCATGTGATAGAGACATTAAAAGAAAAGAAAGCAATCAGTAATGAAAACAA
CATTTATAGAGTAGTCTTTAATGAGATAACAAAGAGAGCAGTTCAAGAAGCAATAAAAAATCCACGTGAAATTAATATGG
ATTTAGTGCGTGCACAACAAGCACGTAGAGCTTTGGATTATCTAGTTGGATTTAGTTTGTCACCACTGCTGTGGACAAAA
TTGTCGGGAAGCAAGTCTGCAGGGCGAGTGCAGTCTGTTGCATTAAAGATTATATGCGAACGAGAAGATGAAATTAGTAA
ATTTATAACACAGGAGTATTGGAGCATAAAAGCAGAAATGCAAAATAGCAAAGATGAGGCTTTTTTTGCTATGCTAAGCC
ACTATGACAATAAAAAGCTAGAAAAATTTGATATTAAGAATGAAGAAGAAGCAAAGAACTTAGTCAGGGAGATTGAATCA
AGGCAATATGCTGTAAGCACAGTAGAACGCAAGCAAGTTAAGAGAAACCCGCTTCCTCCGTTTATTACTTCAAGTCTTCA
GCAAGATGCAGTGAATAAACTGTATTTTAATGTGAAAAGTGTTATGCGCATAGCGCAAAATTTATATGAAGGTATCGATA
TTGGTGGTGAAACTGTAGGGTTGATAACTTACATGCGTACAGATGGGTTTCATATTGCAGATGAGGCTATAAACTCAATT
AGAGGGTCAATTAAGTCATTATATGGTGATAAATATTTACCGCAGTCTCCTCGTAAATATGTAAAAAAGGTCAAAAATGC
TCAAGAAGCACATGAAGCAATTCGTCCAACTGATATCAATAGAACGCCGGGTAGTATTAAGGATTACTTAACGCCAGAGC
AATTTAAATTGTACGATTTAATCTGGAAAAGGACCATTGCAAGTCAAATGGAATCGGCGATTCTTGATCAAGTGGTAGTT
GAAATTAGTTCTACTGATCAAAAAGTAGTTCTGCGAGCAAGTGGGTCGAGTATATTCTTTGATGGTTTTTATAGAGTCTA
TCAAGATAACATGGAAGCCGAAAATGAAGGCCTGCTACCTGCCATGAAGGAAGGGGAAGCATGCAAGCTGATTTCAGTTG
AGCCAAAACAGCATTTCACTCAACCGCCACCACGTTACAGTGAAGCGAGTATCGTAAAAAAAATGGAAGAAATCGGTATA
GGTCGACCATCAACTTATGCAACAATTATTTCGGTATTACAAGATCGTGAGTACGTTTCATTGGATAGCAAAAGGTTTAT
TCCAAGCAGTCGTGGTAAAATCGTTACTATATTTTTAGAAACTTTTTTTCAGCGTTGTGTAGAGTATGATTTCACAGCAC
AAATGGAAGAAAAGCTTGATTTAATTTCAAATGGACATGCAGATTGGAAAAAAGAATTAGGCCACTTTTGGGTGCCATTT
TTTGGTCATGTAAACTCTGTCAAGCAAATGACGCATGATGAAGTTTTCAGCGGCATTCACAATTTGGTAGTCGATTGGTT
TTGTTCAGAAGAAGGAAGAAAAGAGGTGAATACGAAATGCCCTGCTTGCTTAGGTGGCATATTGAAATTGAACTTTGGAA
AAGCCGGGGTGTTCCTTGGATGTTCTAACTATCCTGAATGCAACCATACAAAAGAAATTACAGGTAGTAACGACAATTCA
GAATATCCAAAAAGTTTAGGTGTAGATGGTATAACAAACCAAGAAGTGATGATCAAAAAGGGTCCTTTTGGACTTTATCT
GGAGTTTAATAACGAGTTAAAAAAGAAAAAAACGGTTTCCATACCAAAAGATATAAATGTTAATGAGATTGATCTGAGCA
CCGCTACTCAGTTACTTTCCTTGCCAAAAGTAATCGGAGAACACCCTGAAACCGGAAAGGAAGTAAAAATAGGCCTTGGG
CGATTTGGGTACTATATTTTCTATGATGGTAAATACTTTTCTCTTAAGAAAAGCTCCAAAGAGGTGCTGAATACAGAATT
GAGCGAAGCTGTACAAATTATTGCAAACAGTCCACGCAAAGAGTTAAAATCTCTCGGGGTTAATGAAAAGGGAAAAGAAG
TTTTTATCTGCAATGGTAGGTATGGATTCTATATAAAATGTGGTAAAACAAACGTTGCTTTGGGCAAGAGTGCAGATATT
GAGAGCATAGATCTGGAGAAGGCTTTAGAGTTGATTAAGGGCAAGAAATAG

Upstream 100 bases:

>100_bases
ACCAGGTTCGTAAAATTCACAAGAACCATTAAAATATTTTTTTACTTTTCATCGTATATTTCTCAACATATTATCTTTAA
GTACTAAAATAATTAACAAT

Downstream 100 bases:

>100_bases
ACTTCTTGCATAACCATTATCTACGTTAAAAATCAGCTTTTTTGTCATTCCAGCGTGTGACGCTGGAATCTAGAAAAAAA
GAACCTGGATGCCAGTGTCT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 816; Mature: 815

Protein sequence:

>816_residues
MALLIVESPAKAKTIGKYLGKEFKVAASFGHVRDLPAKNGSVDPDNDFAIKYETIEKAEKYIKELVKAASKTSDIYLATD
PDREGEAMAWHVIETLKEKKAISNENNIYRVVFNEITKRAVQEAIKNPREINMDLVRAQQARRALDYLVGFSLSPLLWTK
LSGSKSAGRVQSVALKIICEREDEISKFITQEYWSIKAEMQNSKDEAFFAMLSHYDNKKLEKFDIKNEEEAKNLVREIES
RQYAVSTVERKQVKRNPLPPFITSSLQQDAVNKLYFNVKSVMRIAQNLYEGIDIGGETVGLITYMRTDGFHIADEAINSI
RGSIKSLYGDKYLPQSPRKYVKKVKNAQEAHEAIRPTDINRTPGSIKDYLTPEQFKLYDLIWKRTIASQMESAILDQVVV
EISSTDQKVVLRASGSSIFFDGFYRVYQDNMEAENEGLLPAMKEGEACKLISVEPKQHFTQPPPRYSEASIVKKMEEIGI
GRPSTYATIISVLQDREYVSLDSKRFIPSSRGKIVTIFLETFFQRCVEYDFTAQMEEKLDLISNGHADWKKELGHFWVPF
FGHVNSVKQMTHDEVFSGIHNLVVDWFCSEEGRKEVNTKCPACLGGILKLNFGKAGVFLGCSNYPECNHTKEITGSNDNS
EYPKSLGVDGITNQEVMIKKGPFGLYLEFNNELKKKKTVSIPKDINVNEIDLSTATQLLSLPKVIGEHPETGKEVKIGLG
RFGYYIFYDGKYFSLKKSSKEVLNTELSEAVQIIANSPRKELKSLGVNEKGKEVFICNGRYGFYIKCGKTNVALGKSADI
ESIDLEKALELIKGKK

Sequences:

>Translated_816_residues
MALLIVESPAKAKTIGKYLGKEFKVAASFGHVRDLPAKNGSVDPDNDFAIKYETIEKAEKYIKELVKAASKTSDIYLATD
PDREGEAMAWHVIETLKEKKAISNENNIYRVVFNEITKRAVQEAIKNPREINMDLVRAQQARRALDYLVGFSLSPLLWTK
LSGSKSAGRVQSVALKIICEREDEISKFITQEYWSIKAEMQNSKDEAFFAMLSHYDNKKLEKFDIKNEEEAKNLVREIES
RQYAVSTVERKQVKRNPLPPFITSSLQQDAVNKLYFNVKSVMRIAQNLYEGIDIGGETVGLITYMRTDGFHIADEAINSI
RGSIKSLYGDKYLPQSPRKYVKKVKNAQEAHEAIRPTDINRTPGSIKDYLTPEQFKLYDLIWKRTIASQMESAILDQVVV
EISSTDQKVVLRASGSSIFFDGFYRVYQDNMEAENEGLLPAMKEGEACKLISVEPKQHFTQPPPRYSEASIVKKMEEIGI
GRPSTYATIISVLQDREYVSLDSKRFIPSSRGKIVTIFLETFFQRCVEYDFTAQMEEKLDLISNGHADWKKELGHFWVPF
FGHVNSVKQMTHDEVFSGIHNLVVDWFCSEEGRKEVNTKCPACLGGILKLNFGKAGVFLGCSNYPECNHTKEITGSNDNS
EYPKSLGVDGITNQEVMIKKGPFGLYLEFNNELKKKKTVSIPKDINVNEIDLSTATQLLSLPKVIGEHPETGKEVKIGLG
RFGYYIFYDGKYFSLKKSSKEVLNTELSEAVQIIANSPRKELKSLGVNEKGKEVFICNGRYGFYIKCGKTNVALGKSADI
ESIDLEKALELIKGKK
>Mature_815_residues
ALLIVESPAKAKTIGKYLGKEFKVAASFGHVRDLPAKNGSVDPDNDFAIKYETIEKAEKYIKELVKAASKTSDIYLATDP
DREGEAMAWHVIETLKEKKAISNENNIYRVVFNEITKRAVQEAIKNPREINMDLVRAQQARRALDYLVGFSLSPLLWTKL
SGSKSAGRVQSVALKIICEREDEISKFITQEYWSIKAEMQNSKDEAFFAMLSHYDNKKLEKFDIKNEEEAKNLVREIESR
QYAVSTVERKQVKRNPLPPFITSSLQQDAVNKLYFNVKSVMRIAQNLYEGIDIGGETVGLITYMRTDGFHIADEAINSIR
GSIKSLYGDKYLPQSPRKYVKKVKNAQEAHEAIRPTDINRTPGSIKDYLTPEQFKLYDLIWKRTIASQMESAILDQVVVE
ISSTDQKVVLRASGSSIFFDGFYRVYQDNMEAENEGLLPAMKEGEACKLISVEPKQHFTQPPPRYSEASIVKKMEEIGIG
RPSTYATIISVLQDREYVSLDSKRFIPSSRGKIVTIFLETFFQRCVEYDFTAQMEEKLDLISNGHADWKKELGHFWVPFF
GHVNSVKQMTHDEVFSGIHNLVVDWFCSEEGRKEVNTKCPACLGGILKLNFGKAGVFLGCSNYPECNHTKEITGSNDNSE
YPKSLGVDGITNQEVMIKKGPFGLYLEFNNELKKKKTVSIPKDINVNEIDLSTATQLLSLPKVIGEHPETGKEVKIGLGR
FGYYIFYDGKYFSLKKSSKEVLNTELSEAVQIIANSPRKELKSLGVNEKGKEVFICNGRYGFYIKCGKTNVALGKSADIE
SIDLEKALELIKGKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=621, Percent_Identity=22.7053140096618, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI10835218, Length=620, Percent_Identity=24.3548387096774, Blast_Score=113, Evalue=9e-25,
Organism=Escherichia coli, GI1787529, Length=654, Percent_Identity=41.4373088685015, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1788061, Length=538, Percent_Identity=25.2788104089219, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32563869, Length=562, Percent_Identity=24.9110320284698, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=483, Percent_Identity=24.6376811594203, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=560, Percent_Identity=23.0357142857143, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585251, Length=624, Percent_Identity=22.5961538461538, Blast_Score=101, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 92262; Mature: 92130

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLIVESPAKAKTIGKYLGKEFKVAASFGHVRDLPAKNGSVDPDNDFAIKYETIEKAEK
CEEEEECCCHHHHHHHHHHCCCEEEHHHCCCHHCCCCCCCCCCCCCCCEEEHHHHHHHHH
YIKELVKAASKTSDIYLATDPDREGEAMAWHVIETLKEKKAISNENNIYRVVFNEITKRA
HHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHH
VQEAIKNPREINMDLVRAQQARRALDYLVGFSLSPLLWTKLSGSKSAGRVQSVALKIICE
HHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCHHHHHHHHHHHC
REDEISKFITQEYWSIKAEMQNSKDEAFFAMLSHYDNKKLEKFDIKNEEEAKNLVREIES
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHH
RQYAVSTVERKQVKRNPLPPFITSSLQQDAVNKLYFNVKSVMRIAQNLYEGIDIGGETVG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
LITYMRTDGFHIADEAINSIRGSIKSLYGDKYLPQSPRKYVKKVKNAQEAHEAIRPTDIN
EEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
RTPGSIKDYLTPEQFKLYDLIWKRTIASQMESAILDQVVVEISSTDQKVVLRASGSSIFF
CCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEH
DGFYRVYQDNMEAENEGLLPAMKEGEACKLISVEPKQHFTQPPPRYSEASIVKKMEEIGI
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHHCC
GRPSTYATIISVLQDREYVSLDSKRFIPSSRGKIVTIFLETFFQRCVEYDFTAQMEEKLD
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHH
LISNGHADWKKELGHFWVPFFGHVNSVKQMTHDEVFSGIHNLVVDWFCSEEGRKEVNTKC
HHHCCCCHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCC
PACLGGILKLNFGKAGVFLGCSNYPECNHTKEITGSNDNSEYPKSLGVDGITNQEVMIKK
HHHHCCHHEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEEE
GPFGLYLEFNNELKKKKTVSIPKDINVNEIDLSTATQLLSLPKVIGEHPETGKEVKIGLG
CCEEEEEEECCCHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
RFGYYIFYDGKYFSLKKSSKEVLNTELSEAVQIIANSPRKELKSLGVNEKGKEVFICNGR
CEEEEEEECCEEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEECCC
YGFYIKCGKTNVALGKSADIESIDLEKALELIKGKK
EEEEEEECCCEEECCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
ALLIVESPAKAKTIGKYLGKEFKVAASFGHVRDLPAKNGSVDPDNDFAIKYETIEKAEK
EEEEECCCHHHHHHHHHHCCCEEEHHHCCCHHCCCCCCCCCCCCCCCEEEHHHHHHHHH
YIKELVKAASKTSDIYLATDPDREGEAMAWHVIETLKEKKAISNENNIYRVVFNEITKRA
HHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHH
VQEAIKNPREINMDLVRAQQARRALDYLVGFSLSPLLWTKLSGSKSAGRVQSVALKIICE
HHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCHHHHHHHHHHHC
REDEISKFITQEYWSIKAEMQNSKDEAFFAMLSHYDNKKLEKFDIKNEEEAKNLVREIES
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHH
RQYAVSTVERKQVKRNPLPPFITSSLQQDAVNKLYFNVKSVMRIAQNLYEGIDIGGETVG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
LITYMRTDGFHIADEAINSIRGSIKSLYGDKYLPQSPRKYVKKVKNAQEAHEAIRPTDIN
EEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
RTPGSIKDYLTPEQFKLYDLIWKRTIASQMESAILDQVVVEISSTDQKVVLRASGSSIFF
CCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEH
DGFYRVYQDNMEAENEGLLPAMKEGEACKLISVEPKQHFTQPPPRYSEASIVKKMEEIGI
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHHCC
GRPSTYATIISVLQDREYVSLDSKRFIPSSRGKIVTIFLETFFQRCVEYDFTAQMEEKLD
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHH
LISNGHADWKKELGHFWVPFFGHVNSVKQMTHDEVFSGIHNLVVDWFCSEEGRKEVNTKC
HHHCCCCHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCC
PACLGGILKLNFGKAGVFLGCSNYPECNHTKEITGSNDNSEYPKSLGVDGITNQEVMIKK
HHHHCCHHEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEEE
GPFGLYLEFNNELKKKKTVSIPKDINVNEIDLSTATQLLSLPKVIGEHPETGKEVKIGLG
CCEEEEEEECCCHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
RFGYYIFYDGKYFSLKKSSKEVLNTELSEAVQIIANSPRKELKSLGVNEKGKEVFICNGR
CEEEEEEECCEEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEECCC
YGFYIKCGKTNVALGKSADIESIDLEKALELIKGKK
EEEEEEECCCEEECCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA